Structural analysis and proteolytic activation of Enterococcus faecalis cytolysin, a novel lantibiotic

Molecular Microbiology

Volumn 21, Issue 6, 1996, Pages 1175-1184

  Booth, Mary C ^a   Bogie, Charles P ^b   Sahl, Hans Georg ^c   Siezen, Roland J ^d   Hatter, Kenneth L ^a   Gilmore, Michael S ^a,b  

^a UNIVERSITY OF OKLAHOMA HEALTH SCIENCES CENTER   (United States)

^b UNIVERSITY OF OKLAHOMA   (United States)

^c UNIVERSITY OF BONN   (Germany)

^d NIZO FOOD RESEARCH   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIOCIN; CYTOLYSIN; LANTIBIOTIC;

ANTIMICROBIAL ACTIVITY; ARTICLE; CONTROLLED STUDY; ENTEROCOCCUS; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DEGRADATION; PROTEIN PROCESSING; PROTEIN PURIFICATION;

ANIMALIA; ENTEROCOCCUS; ENTEROCOCCUS FAECALIS; EUKARYOTA; PROKARYOTA;

EID: 0029786349     PISSN: 0950382X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2958.1996.831449.x     Document Type: Article

References (45)

1. Abee, T., Klaenhammer, T.R., and Letellier, L. (1994) Kinetic studies of the action of lactacin F, a bacteriocin produced by Lactobacillus johnsonii that forms poration complexes in the cytoplasmic membrane. Appl Environ Microbiol 60: 1006-1013.
2. Blundell, T., Carney, D., Gardner, S., Hayes, F., Howlin, B., Overington, J., Singh, D.A., Sibande, B.L., and Sutcliffe, M. (1988) Knowledge based protein modelling and design. Eur J Biochem 172: 513-520.
3. Brock, T.D., and Davie, J.M. (1963) Probable identity of a group D hemolysin with a bacteriocine. J Bacteriol 86: 708-712.
4. Brooks, B.R., Bruccoleri, R.E., Olafson, B.D., States, D.J., Swaninathan, S., and Karplus, M. (1983) CHARM: a program for macromolecular energy minimization and dynamics calculations. J Comp Chem 4: 187-217.
5. Clewell, D.B., Tomich, P.K., Gawron-Burke, M.C., Franke, A.E., Yagi, Y., and An, F.Y. (1982) Mapping of Streptococcus faecalis plasmids pAD1 and pAD2 and studies relating to transposition of Tn917. J Bacteriol 152: 1220-1230.
6. Cociancich, S., Ghazi, A., Hetru, C., Hoffmann, J.A., and Letellier, L. (1993) Insect defensin, an inducible antibacterial peptide, that forms voltage-dependent channels in Micrococcus luteus. J Biol Chem 268: 19239-19245.
7. 2, and structural revisions of duramycin and cinnamycin. In Nisin and Novel Lantibiotics. Jung, G., and Sahl, H.G. (eds). Leiden: ESCOM, pp. 131-140.
8. Giebler, S., Gotz, F., and Kupke, T. (1996) Serine protease EpiP from Staphylococcus epidermidis catalyzes the processing of the epidermin precursor peptide. J Bacteriol 178: 584-288.
9. Gilmore, M.S., Segarra, R.A., and Booth, M.C. (1990) An HlyB-type function is required for expression of the Enterococcus faecalis hemolysin/bacteriocin. Infect Immun 58: 3914-3923.
10. Gilmore, M.S., Segarra, R.A., Booth, M.C., Bogie, C.P., Hall, L.R., and Clewell, D.B. (1994) Genetic structure of the Enterococcus faecalis plasmid pAD1 encoded cytolytic toxin system and its relationship to lantibiotic determinants. J Bacteriol 176: 7335-7344.
11. Granato, P.A., and Jackson, R.W. (1971a) Characterization of the A component of Streptococcus zymogenes lysin. J Bacteriol 107: 551-556.
12. Granato, P.A., and Jackson, R.W. (1971b) Purification and characterization of the L component of Streptococcus zymogenes lysin. J Bacteriol 108: 804-808.
13. Greer, J. (1990) Comparative modelling methods: application to the family of mammalian serine proteases. Proteins 7: 317-334.
14. Greer, J. (1991) Comparative modelling of homologous proteins. Meth Enzymol 202: 239-525.
15. Gros, P., Betzel, C., Dauter, Z., Wilson, K.S., and Hol, W.G.J. (1989) Molecular dynamic refinement of a thermitase-eglinc complex at 1.98 Å resolution and a comparison of two crystal forms that differ in calcium content. J Mol Biol 210: 347-367.
16. Gross, E., and Morrell, J. (1971) The structure of nisin. J Am Chem Soc 93: 4634-4635.
17. Harlow, E., and Lane, D. (1988) Antibodies. A Laboratory Manual. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press.
18. Havarstein, L.S., Diep, D.B., and Nes, I.F. (1995) A family of bacteriocin ABC transporters carry out proteolytic processing of their substrate concomitant with export. Mol Microbiol 16: 229-240.
19. Heinz, D.W., Priestle, J.P., Raheul, J., Wilson, K.S., and Grutter, M.G. (1991) Refined crystal structure of subtilisin novo in complex with wildtype and two mutant eglins. Comparison with other serine protease inhibitor complexes. J Mol Biol 217: 353-371.
20. Heukeshoven, J., and Dernick, R. (1988) Simplified method for silver staining of proteins in polyacrylamide gels and the mechanism of staining. Electrophoresis 6: 103-112.
21. Hewick, P.M., Hunkapiller, M.W., Hood, L.E., and Dreyer, W.J. (1981) A gas liquid solid phase peptide and protein sequenator. J Biol Chem 256: 7990-7997.
22. Huycke, M.M., and Gilmore, M.S. (1995) Frequency of aggregation substance and cytolysin genes among enterococcal endocarditis isolates. Plasmid 34: 152-156.
23. Huycke, M.M., Speigel, C.A., and Gilmore, M.S. (1991) Bacteremia caused by haemolytic high-level gentamicin resistant Enterococcus faecalis. Antimicrob Ag Chemother 35: 1626-1634.
24. Ike, Y., Hashimoto, H., and Clewell, D.B. (1987) High incidence of hemolysin production by Enterococcus (Streptococcus) faecalis strains associated with human parenteral infections. J Clin Microbiol 25: 1524-1528.
25. Ike, Y., Clewell, D.B., Segarra, R.A., and Gilmore, M.S. (1990) Genetic analysis of the pAD1 hemolysin/bacteriocin determinant in Enterococcus faecalis: Tn917 insertional mutagenesis and cloning. J Bacteriol 172: 155-163.
26. Jett, B.D., Jensen, H.G., Nordquist, R.E., and Gilmore, M.S. (1992) Contribution of the pAD1-encoded cytolysin to the severity of experimental Enterococcus faecalis endophthalmitis. Infect Immun 60: 2445-2452.
27. Jett, D.D., Huycke, M.M., and Gilmore, M.S. (1994) Virulence of enterococci. Clin Microbiol Rev 7: 462-478.
28. Jung, G. (1991) Lantibiotics: a survey. In Nisin and Novel Lantibiotics. Jung, G., and Sahl, H.G. (eds). Leiden: ESCOM, pp. 1-34.
29. Kuipers, O.P., Rollema, H.S., Yap, Y.M.G.J., Boot, H.J., and Siezen, R.J. (1992) Engineering dehydrated residues in the antimicrobial peptide nisin. J Biol Chem 267: 24340-23446.
30. Kuipers, O.P., Beerthuyzen, M.M., Siezen, R.J., and De Vos, W.M. (1993) Characterization of the nisin gene cluster nisABTCIPR of Lactococcus lactis. Eur J Biochem 216: 281-291.
31. Liu, W., and Hansen, J.N. (1992) Enhancement of the chemical and antimicrobial properties of subtilin by site directed mutagenesis. J Biol Chem 267: 25078-25083.
32. van der Meer, J.R., Polman, J., Beerthuyzen, M.M., Siezen, R.J., Kuipers, O.P., and De Vos, W.M. (1993) Characterization of the Lactobacillus lactis nisin A operon genes nisP, encoding a subtilisin-like serine protease involved in precursor processing, and nisR, encoding a regulatory protein involved in nisin biosynthesis. J Bacteriol 175: 2578-2588.
33. Murray, B.E. (1990) The life and times of the enterococcus. Clin Microbiol Rev 3: 46-65.
34. Ring, C.S., and Cohen, F.E. (1993) Modeling protein structures: construction and their application. FASEB J 7: 783-789.
35. Sahl, H.G., Grossgarten, M., Widger, W.R., Cramer, W.A., and Brandis, H. (1985) Structural similarities of the staphylococcin-like peptide Pep-5 to the peptide nisin. Antimicrob Ag Chemother 27: 836-840.
36. Sahl, H.G., Jack, R.W., and Bierbaum, G. (1995) Biosynthesis and biological activities of lantibiotics with unique post-translational modifications. Eur J Biochem 230: 827-853.
37. Sambrook, J., Fritsch, E.F., and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual. 2nd edn. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press.
38. Schnell, N., Engelke, G., Augustin, J., Rosenstein, R., Ungermann, V., Gotz, F., and Entian, K.D. (1992) Analysis of genes involved in the biosynthesis of lantibiotic epidermin. Eur J Biochem 204: 57-68.
39. Segarra, R.A., Booth, M.C., Morales, D.A., Huycke, M.M., and Gilmore, M.S. (1991) Molecular characterization of the Enterococcus faecalis cytolysin activator. Infect Immun 59: 1239-1246.
40. Siezen, R.J., De Vos, W.M., Leunissen, J.A.M., and Dijkstra, B.W. (1991) Homology modelling and protein engineering strategy of subtilases, the family of subtilisin like serine proteinases. Prot Eng 4: 719-737.
41. Siezen, R.J., Rollema, H.S., Kuipers, O.P., and de Vos, W.M. (1995) Homology modelling of the Lactococcus lactis leader peptidase NisP and its interaction with the precursor of the lantibiotic nisin. Prot Eng 8: 117-125.
42. Studier, F.W., Rosenberg, A.H., Dunn, J.J., and Dubendorff, J.W. (1990) Use of T7 RNA polymerase to direct expression of cloned genes. Meth Enzymol 185: 60-89.
43. Sutcliffe, M.J., Haneef, I., Carney, D., and Blundell, T.L. (1987) Knowledge based modelling of homologous proteins, Part I: Three dimensional frameworks derived from the simultaneous superposition of multiple structures. Prot Eng 1: 377-384.
44. Urritia, R., Cruciani, R.A., Barker, J.L, and Kacher, B. (1989) Spontaneous polymerization of the antibiotic peptide magainin 2. FEBS Lett 247: 17-21.
45. Wandersman, C. (1989) Secretion, processing and activation of bacterial extracellular proteases. Mol Microbiol 3: 1825-1831.