Sequence of the Corynebacterium glutamicum pyruvate carboxylase gene

Applied Microbiology and Biotechnology

Volumn 50, Issue 3, 1998, Pages 346-352

  Koffas, M A G ^a   Ramamoorthi, R ^a   Pine, W A ^a   Sinskey, A J ^a   Stephanopoulos, G ^a  

^a Massachusetts Institute of Technology Cambridge   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

PYRUVATE DECARBOXYLASE;

ARTICLE; BACTERIAL GENE; CORYNEBACTERIUM GLUTAMICUM; GENE SEQUENCE; MYCOBACTERIUM TUBERCULOSIS; NONHUMAN; PROTEIN EXPRESSION; SEQUENCE HOMOLOGY;

AMINO ACID SEQUENCE; BLOTTING, WESTERN; CLONING, MOLECULAR; CORYNEBACTERIUM; COSMIDS; DNA, BACTERIAL; GENE LIBRARY; HUMANS; IMMUNOBLOTTING; MOLECULAR SEQUENCE DATA; POLYMERASE CHAIN REACTION; PYRUVATE CARBOXYLASE; SEQUENCE ANALYSIS, DNA; SEQUENCE HOMOLOGY, AMINO ACID;

ACTINOBACTERIA (CLASS); BACTERIA (MICROORGANISMS); CORYNEBACTERIUM GLUTAMICUM; MYCOBACTERIUM; MYCOBACTERIUM TUBERCULOSIS; UNCULTURED ACTINOMYCETE; UNIDENTIFIED BACTERIOPHAGE;

EID: 0031719437     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s002530051302     Document Type: Article

References (33)

1. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990) Basic local alignment search tool. J Mol Biol 215: 403-410
2. Attwood PV (1995) The structure and the mechanism of action of pyruvate carboxylase. Int J Biochem Cell Biol 27: 231-249
3. Brewster NK, Val DL, Walker ME, Wallace JC (1994) Regulation of pyruvate carboxylase isozyme (PYC1, PYC2) gene expression in Saccharomyces cerevisiae during fermentative and nonfermentative growth. Arch Biochem Biophys 311: 62-71
4. Charles AM, Willer DW (1984) Pyruvate carboxylase from Thiobacillus novellus: properties and possible functions. Can J Microbiol 30: 532-539
5. Dunn MF, Encarnacion S, Araiza G, Vargas C, Davalos A, Humberto P, Mora Y, Mora J (1996) Pyruvate carboxylase from Rhizobium etli: mutant characterization, nucleotide sequence and physiological role. J Bacteriol 178: 5960-5970
6. Fry DC, Kuby SA, Mildvan AS (1986) ATP-binding site of adenylate kinase: mechanistic implications of its homology with ras-encoded p21. FI-ATPase, and other nucleotide-binding proteins. Proc Natl Acad Sci USA 83: 907-911
7. Gubler M, Park SM, Jetten M, Stephanopoulos G, Sinskey AJ (1994) Effects of phosphoenolpyruvate carboxylase deficiency on metabolism and lysine production in Corynebacterium glutamicum. Appl Microbiol Biotechnol 40: 857-863
8. Hanahan D (1983) Studies on transformation of Escherichia coli with plasmids. J Mol Biol 166: 557-580
9. Jager W, Peters-Wendisch PG, Kalinowski J, Puhler A (1996) A Corynebacterium glutamicum gene encoding a two domain protein similar to biotin carboxylases and biotin-carboxyl-carrier proteins. Arch Microbiol 166: 76-82
10. Jetten MSM, Sinskey AJ (1993) Characterization of phosphoenolpyruvate carboxykinase from Corynebacterium glutamicum. FEMS Microbiol Lett 111: 183-188
11. Keilhauer C, Eggeling L, Sahm H (1993) Isoleucine synthesis in Corynebacterium glutamicum: molecular analysis of the ilvB-ilvN-ilvC operon. J Bacteriol 175: 5595-5603
12. Kondo H, Kazata Y, Saito A, Fuji K (1997) Cloning and nucleotide sequence of Bacillus stearothermophilus pyruvate carboxylase. Gene 191: 47-50
13. Kumer GK, Haase FC, Phillips NFB, Wood HG (1988) Involvement and identification of a tryptophanyl residue at the pyruvate-binding site of transcarboxylase. Biochemistry 27: 5978-5983
14. Lim F, Morris CP, Occhiodoro F, Wallace JC (1988) Sequence and domain structure of yeast pyruvate carboxylase J Biol Chem 263: 11493-11497
15. Milrad de Forchetti SR, Cazullo JJ (1976) Some properties of the pyruvate carboxylase from Pseudomonas fluorescens. J Gen Microbiol 93: 75-81
16. O'Brien RW, Chuang DT, Taylor BL, Utter MF (1977) Novel enzymic machinery for the metabolism of oxaloacetate, phosphoenolpyruvate and pyruvate in Pseudomonas citronellolis. J Biol Chem 252: 1257-1263
17. Park SM, Sinskey AJ, Stephanopoulos G (1997a) Metabolic and physiological studies of Corynebacterium glutamicum mutants. Biotechnol Bioeng 55: 864-879
18. 13C-NMR spectroscopy and GC-MS. Appl Microbiol Biotechnol 47: 430-140
19. Peters-Wendisch PG, Eikmanns BJ, Thierbach G, Bachmann B, Sahm H (1993) Phosphoenolpyruvate carboxylase in Corynebacterium glutamicum is dispensable for growth and lysine production FEMS Microbiol Lett 112: 269-274
20. Peters-Wendisch PG, Wendisch VF, Graaf AA de, Eikmanns BJ, Sahm H (1996) C3-Carboxylation as an anaplerotic reaction in phopshoenolpyruvate carboxylase-deficient Corynebacterium glutamicum. Arch Microbiol 165: 387-396
21. Peters-Wendisch PG, Wendisch VF, Paul S, Eikmanns BJ, Sahm H (1997) Pyruvate carboxylase as an anaplerotic enzyme in Corynebacterium glutamicum. Microbiology 143: 1095-1103
22. Peters-Wendisch PG, Kreutzer C, Kalinowski J, Patek M, Sahm H, Eikmanns BJ (1998) Pyruvate carboxylase from Corynebacterium glutamicum: characterisation, expression and inactivation of the pyc gene. Microbiology (in press)
23. Post LE, Post DJ, Raushel FM (1990) Dissection of the functional domains of Escherichia coli carbamoyl phosphate synthetase by site-directed mutagenesis. J Biol Chem 265: 7742-7747
24. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
25. Scrutton MC (1978) Fine control of the conversion of pyruvate (phosphoenolpyruvate) to oxaloacetate in various species. FEBS Lett 89: 1-9
26. Scrutton MC, Taylor BL (1974) Isolation and characterization of pyruvate earboxylase from Azotobacter vinelandii OP. Arch Biochem Biophys 164: 641-654
27. Stryer L (1988) Biochemistry, 3rd edn. Freeman, NY
28. Toh H, Kawamura T, Izui K (1994) Molecular evolution of phosphoenolpyruvate carboxylase. Plant Cell Environ 17: 31-43
29. Tomioka N, Shinozaki K, Sugiura M (1981) Molecular cloning and characterization of ribosomal RNA genes from a blue green alga, Anacystis nidulans Mol Gen Genet 184: 359-363
30. Tosuka O, Morioka H, Takinami K (1979) The role of biotin-dependent pyruvate earboxylase in L-lysine production. Agric Biol Chem 43: 1513-1519
31. Towbin H, Staehelin T, Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76: 4350-4354
32. Vallino JJ, Stephanopoulos G (1993) Metabolic flux distributions in Corynebacterium glutamicum during growth and lysine overproduction. Biotechnol Bioeng 41: 633-646
33. Wexler ID, Du Y, Lisgaris MV, Mandal SK, Freytag SO, Yang B, Liu T, Kwon M, Patel MS, Kerr DS (1994) Primary amino acid sequence and structure of human pyruvate earboxylase. Biochim Biophys Acta 1227: 46-52