Identification of V(D)J recombination coding end intermediates in normal thymocytes

Journal of Molecular Biology

Volumn 267, Issue 1, 1997, Pages 1-9

  Livák, Ferenc ^b   Schatz, David G ^a  

^a HOWARD HUGHES MEDICAL INSTITUTE   (United States)

^b NONE

Author keywords

Coding end; DNA repair; Recombination activating gene; T cell receptor chain; V(D)J recombination

Indexed keywords

LYMPHOCYTE ANTIGEN RECEPTOR; T LYMPHOCYTE RECEPTOR; T LYMPHOCYTE RECEPTOR ALPHA CHAIN;

ANIMAL CELL; ARTICLE; CHEMICAL STRUCTURE; CONTROLLED STUDY; DNA REPAIR; DNA SEQUENCE; DNA STRAND BREAKAGE; GENE REARRANGEMENT; GENETIC RECOMBINATION; LYMPHOID CELL; MOUSE; NONHUMAN; PRIORITY JOURNAL; STEM CELL; T LYMPHOCYTE RECEPTOR GENE; THYMOCYTE;

ANIMALIA; MURINAE; VERTEBRATA;

EID: 0031582059     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.1996.0834     Document Type: Article

References (37)

1. Bogue M. A., Zhu C. M., Aguilarcordova E., Donehower L. A., Roth D. B. p53 is required for both radiation-induced differentiation and rescue of V(D)J rearrangement in scid mouse thymocytes. Genes Dev. 10:1996;553-565.
2. Danska J. S., Pflumio F., Williams C. J., Huner O., Dick J. E., Guidos C. J. Rescue of T cell-specific V(D)J recombination in scid mice by DNA-damaging agents. Science. 266:1994;450-455.
3. Eastman Q. M., Leu T. M. J., Schatz D. G. Initiation of V(D)J recombination in vitro obeying the 12/23 rule. Nature. 380:1996;85-88.
4. Fishman-Lobell J., Haber J. E. Removal of nonhomologous DNA ends in double strand break recombination: the role of the yeast ultraviolet repair gene RAD1. Science. 258:1992;480-484.
5. Fishman-Lobel J., Rudin N., Haber J. E. Two alternate pathways of double strand break repair that are kinetically separable and independently modulated. Mol. Cell. Biol. 12:1992;1292-1303.
6. Gerstein R. M., Lieber M. R. Extent to which homology can constrain coding exon junctional diversity in V(D)J recombination. Nature. 363:1993;625-627.
7. Gottlieb T. M., Jackson S. P. The DNA-dependent protein kinase: requirement for DNA ends and association with Ku antigen. Cell. 72:1993;131-142.
8. Hesse J. E., Lieber M. R., Mizuuchi K., Gellert M. V(D)J recombination: a functional definition of the joining signals. Genes Dev. 3:1989;1053-1061.
9. Itohara S., Mombaerts P., Lafaille J., Iacomini J., Nelson A., Clarke A. R., Hooper M. L., Farr A., Tonegawa S. T cell receptor δ gene mutant mice: independent generation of αβ T cells and programmed rearrangements of γδ TCR genes. Cell. 72:1993;337-348.
10. Jackson S. P., Jeggo P. A. DNA double-strand break repair and V(D)J recombination: involvement of DNA-PK. Trends Biochem. Sci. 20:1995;412-415.
11. Kabotyanski E. B., Zhu C. M., Kallick D. A., Roth D. B. Hairpin opening by single-strand-specific nucleases. Nucl. Acids Res. 23:1995;3872-3881.
12. Koop B. F., Wilson R. K., Wang K., Vernooij B., Zallwer D., Kuo C., Seto D., Toda M., Hood L. Organization, structure, and function of 95 kb of DNA spanning the murine T-cell receptor C alpha/C delta region. Genomics. 13:1992;1209-1230.
13. Kornberg A., Baker T. A. DNA Replication. 1992;W. H. Freeman and Company, New York.
14. Landau N. R., Schatz D. G., Rosa M., Baltimore D. Increased frequency of N-regional insertion in a murine pre-B-cell line infected with a terminal deoxynucleotidyl transferase retroviral expression vector. Mol. Cell. Biol. 7:1987;3237-3243.
15. Lieber M. R. Site-specific recombination in the immune system. FASEB J. 5:1991;2934-2944.
16. Lieber M. R., Hesse J. E., Mizuuchi K., Gellert M. Lymphoid V(D)J recombination: nucleotide insertion at signal joints as well as coding joints. Proc. Natl Acad. Sci. USA. 85:1988;8588-8592.
17. Livak F., Schatz D. G. T-cell receptor alpha locus V(D)J recombination by-products are abundant in thymocytes and mature T cells. Mol. Cell. Biol. 16:1996;609-618.
18. Livak F., Welsh S. C., Guidos C. J., Crispe I. N., Danska J. S., Schatz D. G. Transient restoration of gene rearrangement at multiple T cell receptor loci in γ irradiated scid mice. J. Exp. Med. 184:1996;419-428.
19. Lydall D., Weinert T. Yeast checkpoint genes in DNA damage processing: implications for repair and arrest. Science. 270:1995;1488-1492.
20. Mallick C. A., Dudley E. C., Viney J. L., Owen M. J., Hayday A. C. Rearrangement and diversity of T cell receptor beta chain genes in thymocytes: a critical role for the beta chain in development. Cell. 73:1993;513-519.
21. McBlane J. F., van Gent D. C., Ramsden D. A., Romeo C., Cuomo C. A., Gellert M., Oettinger M. A. Cleavage at a V(D)J recombination signal requires only RAG1 and RAG2 proteins and occurs in two steps. Cell. 83:1995;387-395.
22. Nadel B., Tehranchi S., Feeney A. J. Coding end processing is similar throughout ontogeny. J. Immunol. 154:1995;6430-6436.
23. Oettinger M. A., Schatz D. G., Gorka C., Baltimore D. RAG-1 and RAG-2, adjacent genes that synergistically activate V(D)J recombination. Science. 248:1990;1517-1523.
24. Ramsden D. A., Gellert M. Formation and resolution of double-strand break intermediates in V(D)J rearrangement. Genes Dev. 9:1995;2409-2420.
25. Resnick M. A. The repair of double strand breaks in DNA: a model involving recombination. J. Theoret. Biol. 59:1976;97-106.
26. Roth D. B., Menetski J. P., Nakajima P. B., Bosma M. J., Gellert M. V(D)J recombination: broken DNA molecules with covalently sealed (hairpin) coding ends in scid mouse thymocytes. Cell. 70:1992a;983-991.
27. Roth D. B., Nakajima P. B., Menetski J. P., Bosma M. J., Gellert M. V(D)J recombination in mouse thymocytes - double-stranded breaks near T-cell receptor delta rearrangement signals. Cell. 69:1992b;41-53.
28. Roth D. B., Zhu C. M., Gellert M. Characterization of broken DNA molecules associated with V(D)J recombination. Proc. Natl Acad. Sci. USA. 90:1993;10788-10792.
29. Schatz D. G., Oettinger M. A., Baltimore D. The V(D)J recombination activating gene (RAG-1). Cell. 59:1989;1035-1048.
30. Schlissel M., Constantinescu A., Morrow T., Baxter M., Peng A. Double-strand signal sequence breaks in V(D)J recombination are blunt, 5′-phosphorylated, RAG-dependent, and cell cycle regulated. Genes Dev. 7:1993;2520-2532.
31. Stanhope-Baker P., Hudson K. M., Shaffer A. L., Constantinescu A., Schlissel M. S. Cell type specific chromatin structure determines the targeting of V(D)J recombinase activity in vitro. Cell. 85:1996;887-897.
32. Tomkinson A. E., Bardwell A. J., Bardwell L., Tappe N. J., Friedberg E. C. Yeast DNA repair and recombination proteins Rad1 and Rad10 constitute a single stranded DNA endonuclease. Nature. 362:1993;860-862.
33. Tonegawa S. Somatic generation of antibody diversity. Nature. 302:1983;575-581.
34. van Gent D. C., McBlane J. F., Ramsden D. A., Sadofsky M. J., Hesse J. E., Gellert M. Initiation of V(D)J recombination in a cell-free system. Cell. 81:1995;925-934.
35. Weaver D. T. What to do at an end-DNA double-strand-break repair. Trends Genet. 11:1995;388-392.
36. Zhu C., Roth D. B. Characterization of coding ends in thymocytes of scid mice: implications for the mechanism of V(D)J recombination. Immunity. 2:1995;101-112.
37. Zhu C., Bogue M. A., Lim D., Hasty P., Roth D. B. Ku86 deficient mice exhibit severe combined immunodeficiency and defective processing of V(D)J recombination intermediates. Cell. 86:1996;379-389.