Damage and replication checkpoint control in fission yeast is ensured by interactions of Crb2, a protein with BRCT motif, with Cut5 and Chk1

Genes and Development

Volumn 11, Issue 24, 1997, Pages 3387-3400

  Saka, Yasushi ^a,b   Esashi, Fumiko ^a   Matsusaka, Takahiro ^a   Mochida, Satoru ^a   Yanagida, Mitsuhiro ^a  

^a KYOTO UNIVERSITY   (Japan)

^b NATIONAL INSTITUTE FOR MEDICAL RESEARCH   (United Kingdom)

Author keywords

DNA polymerase; Hydroxyurea; Phosphorylation; Two hybrid screen; UV damage

Indexed keywords

DNA; DNA POLYMERASE;

ARTICLE; CELL CYCLE; DNA DAMAGE; DNA REPLICATION; ENZYME PHOSPHORYLATION; FUNGAL GENETICS; GENOME; NONHUMAN; PRIORITY JOURNAL; SCHIZOSACCHAROMYCES POMBE; ULTRAVIOLET IRRADIATION; YEAST;

SCHIZOSACCHAROMYCES POMBE;

EID: 0031438163     PISSN: 08909369     EISSN: None     Source Type: Journal    
DOI: 10.1101/gad.11.24.3387     Document Type: Article

References (66)

1. 2 checkpoint pathways in Schizosaccharomyces pombe. EMBO J. 11: 1343-1350.
2. Al-Khodairy, F., E. Fotou, K.S. Sheldrick, D.J. Griffiths, A.R. Lehmann, and A.M. Carr. 1994. Identification and characterization of new elements involved in checkpoint and feedback controls in fission yeast. Mol Biol. Cell. 5: 147-160.
3. Araki, H., S.H. Leem, A. Phongdara, and A. Sugino. 1995. Dpb11, which interacts with DNA polymerase II epsilon in Saccharomyces cerevisiae, has a dual role in S-phase progression at a cell cycle checkpoint. Proc. Natl. Acad. Sci. 92: 11791-11795.
4. Bentley, N., D. Holtzman, G. Flaggs, K. Keegan, A. DeMaggio, J. Ford, M. Hoekstra, and A. Carr. 1996. The Schizosaccharomyces pombe rad3 checkpoint gene. EMBO J. 15: 6641-6651.
5. Bork, P., K. Hoffmann, P. Bucher, A.F. Neuwald, S.F. Altschul, and E.V. Koonin. 1997. A superfamily of conserved domains in DNA damage responsive cell cycle checkpoint proteins. FASEB J. 11: 68-76.
6. Callaebaut, I. and J.P. Mornon. 1997. From BRCA1 to RAP1: A widespread BRCT module closely associated with DNA repair. FEBS Lett. 400: 25-30.
7. 2. Curr. Genet. 11: 119-125.
8. Duck, P., A. Nasim, and A.P. James. 1976. Temperature-sensitive mutant of Schizosaccharomyces pombe exhibiting enhanced radiation sensitivity. J. Bact. 128: 536-539.
9. El-Deiry, W.S., T. Tokino, V.E. Velculescu, D.B. Levy, R. Parsons, J.M. Trent, D. Lin, W.E. Mercer, K.W. Kinzler, and B. Vogelstein. 1993. WAF1, a potential mediator of p53 tumor suppression. Cell 75: 817-825.
10. Elledge, S.J. 1996. Cell cycle checkpoints: Preventing an identity crisis. Science 274: 1664-1672.
11. Enoch, T., T. Carr, and P. Nurse. 1992. Fission yeast genes involved in coupling mitosis to completion of DNA replication. Genes & Dev. 6: 2035-2046.
12. Fenech, M., A.M. Carr, J. Murray, F.Z. Watts, and A.R. Lehmann. 1991. Cloning and characterization of the rad4 gene of Schizosaccharomyces pombe; a gene showing short regions of sequence similarity to the human XRCC1 gene. Nucleic Acids Res. 19: 6737-6741.
13. + of the fission yeast Schizosaccharomyces pombe encode the large and small subunits of ribonucleotide reductase. Mol. & Gen. Genet. 238: 241-251.
14. Fields, S. and O. Song. 1989. A novel genetic system to detect protein-protein interactions. Nature 340: 245-246.
15. Ford, J.C., F. Al-Khodairy, E. Fotou, K.S. Sheldrick, D.J. Griffiths, and A.M. Carr. 1994. 14-3-3 protein homologs required for the DNA damage checkpoint in fission yeast. Science 265: 533-535.
16. Francesconi, S., A.M. De Recondo, and G. Baldacchi. 1995. DNA polymerase delta is required for the replication feedback control of cell cycle progression in Schizosaccharomyces pombe. Mol. Gen. Genet. 246: 561-569.
17. chk1 protein kinase is required for the DNA replication checkpoint at 37°C in fission yeast. EMBO J. 16: 1332-1341.
18. Gordon, C.B. and P. Fantes. 1986. The cdc22 gene of Schizosaccharomyces pombe encodes a cell cycle regulated transcript. EMBO J. 5:2981-2985.
19. Griffiths, D.J., N.C. Barbet, S. McCready, A.R. Lehmann, and A.M. Carr. 1995. Fission yeast rad17: A homologue of budding yeast RAD24 that shares regions of sequence similarity with DNA polymerase accessory proteins. EMBO J. 14: 5812-5823.
20. Guan, K. and J.E. Dixon. 1991. Eucaryotic proteins expressed in Escherichia coli: An improved thrombin cleavage and purification procedure of fusion proteins with glutathion S-transferase. Analytical Biochem. 192: 262-267.
21. Gutz, H., H. Heslot, U. Leupold, and N. Lopreno. 1974. Schizosaccharomyces pombe. In Handbook of genetics 1 (ed. R.C. King), pp. 395-446. Plenum Press, New York, NY.
22. Hartwell, L.H. and T.A. Weinert. 1989. Checkpoint: Controls that ensure the order of cell cycle events. Science 246: 629-634.
23. cdc2-mitotic B cyclin complex. Cell 78: 813-822.
24. Heim, R., A.B. Cubitt, and R.Y. Tsien. 1995. Improved green fluorescence. Nature 373: 663-664.
25. Hirano, T., S. Funahashi, T. Uemura, and M. Yanagida. 1986. Isolation and characterization of Schizosaccharomyces pombe cut mutants that block nuclear division but not cytokinesis. EMBO J. 5: 2973-2979.
26. Iwabuchi, K., P.L. Bartel, B. Li, R. Marraccino, and S. Fields. 1994. Two cellular proteins that bind to wild-type but not mutant p53. Proc. Natl. Acad. Sci. 91: 6098-6102.
27. + gene product couples S phase to start and mitosis. Cell 74: 371-382.
28. Kitazuno, A. and T. Matsumoto. 1997. Isogaba maware, Quality control of gene DNA by checkpoints. BioEssays (in press).
29. Koonin, E.V., S.F. Altschul, and P. Bork. 1996. Functional motifs. Nature Genet. 13: 266-268.
30. Kostrub, C., F. Al-Khodairy, H. Ghazizadeh, A. Carr, and T. Enoch. 1997. Molecular analysis of hus1+, a fission yeast gene required for S-M and DNA damage checkpoints. Mol. Gen. Genet. 254: 389-399.
31. Kubota, Y., R.A. Nash, A. Klugland, P. Schär, D.B. Barnes, and T. Lindahl. 1996. Reconstitution of DNA base excision-repair with purified human proteins: Interaction between DNA polymerase β and the XRCC1 protein. EMBO J. 15: 6662-6670.
32. Larimer, F.W., J.R. Perry, and A.A. Hardigree. 1989. The REV1 gene of Saccharomyces cerevisiae: Isolation, sequence, and functional analysis. J. Bacteriol. 171: 230-237.
33. Lieberman, H.B., K.M. Hopkins, M. Nass, D. Demetrick, and S. Davey. 1996. A human homologue of the Schizosaccharomyces pombe rad9+ checkpoint gene. Proc. Natl. Acad. Sci. 93: 13890-13895.
34. Lydall, D. and T. Weinert. 1995. Yeast checkpoint genes in DNA damage processing: Implications for repair and arrest. Genes & Dev. 270: 1488-1491.
35. Maundrell, K. 1990. nmt1 of fission yeast. J. Biol. Chem. 265: 10857-10864.
36. Miki, T., C.L. Smith, J.E. Long, A. Eva, and T.P. Fleming. 1993. Oncogene ect2 is related to regulators of small GTP-binding protein. Nature 362: 462-465.
37. Mitchison, J.M. 1970. Physiological and cytological methods for Schizosaccharomyces pombe. Methods Cell Physiol. 4: 131-165.
38. Mizukami, T., W.I. Chang, I. Gargavitsev, N. Kaplan, D. Lombardi, T. Matsumoto, O. Niwa, A. Kounousu, M. Yanagida, T.G. Marr, and D. Beach. 1993. A 13 kb resolution cosmid map of the 14 Mb fission yeast genome by nonrandom sequence-tagged site mapping. Cell 73: 121-132.
39. + gene. Nature 367: 236-242.
40. Murakami, H. and H. Okayama. 1995. A kinase from fission yeast responsible for blocking mitosis in S-phase. Nature 374: 817-819.
41. dis1 required for sister chromatid separation is a novel microtubule and spindle pole body associating protein phosphorylated at the Cdc2 target sites. Genes & Dev. 9: 1572-1585.
42. Nasmyth, K. 1996. Viewpoint: Putting the cell cycle in order. Science 274: 1643-1645.
43. Navas, T.A., Y. Sanchez, and S.J. Elledge. 1996. RAD9 and DNA polymerase ∈ form parallel sensory branches for transducing the DNA damage checkpoint signal in Saccharomyces cerevisiae. Genes & Dev. 10: 2632-2643.
44. Neer, E.J., C.J. Schmidt, R. Nambudripad, and T.F. Smith. 1994. The ancient regulatory-protein family of WD-repeat proteins. Nature 371: 297-300.
45. cdc18 plays a major role controlling the initiation of DNA replication in fission yeast. Cell 83: 397-405.
46. Nugent, C.I., T.R. Hughes, N.F. Lue, and V. Lundblad. 1996. Cdc13p: A single-strand telomeric DNA-binding protein with a dual role in yeast telomere maintenance. Science 274: 249-251.
47. Nurse, P., P. Thuriaux, and K. Nasmyth. 1976. Genetic control of the cell division cycle in the fission yeast Schizosaccharomyces pombe. Mol. Gen. Genet. 146: 167-178.
48. Paulovich, A.G. and L.H. Hartwell. 1995. A checkpoint regulates the rate of progression through S phase in S. cerevisiae in response to DNA damage. Cell 82: 841-847.
49. O'Connell, M.J., J.M. Raleigh, H.M. Verkade, and P. Nurse. 1997. Chk1 is a wee1 kinase in the G2 DNA damage checkpoint inhibiting cdc2 by Y15 phosphorylation. EMBO J. 16: 545-554.
50. Rhind, N., B. Furnari, and P. Russell. 1997. Cdc2 tyrosine phosphorylation is required for the DNA damage checkpoint in fission yeast. Genes & Dev. 11: 504-511.
51. Rothstein, R.J. 1983. One-step gene disruption in yeast. Methods Enzymol. 101: 202-211.
52. Rowley, R., S. Subramani, and P.G. Young. 1992. Checkpoint controls in Schizosaccharomyces pombe: rad1. EMBO J. 11: 1335-1342.
53. +. Cell 74: 383-393.
54. Saka, Y., P. Fantes, T. Sutani, C. McInerny, J. Creanor, and M. Yanagida. 1994a. Fission yeast cut5 links nuclear chromatin and M phase regulator in the replication checkpoint control. EMBO J. 13: 5319-5329.
55. Saka, Y., P. Fantes, and M. Yanagida. 1994b. Coupling of DNA replication and mitosis by fission yeast rad4/cut5. J. Cell Sci. (suppl.) 18: 57-61.
56. Samejima, I., T. Matsumoto, Y. Nakaseko, D. Beach, and M. Yanagida. 1993. Identification of seven new cut genes involved in Schizosaccharomyces pombe mitosis. J. Cell Sci. 105: 135-143.
57. 2 checkpoints: Fission yeast as a model system. BioEssays 15: 775-782.
58. 1 arrest defines a second checkpoint for damaged DNA in the cell cycle of Saccharomyces cerevisiae. Proc. Natl. Acad. Sci. 90: 7985-7989.
59. Singh, J. and A.J. Klar. 1993. DNA polymerase-α is essential for mating-type switching in fission yeast. Nature 361: 271-273.
60. Thompson, L.H., K.W. Brookman, J.J. Jones, S.A. Allen, and A.V. Carrano. 1990. Molecular cloning of the human XRCC1 gene, which corrects defective DNA strands repair and sister chromatid exchange. Mol. Cell. Biol. 10: 6160-6171.
61. Walworth, N.C. and R. Bernards. 1996. rad-Dependent response of the chk1-encoded protein kinase at the damage checkpoint. Science 271: 353-356.
62. Walworth, N., S. Davey, and D. Beach. 1993. Fission yeast chk1 protein kinase links rad checkpoint pathway to cdc2. Nature 363: 368-371.
63. Weinert, T.A. and L.H. Hartwell. 1988. The RAD9 gene controls the cell cycle response to DNA damage in Saccharomyces cerevisiae. Science 241: 317-322.
64. Wilson, J., S. Wilson, N. Warr, and F.Z. Watts. 1997. Isolation and characterization of the Schizosaccharomyces pombe rhp9 gene: A gene required for the DNA damage checkpoint but not the replication checkpoint. Nucleic Acids Res. 25: 2138-2145.
65. Yamada, H., K. Kumada, and M. Yanagida. 1997. Distinct subunit functions and cell cycle regulated phosphorylation of 20S APC/Cyclosome required for anaphase in fission yeast. J. Cell Sci. 110: 1793-1804.
66. Yamashita, Y.M., Y. Nakaseko, I. Samejima, K. Kazuki, H. Yamada, D. Michaelson, and M. Yanagida. 1996. 20S cyclosome complex formation and proteolytic activity inhibited by the cAMP/PKA pathway. Nature 384: 276-279.