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Volumn 7, Issue 1, 1997, Pages 76-85

Lac repressor-operator complex

Author keywords

[No Author keywords available]

Indexed keywords

ISOPROPYL THIOGALACTOSIDE; PROTEIN;

EID: 0031048509     PISSN: 0959440X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0959-440X(97)80010-3     Document Type: Article
Times cited : (28)

References (97)
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    • sym operators to a Lacl tetramer: evidence for contributions of non-operator DNA binding by wrapping and looping. of special interest J Mol Biol. 260:1996;697-717 The authors thoroughly examine lac repressor¥mmetric operator interactions over a range of protein, DNA and salt concentrations. The repressor tetramer was found to be more stable than previously thought. At high salt concentrations, binding of the repressor to symmetric operator sites on a plasmid is noncooperative. At lower salt concentrations, anticooperativity of binding a second operator site increases. The authors propose that nonspecific binding and DNA looping at low salt may cause stabilization of the 1:1 lac repressor: operator complex.
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    • of special interest. This paper together with [39] describes protein - DNA cross linking experiments for the lac repressor. The authors suggest that the presence of inducer causes the HTH motifs to reverse their orientation.
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    • The side-chain of the amino acid in position 110 of the lac repressor influences its allosteric equilibrium
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    • of outstanding interest Suckow J, Markiewicz P, Kleina LG, Miller J, Kisters-Woike B, Müller-Hill B. Genetic studies of the lac repressor XV: 4000 single amino acid substitutions and the analysis of the resulting phenotypes on the basis of the protein structure. of special interest J Mol Biol. 261:1996;509-523 The authors present an interesting review of the structural basis for the extensive genetic data on lac repressor using the crystal structure of PurR - hypoxanthine - DNA [8] and the core lac repressor structure bound to IPTG [4].
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