The side-chain of the amino acid residue in position 110 of the Lac repressor influences its allosteric equilibrium

Journal of Molecular Biology

Volumn 257, Issue 3, 1996, Pages 473-478

  Müller Hartmann, Herbert ^a   Müller Hill, Benno ^a  

^a UNIVERSITY OF COLOGNE   (Germany)

Author keywords

Conformational equilibrium; Induction; Lac repressor; Point mutation

Indexed keywords

ALANINE; AMINO ACID; ISOPROPYL THIOGALACTOSIDE; LYSINE; REPRESSOR PROTEIN; THREONINE;

ALLOSTERISM; AMINO ACID SUBSTITUTION; ARTICLE; CONTROLLED STUDY; DNA BINDING; ESCHERICHIA COLI; GENE EXPRESSION REGULATION; GENE SEQUENCE; GENE STRUCTURE; LACTOSE OPERON; MOLECULAR CLONING; NONHUMAN; OPERATOR; PHENOTYPE; PRIORITY JOURNAL; PROTEIN CONFORMATION; REPRESSOR GENE;

ESCHERICHIA COLI;

EID: 0040085980     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.1996.0176     Document Type: Editorial

References (19)

1. Alberti, S., Oehler, S., von Wilcken-Bergmann, B., Krämer, H. & Müller-Hill, B. (1991). Dimer-to-tetramer assembly of Lac repressor involves a leucine heptad repeat. New Biol. 3, 57-62.
2. Fickert, R. & Müller-Hill, B. (1992). How Lac repressor finds lac operator in vitro. J. Mol. Biol. 226, 59-68.
3. Friedman, A. M., Fischmann, T. O. & Steitz, T. A. (1995). Crystal structure of Lac repressor core tetramer and its implication for DNA looping. Science, 268, 1721-1727.
4. Gilbert, W. & Müller-Hill, B. (1966). Isolation of the Lac repressor. Proc. Natl Acad. Sci. USA, 56, 1891-1898.
5. Gilbert, W. & Müller-Hill, B. (1967). The lac operator is DNA. Proc. Natl Acad. Sci. USA, 58, 2415-2421.
6. Lehming, N., Sartorius, J., Niemöller, M., Genenger, G., von Wilcken-Bergmann, B. & Müller-Hill, B. (1987). The interaction of the recognition helix of Lac repressor with lac operator. EMBO J. 6, 3145-3153.
7. Lehming, N., Sartorius, J., Kisters-Woike, B., von Wilcken-Bergmann, B. & Müller-Hill, B. (1990). Mutant Lac repressors with new specificities hint at rules for protein-DNA recognition. EMBO J. 9, 615-621.
8. Markiewicz, P., Kleina, L. G., Cruz, C., Ehret, S. & Miller, J. H. (1994). Genetic studies of the Lac repressor. 14. Analysis of 4000 altered Escherichia coli Lac repressors reveals essential and non-essential residues, as well as 'spacers' which do not require a specific sequence. J. Mol. Biol. 240, 421-433.
9. Miller, J. H. (1972). Experiments in Molecular Genetics, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
10. Müller-Hill, B., Beyreuther, K. & Gilbert, W. (1971). Lac repressor from Escherichia coli. Methods Enzymol. 21, 483-487.
11. Newcomer, M. E., Lewis, B. A. & Quiocho, F. A. (1981). The radius of gyration of 1-arabinose-binding protein decreases upon binding of ligand. J. Biol. Chem. 256, 13218-13222.
12. Oehler, S., Eismann, E. R., Krämer, H. & Müller-Hill, B. (1990). The three operators of the lac operon cooperate in repression. EMBO J. 9, 973-979.
13. Oehler, S., Amouyal, M., Kolkhof, P, von Wilcken-Bergmann, B. & Müller-Hill, B. (1994). Quality and position of the three lac operators of E. coli define efficiency of repression. EMBO J. 13, 3348-3355.
14. Remaut, E., Stanssens, P. & Fiers, W. (1981). Plasmid vectors for high efficiency expression controlled by the PL promoter of the coliphage lambda. Gene, 15, 81-93.
15. Riggs, A. D., Newby, R. F. & Bourgeois, S. (1970). Lac repressor-operator interaction. 2. Effect of galactosides and other ligands. J. Mol. Biol. 51, 303-314.
16. Sambrook, J., Fritsch, E. F. & Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
17. Schumacher, M. A., Choi, K. Y., Zalkin, H. & Brennan, R. G. (1994). Crystal structure of LacI member, PurR, bound to DNA: minor groove binding by α helices. Science, 266, 763-770.
18. Schumacher, M. A., Choi, K. Y., Lu, F., Zalkin, H. & Brennan, R. G. (1995). Mechanism of corepressor-mediated specific DNA binding by the purine repressor. Cell, 83, 147-155.
19. Weickert, M. J. & Adhya, S. (1992). A family of bacterial regulators homologous to Gal and Lac repressors. J. Biol. Chem. 267, 15869-15874.