Inhibition of bovine endothelial cell activation in vitro by regulated expression of a transdominant inhibitor of NF-κB

Journal of Clinical Investigation

Volumn 99, Issue 4, 1997, Pages 763-772

  Anrather, Josef ^a   Csizmadia, Vilmos ^a   Brostjan, Christine ^a   Soares, Miguel P ^a   Bach, Fritz H ^a   Winkler, Hans ^a,b  

^a BETH ISRAEL DEACONESS MEDICAL CENTER   (United States)

^b Immunobiology Research Center   (United States)

Author keywords

adhesion molecules; endothelial cells; NF B; stable transfection; tetracycline regulation

Indexed keywords

IMMUNOGLOBULIN ENHANCER BINDING PROTEIN;

ANIMAL CELL; ARTICLE; CATTLE; CELL ACTIVATION; CONTROLLED STUDY; ENDOTHELIUM CELL; GENE EXPRESSION REGULATION; GENETIC TRANSFECTION; NONHUMAN; PRIORITY JOURNAL; VASCULAR ENDOTHELIUM;

EID: 0031027505     PISSN: 00219738     EISSN: None     Source Type: Journal    
DOI: 10.1172/JCI119222     Document Type: Article

References (73)

1. Weller, A., S. Isenmann, and D. Vestweber. 1992. Cloning of the mouse endothelial selectins. Expression of both E-and P-selectin is inducible by tumor necrosis factor α. J. Biol. Chem. 267:15176-15183.
2. Wertheimer, S.J., C.L. Myers, R.W. Wallace, and T.P. Parks. 1992. Intercellular adhesion molecule-1 gene expression in human endothelial cells. Differential regulation by tumor necrosis factor-α and phorbol myristate acetate. J. Biol. Chem. 267:12030-12035.
3. Swerlick, R.A., K.H. Lee, L.J. Li, N.T. Sepp, S.W. Caughman, and T.J. Lawley. 1992. Regulation of vascular cell adhesion molecule 1 on human dermal microvascular endothelial cells. J. Immunol. 149:698-705.
4. Howells, G.L., D. Chantry, and M. Feldmann. 1988. Interleukin 1 (IL-1) and tumor necrosis factor synergise in the induction of IL-1 synthesis by human vascular endothelial cells. Immunol. Lett. 19:169-173.
5. Chen, C.C., and A.M. Manning. 1996. TGFβ1, Il-10 and Il-4 differentially modulate the cytokine-induced expression of Il-6 and Il-8 in human endothelial cells. Cytokine. 8:58-65.
6. Rollins, B.J., T. Yoshimura, E.J. Leonard, and J.S. Pober. 1990. Cytokine-activated human endothelial cells synthesize and secrete a monocyte chemoattractant, MCP-1/JE. Am. J. Pathol. 136:1229-1233.
7. Moll, T., M. Czyz, H. Holzmüller, R. Hofer-Warbinek, E. Wagner, H. Winkler, F.H. Bach, and E. Hofer. 1995. Regulation of the tissue factor promoter in endothelial cells: binding of NF-κB-, AP-1-, and Sp1-like transcription factors. J. Biol. Chem. 270:3849-3857.
8. Bach, F.H., S.C. Robson, C. Ferran, H. Winkler, M.T. Millan, K.M. Stuhlmeier, B. Vanhove, M.L. Blakely, W.J. van der Werf, E. Hofer, R. de Martin, and W.W. Hancock. 1994. Endothelial cell activation and thromboregulation during xenograft rejection. Immunol. Rev. 141:5-30.
9. Hiscott, J., J. Marois, J. Garoufalis, M. Daddario, A. Roulston, I. Kwan, N. Pepin, J. Lacoste, H. Nguyen, G. Bensi, and M. Fenton. 1993. Characterization of a functional NF-κB site in the human interleukin-1β promoter - evidence for a positive autoregulatory loop. Mol. Cell. Biol. 13:6231-6240.
10. Cogswell, J.P., M.M. Godlevski, G.B. Wisely, W.C. Clay, L.M. Leesnitzer, J.P. Ways, and J.G. Gray. 1994. NF-κB regulates IL-1β transcription through a consensus NF-κB binding site and a nonconsensus CRE-like site. J. Immunol. 153:712-723.
11. Matsusaka, T., K. Fujikawa, Y. Nishio, N. Mukaida, K. Matsushima, T. Kishimoto, and S. Akira. 1993. Transcription factors NF-IL6 and NF-κB synergistically activate transcription of the inflammatory cytokines, interleukin 6 and interleukin 8. Proc. Natl. Acad. Sci. USA. 90:10193-10097.
12. Shibanuma, M., T. Kuroki, and K. Nose. 1994. Inhibition by N-acetyl-L-cysteine of interleukin-6 mRNA induction and activation of NF-κB by tumor necrosis factor a in a mouse fibroblastic cell line, Balb/3T3. FEBS Lett. 353:62-66.
13. Kunsch, C., and C.A. Rosen. 1993. NF-κB subunit-specific regulation of the interleukin-8 promoter. Mol. Cell. Biol. 13:6137-6146.
14. Oliveira, I.C., N. Mukaida, K. Matsushima, and J. Vilcek. 1994. Transcriptional inhibition of the interleukin-8 gene by interferon is mediated by the NF-κB site. Mol. Cell. Biol. 14:5300-5308.
15. Ueda, A., K. Okuda, S. Ohno, A. Shirai, T. Igarashi, K. Matsunaga, J. Fukushima, S. Kawamoto, Y. Ishigatsubo, and T. Okubo. 1994. NF-κB and Sp1 regulate transcription of the human monocyte chemoattractant protein-1 gene. J. Immunol. 153:2052-2063.
16. Whelan, J., P. Ghersa, H.R. van Huijsduijnen, J. Gray, G. Chandra, F. Talabot, and J.F. De Lamarter. 1991. An NF-κB-like factor is essential but not sufficient for cytokine induction of endothelial leukocyte adhesion molecule 1 (ELAM-1) gene transcription. Nucleic Acids Res. 19:2645-2653.
17. Shu, H.B., A.B. Agranoff, E.G. Nabel, K. Leung, C.S. Duckett, A.S. Neish, T. Collins, and G.J. Nabel. 1993. Differential regulation of vascular cell adhesion molecule-1 gene expression by specific NF-κB subunits in endothelial and epithelial cells. Mol. Cell. Biol. 13:6283-6289.
18. Neish, A.S., M.A. Read, D. Thanos, R. Pine, T. Maniatis, and T. Collins. 1995. Endothelial interferon regulatory factor 1 cooperates with NF-κB as a transcriptional activator of vascular cell adhesion molecule 1. Mol. Cell. Biol. 15:2558-2569.
19. Voraberger, G., R. Schafer, and C. Stratowa. 1991. Cloning of the human gene for intercellular adhesion molecule 1 and analysis of its 5′-regulatory region. Induction by cytokines and phorbol ester. J. Immunol. 147:2777-2786.
20. Ledebur, H.C., and T.P. Parks. 1995. Transcriptional regulation of the intercellular adhesion molecule-1 gene by inflammatory cytokines in human endothelial cells: essential roles of a variant NF-κB site and p65 homodimers. J. Biol. Chem. 270:933-943.
21. Pan, J.L., and R.P. McEver. 1993. Characterization of the promoter for the human P-selectin gene. J. Biol. Chem. 268:22600-22608.
22. Parry, G.C., and N. Mackman. 1995. Transcriptional regulation of tissue factor expression in human endothelial cells. Arteriosclerosis Thromb. Vasc. Biol. 15:612-621.
23. Diaz-Meco, M.T., I. Dominguez, L. Sanz, P. Dent, J. Lozano, M.M. Municio, E. Berra, R.T. Hay, T.W. Sturgill, and J. Moscat. 1994. ζ-PKC induces phosphorylation and inactivation of IκB-α in vitro. EMBO J. 13:2842-2848.
24. Miyamoto, S., M. Maki, M.J. Schmitt, M. Hatanaka, and I.M. Verma. 1994. Tumor necrosis factor α-induced phosphorylation of IκB-α is a signal for its degradation but not dissociation from NF-κB. Proc. Natl. Acad. Sci. USA. 91:12740-12744.
25. DiDonato, J.A., F. Mercurio, and M. Karin. 1995. Phosphorylation of IκBα precedes but is not sufficient for its dissociation from NF-κB. Mol. Cell. Biol. 15:1302-1311.
26. Alkalay, I., A. Yaron, A. Hatzubai, S. Jung, A. Avraham, O. Gerlitz, I. Pashutlavon, and Y. Benneriah. 1995. In vivo stimulation of IκB-β phosphorylation is not sufficient to activate NF-κB. Mol. Cell. Biol. 15:1294-1301.
27. Traenckner, E.B.M., H.L. Pahl, T. Henkel, K.N. Schmidt, S. Wilk, and P.A. Baeuerle. 1995. Phosphorylation of human IκB-α on serines 32 and 36 controls IκB-α proteolysis and NF-κB activation in response to diverse stimuli. EMBO J. 14:2876-2883.
28. Chen, Z.J., J. Hagler, V.J. Palombella, F. Melandri, D. Scherer, D. Ballard, and T. Maniatis. 1995. Signal-induced site-specific phosphorylation targets IκB-α to the ubiquitin-proteasome pathway. Genes & Dev. 9:1586-1597.
29. McElhinny, J.A., S.A. Trushin, G.D. Bren, N. Chester, and C.V. Paya. 1996. Casein kinase II phosphorylates IκB-α at S 289, S 289, S 293, and T 291 and is required for its degradation. Mol. Cell. Biol. 16:899-906.
30. DiDonato, J., F. Mercurio, C. Rosette, J. Wuli, H. Suyang, S. Ghosh, and M. Karin. 1996. Mapping of the inducible IκB phosphorylation sites that signal its ubiquitination and degradation. Mol. Cell. Biol. 16:1295-1304.
31. Scherer, D.C., J.A. Brockman, Z.J. Chen, T. Maniatis, and D.W. Ballard. 1995. Signal-induced degradation of IκB-α requires site-specific ubiquitination. Proc. Natl. Acad. Sci. USA. 92:11259-11263.
32. Alkalay, I., A. Yaron, A. Hatzubai, A. Orian, A. Ciechanover, and Y. Benneriah. 1995. Stimulation-dependent IκB-α phosphorylation marks the NF-κB inhibitor for degradation via the ubiquitin-proleasome pathway. Proc. Natl. Acad. Sci. USA. 92:10599-10603.
33. Traenckner, E.B.M., and P.A. Baeuerle. 1995. Appearance of apparently ubiquitin-conjugated IκB-α during its phosphorylation-induced degradation in intact cells. J. Cell Sci. Suppl. 19:79-84.
34. Baldi, L., K. Brown, G. Franzoso, and U. Siebenlist. 1996. Critical role for lysines 21 and 22 in signal-induced, ubiquitin-mediated proteolysis of IκB-α. J. Biol. Chem. 271:376-379.
35. Chen, Z.J., L. Parent, and T. Maniatis. 1996. Site-specific phosphorylation of IκBα by a novel ubiquitination-dependent protein kinase activity. Cell. 84:853-862.
36. Roff, M., J. Thompson, M.S. Rodriguez, J.M. Jacque, F. Baleux, F. Arenzana-Seisdedos, and R.T. Hay. 1996. Role of IκB-α ubiquitination in signal-induced activation of NF-κB in vivo. J. Biol. Chem. 271:7844-7850.
37. Mellits, K.H., R.T. Hay, and S. Goodbourn. 1993. Proteolytic degradation of MAD3 (IκBα) and enhanced processing of the NF-κB precursor p105 are obligatory steps in the activation of NF-κB. Nucleic Acids Res. 21:5059-5066.
38. Henkel, T., T. Machleidt, I. Alkalay, M. Kronke, Y. Benneriah, and P.A. Baeuerle. 1993. Rapid proteolysis of IκB-α is necessary for activation of transcription factor NF-κB. Nature (Lond.). 365:182-185.
39. Traenckner, E.B.M., S. Wilk, and P.A. Baeuerle. 1994. A proteasome inhibitor prevents activation of NF-κB and stabilizes a newly phosphorylated form of IκB-α that is still bound to NF-κB. EMBO J. 13:5433-5441.
40. Brown, K., S. Gerstberger, L. Carlson, G. Franzoso, and U. Siebenlist. 1995. Control of IκB-α proteolysis by site-specific, signal-induced phosphorylation. Science (Wash. DC). 267:1485-1488.
41. Fujita, T., G.P. Nolan, S. Ghosh, and D. Baltimore. 1992. Independent modes of transcriptional activation by the p50 and p65 subunits of NF-κB. Genes & Dev. 6:775-787.
42. Lin, R.T., D. Gewert, and J. Hiscott. 1995. Differential transcriptional activation in vitro by NF-κB/Rel proteins. J. Biol. Chem. 270:3123-3131.
43. Fujita, T., G.P. Nolan, H.C. Liou, M.L. Scott, and D. Baltimore. 1993. The candidate proto-oncogene bcl-3 encodes a transcriptional coactivator that activates through NF-κB p50 homodimers. Genes & Dev. 7:1354-1363.
44. Caamano, J.H., P. Perez, S.A. Lira, and R. Bravo. 1996. Constitutive expression of Bcl-3 in thymocytes increases the DNA binding of NF-κB1 (p50) homodimers in vivo. Mol. Cell. Biol. 16:1342-1348.
45. Schmitz, M.L., M.A. dos Santos Silva, H. Altmann, M. Czisch, T.A. Holak, and P.A. Baeuerle. 1994. Structural and functional analysis of the NF-κB p65 C-terminus: an acidic and modular transactivalion domain with the potential to adopt an α-helical conformation. J. Biol. Chem. 269:25613-25620.
46. Bours, V., V. Azarenko, E. Dejardin, and U. Siebenlist. 1994. Human RelB (I-Rel) functions as a κB site-dependent transactivating member of the family of Rel-related proteins. Oncogene. 9:1699-1702.
47. Ferran, C., M.T. Millan, V. Csizmadia, J.T. Cooper, C. Brostjan, F.H. Bach, and H. Winkler. 1995. Inhibition of NF-κB by pyrrolidine dithiocarbamate blocks endothelial cell activation. Biochem. Biophys. Res. Commun. 214: 212-223.
48. Brisseau, G.F., A.P.B. Dackiw, P.Y.C. Cheung, N. Christie, and O.D. Rotstein. 1995. Posttranscriptional regulation of macrophage tissue factor expression by antioxidants. Blood. 85:1025-1035.
49. Finco, T.S., A.A. Beg, and A.S. Baldwin. 1994. Inducible phosphorylation of IκB-α is not sufficient for its dissociation from NF-κB and is inhibited by protease inhibitors. Proc. Natl. Acad. Sci. USA. 91:11884-11888.
50. Sun, S.C., J. Elwood, C. Beraud, and W.C. Greene. 1994. Human T cell leukemia virus typeI Tax activation of NF-κB/Rel involves phosphorylation and degradation of IκB-α and RelA (p65)-mediated induction of the c-rel gene. Mol. Cell. Biol. 14:7377-7384.
51. Read, M.A., A.S. Neish, F.W. Luscinskas, V.J. Palombella, T. Maniatis, and T. Collins. 1995. The proteasome pathway is required for cytokine-induced endothelial-leukocyte adhesion molecule expression. Immunity. 2:493-506.
52. Brostjan, C., J. Anrather, V. Csizmadia, D. Stroka, M. Soares, F.H. Bach, and H. Winkler. 1996. Glucocorticoid-mediated repression of NF-κB activity in endothelial cells does not involve induction of IκBα synthesis. J. Biol. Chem. 271:19612-19617.
53. Wrighton, C.J., R. Hofer-Warbinek, T. Moll, R. Eytner, F.H. Bach, and R. de Martin. 1996. Inhibition of endothelial cell activation by adenovirus-mediated expression of IκBα, an inhibitor of NF-κB. J. Exp. Med. 183:1013-1022.
54. Pan, J.L., and R.P. McEver. 1995. Regulation of the human P-selectin promoter by Bcl-3 and specific homodimeric members of the NF-κB/Rel family. J. Biol. Chem. 270:23077-23083.
55. Kaplan, J.M., J.A. St. George, S.E. Pennington, L.D. Keyes, R.P. Johnson, S.C. Wadsworth, and A.E. Smith. 1996. Humoral and cellular immune responses of nonhuman primates to long-term repeated lung exposure to Ad2/ CFTR-2. Gene Ther. 3:117-127.
56. Zabner, J., B.W. Ramsey, D.P. Meeker, M.L. Aitken, R.P. Balfour, R.L. Gibson, J. Launspach, R.A. Moscicki, S.M. Richards, T.A. Standaert, et al. 1996. Repeat administration of an adenovirus vector encoding cystic fibrosis transmembrane conductance regulator to the nasal epithelium of patients with cystic fibrosis. J. Clin. Invest 97:1504-1511.
57. Ruben, S.M., P.J. Dillon, R. Schreck, T. Henkel, C.H. Chen, M. Maher, P.A. Baeuerle, and C.A. Rosen. 1991. Isolation of a Rel-related human cDNA that potentially encodes the 65-kD subunit of NF-κB. Science (Wash. DC). 254: 11-15.
58. Evan, G.I., G.K. Lewis, G. Ramsay, and J.M. Bishop. 1985. Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product. Mol. Cell. Biol. 5:3610-3616.
59. Gossen, M., and H. Bujard. 1992. Tight control of gene expression in mammalian cells by tetracycline-responsive promoters. Proc. Natl. Acad. Sci. USA. 89:5547-5551.
60. de Martin, R., B. Vanhove, Q. Cheng, E. Hofer, V. Csizmadia, H. Winkler, and F.H. Bach. 1993. Cytokine-inducible expression in endothelial cells of an IκBα-like gene is regulated by NF-κB. EMBO J. 12:2773-2779.
61. Brostjan, C., J. Anrather, V. Csizmadia, G. Natarajan, F.H. Bach, and H. Winkler. 1996. Deletion analysis of the porcine E-selectin promoter. Transplant Proc. 28:649-651.
62. Cooper, J.T., D.M. Stroka, C. Brostjan, A. Palmetshofer, F.H. Bach, and C. Ferran. 1996. A20 blocks endothelial cell activation through a NF-κB-dependent mechanism. J. Biol. Chem. 271:18068-18073.
63. de Wet, J.R., K.V. Wood, M. DeLuca, D.R. Helinski, and S. Subramani. 1987. Firefly luciferase gene: structure and expression in mammalian cells. Mol. Cell. Biol. 7:725-737.
64. Rosenthal, N. 1987. Identification of regulatory elements of cloned genes with functional assays. Methods Enzymol. 152:704-720.
65. Seed, B., and J.Y. Sheen. 1988. A simple phase-extraction assay for chloramphenicol acyltransferase activity. Gene (Amst.). 67:271-277.
66. Subramaniam, M., D. Colvard, P.E. Keeting, K. Rasmussen, B.L. Riggs, and T.C. Spelsherg. 1992. Glucocorticoid regulation of alkaline phosphatase, osteocalcin, and proto-oncogenes in normal human osteoblast-like cells. J. Cell. Biochem. 50:411-424.
67. Dyer, R.B., and N.K. Herzog. 1995. Isolation of intact nuclei for nuclear extract preparation from a fragile B-lymphocyte cell line. Biotechniques. 19: 192-195.
68. Leung, K., and G.J. Nabel. 1988. HTLV-1 transactivator induces interleukin-2 receptor expression through an NF-κB-like factor. Nature (Lond.). 333:776-778.
69. Zimmermann, K., M. Dobrovnik, C. Ballaun, D. Bevec, J. Hauber, and E. Böhnlein. 1991. Trans-activation of the HIV-1 LTR by the HIV-1 Tat and HTLV-I Tax proteins is mediated by different cis-acting sequences. Virology. 182:874-878.
70. Gossen, M., S. Freundlieb, G. Bender, G. Müller, W. Hillen, and H. Bujard. 1995. Transcriptional activation by tetracyclines in mammalian cells. Science (Wash. DC). 268:1766-1769.
71. Furth, P.A., L. Stonge, H. Boger, P. Gruss, M. Gossen, A. Kistner, H. Bujard, and L. Hennighausen. 1994. Temporal control of gene expression in transgenic mice by a tetracycline-responsive promoter. Proc. Natl. Acad. Sci. USA. 91:9302-9306.
72. Cheng, Q., C.A. Cant, T. Moll, R. Hofer-Warbinek, E. Wagner, M.L. Birnstiel, F.H. Bach, and R. de Martin. 1994. NF-κB subunit-specific regulation of the IκBα promoter. J. Biol. Chem. 269:13551-13557.
73. Beg, A.A., W.C. Sha, R.T. Bronson, S. Ghosh, and D. Baltimore. 1995. Embryonic lethality and liver degeneration in mice lacking the RelA component of NF-κB. Nature (Lond.). 376:167-170.