The role of antibodies to DNA in systemic lupus erythematosus - A review and introduction to an international workshop on DNA antibodies held in London, May 1996

Lupus

Volumn 6, Issue 3, 1997, Pages 290-304

  Isenberg, D A ^a,b   Ravirajan, C T ^a   Rahman, A ^a   Kalsi, J ^a  

^a UNIVERSITY COLLEGE LONDON   (United Kingdom)

^b Arthur Stanley House   (United Kingdom)

Author keywords

DNA antibodies; Immunoglobulin genes; SLE

Indexed keywords

DNA ANTIBODY;

ANTIBODY PRODUCTION; ANTIBODY STRUCTURE; CONFERENCE PAPER; DISEASE MODEL; IMMUNOGLOBULIN GENE; PATHOGENICITY; PRIORITY JOURNAL; SEQUENCE ANALYSIS; SOMATIC MUTATION; SYSTEMIC LUPUS ERYTHEMATOSUS;

EID: 0030975249     PISSN: 09612033     EISSN: None     Source Type: Journal    
DOI: 10.1177/096120339700600316     Document Type: Conference Paper

References (146)

1. Cepellini R, Polli E, Celada F. A DNA-reacting factor in serum of a patient with lupus erythematosus diffuses. Proc Soc Exp Biol Med 1957; 76: 572-574.
2. Meischer P, Strassie R. New serological methods for the detection of the LE factor. Vox Sanguins 1957; 2. 283.
3. Robbins WC, Holman HR, Deicher H, Kunkel HG. Complement fixation with cell nuclei and DNA in lupus erythematosus. Proc Soc Miol Med 1957; 96: 575-579.
4. Seligmann M. Mise en evidence dans le serum des malades atteints de lupus erythmateux dissemine de precipitation avec l'acide desoxyribonucleique. Comptes Rendus de l'Academie des Sciences (Paris) 1957; 245: 243-245.
5. Cervera R et al. Systemic lupus erythematosus: clinical and immunolgic patterns of disease expression in a cohort of 1000 patients. Medicine (Baltimore) 1993; 72: 113-124.
6. Duncan R, McConkey EH. How many proteins are there in a typical mammalian cell?. Clin Chem 1982; 28: 749-755.
7. Gharavi AE, Chu JL, Elkon KB. Autoantibodies to intra-cellular proteins in human systemic lupus erythematosus are not due to random polyclonal B cell activation. Arthritis Rheum 1988; 11: 1337-1345.
8. Swaak AJG, Aarden LA, Statuis van Eps LW, Feltkamp TEW. Anti-dsDNA and complement profiles as prognostic guides in systemic lupus erythematosus. Arthritis Rheum 1979; 22: 226-235.
9. Lloyd W, Schur PH. Immune complexes, complement and anti-DNA in exacerbations of systemic lupus erythematosus (SLE). Medicine (Baltimore) 1981; 60: 208-217.
10. Ter Borg EJ et al. Measurements of increases in anti-double stranded DNA antibody levels as a predictor of disease exacerbation in systemic lupus erythematosus. Arthritis Rheum 1990; 33: 634-643.
11. Okamura M et al Significance of enzyme linked immunosorbent assay [ELISA] for antibodies to double stranded and single stranded DNA in patients with lupus nephritis: correlation with severity of renal histology. Ann Rheum Dis 1993; 52: 14-20.
12. Bootsma H et al. Prevention of relapses in systemic lupus erythematosus. Lancet 1995; 345: 1595-1599.
13. Isenberg DA, Garton M, Reichlin MW, Reichlin M. Long term follow up of autoantibody profiles in black female lupus patients and clinical comparison with Caucasian and Asian patients. Brit J Rheum In press.
14. Koffler D, Schur PH, Kunkel HG. Immunological studies concerning the nephritis of systemic lupus erythematosus J Exp Med 1967; 126: 607-624.
15. Pankewycz OG, Migliorini P, Madaio MP. Polreactive autoantibodies are nephritogemc in murine lupus nephritis. J Immunol 1987; 139: 3287-3294.
16. Isenberg DA et al. Detection of cross-reactive anti-DNA antibody idiotypes in the serum of lupus patients and their relatives. Arthritis Rheum 1985; 28: 999-1007.
17. Andrezewski C et al. Antigen binding diversity and idiotypic cross-reactions among hybridoma antibodies to DNA. 1981; 236: 226-231.
18. Ravirajan C et al. Antigen binding diversity of human hybridoma autoantibodies derived from splenocytes of patients with SLE. Lupus 1992; 1: 167-174.
19. Jacob L, Tron F, Bach JF, Louvard D. A monoclonal anti-DNA antibody also binds to cell surface proteins. Proc Natl Acad Sci USA 1984, 81: 3843-3845.
20. Naparstek Y et al. Immunochemical similarities between monoclonal antibacterial Waldenstrom's macroglobulins and monoclonal anti-DNA lupus autoantibodies. J Exp Med 1985; 161: 1525-1538.
21. Shoenfeld Y et al. Production of autoantibodies by human-human hybridomas. J Clin Invest 1982; 70: 205-208.
22. Stollar BD. Antibodies to DNA. CRC Crit Rev Biochem 1986; 20, 1-36.
23. Rauch J, Tannenbaum H, Stollar BD. Schwartz RS. Monoclonal anti-cardiolipin antibodies bind to DNA. Eur J Immunol 1984; 14: 529-534.
24. Isenberg DA, Ehrenstein MR, Longhurst C, Kalsi J. The origin, sequence, structure and consequences of developing anti-DNA antibodies - a human perspective. Arthritis Rheum 1994; 37: 169-180.
25. Gilkeson GS, Grudier J, Karounos D, Pisetsky D Induction of anti-dsDNA antibodies in normal mice by immunization with bacterial DNA J Immunol 1989; 142: 1482-1486.
26. Gilkeson GS, Bloom DB, Pistesky DS, Clarke SK Molecular characterisation of anti-DNA antibodies induced in normal mice by immunization with bacterial DNA. J Immunol 1993; 151: 1353-1364
27. 1 hybrids but not in normal mice. Eur J Immunol 1994; 24: 66-70.
28. Krishnan MR, Marion TN. Structural similarity of antibody variable regions from immune and autoimmune anti-DNA antibodies. J Immunol 1993; 150: 4948-4957.
29. Bell DA, Morrison B, Vanden-Bygaert P. Immunogenic DNA related factors: nucleosomes spontaneously released from normal mouse lymphoid cells stimulate proliferation and immunoglobulin synthesis of normal mouse lymphocytes. J Clin Invest 1990; 85: 1487-1490.
30. Ehrenstein MR, Longhurst C, Isenberg DA. Production and isotype switching of monoclonal anti-DNA antibodies from SLE patients. Clin Exp Immunol 1993; 92: 39-45.
31. Gordon C, Blount S, Lunec J, Emery P. Measurement of antibodies to human DNA denatured by reactive oxygen species provides a new and more sensitive test for the diagnosis of SLE (abstract). Br J Rheum 1990; 39: S42.
32. Emlin W, Neibur J, Kadera R. Accelerated in vitro apoptosis of lymphocytes from patients with systemic lupus erythematosus. J Immunol 1994; 152: 3685-3692.
33. Williams W, Isenberg DA. Idiotypes and autologous anti-idiotypes in human autoimmune disease - some theoretical and practical observations. Autoimmunity 1994; 17. 343-352.
34. Theofilipoulos AN, Dixon FJ. Murine models of systemic lupus erythematosus. Adv Immunol 1985; 37: 269-390.
35. Datta SK et al. Genetic studies of autoimmunity and retrovirus expression in crosses of New Zealand Black mice. I. Xenotropic virus. J Exp Med 1978; 147: 854-871.
36. Andrews B et al. Spontaneous murine lupus-like syndromes. Clinical and immunopathological manifestatins in several strains. J Exp Med 1978; 148: 1198-1215.
37. Schiffenbauer J et al. A unique sequence of the NZW I-E beta chain and its possible contribution to autoimmunity in the (NZB × NZW) F1 mouse. J Exp Med 1989; 170: 971-984.
38. Cohen PL, Eisenberg RA. lpr and gld: single gene models of systemic autoimmunity and lymphoproliferative disease. Ann Rev Immunol 1991; 9: 243-269
39. Bhandoola A et al gld and lpr mice: single gene mutant models for failed self tolerance. Int Rev Immunol 1994; 11: 231-244.
40. Schwartz RH. Acquisition of immunolgic self-tolerance. Cell 1989; 57: 1079-1081.
41. Murphy ED, Roths JB Autoimmunity and lymphoproliferation: induction by mutant gene lpr, and acceleration by male-associated factor in strain BXSB mice. In: Rose NR, Bigazzi PE, Warner NL (eds). Genetic Control of Autoimmune Disease Elsevier: New York, 1978; pp 207-221.
42. Cohen PL, Eisenberg RA. The lpr and gld genes in systemic autoimmunity: life and death in the Fas lane. Immunol Today 1992; 13: 427-428.
43. Smith CA et al. Antibodies to CD3/T cell receptor complex induce death by apoptosis in immature T cells in thymic culture. Nature 1989; 337: 181-184.
44. Liu Y-J et al. Mechanism of antigen-driven selection in germinal centres. Nature 1989; 342: 929-931.
45. Kabelitz D, Phol T, Pechhold K. Activation-induced cell death (apoptosis) of mature peripheral T lymphocytes Immunol Today 1993; 14: 338-339.
46. Von Boehmer HA. The developmental biology of T lymphocytes. Annu Rev Immunol 1986; 6: 309-326.
47. Watanabe-Fukunaga R et al. The cDNA structure, expression, and chromosal assignment of the mouse Fas antigen. J Immunol 1992; 148: 1274-1279.
48. Drappa J, Brot N, Elkon KB. Fas protein is expressed at high levels on CD4+CD8+ thymocytes and activated mature lymphocytes in normal mice but not in the lupus-prone strain, MRL-lpr/lpr. Proc Natl Acad Sci USA 1993; 90: 10340-10344.
49. Miyawaki T et al. Differential expression of apoptosis-related Fas antigen on lymphocyte subpopulations in human peripheral blood. J Immunol 1992; 149: 3753-3758.
50. Owen-Schaub LB, Yonehara S, Crump III Wl, Grim EA. DNA fragmentation and cell death is selectively triggered in activated human lymphocytes by Fas antigen engagement. Cell Immunol 1992; 140: 197-205.
51. Watanabe-Fukunaga R et al. Lymphoproliferation disorder in mice explained by defects in Fas antigen that mediates apoptosis. Nature 1992; 356: 314-317.
52. Itoh N et al. The polypeptide encoded by the cDNA for human cell surface antigen Fas can mediate apoptosis. Cell 1991; 66: 233-243.
53. Nagata S, Suda T. Fas and Fas ligand: lip and gld mutations. Immunol Today 1995; 16. 39-43.
54. Ravirajan CT et al. An analysis of apoptosis in lymphoid organs and lupus disease in murine systemic lupus erythematosus. Clin Exp Immunol 1996; 105. 306-312.
55. Steinberg AD. Concepts of pathogenesis of systemic lupus erythematosus. Clin Immunol Immunopathol 1992; 63: 19-29
56. Sobel ES et al. The abnormal lpr double negative T cell fails to proliferate in vivo. Clin Immunol Immunopathol 1995; 74: 177-184.
57. Van Houten N, Budd RC. Lessons from the lpr mouse - T lymphocyte development. Seminars in Immunol 1994; 6: 1-2.
58. Steinberg AD. MRL-lpr/lpr disease: theories meet Fas Seminars in Immunol 1994; 55-69.
59. Rozzo SJ, Eisenberg RA, Cohen PL, Kotzin BL. Development of the T cell receptor repertoire in lpr mice. Seminare in Immunol 1994; 6: 19-26.
60. Ray S, Diamond B. Generation of a fusion partner to sample the repertoire of splenic B cells destined for apoptosis. Proc Natl Acad Sci USA 1994, 91: 5548-5551.
61. Theofilopoulous AN et al. A comparative immunologic analysis of several murine strains with autoimmune manifestations. Clin Immunol Immunopathol 1980; 15: 258-278.
62. Eisenberg RA et al. The role of B cell abnormalities in systemic autoimmune syndromes of lpr and gld mice. Seminars in Immunol 1994; 6: 49-54.
63. Datta SK et al. Pathogenic anti-DNA antibodies and pathogenic autoantibody-inducing T cells. Immunol Ser 1991; 55: 133-153
64. Wofsy D, Seaman WE. Reversal of advanced murine lupus in NZB/NZW F1 mice by treatment with monoclonal antibody to L3T4. J Immunol 1987; 138 3247-3253.
65. Prud'Home EJ et al. Identification of a B cell differentiation factor(s) spontaneously produced by proliferating T cells in murine lupus strains of lpr/lpr genotype. J Exp Med 1983; 157: 730-735.
66. Gavalchin J, Datta SK. The NZB and SWR model of lupus nephritis. II. Autoantibodies deposited in renal lesions show a distinctive and restricted idiotypic diversity. J Immunol 1987; 138: 138-162.
67. Papoian R, Pillarisetty R, Talal N. Immunological regulation of spontaneous antibodies to DNA and RNA II. Sequential switch from IgM to IgG in NZB/NZWF1 mice. Immunology 1974; 32 75-88.
68. Datta SK, Patel H, Berry D. Induction cationic shift in IgG anti-DNA antibodies. Role of T helper cells with classical and noval phenotypes in three murine models of lupus nephritis. J Exp Med 1987; 165: 1252-1265.
69. Gallo GR, Caulin-Glaser T, Lamm ME. Charge of circulating immune complexes as a factor in glomerular basement localization in mice. J Clin Invest 1981; 67: 4209-4218.
70. Stollar BD Nucleic acids as antigens. In Sela M (ed). The Antigens Academic Press: New York, 1973; pp 1-85.
71. Schwartz RS, Stollar BD. Origins of anti-DNA antibodiesJ Clin Invest 1985; 75: 321-327.
72. Madaio MP, Hodder S, Schwartz R, Stollar BD. Responsiveness of autoimmune and normal mice to nucleic acid antigens. J Immunol 1984; 132: 872-876.
73. Davis P, Russell AS, Percy JS. Antibodies to UV light denatured DNA in SLE. J Rheumatol 1976; 3: 375-379.
74. Lafer EM et al. Antibodies specific for left-handed Z-DNA. Proc Nat Acad Sci USA 1981; 78: 3546-3550.
75. Shlomchik MJ et al. Anti-DNA antibodies from autoimmune mice arise by clonal expansion and somatic mutation. J Exp Med 1990; 171. 265-297.
76. Rekvig OP et al. Molecular analyses of anti-DNA antibodies induced by polyomavirus BK in BALB/c mice. Scand J Immunol 1995; 41. 593-602.
77. Limpanasithikul W, Ray S, Diamond B. Cross-reactive antibodies have protective and pathogenic and pathogenic potential. J Immunol 1995; 155: 967-973.
78. Stetler DA, Jacob ST. Immunization of rabbits with purified RNA polymerase I induces a distinct population of antibodies against nucleic acids as well as antipolymerase I antibodies, both characteristics of systemic lupus erythematosus. Proc Natl Acad Sci USA 1985; 82: 6779-6789.
79. Gilkeson GS, Pritchard AJ, Pisetsky DS. Specificity of antiDNA antibodies induced in normal mice by immunization with bacterial DNA. Clin Immunol Immunopath 1991; 59. 288-300
80. Atanassov C et al. New Zealand white rabbits immunized with RNA-complexed total histones developed an autoimmune-like response. Clin Exp Immunol 1991; 86: 124-133.
81. Ravirajan CT, Muller S, Katz DR, Isenberg DA. Effects of histone and RNA-histone complexes on the disease process of murine SLE. Autoimmunity 1995; 21: 117-122.
82. Desai DD, Krishnan MR, Swindle JT, Marion TN. Antigen-specific induction of antibodies against native mammalian DNA in non-autoimmune mice. J Immunol 1993; 151: 1614-1626.
83. Schwartz LM, Osborne BA. Programmed cell death, apoptosis and killer genes. Immunol Today 1993; 14: 582-590.
84. Burlingame RW, Rubin RL, Balderas RS, Theofilopoulus AN. Genesis and evolution of antichromatin autoantibodies in murine lupus implicates T-dependent immunization with self antigen. J Clin Invest 1993; 91: 1687-1696.
85. Amoura Z et al. Nucleosome-restricted antibodies are detected before anti-dsDNA and/or antihistone antibodies in serum of MRL-Mp lpr/lpr and +/+ mice, and are present in kidney eluates of lupus mice with proteinuria. Arthritis Rheum 1994; 37: 1684-1688.
86. Mohan C, Adams S, Stanik V, Datta SK. Nucleosome: a major immunogen for pathogenic autoantibody-inducing T cells of lupus. J Exp Med 1993; 177: 1367-1381.
87. Ehrenstein MR et al. Human IgG anti-DNA antibodies deposit in kidneys and induce proteinuria in SCID mice. Kidney Int 1995; 48: 705-711.
88. Ravirajan CT et al. Genetic, Structural and Functional properties of a human IgG anti-DNA autoantibody. Submitted.
89. Wu TT, Kabat EA. An analysis of the sequences of the variable regions of Bence Jones proteins and myeloma light chains and their implications for antibody complementarity. J Exp Med 1970; 132: 211-250.
90. Amzel LM et al. The three-dimensional structure of a combining region-ligand complex of immunoglobulin New at 3.4A resolution. Proc Natl Acad Sci USA 1974; 71: 1427-1430.
91. Herron JN et al. An autoantibody to single-stranded DNA: comparison of the three dimensional structures of the uliganded Fab and a deoxynucleotide-Fav complex. Proteins: Structure, Function and Genetics 1991; 11: 159-175.
92. Tonegawa S. Somatic generation of antibody diversity. Nature 1983; 302: 575-581.
93. Matsuda F et al. Structure and physical map of 64 variable segments in the 3′ 0.8-megabase region of the human immunoglobulin heavy chain locus. Nature Genetics 1993; 3: 88-94.
94. H locus completed by analysis of the telomeric region of chromosome 14q. Nature Genetics 1994; 7: 162-168.
95. Waisman A and Mozes E. Variable region sequences of autoantibodies from mice with experimental systemic lupus erythematosus. Eur J Immunol 1993; 23: 1566-1573.
96. Krishnan MR, Marion TN. Structural similarity of antibody variable regions from immune and autoimmune anti-DNA antibodies. J Immunol 1993; 150: 4948-4957.
97. Schlomchik M et al Anti-DNA antibodies from autoimmune mice arise by clonal expansion and somatic mutation J Exp Med 1990; 171: 265-297.
98. H gene analysis of spontaneously activated B cells in adult MRL lpr/lpr mice. J558 bias is not limited to classic lupus antibodies. J Immunol 1991; 147: 1504-1511.
99. Tillman DM, Jou NT, Marion TN. Both IgM and IgG anti-DNA antibody are the products of clonally selective B cell stimulation in (NZB × NZW) F1 mice. J Exp Med 1992; 176: 361-380.
100. Isenberg DA, Ehrenstein MR, Longhurst C, Kalsi JK. The origin, sequence, structure, and consequences of developing anti-DNA antibodies: a human perspective. Arthritis Rheum 1994; 37: 169-180.
101. Winkler TH, Fehr H, Kalden JR. Analysis of immunoglobulin variable region genes from human IgG anti-DNA hybridomas. Eur J Immunol 1992; 22: 1719-1728.
102. Paul E, Iliev AA, Livney A, Diamond B. The Anti-DNA Associated Idiotype 8.12 is Encoded by the VγII Gene Family and Maps to the Vicinity of L Chain CDR 1. J Immunol 1992; 149: 3588-3593.
103. H4-21 gene segment by anti-DNA antibodies from patients with SLE. J Autoimmunity 1993; 6: 809-826.
104. Ehrenstein MR, Longhurst CM, Latchman DS, Isenberg DA. Serological and genetic characterization of a human monoclonal immunoglobulin G anti-DNA idiotype. J Clin Invest 1994; 93: 1787-1797.
105. H repertoire. Immunol Today 1995; 16: 237-242.
106. Schroeder HW, Hillson JL, Perimutter RM Early restriction of the human antibody repertoire. Science 1987; 238: 791-793.
107. Schroeder HW and Wang JY. Preferntial utilization of conserved immunoglobulin heavy chain variable gene segments during human fetal life. Proc Natl Acad Sci USA 1990; 87: 6146-6150.
108. Dersimonian H, Schwartz RS, Barrett KJ, Stollar BD. Relationship of human variable region heavy chain germ-line genes to genes encoding anti-DNA autoantibodies. J Immunol 1987; 139: 2496-2501.
109. Siminovitch KA et al. A human anticardiolipin antibody is encoded by developmentally restricted heavy and light chain variable region genes. Autoimmunity 1990; 8: 97-105.
110. Blanco F et al. Identification and characterization of a new human DNA reactive monoclonal antibody and a common idiotype, WRI 176 Id β. Lupus 1994; 3: 15-24.
111. H-proximal human immunoglobulin heavy chain variable region gene. J Exp Med 1989; 170: 1347-1355
112. Neuberger MS, Milstein C. Somatic hypermutation. Curr Opin Immunol 1995; 7: 248-254.
113. Schlomchik MJ et al. The role of clonal selection and somatic mutation in autoimmunity. Nature 1987; 328: 805-811.
114. Stollar D. Molecular analysis of anti-DNA antibodies. FASEB J 1994; 8: 337-342.
115. van Es JH et al . Somatic mutations in the variable region of a human anti-dsDNA autoantibody suggest a role for antigen in the induction of systemic lupus erythematosus. J Exp Med 1991; 173: 461-470.
116. Huang C, Stollar BD. A majority of human IgH chain cDNA of normal human adult blood lymphocytes resembles cDNA for fetal Ig and natural autoantibodies. J Immunol 1993; 151: 5290-5300.
117. Rioux JD, Zdarsky E, Newkirk MM, Rauch J. Anti-DNA and anti-platelet specificities of SLE-derived autoantibodies: evidence for CDR2H mutations and CDR3H motifs. Mol Immunol 1995; 32: 683-696.
118. Manheimer-Lory A et al. Molecular characteristics of antibodies bearing an anti-DNA associated idiotype. J Exp Med 1991; 174: 1639-1652.
119. Longhurst C et al. Analysis of immunoglobulin variable genes of a human IgM anti-myeloperoxidase antibody derived from a patient with vasculitis. Immunology 1996; 87: 334-338.
120. Cairns E, Kwong PC, Misener VTP, Bell DA. Analysis of variable region genes encoding a human anti-DNA antibody of normal origin. J Immunol 1989; 143: 685-691.
121. Radie MZ et al. Residues that mediate DNA binding of autoimmune antibodies. J Immunol 1993, 150: 4966-4977.
122. Katz JB, Limpanasithikul W, Diamond B. Mutational analysis of an autoantibody differential binding and pathogenicity. J Exp Med 1994; 180: 925-932.
123. Pewzner-Jung Y, Simon T, Eilat D. Structural elements controlling anti-DNA antibody affinity and their relationship to anti-phosphorylcholme activity. J Immunol 1996; 156: 3065-3973.
124. Polymenis M, Stollar BD. Critical binding site amino acids of anti-Z-DNA single chain Fv molecules: role of heavy and light chain CDR3 and relationship to autoantibody activity. J Immunol 1994; 152: 5318-5329.
125. Barbas SM, Ditzel HJ, Salonen EM, Yang WP, Silverman GJ, Burton DR. Human autoantibody recognition of DNA. Proc Natl Acad Sci USA 1995; 92 2529-2933.
126. Putterman C, Limpanasithikul W, Edelman M, Diamond B. The double edged sword of the immune response. Mutational analysis of a murine anti-pneumococcal, anti-DNA antibody. J Clin Invest 1996; 97: 2251-2259.
127. Swanson PC et al. Ligand recognition by murine anti-DNA autoantibodies. II Genetic analysis and pathogenicity. J Clin Invest 1996; 97: 1748-1760.
128. Ohnishi K et al. Comparison of pathogenic and non-pathogenic murine antibodies to DNA: antigen binding and structural characteristics. Int Immunol 1994; 6: 817-830.
129. Burlingame RW, Boey ML, Starkebaum G, Rubin RL. The central role of chromatin in autoimmune responses to histones and DNA in systemic lupus erythematosus. J Clin Invest 1994; 94. 182-192.
130. Diamond B, Scharff MD. Somatic mutation of T15 heavy chain gives rise to an antibody with autoantibody specificity. Proc Natl Acad Sci USA 1984; 81: 5841-5844.
131. Amoura Z et al. Nucleosome-restricted antibodies are detected before anti-dsDNA and/or anti-histone antibodies in serum of MRL-Mp lpr/lpr and +/+ mice, and are present in kidney eluates of lupus mice with protemuria. Arthritis Rheum 1994; 37: 1684-1688.
132. Burlmghame RW, Rubin, RL, Balderas RS, Theopoulos AN. Genesis and evolution of anti-chromatin autoantibodies in munne lupus implicates T-dependent immunization with self-antigen. J Clin Invest 1993; 91: 1687-1696.
133. Radic MZ, Weigert M. Genetic and structural evidence for antigen selection of anti-DNA antibodies. Ann Rev Immunol 1994; 12: 487-520.
134. Tomlinson IM et al. The imprint of somatic hypermutation on the repertoire of human germline V genes. J Mol Biol 1996; 256: 813-817.
135. Gibson AL et al. Crystallographic characterization of the Fab fragment of a monoclonal anti ss-DNA antibody. Mol Immunol 1985; 22: 499-502.
136. Cygler M, Boodhoo A, Lee JS, Anderson WF. Crystallization and structure detemination of an autoimmune antipoly(dT) immunoglobulin Fab fragment at 3.0A resolution. J Biol Chem 1987; 262: 643-648.
137. Mol CD et al. Structure of an immunoglobulin Fab Fragment specific for triple-stranded DNA. J Biol Chem 1994, 269: 3615-3622.
138. Mol CD, Muir AKS, Lee JS, Anderson WF. Structure of an immunoglobulin Fab Fragment Specific for Poly(dG)·Poly(dC). J Biol Chem 1994; 269: 3605-3614.
139. Martin ACR, Cheetham JC, Rees AR. Molecular modelling of antibody combining sites. Meth Enz 1991; 203: 121-153.
140. Chothia C et al. Conformations of immunoglobulin hypervariable regions. Nature (london) 1989; 342: 877-883.
141. Chothia C et al. Structural repertoire of human VH segments. J Mol Biol 1992; 227: 799-817.
142. Martin ACR, Cheetham JC, Rees AR. Modelling antibody hypervariable loops: A combined algorithm. Proc Natl Acad Sci USA 1989; 86: 9268-9272.
143. Kalsi JK et al. Functional and modelling studies of the binding of human monoclonal anti-DNA antibodies to DNA. Mol Immunol 1996; 33: 471-483.
144. Stollar BD, Zon G, Parton RW. A recognition site on synthetic helical oligonucleotides for monoclonal anti-native DNA autoantibody. Proc Natl Acad Sci USA 1986; 83: 4469-4473.
145. Eagan MA, Norris JM, Cooper BC, Glick GD. Structural patterns in anti-DNA autoantibodies: A molecular modelling study. Bioorgan Chem 1995; 23: 482-498.
146. Barry MM, Mol CD, Anderson WF, Lee JS. Sequencing and modeling of anti-DNA immunoglobulin Fv domains. Comparison with crystal structures. J Biol Chem 1994; 269: 3623-3632.