Selective detection of the carbohydrate-bound state of concanavalin A at the single molecule level

Journal of the American Chemical Society

Volumn 119, Issue 33, 1997, Pages 7676-7684

  La Clair, James J ^a  

^a SCRIPPS RESEARCH INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CONCANAVALIN A; DYE; GLYCOCONJUGATE;

ARTICLE; BINDING AFFINITY; CHEMICAL BOND; FLUORESCENCE; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PHOTODEGRADATION; SYNTHESIS;

EID: 0030966276     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja964366g     Document Type: Article

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120. -1, as given by the product of its cross-sectional area and photon density.
121. The concentration provided is a description of the Con A-maltoside 1 complex which undergoes fluorescence. The total concentration of the Con A-maltoside 1 complex is much larger and requires knowledge of its quantum yield. Since there is no direct means to determine this yield, the affinity of this interaction cannot be calculated with this method.
122. Transients were also not detected in samples with concentrations of 2 ranging from 0.012 μM up to the point where the background was to large for the detector (∼200 μM) with and without Con A.
123. Wang, J. L.; Becker, J. W.; Reeke, J. N.; Edelman, G. M. Proc. Natl. Acad. Sci. U.S.A. 1971, 68, 1130.