Trafficking of the androgen receptor in living cells with fused green fluorescent protein-androgen receptor

Molecular and Cellular Endocrinology

Volumn 129, Issue 1, 1997, Pages 17-26

  Georget, V ^a   Lobaccaro, J M ^a   Terouanne, B ^a   Mangeat, P ^b   Nicolas, J C ^a   Sultan, C ^a,c  

^a INSERM   (France)

^b CNRS   (France)

^c LAPEYRONIE HOSPITAL   (France)

Author keywords

Androgen insensitivity syndrome; Androgen receptor; Antiandrogens; Intracellular reporter; Nuclear translocation

Indexed keywords

ANDROGEN; ANDROGEN RECEPTOR; ANTIANDROGEN; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN;

ANDROGEN INSENSITIVITY SYNDROME; ANIMAL CELL; ARTICLE; CELL NUCLEUS; CELLULAR DISTRIBUTION; CHIMERA; CONTROLLED STUDY; CYTOPLASM; GENETIC TRANSFECTION; HORMONE RECEPTOR INTERACTION; MOLECULAR DYNAMICS; NONHUMAN; PRIORITY JOURNAL; PROTEIN TRANSPORT; TEMPERATURE;

ANDROGENS; ANIMALS; BIOLOGICAL TRANSPORT, ACTIVE; CELL LINE; CELL NUCLEUS; COS CELLS; CYTOPLASM; FLUORESCENCE; GREEN FLUORESCENT PROTEINS; KINETICS; LUMINESCENT PROTEINS; RECEPTORS, ANDROGEN; RECOMBINANT FUSION PROTEINS; TRANS-ACTIVATION (GENETICS); TRANSFECTION;

EID: 0030948745     PISSN: 03037207     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0303-7207(97)04034-3     Document Type: Article

References (26)

1. Guiochon-Mantel, A., Delabre, K., Lescop, P. and Milgrom, E. (1996) Intracellular traffic of steroid hormone receptors. J. Steroid Biochem. Mol. Biol. 56, 1-6.
2. King, W.J. and Greene, G.L. (1984) Monolonal antibodies localize oestrogene receptor in the nuclei of target cells. Nature 307, 745-747.
3. Perrot-Applanat, M., Logeat, F., Groyer-Picard, M.T. and Milgrom, E. (1985) Irnmunocytochemical study of mammalian progesterone receptor using monoclonal antibodies. Endocrinology 116, 1473-1484.
4. Guiochon-Mantel, A., Lescop, P., Christin-Maitre, S., Loosfelt, H., Perrot-Applanat, M. and Milgrom, E. (1991) Nucleocytoplasmic shuttling of the progesterone receptor. EMBO J. 10, 3851-3859.
5. Lombes, M., Farman, N., Oblin, M.E., Baulieu, E.E., Bonvalet, J.P., Erlanger, B.F. and Gasc, J.M. (1990) Immunohistochemical localization of renal mineralocorticoid receptor by using an anti-idiotypic antibody that is an internal image of aldosterone. Proc. Natl. Acad. Sci. USA 87, 1086-1088.
6. Robertson, N.M., Schulman, G., Karnick, S., Alnemri, E. and Litwack, G. (1993) Demonstration of nuclear translocation of the mineralocorticoid receptor (MR) using an anti-MR antibody and confocal laer scanning microscopy. Mol. Endocrinol. 7, 1226-1239.
7. Martins, V.R., Pratt, W.B., Terracio, L., Hirst, M.A., Ringold, G.M. and Housley, P.R. (1991) Demonstration by confocal microscopy that unliganded overexpressed glucocorticoid receptors are distributed in a non random manner throughout all planes in the nucleus. Mol. Endocrinol. 5, 217-225.
8. Akner, G., Wikstrom, A.C. and Gustafsson, J.A. (1995) Subcellular distribution of the glucocorticoid receptor and evidence of its association with microtubules. J. Steroid Biochem. Mol. Biol. 52,1-16.
9. Brink, M., Humbel, B.M., De Kloet, E.R. and Van Driel, R. (1992) The unliganded glucocorticoid receptor is localized in the nucleus, not in the cytoplasm. Endocrinology 130, 3575-3581.
10. Sar, M., Lubahn, D.B., French, F.S. and Wilson, E.M. (1990) Immunohistochemical localization of the androgen receptor in rat and human tissues. Endocrinology 127, 3180-3186.
11. Husmann, D.A., Wilson, C.M., McPhaul, M.J., Tilley, W.D. and Wilson, J.D. (1990) Antipeptides antibodies to two distinct regions of the androgen receptor localize the receptor protein to the nuclei of target cells in the rat and human prostate. Endocrinology 126, 2359-2368.
12. Jenster, G., Trapman, J. and Brinkmann, A.O. (1993) Nuclear import of the human androgen receptor. Biochem. J. 293, 761-768.
13. Simental, J.A., Sar, M., Lane, M.V., French, F.S. and Wilson, E.M. (1991) Transcriptional activation and nuclear targeting signals of the human androgen receptor. J. Biol. Chem. 266, 510-518.
14. Chalfie, M., Tu, Y., Euskirchen, G., Ward, W.W. and Prasher, D.C. (1994) Green fluorescent protein as a marker for gene expression. Science 263, 802-805.
15. Heim, R., Cubitt, A.B. and Tsien, R.Y. (1995) Improved green fluorescence. Nature 373, 663-664.
16. Flach, J., Bossie, M., Vogel, J., Corbett, A., Jinks, T., Willins, D.A. and Silver, P.A. (1994) A yeast RNA-binding protein shuttles between the nucleus and the cytoplasm. Mol. Cell. Biol. 14,8399-8407.
17. Pines, J. (1995) GFP in mammalian cells. Trends Genet. 11, 326-327.
18. Brown, T.R., Lubahn, D.B., Wilson, E.M., French, F.S., Migeon, C.J. and Corden, J.L. (1990) Functional characterisation of naturally occurring mutant androgen receptors from subjects with complete androgen insensitivity. Mol. Endocrinol. 4, 1759-1772.
19. Lobaccaro, J.M., Lumbroso, J.M., Poujol, N., Georget, V., Brinkmann, A.O., Malpuech, G. and Sultan, C. (1995) Complete androgen insensitivity syndrome due to a new frameshift deletion in exon 4 of the androgen receptor gene: functional analysis of the mutant receptor. Mol. Cell. Endocrinol. 111, 21-28.
20. van Laar, J.H., Voorhost-Ogink, M.M., Zegers, N.D., Boersma, W.J.A., Claassen, E., van der Korput, J.A.G.M., Ruizeveld de Winter, J.A., Van der Kwast, T.H., Mulder, E., Trapman, J. and Brinkmann, A.O. (1989) Characterization of polyclonal antibodies against the N-terminal domain of the human androgen receptor. Mol. Cell. Endocrinol. 67, 29-38.
21. Ogawa, H., Inouye, S., Tsuji, F.I., Yasuda, K. and Umesono, K. (1995) Localization, trafficking, and temperature-dependence of the aequorea green fluorescent protein in cultured vertebrate cells. Proc. Natl. Acad. Sci. USA 92, 11899-11903.
22. Jenster, G., van der Korput, H.A.G.M., van Vroonhoven, C., van der Kwast, T.H. and Brinkmann, A.O. (1991) Domains of the human androgen receptor involved in steroid binding, transcriptional activation, and subcellular localization. Mol. Endocrinol. 5, 1396-1404.
23. Jenster, G., Vanderkorput, H.A.G.M., Trapman, J. and Brinkmann, A.O. (1995) Identification of two transcription activation units in the n-terminal domain of the human androgen receptor. J. Biol. Chem. 270, 7341-7346.
24. Htun, H., Barsony, J., Renyi, I., Gould, D.L. and Hager, G.L. (1996) Visualization of glucocorticoid receptor translocation and intranuclear organization in living cells with a green fluorescent protein chimera. Proc. Natl. Acad. Sci. USA 93, 4845-4850.
25. Carey, K.L., Richards, S.A., Lounsbury, K.M. and Macara, I.G. (1996) Evidence using a green fluorescent protein-glucocorticoid receptor chimera that the RAN/TC4 GTPase mediates an essential function independent of nuclear protein import. J. Cell Biol. 133, 985-996.
26. Guiochon-Mantel, A., Delabre, K., Lescop, P. and Milgrom, E. (1994) Nuclear localization signals also mediate the outward movement of proteins from the nucleus. Proc. Natl. Acad. Sci. USA 91, 7179-7183.