A lineage-selective knockout establishes the critical role of transcription factor GATA-1 in megakaryocyte growth and platelet development

EMBO Journal

Volumn 16, Issue 13, 1997, Pages 3965-3973

  Shivdasani, Ramesh A ^a,b,c,d   Fujiwara, Yuko ^b,d,e   McDevitt, Michael A ^b,c,d,e   Orkin, Stuart H ^a,b,d,e  

^a DANA FARBER CANCER INSTITUTE   (United States)

^b BOSTON CHILDREN S HOSPITAL   (United States)

^c BRIGHAM AND WOMEN S HOSPITAL   (United States)

^d HARVARD MEDICAL SCHOOL   (United States)

^e HOWARD HUGHES MEDICAL INSTITUTE   (United States)

Author keywords

Conditional gene inactivation; Differentiation; GATA transcription factors; Megakaryocytes; Platelets

Indexed keywords

RECOMBINASE; TRANSCRIPTION FACTOR; TRANSCRIPTION FACTOR GATA 1;

ANEMIA; ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; CELL GROWTH; CELL LINEAGE; CELL MATURATION; CELL PROLIFERATION; CELL ULTRASTRUCTURE; CONTROLLED STUDY; CYTOPLASM; EMBRYO DEATH; EMBRYO DEVELOPMENT; EOSINOPHIL; FEMALE; GENE FUNCTION; GENE LOCUS; GENE MUTATION; GENE STRUCTURE; MALE; MAST CELL; MEGAKARYOCYTE; MOUSE; MOUSE MUTANT; MOUSE STRAIN; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; SERTOLI CELL; THROMBOCYTE; THROMBOCYTE COUNT; TRANSGENE;

ANIMALS; BLOOD PLATELETS; CELL DIVISION; CELL LINEAGE; DNA-BINDING PROTEINS; ERYTHROID-SPECIFIC DNA-BINDING FACTORS; FEMALE; GATA1 TRANSCRIPTION FACTOR; GENE EXPRESSION REGULATION; GENE TARGETING; MALE; MEGAKARYOCYTES; MICE; MICE, KNOCKOUT; MUTAGENESIS; NEOMYCIN; TRANSCRIPTION FACTORS;

EID: 0030926006     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1093/emboj/16.13.3965     Document Type: Article

References (45)

1. Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D.D., Smith, J.A., Seidman, J.G. and Struhl, K. (1987) Current Protocols in Molecular Biology. Wiley, New York.
2. Briegel, K., Lim, K.-C., Plank, C., Beug, H., Engel, J. and Zenke, M. (1993) Ectopic expression of a conditional GATA-2/estrogen receptor chimera arrests erythroid differentiation in a hormone-dependent manner. Genes Dev., 7, 1097-1109.
3. Cairns, L.A. et al. (1994) Immortalization of multipotent growth-factor dependent hemopoietic progenitors from mice transgenic for GATA-1 driven SV40 tsA58 gene. EMBO J., 13, 4577-4586.
4. Capecchi, M.R. (1989) Altering the genome by homologous recombination. Science, 244, 1288-1292.
5. Chomczynski, P. and Sacchi, N. (1987) Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem., 162, 156-159.
6. Feil, R., Brocard, J., Mascrez, B., LeMeur, M., Metzger, D. and Chambon, P. (1996) Ligand-activated site-specific recombination in mice. Proc. Natl Acad. Sci. USA, 93, 10887-10890.
7. Ferradini, L., Gu, H., De Smet, A., Rajewsky, K., Reynaud, C.-A. and Weill, J.-C. (1996) Rearrangement-enhancing element upstream of the mouse immunoglobulin kappa chain J cluster. Science, 271, 1416-1420.
8. Fiering, S. et al. (1995) Targeted deletion of 5′HS2 of the murine β-globin LCR reveals that it is not essential for proper regulation of the β-globin locus. Genes Dev., 9, 2203-2213.
9. Fujiwara, Y., Browne, C.P. Cunniff, K., Goff, S.C. and Orkin, S.H. (1996) Arrested development of embryonic red cell precursors in mouse embryos lacking transcription factor GATA-1. Proc. Natl Acad. Sci. USA, 93, 12355-12358.
10. Grewal, T., Theisen, M., Borgmeyer, U., Grusenmeyer, T., Rupp, R.A., Stief, A., Qian, F., Hecht, A. and Sippel, A.E. (1992) The -6.1-kilobase chicken lysozyme enhancer is a multifactorial complex containing several cell-type-specific elements. Mol. Cell Biol., 12, 2339-2350.
11. Grupe, A., Hultgren, B., Ryan, A., Ma, Y.H., Bauer, M. and Stewart, T.A. (1995) Transgenic knockouts reveal a critical requirement for pancreatic β cell glucokinase in maintaining glucose homeostasis. Cell, 83, 69-78.
12. Gu, H., Marth, J.D., Orban, P.C., Mossmann, H. and Rajewsky, K. (1994) Deletion of a DNA polymerase β gene segment in T cells using cell type-specific gene targeting. Science, 265, 103-106.
13. Hug, B.A., Wesselschmidt, R.L., Fiering, S., Bender, M.A., Epner, E., Groudine, M. and Ley, T.J. (1996) Analysis of mice containing a targeted deletion of β-globin locus control region 5′ hypersensitive site 3. Mol. Cell Biol., 16, 2906-2912.
14. Ito, E., Toki, T., Ishihara, H., Ohtani, H., Gu, L., Yokoyama, M., Engel, J.D. and Yamamoto, M. (1993) Erythroid transcription factor GATA-1 is abundantly transcribed in mouse testis. Nature, 362, 466-468.
15. Jackson, H., Williams, N., Bertoncello, J. and Green, R. (1994) Classes of primitive murine megakaryocytic progenitor cells. Exp. Hematol., 22, 954-958.
16. Kuhn, R., Schwenk, E, Aguet, M. and Rajewsky, K. (1995) Inducible gene targeting in mice. Science, 269, 1427-1429.
17. Kulessa, H., Frampton, J. and Graf, T. (1995) GATA-1 reprograms avian myelomonocytic cell lines into eosinophils, thromboblasts, and erythroblasts. Genes Dev., 9, 1250-1262.
18. Lemarchandel, V., Ghysdael, J., Mignotte, V., Rahuel, C. and Romeo, P.-H. (1993) GATA and Ets cis-acting sequences mediate megakaryocyte-specific expression. Mol. Cell Biol., 13, 668-676.
19. Leroy-Viard, K., Vinit, M.-A., Lecointe, N., Mathieu-Mahul, D. and Romeo, P.-H. (1994) Distinct DNase-I hypersensitive sites are associated with TAL-1 transcription in erythroid and T-cell lines. Blood, 84, 3819-3827.
20. Li, E., Bestor, T.H. and Jaenisch, R. (1992) Targeted mutation of the DNA methyltransferase gene results in embryonic lethality. Cell, 69, 915-926.
21. Long, M.W., Gragowski, L.L., Heffner, C.H. and Boxer, L.A. (1985) Phorbol diesters stimulate the development of an early murine progenitor cell. The burst-forming unit-megakaryocyte. J. Clin. Invest., 76, 431-438.
22. May, G. and Enver, T. (1995) Targeting gene expression to haemopoietic stem cells: a chromatin-dependent upstream element mediates cell type-specific expression of the stem cell antigen CD34. EMBO J., 14, 564-574.
23. McDevitt, M.A., Fujiwara, Y., Shivdasani, R.A. and Orkin, S.H. (1997a) An upstream DNase I hypersensitive region of the hematopoietic-expressed transcription factor GATA-1 gene confers developmental specificity in transgenic mice. Proc. Natl Acad. Sci. USA, in press.
24. McDevitt, M.A., Shivdasani, R.A., Fujiwara, Y., Yang, H. and Orkin, S.H. (1997b) A 'knockdown' mutation created by cis-element gene targeting reveals the dependence of red blood cell maturation on the level of transcription factor GATA-1. Proc. Natl Acad. Sci. USA, in press.
25. McDonald, T.P. and Jackson, C.W. (1990) Thrombopoietin derived from human embryonic kidney cells stimulates an increase in DNA content of murine megakaryocytes in vivo. Exp. Hematol., 18, 758-763.
26. Metzger, D., Clifford, J., Chiba, H. and Chambon, P. (1995) Conditional site-specific recombination in mammalian cells using a ligand-dependent chimeric Cre recombinase. Proc. Natl Acad. Sci. USA, 92, 6991-6995.
27. O'Gorman, S., Fox, D.T. and Wahl, G. (1991) Recombinase-mediated gene activation and site-specific integration in mammalian cells. Science, 251, 1351-1355.
28. Olson, E.N., Amold, H.-H., Rigby, P.W.J. and Wold, B.J. (1996) Know your neighbors: three phenotypes in null mutants of the myogenic bHLH gene MRF4. Cell, 85, 1-4.
29. Pevny, L., Simon, M.C., Robertson, E., Klein, W.H., Tsai, S.-F., D'Agati, V. Orkin, S.H. and Costantini, F. (1991) Erythroid differentiation in chimeric mice blocked by a targeted mutation in the gene for transcription factor GATA-1. Nature, 349, 257-260.
30. Pevny, L., Lin, C.-S., D'Agati, V., Simon, M.C., Orkin, S.H. and Costantini, F. (1995) Development of hematopoietic cells lacking transcription factor GATA-1. Development, 121, 163-172.
31. Sauer, B. and Henderson, N. (1990) Targeted insertion of exogenous DNA into the eukaryotic genome by the Cre recombinase. New Biologist, 2, 441-449.
32. Semenza, G.L., Koury, S.T. Nejfelt, M.K., Gearhart, J.D. and Antonarakis, S.E. (1991) Cell-type-specific and hypoxia-inducible expression of the human erythropoietin gene in transgenic mice. Proc. Natl Acad. Sci. USA, 88, 8725-8729.
33. Shivdasani, R.A. (1997) Transcription factors in megakaryocyte differentiation and gene expression. In Kuter, D.J., Hunt, P. Sheridan, W. and Zucker-Franklin, D. (eds). Thrombopoiesis and Thrombopoietins. Humana Press, Totowa, NJ, pp. 189-202.
34. Shivdasani, R.A., Mayer, E.L. and Orkin, S.H. (1995a) Absence of blood formation in mice lacking the T-cell leukemia oncoprotein tal-1/SCL. Nature, 373, 432-434.
35. Shivdasani, R.A., Rosenblatt, M.F., Zucker-Franklin, D., Jackson, C.W., Hunt, P., Saris, C. and Orkin, S.H. (1995b) Transcription factor NF-E2 is required for platelet formation independent of the actions of thrombopoietin/MGDF in megakaryocyte development. Cell, 81, 695-704.
36. Tsien, J.Z., Chen, D.K, Gerber, D., Tom, C., Mercer, E.H., Anderson, D.J., Mayford, M., Kandel, E.R. and Tonegawa, S. (1996) Subregion- and cell type-restricted gene knockout in mouse brain. Cell, 87, 1317-1326.
37. Vidal, M., Morris, R. and Grosveld, F. (1990) Tissue-specific control elements of the Thy-1 gene. EMBO J., 9, 833-840.
38. Visvader, J. and Adams, J.M. (1993) Megakaryocytic differentiation induced in 416B myeloid cells by GATA-2 and GATA-3 transgenes or 5-azacytidine is tightly coupled to GATA-1 expression. Blood, 82, 1493-1501.
39. Visvader, J.E., Elefanty, A.G., Strasser, A. and Adams, J.M. (1992) GATA-1 but not SCL induces megakaryocytic differentiation in an early myeloid line. EMBO J., 11, 4557-4564.
40. Weiss, M.J. and Orkin, S.H. (1995) Transcription factor GATA-1 permits survival and maturation of erythroid precursors by preventing apoptosis. Proc. Natl Acad. Sci. USA, 92, 9623-9627.
41. - embryonic stem cells. Genes Dev., 8, 1184-1197.
42. Weiss, M.J., Yu, C. and Orkin, S.H. (1997) Erythroid-specific properties of transcription factor GATA-1 revealed by phenotypic rescue of a gene-targeted cell line. Mol. Cell Biol., 17, 1642-1651.
43. Wong, P.M.C., Chung, S.W., Chui, D.H.K. and Eaves, C.J. (1986) Properties of the earliest clonogenic hematopoietic precursors to appear in the developing murine yolk sac. Proc. Natl Acad. Sci. USA, 83, 3851-3854.
44. Yomogida, K., Ohtani, H., Harigae, H., Ito, E., Nishimune, Y., Engel, J.D. and Yamamoto, M. (1994) Developmental stage- and spermatogenic cycle-specific expression of transcription factor GATA-1 in mouse Sertoli cells. Development, 120, 1759-1766.
45. Zucker-Franklin, D. (1989) Megakaryocytes and platelets. In Zucker-Franklin, D., Greaves, M.F., Grossi, C.E. and Marmont, A.M. (eds). Atlas of Blood Cells: Function and Pathology. Lea & Febiger, Philadelphia, PA, pp. 559-602.