메뉴 건너뛰기




Volumn 276, Issue 5312, 1997, Pages 589-592

Control of inflammation, cytokine expression, and germinal center formation by BCL-6

Author keywords

[No Author keywords available]

Indexed keywords

DNA; TRANSCRIPTION FACTOR;

EID: 0030901289     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.276.5312.589     Document Type: Article
Times cited : (801)

References (51)
  • 1
    • 0027495491 scopus 로고
    • B. H. Ye et al., Science 262, 747 (1993); A. Migliazza et al., Proc. Natl. Acad. Sci. U.S.A. 92, 12520 (1995); T. Miki, N. Kawamata, S. Hirosawa, N. Aoki, Blood 83, 26 (1994); J. P. Kerckaert et al., Nature Genet. 5, 66 (1993); B. W. Baron et al., Proc. Natl. Acad. Sci. U.S.A. 90, 5262 (1993); F. LoCoco et al., Blood 83, 1757 (1994); T. Otsuki et al., ibid. 85, 2877 (1995); C. Bastard et al., ibid. 83, 2423 (1994).
    • (1993) Science , vol.262 , pp. 747
    • Ye, B.H.1
  • 2
    • 0029614378 scopus 로고
    • B. H. Ye et al., Science 262, 747 (1993); A. Migliazza et al., Proc. Natl. Acad. Sci. U.S.A. 92, 12520 (1995); T. Miki, N. Kawamata, S. Hirosawa, N. Aoki, Blood 83, 26 (1994); J. P. Kerckaert et al., Nature Genet. 5, 66 (1993); B. W. Baron et al., Proc. Natl. Acad. Sci. U.S.A. 90, 5262 (1993); F. LoCoco et al., Blood 83, 1757 (1994); T. Otsuki et al., ibid. 85, 2877 (1995); C. Bastard et al., ibid. 83, 2423 (1994).
    • (1995) Proc. Natl. Acad. Sci. U.S.A. , vol.92 , pp. 12520
    • Migliazza, A.1
  • 3
    • 0028031988 scopus 로고
    • B. H. Ye et al., Science 262, 747 (1993); A. Migliazza et al., Proc. Natl. Acad. Sci. U.S.A. 92, 12520 (1995); T. Miki, N. Kawamata, S. Hirosawa, N. Aoki, Blood 83, 26 (1994); J. P. Kerckaert et al., Nature Genet. 5, 66 (1993); B. W. Baron et al., Proc. Natl. Acad. Sci. U.S.A. 90, 5262 (1993); F. LoCoco et al., Blood 83, 1757 (1994); T. Otsuki et al., ibid. 85, 2877 (1995); C. Bastard et al., ibid. 83, 2423 (1994).
    • (1994) Blood , vol.83 , pp. 26
    • Miki, T.1    Kawamata, N.2    Hirosawa, S.3    Aoki, N.4
  • 4
    • 0027306091 scopus 로고
    • B. H. Ye et al., Science 262, 747 (1993); A. Migliazza et al., Proc. Natl. Acad. Sci. U.S.A. 92, 12520 (1995); T. Miki, N. Kawamata, S. Hirosawa, N. Aoki, Blood 83, 26 (1994); J. P. Kerckaert et al., Nature Genet. 5, 66 (1993); B. W. Baron et al., Proc. Natl. Acad. Sci. U.S.A. 90, 5262 (1993); F. LoCoco et al., Blood 83, 1757 (1994); T. Otsuki et al., ibid. 85, 2877 (1995); C. Bastard et al., ibid. 83, 2423 (1994).
    • (1993) Nature Genet. , vol.5 , pp. 66
    • Kerckaert, J.P.1
  • 5
    • 0027288335 scopus 로고
    • B. H. Ye et al., Science 262, 747 (1993); A. Migliazza et al., Proc. Natl. Acad. Sci. U.S.A. 92, 12520 (1995); T. Miki, N. Kawamata, S. Hirosawa, N. Aoki, Blood 83, 26 (1994); J. P. Kerckaert et al., Nature Genet. 5, 66 (1993); B. W. Baron et al., Proc. Natl. Acad. Sci. U.S.A. 90, 5262 (1993); F. LoCoco et al., Blood 83, 1757 (1994); T. Otsuki et al., ibid. 85, 2877 (1995); C. Bastard et al., ibid. 83, 2423 (1994).
    • (1993) Proc. Natl. Acad. Sci. U.S.A. , vol.90 , pp. 5262
    • Baron, B.W.1
  • 6
    • 0028230387 scopus 로고
    • B. H. Ye et al., Science 262, 747 (1993); A. Migliazza et al., Proc. Natl. Acad. Sci. U.S.A. 92, 12520 (1995); T. Miki, N. Kawamata, S. Hirosawa, N. Aoki, Blood 83, 26 (1994); J. P. Kerckaert et al., Nature Genet. 5, 66 (1993); B. W. Baron et al., Proc. Natl. Acad. Sci. U.S.A. 90, 5262 (1993); F. LoCoco et al., Blood 83, 1757 (1994); T. Otsuki et al., ibid. 85, 2877 (1995); C. Bastard et al., ibid. 83, 2423 (1994).
    • (1994) Blood , vol.83 , pp. 1757
    • LoCoco, F.1
  • 7
    • 0029024622 scopus 로고
    • B. H. Ye et al., Science 262, 747 (1993); A. Migliazza et al., Proc. Natl. Acad. Sci. U.S.A. 92, 12520 (1995); T. Miki, N. Kawamata, S. Hirosawa, N. Aoki, Blood 83, 26 (1994); J. P. Kerckaert et al., Nature Genet. 5, 66 (1993); B. W. Baron et al., Proc. Natl. Acad. Sci. U.S.A. 90, 5262 (1993); F. LoCoco et al., Blood 83, 1757 (1994); T. Otsuki et al., ibid. 85, 2877 (1995); C. Bastard et al., ibid. 83, 2423 (1994).
    • (1995) Blood , vol.85 , pp. 2877
    • Otsuki, T.1
  • 8
    • 0028331006 scopus 로고
    • B. H. Ye et al., Science 262, 747 (1993); A. Migliazza et al., Proc. Natl. Acad. Sci. U.S.A. 92, 12520 (1995); T. Miki, N. Kawamata, S. Hirosawa, N. Aoki, Blood 83, 26 (1994); J. P. Kerckaert et al., Nature Genet. 5, 66 (1993); B. W. Baron et al., Proc. Natl. Acad. Sci. U.S.A. 90, 5262 (1993); F. LoCoco et al., Blood 83, 1757 (1994); T. Otsuki et al., ibid. 85, 2877 (1995); C. Bastard et al., ibid. 83, 2423 (1994).
    • (1994) Blood , vol.83 , pp. 2423
    • Bastard, C.1
  • 9
    • 0029019660 scopus 로고
    • T. Onizuka et al., Blood 86, 28 (1995).
    • (1995) Blood , vol.86 , pp. 28
    • Onizuka, T.1
  • 11
  • 12
    • 0029904294 scopus 로고    scopus 로고
    • D. Allman et al., ibid. 87, 5257 (1996).
    • (1996) Blood , vol.87 , pp. 5257
    • Allman, D.1
  • 14
    • 0029416999 scopus 로고
    • P. Dhordain et al., ibid. 11, 2689 (1995); C. Deweindt et al., Cell Growth Differ. 6, 1495 (1995).
    • (1995) Oncogene , vol.11 , pp. 2689
    • Dhordain, P.1
  • 15
    • 0028822226 scopus 로고
    • P. Dhordain et al., ibid. 11, 2689 (1995); C. Deweindt et al., Cell Growth Differ. 6, 1495 (1995).
    • (1995) Cell Growth Differ. , vol.6 , pp. 1495
    • Deweindt, C.1
  • 18
    • 1842415687 scopus 로고    scopus 로고
    • note
    • -/- mice used in these experiments resulted from intercrosses between the 129 strain from which the ES cells were derived and strain C57B1/6.
  • 19
    • 1842325892 scopus 로고    scopus 로고
    • note
    • Mice were immunized intraperitoneally with 100 μg of TNP-KLH in complete Freund's adjuvant (CFA). After 12 days, splenocytes were lysed and immunoprecipitated with the indicated antibodies. Immunoprecipitates were electrophoresed through a 7.5% SDS-polyacrylamide gel electrophoresis gel, transferred to nitrocellulose, incubated, and developed with a rabbit antibody to BCL-6 (5), with the use of the ECL system (Amersham).
  • 20
    • 1842404443 scopus 로고    scopus 로고
    • note
    • Bone marrow cells, splenocytes, and thymocytes were analyzed by flow cytometry for the following surface proteins: CD4, CD8, CD45R (B220), IgM, CD3, CD24 (HSA), CD43, CD11b (Mac1), Gr-1, CD90 (Thy-1), CD5, IgD, TCR-αβ, CD25, CD23, CD38, CD80 (B7.1), CD86 (B7.2), MHC class II (IA), CD11a (LFA-1), CD19, CD18, CD40 ligand, CTLA-4, and CD-69. No differences between BCL-6 mutant mice and wild-type littermate control mice were observed.
  • 21
    • 1842403867 scopus 로고    scopus 로고
    • note
    • Mice 5 to 6 weeks old were immunized intraperitoneally with 100 μg of TNP-KLH in CFA. After 12 days, serum was collected for anti-TNP titers, and spleens were processed for immunohistochemistry by being embedded in OCT compound (Tissue-Tek) and frozen. Splenic cryosections were stained with PNA-horseradish peroxidase, biotin-anti-mouse IgD, and streptavidin-alkaline phosphatase according to standard procedures (15). For T cell-independent immunization, mice were injected intraperitoneally with 25 μg of TNP-conjugated Ficoll suspended in phosphate-buffered saline. Serum was collected for anti-TNP titers after 7 days. Anti-TNP titers were assayed from sera by ELISA on TNP-ovalbumin-coated plates with the use of alkaline phosphatase-conjugated, goat anti-mouse Ig antibodies specific for various Ig isotypes (Southern Biotech).
  • 24
    • 1842365762 scopus 로고    scopus 로고
    • unpublished observations
    • A. Dent, unpublished observations.
    • Dent, A.1
  • 25
    • 1842293025 scopus 로고    scopus 로고
    • note
    • 6 per milliliter on anti-CD3 coated plates for 24 hours before collection of supernatants. ELISA antibodies and standards were obtained from Pharmingen (IFN-γ and IL-4), A. Sher (IL-5), and R&D Systems (IL-13). To prepare lung and heart inflammatory cells, tissues were minced, ground between glass slides, and filtered through a 70-μm nylon mesh.
  • 27
    • 0028201726 scopus 로고
    • R. A. Seder, Annu. Rev. Immunol. 12, 635 (1994); A. K. Abbas, K. M. Murphy, A. Sher, Nature 383, 787 (1996).
    • (1994) Annu. Rev. Immunol. , vol.12 , pp. 635
    • Seder, R.A.1
  • 28
  • 29
    • 15844404509 scopus 로고    scopus 로고
    • K. Takeda et al., Nature 380, 627 (1996); K. Shimoda et al., ibid., p. 630; M. H. Kaplan, U. Schindler, S. T. Smiley, M. J. Grusby, Immunity 4, 313 (1996).
    • (1996) Nature , vol.380 , pp. 627
    • Takeda, K.1
  • 30
    • 15844404509 scopus 로고    scopus 로고
    • K. Takeda et al., Nature 380, 627 (1996); K. Shimoda et al., ibid., p. 630; M. H. Kaplan, U. Schindler, S. T. Smiley, M. J. Grusby, Immunity 4, 313 (1996).
    • Nature , pp. 630
    • Shimoda, K.1
  • 34
    • 0029003128 scopus 로고
    • N. Kawamata et al., Biochem. Biophys. Res. Commun. 204, 366 (1994); B. W. Baron et al., Genes Chromosomes Cancer 13, 221 (1995).
    • (1995) Genes Chromosomes Cancer , vol.13 , pp. 221
    • Baron, B.W.1
  • 35
    • 1842298994 scopus 로고    scopus 로고
    • note
    • 3-4GAA.
  • 36
    • 1842374750 scopus 로고    scopus 로고
    • note
    • Etectrophoretic mobility-shift assay (EMSA) DNA binding reactions were performed essentially as described (6). The CD23b DMA probe sequence is 5′-GATCAGGGTGAATTTCTAAGAAAGGGACTGGTGT-3′. BCL-6 protein and the luciferase control protein were generated with in vitro translation as described (6). Nuclear extracts were prepared essentially as described (35). For antibody supershift analysis, 3 μg of antibody [anti-BCL-6 (N3) or anti-State (S20); Santa Cruz Biotechnology] or preimmune rabbit serum was used.
  • 38
    • 1842295418 scopus 로고
    • J. Hou et al., Science 265 (1994); H. Kotanides and N. C. Reich, ibid. 262, 1265 (1993); C. Schindler, H. Kashleva, A. Pernis, R. Pine, P. Rothman, EMBO J. 13, 1350 (1994).
    • (1994) Science , vol.265
    • Hou, J.1
  • 39
    • 0027688778 scopus 로고
    • J. Hou et al., Science 265 (1994); H. Kotanides and N. C. Reich, ibid. 262, 1265 (1993); C. Schindler, H. Kashleva, A. Pernis, R. Pine, P. Rothman, EMBO J. 13, 1350 (1994).
    • (1993) Science , vol.262 , pp. 1265
    • Kotanides, H.1    Reich, N.C.2
  • 41
    • 1842403228 scopus 로고    scopus 로고
    • note
    • A CD23b luciferase reporter construct was prepared by tandem ligation of three human CD23b BCL-6/Stat6 sites (24) immediately 5′ to a minimal TK promoter (base pairs -81 to +52) in pGL2basic (Promega). NIH 3T3 cells were transfected by the calcium phosphate transfection method using 2 μg of β-galactosidase (β-Gal) expression vector (pSV2Bgal), 5 μg of CD23b reporter plasmid, 10 μg of Stat6 expression plasmid (in the vector pCEV27) or empty pCEV27 vector, and 10 μg of BCL-6 expression vector [pCGN-BCL-6 (6)] or empty pCGN vector control. The day after transfection, cells were incubated for 16 hours in serum-free medium and then treated with 500 U/ml (0.25 ng/ml) of mouse IL-4 for 6 hours. Cell extracts were prepared and analyzed with the Luciferase Assay System (Promega). Luciferase activity was normalized on the basis of β-Gal activity in each extract.
  • 42
    • 1842376568 scopus 로고    scopus 로고
    • note
    • 6 WI-L2-NS cells were transfected with 6 μg of marker expression vector for mouse intercellular adhesion molecule-1 (ICAM) (pmICAM) and 30 μg of expression vector for BCL-6 [pCGN BCL-6 (6)] or empty vector (pCGN) using a BTX electroporator set at 260 V, 950 mF, and 720 ohms. After overnight culture, the transfected cells were stimulated with recombinant human IL-4 (10 ng/ml; Genzyme) for 16 hours and subsequently stained for flow cytometry with mouse anti-human CD23-PE (M-L233, Pharmingen) and hamster anti-mouse CD54/ICAM (3E2, Pharmingen).
  • 44
    • 0029991923 scopus 로고    scopus 로고
    • M. Matsumoto et al., Science 271, 1289 (1996); M. Pasparakis, L. Alexopoulou, V. Episkopou, G. Kollias, J. Exp. Med. 184, 1397 (1996).
    • (1996) Science , vol.271 , pp. 1289
    • Matsumoto, M.1
  • 46
    • 1842291812 scopus 로고    scopus 로고
    • note
    • -/- and wild-type lymphocytes were comparable.
  • 47
    • 1842288862 scopus 로고    scopus 로고
    • note
    • -/- mice (n = 5) had, on average, 3.1 μg/ml of serum IgE after immunization with TNP-KLH in CFA, whereas wild-type littermates (n = 6) had 0.36 μg/ml of serum IgE.
  • 48
    • 0027452977 scopus 로고
    • M. Kopf et al., Nature 362, 245 (1993).
    • (1993) Nature , vol.362 , pp. 245
    • Kopf, M.1
  • 51
    • 1842293635 scopus 로고    scopus 로고
    • note
    • -/- mice; J. Inman for TNP-Ficoll; W. LaRochelle for the State expression vector; P. Caspar and A. Sher for help with the anti-IL-5 ELISA and helpful discussions; B. Paul for recombinant mouse IL-4 and helpful discussions; K. Kelly for the pmICAM expression plasmid; and T. Waldmann, R. Germain, R. Hodes, and H. Morse for helpful discussions. Mice were maintained according to the NIH Office of Animal Care and Use guidelines.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.