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Mice were immunized with SRBCs, and frozen sections of spleen were prepared and stained as described in Fig 1 The rat monoclonal antibody MOMA-1 (16) (Serotec) was detected with goat antibody to rat IgG conjugated with biotin (Southern Biotechnology) and HRP-streptavidin (Zymed).
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-/- bone marrow cells, respectively. Ten weeks after bone marrow transfer, mice were immunized with SRBCs. Ten days later, spleen sections were stained with PNA and antibody to IgD as shown in Fig 1.
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Animal experiments were conducted in accordance with institutional guidelines for Washington University School of Medicine.
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We thank J. Cyster for discussions regarding MOMA-1 staining, O Kanagawa and R. Schreiber for critical reading of the manuscript, and B. Wnght and G Huang for assistance with histological evaluations. This work was supported in part by grants from NIH (D.D.C.) and the Monsanto Company (M.H.N.). D.D.C. is an investigator of the Howard Hughes Medical Institute.
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