Baculovirus expression provides direct evidence for heteromeric assembly of P2X2 and P2X3 receptors

Journal of Neuroscience

Volumn 17, Issue 17, 1997, Pages 6529-6533

  Radford, Kathryn M ^a   Virginio, Caterina ^a   Surprenant, Annmarie ^a   North, R Alan ^a   Kawashima, Eric ^a  

^a GLAXOSMITHKLINE   (United States)

Author keywords

ATP; Baculovirus; Cation channels; Coimmunoprecipitation; P2X receptors; Subunit assembly

Indexed keywords

ADENOSINE TRIPHOSPHATE; CATION CHANNEL; PURINE P2X RECEPTOR; RECEPTOR SUBUNIT;

ANIMAL CELL; ARTICLE; BACULOVIRUS; CONTROLLED STUDY; INSECT; MOLECULAR DYNAMICS; MOLECULAR INTERACTION; NONHUMAN; PRIORITY JOURNAL; PROTEIN ASSEMBLY;

EID: 0030879448     PISSN: 02706474     EISSN: None     Source Type: Journal    
DOI: 10.1523/jneurosci.17-17-06529.1997     Document Type: Article

References (20)

1. Barnard EA (1996) The transmitter-gated channels: a range of receptor types and structures. Trends Pharmacol Sci 17:305-309.
2. 1 receptor activation in HL60 cells. Blood 87:2659-2664.
3. 6 receptors and the distribution and properties of an extended family of ATP-gated ion channels. J Neurosci 16:2495-2507.
4. Cook SP, Vulchanova L, Hargreaves KM, Elde RP, McCleskey EW (1997) Distinct P2X receptors mediate ATP actions on nociceptive and non-nociceptive neurons. Nature 387:505-508.
5. 2X purinoceptors). Mol Pharmacol 48:178-183.
6. Evans RJ, Lewis C, Virginio C, Lundstrom K, Buell G, Surprenant A, North R A (1996) Ionic permeability of, and divalent cation effects on, two ATP-gated cation channels (P2X receptors) expressed in mammalian cells. J Physiol (Lond) 497:413-422.
7. Grussenmeyer T, Scheidtmann KH, Hutchinson MA, Eckhart W, Walter G (1985) Complexes of polyoma virus medium T antigen and cellular proteins. Proc Natl Acad Sci USA 82:7952-7954.
8. 2X purinoceptors in rat superior cervical, nodose, and guinea pig coeliac neurons. J Physiol (Lond) 484:385-395.
9. Knudson DL, Tinsley TW (1974) Replication of a nuclear polyhedrosis virus in a continuous cell culture of Spodoptera frugiperda: purification, assay of infectivity, and growth characteristics of the virus. J Virol 14:934-944.
10. 3 receptor subunits can account for ATP-gated currents in sensory neurons. Nature 377:432-435.
11. 2 purinoceptors. Br J Pharmacol 118:1806-1812.
12. 33P]-ATP. Br J Pharmacol 117:1254-1260.
13. 35S]ATP-γS. Br J Pharmacol 118:18P.
14. Nielsen LK, Smyth GK, Greenfield PF (1991) Hemocytometer cell count distributions: implications of non-Poisson behaviour. Biotechnol Prog 7:560-563.
15. Nielsen LK, Smyth GK, Greenfield PF (1992) Accuracy of the endpoint assay for virus titration. Cytotechnology 8:231-236.
16. North RA (1996) Families of ion channels with two hydrophobic segments. Curr Opin Cell Biol 8:474-483.
17. O'Reilly DR, Miller LK, Luckow VA (1994) An overview of baculoviruses. In: Baculovirus expression vectors: a laboratory manual (O'Reilly DR, Miller LK, Luckow VA, eds), pp 8-10. New York: Freeman.
18. 2X receptors bring new structure to ligand-gated ion channels. Trends Neurosci 18:224-229.
19. Vulchanova L, Arvidsson U, Riedl M, Wang J, Buell G, Surprenant A, North RA, Elde RP (1996) Differential distribution of two ATP-gated ion channels (P2X receptors) determined by immunocytochemistry. Proc Natl Acad Sci USA 93:8063-8067.
20. Whitehorn EA, Tate E, Yanofsky SD, Kochersperger L, Davis A, Mortensen RB, Yonkovich S, Bell K, Dower WJ, Barrett RW (1995) A generic method for expression and use of "tagged" soluble versions of cell surface receptors. Biotechnology 13:1215-1219.