A comparison of the binding characteristics of recombinant P(2X1) and P(2X2) purinoceptors

British Journal of Pharmacology

Volumn 118, Issue 7, 1996, Pages 1806-1812

  Michel, Anton D ^a   Lundström, Kenneth ^b   Buell, Gary N ^c   Surprenant, Annmarie ^c   Valera, Soledad ^c   Humphrey, Patrick P A ^a  

^a UNIVERSITY OF CAMBRIDGE   (United Kingdom)

^b GLAXOSMITHKLINE   (United Kingdom)

^c GLAXO INST FOR MOLECULAR BIOLOGY   (Switzerland)

Author keywords

ATP; CHO K1 cell; P(2X1) purinoceptor; P(2X2) purinoceptor; PC12 phaeochromocytoma; Rat bladder; Rat vas deferens; Semliki forest virus; 35S adenosine 5' O (3 thiotriphosphate); 3H , methylene

Indexed keywords

4,4' DIISOTHIOCYANATOSTILBENE 2,2' DISULFONIC ACID; ADENOSINE 5' O (3 THIOTRIPHOSPHATE); ALPHA,BETA METHYLENEADENOSINE TRIPHOSPHATE; CALCIUM CHLORIDE; DEOXYADENOSINE TRIPHOSPHATE; DEXTRAN SULFATE; DIVALENT CATION; PURINE P2X RECEPTOR; PURINE P2X1 RECEPTOR; PURINE P2X2 RECEPTOR; PYRIDOXAL PHOSPHATE 6 AZOPHENYL 2',4' DISULFONIC ACID; RECEPTOR SUBUNIT; SULFUR 35; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; BINDING AFFINITY; BINDING SITE; BLADDER; CHO CELL; COMPARATIVE STUDY; CONTROLLED STUDY; ELECTROPHYSIOLOGY; HUMAN; HUMAN CELL; ISOTOPE LABELING; NONHUMAN; PHEOCHROMOCYTOMA CELL; PRIORITY JOURNAL; RAT; RECEPTOR BINDING; SEMLIKI FOREST ALPHAVIRUS; SPECIES DIFFERENCE; VAS DEFERENS;

EID: 0030005332     PISSN: 00071188     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1476-5381.1996.tb15607.x     Document Type: Article

References (27)

1. ABBRACCHIO, M.P. & BURNSTOCK, G. (1994). Purinoceptors: Are there families of P2X and P2Y purinoceptors? Pharmacol. Ther., 64, 445-475.
2. BO, X., ZHANG, Y., NASSAR, M., BURNSTOCK, G. & SCHOEPFER, R. (1995). A P2X purinoceptor cDNA conferring a novel pharmacological profile. FEBS Lett., 375, 129-133.
3. BRAKE, A.J., WAGENBACH, M.J. & JULIUS, D. (1994). New structural motif for ligand-gated ion channels defined by an ionotropic ATP receptor. Nature, 371, 519-523.
4. 2x purinoceptors in rat vas deferons. Br. J. Pharmacol., 112, 690-694.
5. BURNSTOCK, G. & KENNEDY, C. (1985). Is there a basis for distinguishing two types of P2-purinoceptor? Gen. Pharmacol., 16, 433-440.
6. CHEN, C-C., AKOPIAN, A.N., SIVILOTTI, L., COLQUHOUN, D., BURNSTOCK, G. & WOOD, J.N. (1995). A P2X-purinoceptor expressed by a subset of sensory neurons. Nature, 377, 428-431.
7. 50) of an enzymatic reaction. Biochem. Pharmacol., 22, 3099-3108.
8. +. J. Biol. Chem., 269, 14525-14529.
9. 2-purinoceptors in the smooth muscle of the rat tail artery: a comparison between contractile and electrophysiological responses. Br. J. Pharmacol., 113, 853-860.
10. 2x-purinoceptors). Mol. Pharmacol., 48, 178-183.
11. FREDHOLM, B.B., ABBRACCHIO, M.P., BURNSTOCK, G., DALY, J.W., HARDEN, T.K., JACOBSON, K.A., LEFF, P. & WILLIAMS, M. (1994). Nomenclature and classification of purinoceptors. Pharmacol. Rev., 46, 143-156.
12. 2x purinoceptors. Naunyn Schmiedeberg's Arch. Pharmacol., 352, 585-596.
13. INOUE, R. & BRADING, A.F. (1990). The properties of the ATP-induced depolarization and current in single cells isolated from the guinea-pig urinary bladder. Br. J. Pharmacol., 100, 619-625.
14. 2x purinoceptors in rat superior cervical, nodose and guinea-pig coeliac neurones. J. Physiol., 484, 385-395.
15. 2X purinoceptors in rat vas deferens. Eur. J. Pharmacol., 263, 301-309.
16. 2X purinoceptors mediating the electrophysiological and contractile effects of purine nucleotides in rat vas deferens. Br. J. Pharmacol., 115, 177-185.
17. 2X2 and P2X3 receptor can account for ATP-gated currents in sensory neurons. Nature, 377, 432-435.
18. LILJESTRÖM, P. & GAROFF, H. (1991). A new generation of animal cell expression vectors based on the Semliki forest virus replicon. Biotechnology, 9, 1356-1361.
19. LUNDSTRÖM, K., MILLS, A., BUELL, G., ALLET, E., ADAMI, N. & LILJESTRÖM, P. (1994). High-level expression of the human neurokinin-1 receptor in mammalian celllines using the Semliki Forest virus expression system. Eur. J. Biochcm., 224, 917-921.
20. 3H]alpha-beta-methylene ATP binding sites in the rat. Naunyn Schmiedeberg's Arch. Pharmacol., 348, 608 617.
21. 3H]alpha,beta-methylene ATP binding in rat vas deferens. Naunyn Schmiedeberg's Arch. Pharmacol., 350, 113-122.
22. 35S]adenosine 5′-O-[3-thiotriphosphate] in rat vas deferens membranes. Br. J. Pharmacol., 117, 63-70.
23. 33P]ATP. Br. J. Pharmacol., 117, 1254-1260.
24. 2X purinoceptors. Br. J. Pharmacol., 113, 58P.
25. 2X purinoceptors. Naunyn Schmiedeberg's Arch. Pharmacol., 351, 603-609.
26. 2X receptor for extracellular ATP. Nature, 371, 516-519.
27. 2X receptor from urinary bladder. Recept. Channels, 3, 283-289.