The binding characteristics of a human bladder recombinant P2x purinoceptor, labelled with [3H]-αβmeATP, [35S]-ATPγS or [33P]-ATP

British Journal of Pharmacology

Volumn 117, Issue 6, 1996, Pages 1254-1260

  Michel, Anton D ^a   Lundström, Kenneth ^b   Buell, Gary N ^c   Surprenant, Annmarie ^c   Valera, Soledad ^c   Humphrey, Patrick P A ^a  

^a UNIVERSITY OF CAMBRIDGE   (United Kingdom)

^b GLAXOSMITHKLINE   (United Kingdom)

^c GLAXO INST FOR MOLECULAR BIOLOGY   (Switzerland)

Author keywords

CHO K1 cells; Human bladder P2x purinoceptor; Rat vas deferens; Semliki forest virus; 35S ATP S; 3H meATP; 33P ATP

Indexed keywords

ADENOSINE TRIPHOSPHATE; ALPHA,BETA METHYLENEADENOSINE TRIPHOSPHATE; PURINE P2X RECEPTOR; RADIOLIGAND;

ANIMAL CELL; ARTICLE; BINDING AFFINITY; BINDING SITE; CELL MEMBRANE; CHO CELL; NONHUMAN; PRIORITY JOURNAL; RECEPTOR BINDING; SEMLIKI FOREST ALPHAVIRUS; VOLTAGE CLAMP;

ADENOSINE TRIPHOSPHATE; ANIMALS; CELL LINE; CHO CELLS; CRICETINAE; HUMANS; MALE; PATCH-CLAMP TECHNIQUES; PHOSPHORUS RADIOISOTOPES; POLYMERASE CHAIN REACTION; RATS; RATS, SPRAGUE-DAWLEY; RECEPTORS, PURINERGIC P2; RECOMBINANT PROTEINS; SULFUR RADIOISOTOPES; TRITIUM; URINARY BLADDER; VAS DEFERENS;

EID: 0029976229     PISSN: 00071188     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1476-5381.1996.tb16723.x     Document Type: Article

References (24)

1. ABBRACCHIO, M.P. & BURNSTOCK, G. (1994). Purinoceptors: Are there families of P2X and P2Y purinoceptors? Pharmac. Ther., 64, 445-475.
2. BEAN, B.P. (1992). Pharmacology and electrophysiology of ATP-activated ion channels. Trends Pharmacol. Sci., 13, 87-90.
3. 3H]α,β-methylene ATP in rat urinary bladder membranes. Br. J. Pharmacol., 101, 291-196.
4. 2x purinoceptor from rat vas deferens. J. Biol. Chem., 267, 17581-17587.
5. BRAKE, A.J., WAGENBACH, M.J. & JULIUS, D. (1994). New structural motif for ligand-gated ion channels defined by a ionotropic ATP receptor. Nature, 371, 519-523.
6. BURNSTOCK, G. & KENNEDY, C. (1985). Is there a basis for distinguishing two types of P2-purinoceptor? Gen. Pharmacol., 16, 433-440.
7. 2 purinoceptors in the smooth muscle of the rat tail artery: a comparison between contractile and electrophysiological responses. Br. J. Pharmacol., 113, 853-860.
8. 2x-purinoceptors). Mol. Pharmacol., 48, 178-183.
9. FREDHOLM, B.B., ABBRACCHIO, M.P., BURNSTOCK, G., DALY, J.W., HARDEN, T.K., JACOBSON, K.A., LEFF, P. & WILLIAMS, M. (1994). Nomenclature and classification of purinoceptors. Pharmacol. Rev., 46, 143-156.
10. 2-purinoceptor in cultured CHO-K1 cells. Br. J. Pharmacol., 110, 1305-1310.
11. 2x purinoceptors in rat superior cervical, nodose and guinea-pig coeliac neurones. J. Physiol., 484, 385-395.
12. 2x purinoceptors in rat vas deferens. Eur. J. Pharmacol., 263, 301-309.
13. 2x purinoceptors mediating the electrophysiological and contractile effects of purine nucleotides in rat vas deferens. Br. J. Pharmacol., 115, 177-185.
14. LILJESTRÖM, P. & GAROFF, H. (1991). A new generation of animal cell expression vectors based on the Semliki forest virus replicon. Biotechnology, 9, 1356-1361.
15. LUNDSTRÖM, K., MILLS, A., BUELL, G., ALLET, E., ADAMI, N. & LILJESTRÖM, P. (1994). High-level expression of the human neurokinin-1 receptor in mammalian cell lines using the Semliki Forest virus expression system. Eur. J. Biochem., 224, 917-921.
16. 3H]αβmeATP may label an endothelial-derived cell line 5′-nucleotidase with high affinity. Br. J. Pharmacol., 115, 767-774.
17. 3H]α,β-methylene ATP binding sites in the rat. Naunyn Schmied. Arch. Pharmacol., 348, 608-617.
18. 3H]α,β-methylene ATP binding in rat vas deferens. Naunyn Schmied. Arch. Pharmacol., 350, 113-122.
19. 35S]-adenosine 5′-O-[3-thiotriphosphate] in rat vas deferens membranes. Br. J. Pharmacol., 117, 63-70.
20. 2x purinoceptors. Br. J. Pharmacol., 113, 58P.
21. 2 purinoceptor classification. Br. J. Pharmacol., 113, 463-470.
22. 2x receptor for extracellular ATP. Nature, 371, 516-519.
23. 2x receptor from urinary bladder. Recept. Channels, (in press).
24. VOOGD, T.E., VANSTERKENBURG, E.L., WILTING, J & JANSSEN, L.H. (1993). Recent research on the biological activity of suramin. Pharmacol. Rev., 45, 177-203.