New in vitro model of traumatic neuronal injury: Evaluation of secondary injury and glutamate receptor-mediated neurotoxicity

Journal of Neurotrauma

Volumn 14, Issue 9, 1997, Pages 651-663

  Mukhin, A G ^a   Ivanova, S A ^a   Knoblach, S M ^a   Faden, A I ^a  

^a GEORGETOWN UNIVERSITY MEDICAL CENTER   (United States)

Author keywords

Brain trauma; Cell culture; Glutamate antagonists; In vitro model; Neuronal death

Indexed keywords

6 NITRO 7 SULFAMOYLBENZO[F]QUINOXALINE 2,3 DIONE; DIZOCILPINE; GLUTAMATE RECEPTOR; GLUTAMATE RECEPTOR ANTAGONIST; LACTATE DEHYDROGENASE; METABOTROPIC RECEPTOR; N METHYL DEXTRO ASPARTIC ACID;

ANIMAL CELL; ARTICLE; BRAIN INJURY; CELL COUNT; CELL DEATH; EMBRYO; IMMUNOCYTOCHEMISTRY; INJURY SCALE; NERVE CELL CULTURE; NEUROPROTECTION; NEUROTOXICITY; NONHUMAN; RAT; TISSUE CULTURE;

EID: 0030866127     PISSN: 08977151     EISSN: None     Source Type: Journal    
DOI: 10.1089/neu.1997.14.651     Document Type: Article

References (32)

1. AMADOR, E., DORFMAN, L.E., and WACHER, W.E.C. (1963). Serum lactate dehydrogenase activity: an analytical assessment of current assays. Clin. Chem. 9, 391-399.
2. BIRRELL, G.J., and MARCOUX, F.W. (1993). Effect of varying levels of glucose on oxygen deprivation-induced delayed neuronal cell death in primary cerebrocortical cultures. Brain Res. 607, 337-340.
3. BRUNO, V., COPANI, A., BATTAGLIA, G., RAFFAELE, R., SHINOZAKI, H., and NICOLETTI, F. (1994). Protective effect of the metabotropic glutamate receptor agonist, DCG-IV, against excitotoxic neuronal death. Eur. J. Pharm. 256, 109-112.
4. CHENG, B., and MATTSON, M.P. (1995). PDGFs protect hippocampal neurons against energy deprivation and oxidative injury: Evidence for induction of antioxidant pathways. J. Neurosci. 15, 7095-7104.
5. ELLIS, E.F., MCKINNEY, J.S., WILLOUGHBY, K.A., LIANG, S., and POVLISHOCK, J.T. (1995). A new model for rapid stretch-induced injury of cells in culture: characterization of the model using atrocyties. J. Neurotrauma 12, 325-339.
6. FADEN, A.I. (1993). Experimental neurobiology of central nervous system. Crit. Rev. Neurobiol. 7, 175-186.
7. FADEN, A.I., DEMEDIUK, P., PANTER, S.S., and VINK, R. (1989). The role of excitatory amino acids and NMDA receptors in traumatic brain injury. Science 44, 798-800.
8. FADEN, A.I., ELLISON, J.A., and NOBLE, L.J. (1990). Effects of competitive and noncompetitive NMDA receptor antagonists in spinal cord injury. Eur. J. Pharmacol. 175, 165.
9. FADEN, A.I. and SIMON, R.P. (1988). A potential role for excitotoxins in the pathophysiology of spinal cord injury. Ann. Neurol. 23, 623-626.
10. FLAVIN, M.P. (1996). Influence of dexamethsone on neurotoxicity caused by oxygen and glucose deprivation in vitro. Exp. Neurol. 139, 34-38.
11. HAYES, R.L., JENKINS, L.W., LYETH, B.G., et al. (1988). Pretreatment with phencyclidine, an N-methyl-D-aspartate antagonist, attenuates long-term behavioral deficits in the rat produced by traumatic brain injury. J. Neurotrauma 5, 259-274.
12. KEILHOLFF, G., and WOLF, G. (1993). Comparison of double fluorescence staining and LDH-test for monitoring cell viability in vitro. Neuroreport 5, 129-132.
13. KOH, J.Y., and CHOI, D.W. (1987). Quantitative determination of glutamate mediated cortical neuronal injury in cell culture by lactate dehydrogenase efflux assay. J. Neurosci. Meth. 20, 83-90.
14. KOH, J.Y., GWAG, B.G., LOBNER, D., and CHOI, D.W. (1995). Potentiated necrosis of cultured cortical neurons by neutrophins. Science 268, 573-575.
15. KOH, J.Y., SUH, S.W., GWAG, B.J., HE, Y.Y., HSU, C.Y., and CHOI, D.W. (1996). The role of zink in selective neuronal death after transient global cerebral ischemia. Science 272, 1013-1016.
16. LUCAS, J.H., GROSS, G.W., EMERY, D.G., and GARDNER, C.R. (1985). Neuronal survival or death after dendrite transection close to the perikaryon: Correlation with electrophysiologic, morphologic, and ultrastructural changes. CNS Trauma 2, 321-255.
17. LUCAS, J.H., and WOLF, A. (1991). In vitro studies of multiple impact injury to mammalian CNS neurons: prevention of perikaryal damage and death by ketamine. Brain Res. 543, 181-193.
18. MCINTOSH, T.K. (1993). Novel pharmacologic therapies in the treatment of experimental traumatic brain injury: a review. J. Neurotrauma 10, 215-261.
19. MCINTOSH, T.K., SOARES, H.L., HAYES, R.L., and SIMON, R.P. (1989). The N-methyl-D-aspartate antagonists MK-801 prevents edema and restores magnesium homeostasis following traumatic brain injury in rats, in: Recent Advances in Excitatory Amino Acid Research, Leahman J (ed), Alan R. Liss: NY, p. 653.
20. MCINTOSH, T.K., VINK, R., SOARES, H., HAYES, R., and SIMON, R. (1990). Effect of noncompetitive blockade of N-methyl-D-aspartate receptors on the neurochemical sequelae of experimental brain injury. J. Neurochem. 55, 1170-1179.
21. MUKHIN, A.G., FAN, L., and FADEN, A.I. (1996). Activation of metabotropic glutamate receptors subtype mGluR1 contributes to post-traumatic neuronal injury. J. Neurosci. 16, 6012-6120.
22. MURPHY, E.J., and HORROCKS, L.A. (1993). A model for compression trauma: pressure-induced injury in cell cultures. J. Neurotrauma 10, 431-444.
23. MURPHY, T.H., MIYAMOTO, M., SASTRE, A., SCHAAR, R.L., and COYLE, J.T. (1989). Glutamate toxicity in neuronal cell line involves inhibition of cystine transport leading to oxidative stress. Neuron 2, 1547-1558.
24. NILSSON, P., HILLERED, L., PONTEN, U., and UNGERSTEDT, U. (1990). Changes in cortical extracellular levels of energy-related metabolites and amino acids following concussive brain injury in rats. J. Cereb. Blood. Flow Metab. 10, 631.
25. PANTER, S.S., and FADEN, A.I. (1992). Biochemical changes and secondary injury from stroke and trauma, Chapter 4, in: Principles and Practice of Restorative Neurology. Butterworth's: NY, pps. 32-52.
26. PIN, J.-P., and DUVOISIN, R. (1995). Review: Neurotransmitter receptors I. The metabotropic glutamate receptors: structure and functions. Neuropharmacol, 34, 1-26.
27. REGAN, R.F., and CHOI, D.W. (1994). The effect of NMDA, AMPA/Kainate, and calcium channel antagonist on traumatic cortical neuronal injury in culture. Brain Res 633, 236-242.
28. REGAN, R.F., and PANTER, S.S. (1994). Traumatic neuronal injury in cortical cell culture is attenuated by 21-amino-steroids. Soc. Neurosci. Abstr., 20, 192.
29. SHARPIRA, Y., YADID, G., COTEV, A., NISKA, A., and SHOHAMI, E. (1990). Protective effect of MK801 in experimental brain trauma. J. Neurotrauma 7, 131-139.
30. SUN, F.Y., and FADEN, A.I. (1995). Pretreatment with antisense oligodeoxynucleotides directed against the NMDA-R1 receptor enhances survival and behavioral recovery following traumatic brain injury. Brain Res. 693, 163-168.
31. WRATHALL, J.R., TENG, Y.D., CHOINIERE, D., and MUNDT, D.J. (1992). Evidence that local non-NMDA receptors contribute to functional deficites in contusive spinal cord injury. Brain Res. 586, 140-143.
32. 2+ blockade of NMDA current in mechanically injured neurons. Science 274, 1921-1923.