Regulation of brain capillary endothelial cells by P2Y receptors coupled to Ca2+, phospholipase C and mitogen-activated protein kinase

British Journal of Pharmacology

Volumn 122, Issue 5, 1997, Pages 935-941

  Albert, Jennifer L ^a   Boyle, John P ^a   Roberts, Jonathan A ^a   Challiss, R A John ^a   Gubby, Sharon E ^a   Boarder, Michael R ^a  

^a UNIVERSITY OF LEICESTER   (United Kingdom)

Author keywords

Blood brain barrier; Brain endothelial cells; Ca2+ mobilization; Endothelial cells; Inositol (poly)phosphates; Ins (1,4,5)P3; MAPK; Nucleotide receptors; P2 receptors; P2Y receptors

Indexed keywords

2 METHYLTHIOADENOSINE TRIPHOSPHATE; ADENOSINE TRIPHOSPHATE; ALPHA,BETA METHYLENEADENOSINE TRIPHOSPHATE; CALCIUM ION; CELL SURFACE RECEPTOR; CYCLIC AMP; FORSKOLIN; INOSITOL 1,4,5 TRISPHOSPHATE; LITHIUM; MITOGEN ACTIVATED PROTEIN KINASE; NUCLEOTIDE; PHOSPHOLIPASE C; PHOSPHOTRANSFERASE; PURINE P2X RECEPTOR; PURINE P2Y RECEPTOR; URIDINE TRIPHOSPHATE;

ANIMAL CELL; ARTICLE; BLOOD BRAIN BARRIER; BRAIN CAPILLARY; CALCIUM CELL LEVEL; CALCIUM TRANSPORT; CAPILLARY ENDOTHELIUM; CELL CULTURE; CELL STIMULATION; CONCENTRATION RESPONSE; CONTROLLED STUDY; CYTOSOL; ENDOTHELIUM CELL; ENZYME ACTIVITY; ENZYME ASSAY; EXTRACELLULAR CALCIUM; NONHUMAN; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION; RAT; RECEPTOR BINDING; REGULATORY MECHANISM;

ADENOSINE DIPHOSPHATE; ADENOSINE TRIPHOSPHATE; ANIMALS; BLOOD-BRAIN BARRIER; BRAIN; CA(2+)-CALMODULIN DEPENDENT PROTEIN KINASE; CALCIUM; CAPILLARIES; CELLS, CULTURED; CYCLIC AMP; CYTOSOL; ENDOTHELIUM, VASCULAR; ENZYME ACTIVATION; INOSITOL PHOSPHATES; PHOSPHOLIPASE C; PHOSPHORYLATION; RATS; RATS, WISTAR; RECEPTORS, PURINERGIC P2; THIONUCLEOTIDES; URIDINE TRIPHOSPHATE;

EID: 0030778360     PISSN: 00071188     EISSN: None     Source Type: Journal    
DOI: 10.1038/sj.bjp.0701453     Document Type: Article

References (31)

1. 2+ in human erythroleukaemia cells. J. Biol. Chem., 212, 10151-10159.
2. 2 purinoceptors: from molecular biology to functional responses. Trends Pharmacol. Sci., 16, 133-139.
3. 2U-purinoceptor stimulation of prostacyclin release. Br. J. Pharmacol., 116, 2563-2569.
4. 2Y-purinoceptors. Br. J. Pharmacol., 113, 614-620.
5. BROWN, B.L., ALBANO, J.D., EKINS, R.P., SGHERZI, A.M. & TAMPION, W. (1971). A simple and sensitive saturation assay method for the measurement of adenosine 3′, 5′-cyclic monophosphate. Biochem. J., 121, 561-562.
6. BURNSTOCK, G. (1996). P2 purinoceptors: historical perspective and classification. In Ciba Foundation Symposium, Vol. 198, pp. 1-28. Chichester: J. Wiley.
7. 2+ concentration. Br. J. Pharmacol., 95, 1181-1190.
8. CHALLISS, R.A.J., BATTY, I.H. & NAHORSKI, S.R. (1988). Mass measurement of inositol 1,4,5-trisphosphate in rat cerebral cortical slices using a radioreceptor assay: effects of neurotransmitters and depolarisation. Biochem. Biophys. Res. Commun., 157, 684-691.
9. 2Y purinoceptors in rat brain microvascular endothelial cells. Br. J. Pharmacol., 115, 1199-1203.
10. 2+ mobilisation in brain capillary endothelial cells. J. Biol. Chem., 268, 8787-8792.
11. HARDEN, T.K., LAZAROWSKI, E.R. & BOUCHER, R.C. (1997). Release, metabolism and interconversion of adenine and uridine nucleotides: implications for G protein-coupled P2 receptor agonist selectivity. Trends Pharmacol. Sci., 18, 43-45.
12. LUSTIG, K.D., SPORTIELLO, M., ERB, L. & WEISMAN, G.A. (1992). A nucleotide receptor in vascular endothelial cells is specifically activated by the fully ionised forms of ATP and UTP. Biochem. J., 284, 733-739.
13. 2U receptors in inositol phosphate response. Circ. Res., 72, 504-510.
14. NEEDHAM, L., CUSAK, N.J., PEARSON, J.D. & GORDON, J.L. (1987). Characteristics of the P2 purinoceptor that mediates prostacyclin production by pig aortic endothelial cells. Eur. J. Pharmacol., 134, 199-209.
15. NICHOLAS, R.A., WATT, W.C., LAZAROWSKI, E.R., LI, Q. & HARDEN, T.K. (1996). Uridine nucleotide sensitivity of three phospholipase C activating P2 receptors: identification of a UDP-selective, a UTP-selective, and an ATP- and UTP-specific receptor. Mol. Pharmacol., 50, 224-229.
16. NOBLES, M., REVEST, P.A., COURAUD, P.-O. & ABBOTT, N.J. (1995). Characterization of nucleotide receptors that cause elevation of cytoplasmic calcium in immortalised rat brain endothelial cells (RBE4) and in primary cultures. Br. J. Pharmacol., 115, 1245-1252.
17. 2U-purinoceptor-stimulated prostacyclin release. Br. J. Pharmacol., 118, 123-130.
18. PATEL, V., BROWN, C., GOODWIN, A., WILKIE, N. & BOARDER, M.R. (1996b). Phosphorylation and activation of p42 and p44 mitogen activated protein kinase are required for the P2 purinoceptor stimulation of endothelial prostacyclin production. Biochem. J., 320, 221-226.
19. PEARSON, J.D., SLAKEY, L.L. & GORDON, J.L. (1983). Stimulation of prostacyclin production through purinoceptors on cultured porcine endothelial cells. Biochem. J., 214, 273-276.
20. REVEST, P.A. & ABBOTT, N.J. (1992). Membrane ion channels of endothelial cells. Trends Pharmacol. Sci., 13, 404-407.
21. 2+ in cultured rat brain endothelial cells. Brain Res., 549, 159-161.
22. RUBIN, L.L. (1991). A cell culture model of the blood brain barrier. J. Cell Biol., 115, 1725-1735.
23. 2Y1 purinoceptor. Br. J. Pharmacol., 118, 167-173.
24. 2+ stores: activation and function. Trends Neurosci., 18, 299-306.
25. STADDON, J.M., HERRENKNECHT, K., SMALES, C. & RUBIN, L.L. (1995). Evidence that tyrosine phosphorylation may increase tight junction permeability. J. Cell Sci., 108, 609-616.
26. STADDON, J.M. & RUBIN, L.L. (1996). Cell adhesion, cell junctions and the blood brain barrier. Curr. Opin. Neurobiol. 6, 622-627.
27. VIGNE, P., FEOLDE, E., BREITTMAYER, J.P. & FRELIN, C. (1994). Characterisation of the effects of 2-methylthio-ATP and 2-cnloro-ATP on brain capillary endothelial cells. Similarities to ADP and differences from ATP. Br. J. Pharmacol, 112, 775-780.
28. WEBB, T.E., FEOLDE, E., VIGNE, P., NEARY, J.T., RUNBERG, A., FRELIN, C. & BARNARD, E.A. (1996). The P2Y purinoceptor in rat brain microvascular endothelial cells couple to inhibition of adenylate cyclase. Br. J. Pharmacol, 119, 1385-1392.
29. 2 release but has no effect on von Willebrand Factor secretion or E-selectin expression. FEBS Lett 388, 180-185.
30. WILKIE, N., MORTON, C., NG, L. & BOARDER, M.R. (1996). Stimulated mitogen activated protein kinase is necessary but not sufficient for the mitogenic response to angiotensin II. A role for phospholipase D. J. Biol. Chem., 271, 32454-32460.
31. 2Y and nucleotide receptors linked to phospholipase C. Br. J. Pharmacol., 268, 881-887.