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Volumn 277, Issue 5334, 1997, Pages 2005-2007

Selective expression of the eotaxin receptor CCR3 by human T helper 2 cells

Author keywords

[No Author keywords available]

Indexed keywords

INTERLEUKIN 4; INTERLEUKIN 5; T LYMPHOCYTE RECEPTOR;

EID: 0030769920     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.277.5334.2005     Document Type: Article
Times cited : (951)

References (42)
  • 1
    • 0025182959 scopus 로고
    • T. A. Springer, Nature 346, 425 (1990); E. C. Butcher and L. J. Picker, Science 272, 60 (1996).
    • (1990) Nature , vol.346 , pp. 425
    • Springer, T.A.1
  • 2
    • 0030001675 scopus 로고    scopus 로고
    • T. A. Springer, Nature 346, 425 (1990); E. C. Butcher and L. J. Picker, Science 272, 60 (1996).
    • (1996) Science , vol.272 , pp. 60
    • Butcher, E.C.1    Picker, L.J.2
  • 4
    • 0026342111 scopus 로고
    • E. C. Butcher, Cell 67, 1033 (1991); T. A. Springer, ibid. 76, 301 (1994).
    • (1991) Cell , vol.67 , pp. 1033
    • Butcher, E.C.1
  • 5
    • 0027982876 scopus 로고
    • E. C. Butcher, Cell 67, 1033 (1991); T. A. Springer, ibid. 76, 301 (1994).
    • (1994) Cell , vol.76 , pp. 301
    • Springer, T.A.1
  • 7
    • 0028288179 scopus 로고
    • T. R. Mosmann and R. L. Coffman, Annu. Rev. Immunol. 7, 145 (1989); S. Romagnani, ibid. 12, 227 (1994).
    • (1994) Annu. Rev. Immunol. , vol.12 , pp. 227
    • Romagnani, S.1
  • 8
    • 0031012138 scopus 로고    scopus 로고
    • F. Austrup et al., Nature 385, 81 (1997).
    • (1997) Nature , vol.385 , pp. 81
    • Austrup, F.1
  • 10
    • 13344283419 scopus 로고    scopus 로고
    • P. D. Ponath et al., J. Clin. Invest. 97, 604 (1996); P. J. Jose et al., J. Exp. Med. 179, 881 (1994); M. Kitaura et al., J. Biol. Chem. 271, 7725 (1996); E. A. Garcia-Zepeda et al., Nature Med. 2, 449 (1996).
    • (1996) J. Clin. Invest. , vol.97 , pp. 604
    • Ponath, P.D.1
  • 11
    • 0028140133 scopus 로고
    • P. D. Ponath et al., J. Clin. Invest. 97, 604 (1996); P. J. Jose et al., J. Exp. Med. 179, 881 (1994); M. Kitaura et al., J. Biol. Chem. 271, 7725 (1996); E. A. Garcia-Zepeda et al., Nature Med. 2, 449 (1996).
    • (1994) J. Exp. Med. , vol.179 , pp. 881
    • Jose, P.J.1
  • 12
    • 0029980492 scopus 로고    scopus 로고
    • P. D. Ponath et al., J. Clin. Invest. 97, 604 (1996); P. J. Jose et al., J. Exp. Med. 179, 881 (1994); M. Kitaura et al., J. Biol. Chem. 271, 7725 (1996); E. A. Garcia-Zepeda et al., Nature Med. 2, 449 (1996).
    • (1996) J. Biol. Chem. , vol.271 , pp. 7725
    • Kitaura, M.1
  • 13
    • 0029944290 scopus 로고    scopus 로고
    • P. D. Ponath et al., J. Clin. Invest. 97, 604 (1996); P. J. Jose et al., J. Exp. Med. 179, 881 (1994); M. Kitaura et al., J. Biol. Chem. 271, 7725 (1996); E. A. Garcia-Zepeda et al., Nature Med. 2, 449 (1996).
    • (1996) Nature Med. , vol.2 , pp. 449
    • Garcia-Zepeda, E.A.1
  • 14
    • 0029951387 scopus 로고    scopus 로고
    • B. L. Daugherty et al., J. Exp. Med. 183, 2349 (1996); P. D. Ponath et al., ibid., p. 2437; M. G. Uguccioni et al., J. Clin. Invest., in press.
    • (1996) J. Exp. Med. , vol.183 , pp. 2349
    • Daugherty, B.L.1
  • 15
    • 0029951387 scopus 로고    scopus 로고
    • B. L. Daugherty et al., J. Exp. Med. 183, 2349 (1996); P. D. Ponath et al., ibid., p. 2437; M. G. Uguccioni et al., J. Clin. Invest., in press.
    • J. Exp. Med. , pp. 2437
    • Ponath, P.D.1
  • 16
    • 0029951387 scopus 로고    scopus 로고
    • in press
    • B. L. Daugherty et al., J. Exp. Med. 183, 2349 (1996); P. D. Ponath et al., ibid., p. 2437; M. G. Uguccioni et al., J. Clin. Invest., in press.
    • J. Clin. Invest.
    • Uguccioni, M.G.1
  • 18
    • 85069406866 scopus 로고    scopus 로고
    • note
    • + cells (25).
  • 19
    • 85069408829 scopus 로고    scopus 로고
    • note
    • -7 M phorbol 12-myristate 13-acetate (PMA). Cytokine production was measured in the 24-hour culture supernatant by enzyme-linked immunosorbent assay (ELISA), using matched pairs of antibodies specific for IL-2, IL-4, IL-5, IL-10, tumor necrosis factor-α (TNF-α), TNF-β, and IFN-γ (PharMingen, San Diego, CA).
  • 20
    • 85069412966 scopus 로고    scopus 로고
    • note
    • -7 M PMA plus ionomycin (1 μg/ml) for 4 hours. Brefeldin A (10 μg/ml) was added during the last 2 hours. Cells were fixed with 2% paraformaldehyde, permeabilized with phosphate-buffered saline containing fetal bovine serum (1%) and saponin (0.5%) and stained with fluorescein isothiocyanate (FITC)-labeled anti-IFN-γ (IgG1) and phycoerythrin (PE)-labeled anti-IL-4 (IgG2b) monoclonal antibodies (Becton Dickinson, Mountain View, CA). In some experiments, the cells were stained, before permeabilization, with anti-CD4 (BL4, IgG2a, Immunotech, Marseille, France) or anti-CD8 (OKT8, IgG2a, American Type Culture Collection), then with biotin-labeled goat anti-mouse IgG2a (Southern Biotechnology, Birmingham, AL) and streptavidin-tricolor (Molecular Probes, Eugene, OR). Because T cell activation results in a rapid down-regulation of CCR3, it is not possible to assess CCR3 expression and intracellular cytokines simultaneously.
  • 21
    • 85069413632 scopus 로고    scopus 로고
    • note
    • + cells. Results comparable to those in Fig. 1 were obtained in sorted cell lines obtained from two healthy and three atopic individuals. In three out of five cases the CCR3-depleted cells produced lower concentrations of IL-4 and IL-5 than the unsorted cells. No clear differences were found in the production of IL-10, IFN-γ, IL-2, TNF-α, or TNF-β, or between healthy and atopic donors. A correlation between CCR3 expression and production of IL-4 but not IFN-γ was observed in 13 antigen-specific T cell clones (14).
  • 22
    • 85069407201 scopus 로고    scopus 로고
    • unpublished data
    • F. Sallusto, unpublished data.
    • Sallusto, F.1
  • 31
    • 85069404151 scopus 로고    scopus 로고
    • unpublished data
    • C. R. MacKay, unpublished data. Chemotaxis experiments were done as described by P. D. Ponath et al. (7), with a transendothelial assay and eotaxin or MCP-1 at 10 nM and IP-10 at 20 nM. Cells migrating to the bottom of the transwell chemotaxis chamber were counted by flow cytometry.
    • MacKay, C.R.1
  • 33
    • 0030980816 scopus 로고    scopus 로고
    • Eotaxin is not the only agonist for CCR3, because MCP-3, MCP-4, RANTES (2), and eotaxin 2 [U. Forrsmann et al., J. Exp. Med. 185, 2171 (1997)] can also bind and trigger CCR3.
    • (1997) J. Exp. Med. , vol.185 , pp. 2171
    • Forrsmann, U.1
  • 34
    • 0026443197 scopus 로고
    • C. J. Corrigan and A. B. Kay, Immunol. Today 13, 501 (1992); J. M. Drazen, J. P. Arm, K. F. Austen, J. Exp. Med. 183, 1 (1996).
    • (1992) Immunol. Today , vol.13 , pp. 501
    • Corrigan, C.J.1    Kay, A.B.2
  • 36
  • 37
    • 0026610142 scopus 로고
    • D. S. Robinson et al., N. Engl. J. Med. 326, 298 (1992); G. F. Del Prete et al., Eur. J. Immunol. 23, 1445 (1993); S. H. Gavett, X. Chen, F. Finkelman, M. Wills Karp, Am. J. Respir. Cell. Mol. Biol. 10, 587 (1994).
    • (1992) N. Engl. J. Med. , vol.326 , pp. 298
    • Robinson, D.S.1
  • 38
    • 0027197709 scopus 로고
    • D. S. Robinson et al., N. Engl. J. Med. 326, 298 (1992); G. F. Del Prete et al., Eur. J. Immunol. 23, 1445 (1993); S. H. Gavett, X. Chen, F. Finkelman, M. Wills Karp, Am. J. Respir. Cell. Mol. Biol. 10, 587 (1994).
    • (1993) Eur. J. Immunol. , vol.23 , pp. 1445
    • Del Prete, G.F.1
  • 42
    • 85069413059 scopus 로고    scopus 로고
    • note
    • We thank A. Mari and F. Nestle for providing samples from allergic patients; M. Dessing and A. Pickert for cell sorting; D. Lenig and H. Heath for expert technical assistance; and M. Cella, M. Colonna, and M. Kopf for critical reading and comments. The Basel Institute for Immunology was founded and is supported by F. Hoffmann-La Roche Ltd., Basel, Switzerland. F. S. is also with the department of immunology, Istituto Superiore de Sanità, Rome.


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