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Requirement of the Paraxis gene for somite formation and musculoskeletal patterning
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of outstanding interest. This bHLH gene, which is expressed in paraxial mesoderm, is essential for somite epithelialization. Nevertheless, metamerism and segmentation take place in null mice and somitic compartments form although they appear disorganised.
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of special interest. By targeting Myf5 with the nlacZ reporter gene, these authors show that muscle progenitors are present in the embryo in the absence of any MRF. These progenitors remain multipotential and can change fate. This study therefore provides direct evidence that Myf5 acts in the final stages of determination to confer myogenic identity.
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Tajbakhsh, S.1
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of outstanding interest. Until now, it has been suggested that Myf5 and MyoD act in parallel genetic pathways. This study now places MyoD genetically downstream of Pax3 and Myf5. The latter two genes are activated independently of each other in the embryo. Furthermore, MyoD activation was shown to be dependent on Myf5; MyoD activation via Pax3 takes place subsequently. Pax3 plays a role in long-range muscle progenitor cell migrations although, unexpectedly, it is also important for epaxial muscle development. Remarkably, in the absence of Pax3 and Myf5, head muscles form, probably via MyoD.
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