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Volumn 278, Issue 5338, 1997, Pages 695-698

Inhibition of HIV-1 infection by the β-chemokine MDC

Author keywords

[No Author keywords available]

Indexed keywords

CD8 ANTIGEN; CHEMOKINE;

EID: 0030722896     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.278.5338.695     Document Type: Article
Times cited : (191)

References (56)
  • 2
    • 0025351001 scopus 로고
    • C. M. Walker, D. J. Moody, D. P. Stites, J. A. Levy, Science 234, 1563 (1986); J. E. Brinchman et al., J. Immunol. 144, 2961 (1990).
    • (1990) J. Immunol. , vol.144 , pp. 2961
    • Brinchman, J.E.1
  • 3
    • 0024519892 scopus 로고
    • C. M. Walker, D. Moody, D. P. Stites, J. A. Levy, Cell. Immunol. 119, 470 (1989); C. E. Mackewicz, H. W. Ortega, J. S. Levy, J. Clin. Invest. 87, 1462 (1991); D. J. Blackbourn et al., Proc. Natl. Acad. Sci. U.S.A. 93, 13125 (1996).
    • (1989) Cell. Immunol. , vol.119 , pp. 470
    • Walker, C.M.1    Moody, D.2    Stites, D.P.3    Levy, J.A.4
  • 4
    • 0025891987 scopus 로고
    • C. M. Walker, D. Moody, D. P. Stites, J. A. Levy, Cell. Immunol. 119, 470 (1989); C. E. Mackewicz, H. W. Ortega, J. S. Levy, J. Clin. Invest. 87, 1462 (1991); D. J. Blackbourn et al., Proc. Natl. Acad. Sci. U.S.A. 93, 13125 (1996).
    • (1991) J. Clin. Invest. , vol.87 , pp. 1462
    • Mackewicz, C.E.1    Ortega, H.W.2    Levy, J.S.3
  • 5
    • 0029803743 scopus 로고    scopus 로고
    • C. M. Walker, D. Moody, D. P. Stites, J. A. Levy, Cell. Immunol. 119, 470 (1989); C. E. Mackewicz, H. W. Ortega, J. S. Levy, J. Clin. Invest. 87, 1462 (1991); D. J. Blackbourn et al., Proc. Natl. Acad. Sci. U.S.A. 93, 13125 (1996).
    • (1996) Proc. Natl. Acad. Sci. U.S.A. , vol.93 , pp. 13125
    • Blackbourn, D.J.1
  • 6
    • 0029417004 scopus 로고    scopus 로고
    • F. Cocchi et al., Science 270, 1811 (1996).
    • (1996) Science , vol.270 , pp. 1811
    • Cocchi, F.1
  • 7
    • 0029889608 scopus 로고    scopus 로고
    • T. D. Barker, D. Weissman, J. A. Daucher, K. Roche, A. Fauci, J. Immunol. 156, 4476 (1996); X. Paliard, A. Y. Lee, C. M. Walker, AIDS 10, 1317 (1996); A. L. Kinter et al., Proc. Natl. Acad. Sci. U.S.A. 93, 14076 (1996); A. Rubbert et al., AIDS Res. Hum. Retroviruses 13, 63 (1997).
    • (1996) J. Immunol. , vol.156 , pp. 4476
    • Barker, T.D.1    Weissman, D.2    Daucher, J.A.3    Roche, K.4    Fauci, A.5
  • 8
    • 0029768925 scopus 로고    scopus 로고
    • T. D. Barker, D. Weissman, J. A. Daucher, K. Roche, A. Fauci, J. Immunol. 156, 4476 (1996); X. Paliard, A. Y. Lee, C. M. Walker, AIDS 10, 1317 (1996); A. L. Kinter et al., Proc. Natl. Acad. Sci. U.S.A. 93, 14076 (1996); A. Rubbert et al., AIDS Res. Hum. Retroviruses 13, 63 (1997).
    • (1996) AIDS , vol.10 , pp. 1317
    • Paliard, X.1    Lee, A.Y.2    Walker, C.M.3
  • 9
    • 0030465244 scopus 로고    scopus 로고
    • T. D. Barker, D. Weissman, J. A. Daucher, K. Roche, A. Fauci, J. Immunol. 156, 4476 (1996); X. Paliard, A. Y. Lee, C. M. Walker, AIDS 10, 1317 (1996); A. L. Kinter et al., Proc. Natl. Acad. Sci. U.S.A. 93, 14076 (1996); A. Rubbert et al., AIDS Res. Hum. Retroviruses 13, 63 (1997).
    • (1996) Proc. Natl. Acad. Sci. U.S.A. , vol.93 , pp. 14076
    • Kinter, A.L.1
  • 10
    • 0031020988 scopus 로고    scopus 로고
    • T. D. Barker, D. Weissman, J. A. Daucher, K. Roche, A. Fauci, J. Immunol. 156, 4476 (1996); X. Paliard, A. Y. Lee, C. M. Walker, AIDS 10, 1317 (1996); A. L. Kinter et al., Proc. Natl. Acad. Sci. U.S.A. 93, 14076 (1996); A. Rubbert et al., AIDS Res. Hum. Retroviruses 13, 63 (1997).
    • (1997) AIDS Res. Hum. Retroviruses , vol.13 , pp. 63
    • Rubbert, A.1
  • 12
    • 0020628373 scopus 로고
    • + phenotype of the immortalized cell lines was verified by flow cytometry with antibodies to CD8 and to CD4 (Becton-Dickinson, San Jose, CA).
    • (1983) Int. J. Cancer , vol.31 , pp. 413
    • Markham, P.D.1
  • 13
    • 1842407074 scopus 로고
    • + phenotype of the immortalized cell lines was verified by flow cytometry with antibodies to CD8 and to CD4 (Becton-Dickinson, San Jose, CA).
    • (1984) Int. J. Cancer , vol.33 , pp. 13
  • 16
    • 1842371947 scopus 로고    scopus 로고
    • T. Dragic et al., Nature 381, 661 (1996); G. Alkhatib et al., Science 272, 1955 (1996); H. Deng et al., Nature 381, 661 (1996).
    • (1996) Nature , vol.381 , pp. 661
    • Dragic, T.1
  • 17
    • 0030018156 scopus 로고    scopus 로고
    • T. Dragic et al., Nature 381, 661 (1996); G. Alkhatib et al., Science 272, 1955 (1996); H. Deng et al., Nature 381, 661 (1996).
    • (1996) Science , vol.272 , pp. 1955
    • Alkhatib, G.1
  • 18
    • 15844419153 scopus 로고    scopus 로고
    • T. Dragic et al., Nature 381, 661 (1996); G. Alkhatib et al., Science 272, 1955 (1996); H. Deng et al., Nature 381, 661 (1996).
    • (1996) Nature , vol.381 , pp. 661
    • Deng, H.1
  • 20
    • 1842405260 scopus 로고    scopus 로고
    • note
    • 2O containing 0.1% TFA, and fractionated under the same conditions to obtain a single protein peak. The fraction corresponding to the peak and flanking fractions were tested in the infectivity assay to verify that suppressor activity cofractionated with the protein.
  • 21
  • 22
    • 0030220158 scopus 로고    scopus 로고
    • D. P. Witt and A. D. Lander, Curr. Biol. 4, 394 (1994); P. Proost et al., Methods 10, 82 (1996).
    • (1996) Methods , vol.10 , pp. 82
    • Proost, P.1
  • 24
    • 1842372568 scopus 로고    scopus 로고
    • note
    • 2-terminal microsequencing was performed by automated Edman degradation with a Hewlett-Packard G1005A protein sequencing system, which was operated with standard reagents, solvents, and programs (routine 3.0) supplied by the manufacturer. Abbreviations for the amino acid residues are A, Ala; D, Asp; E, Glu; G, Gly; L, Leu; M, Met; N, Asn; P, Pro; R, Arg; S, Ser; V, Val; and Y, Tyr.
  • 26
    • 1842327300 scopus 로고
    • I. Lindley et al., Proc. Natl. Acad. Sci. U.S.A. 85, 9199 (1988); T. Yoshimura et al., Mol. Immunol. 26, 87 (1989); J. VanDamme et al., Eur. J. Biochem. 181, 337 (1989); C. A. Hebert et al., J. Immunol. 145, 3033 (1990).
    • (1988) Proc. Natl. Acad. Sci. U.S.A. , vol.85 , pp. 9199
    • Lindley, I.1
  • 27
    • 0024528879 scopus 로고
    • I. Lindley et al., Proc. Natl. Acad. Sci. U.S.A. 85, 9199 (1988); T. Yoshimura et al., Mol. Immunol. 26, 87 (1989); J. VanDamme et al., Eur. J. Biochem. 181, 337 (1989); C. A. Hebert et al., J. Immunol. 145, 3033 (1990).
    • (1989) Mol. Immunol. , vol.26 , pp. 87
    • Yoshimura, T.1
  • 28
    • 0024604253 scopus 로고
    • I. Lindley et al., Proc. Natl. Acad. Sci. U.S.A. 85, 9199 (1988); T. Yoshimura et al., Mol. Immunol. 26, 87 (1989); J. VanDamme et al., Eur. J. Biochem. 181, 337 (1989); C. A. Hebert et al., J. Immunol. 145, 3033 (1990).
    • (1989) Eur. J. Biochem. , vol.181 , pp. 337
    • VanDamme, J.1
  • 29
    • 0025016210 scopus 로고
    • I. Lindley et al., Proc. Natl. Acad. Sci. U.S.A. 85, 9199 (1988); T. Yoshimura et al., Mol. Immunol. 26, 87 (1989); J. VanDamme et al., Eur. J. Biochem. 181, 337 (1989); C. A. Hebert et al., J. Immunol. 145, 3033 (1990).
    • (1990) J. Immunol. , vol.145 , pp. 3033
    • Hebert, C.A.1
  • 30
    • 0025021678 scopus 로고
    • A. Walz and M. Baggiolini, J. Exp. Med. 171, 449 (1990); J. VanDamme et al., Eur. J. Immunol. 20, 2113 (1990); I. Clark-Lewis, C. Schumacher, M. Baggiolini, B. Moser, J. Biol. Chem. 266, 23128 (1991).
    • (1990) J. Exp. Med. , vol.171 , pp. 449
    • Walz, A.1    Baggiolini, M.2
  • 31
    • 0025185152 scopus 로고
    • A. Walz and M. Baggiolini, J. Exp. Med. 171, 449 (1990); J. VanDamme et al., Eur. J. Immunol. 20, 2113 (1990); I. Clark-Lewis, C. Schumacher, M. Baggiolini, B. Moser, J. Biol. Chem. 266, 23128 (1991).
    • (1990) Eur. J. Immunol. , vol.20 , pp. 2113
    • VanDamme, J.1
  • 33
    • 0027511738 scopus 로고
    • S. C. Bischoff et al., Eur. J. Immunol. 23, 761 (1993); M. Baggiolini, B. Dewald, B. Moser, Adv. Immunol. 55, 97 (1994); K. B. Bacon, B. A. Premack, P. Gardner, T. J. Schall, Science 269, 1727 (1995).
    • (1993) Eur. J. Immunol. , vol.23 , pp. 761
    • Bischoff, S.C.1
  • 34
    • 0028052968 scopus 로고
    • S. C. Bischoff et al., Eur. J. Immunol. 23, 761 (1993); M. Baggiolini, B. Dewald, B. Moser, Adv. Immunol. 55, 97 (1994); K. B. Bacon, B. A. Premack, P. Gardner, T. J. Schall, Science 269, 1727 (1995).
    • (1994) Adv. Immunol. , vol.55 , pp. 97
    • Baggiolini, M.1    Dewald, B.2    Moser, B.3
  • 35
    • 0029096916 scopus 로고
    • S. C. Bischoff et al., Eur. J. Immunol. 23, 761 (1993); M. Baggiolini, B. Dewald, B. Moser, Adv. Immunol. 55, 97 (1994); K. B. Bacon, B. A. Premack, P. Gardner, T. J. Schall, Science 269, 1727 (1995).
    • (1995) Science , vol.269 , pp. 1727
    • Bacon, K.B.1    Premack, B.A.2    Gardner, P.3    Schall, T.J.4
  • 36
    • 1842291439 scopus 로고    scopus 로고
    • in press
    • 6) were then added to sample tubes, loaded for 20 min at 37°C with 2 μM fluo-3 (Molecular Probes) reconstituted in a solution containing 20% Pluronic F-127 (Molecular Probes) and dimethyl sulfoxide, and stained with 7-aminoactinomycin D (Molecular Probes) to discriminate dead cells [I. Schmid et al., Cytometry 13, 204 (1992)]. The samples were then washed once as before and resuspended in 1 ml of RPMI 1640 without phenol red and sodium bicarbonate. All samples were maintained at 20°C in the dark until 5 min before analysis, at which time the sample tube was placed in a 37°C water bath. Cells were maintained at 37°C throughout data acquisition. Cells were stimulated by addition of test chemokine to a final concentration of 3 nM. Data were acquired with a FACSCalibur (Becton-Dickinson) flow cytometer, with excitation at 488 nm. Cells were gated by forward-and side-scatter properties as well as by exclusion of 7-aminoactinomycin D fluorescence by use of emission above 650 nm in the FL-3 window. Calcium mobilization was determined by a two-parameter density plot of linear emissions collected at 530 nm in the FL-1 window for the gated cell population over time.
    • Biotechniques
    • Burns, J.1    Lewis, G.2
  • 37
    • 0029126986 scopus 로고
    • 6) were then added to sample tubes, loaded for 20 min at 37°C with 2 μM fluo-3 (Molecular Probes) reconstituted in a solution containing 20% Pluronic F-127 (Molecular Probes) and dimethyl sulfoxide, and stained with 7-aminoactinomycin D (Molecular Probes) to discriminate dead cells [I. Schmid et al., Cytometry 13, 204 (1992)]. The samples were then washed once as before and resuspended in 1 ml of RPMI 1640 without phenol red and sodium bicarbonate. All samples were maintained at 20°C in the dark until 5 min before analysis, at which time the sample tube was placed in a 37°C water bath. Cells were maintained at 37°C throughout data acquisition. Cells were stimulated by addition of test chemokine to a final concentration of 3 nM. Data were acquired with a FACSCalibur (Becton-Dickinson) flow cytometer, with excitation at 488 nm. Cells were gated by forward-and side-scatter properties as well as by exclusion of 7-aminoactinomycin D fluorescence by use of emission above 650 nm in the FL-3 window. Calcium mobilization was determined by a two-parameter density plot of linear emissions collected at 530 nm in the FL-1 window for the gated cell population over time.
    • (1995) J. Immunol. , vol.155 , pp. 4004
    • Badolato, R.1
  • 38
    • 0029966727 scopus 로고    scopus 로고
    • 6) were then added to sample tubes, loaded for 20 min at 37°C with 2 μM fluo-3 (Molecular Probes) reconstituted in a solution containing 20% Pluronic F-127 (Molecular Probes) and dimethyl sulfoxide, and stained with 7-aminoactinomycin D (Molecular Probes) to discriminate dead cells [I. Schmid et al., Cytometry 13, 204 (1992)]. The samples were then washed once as before and resuspended in 1 ml of RPMI 1640 without phenol red and sodium bicarbonate. All samples were maintained at 20°C in the dark until 5 min before analysis, at which time the sample tube was placed in a 37°C water bath. Cells were maintained at 37°C throughout data acquisition. Cells were stimulated by addition of test chemokine to a final concentration of 3 nM. Data were acquired with a FACSCalibur (Becton-Dickinson) flow cytometer, with excitation at 488 nm. Cells were gated by forward-and side-scatter properties as well as by exclusion of 7-aminoactinomycin D fluorescence by use of emission above 650 nm in the FL-3 window. Calcium mobilization was determined by a two-parameter density plot of linear emissions collected at 530 nm in the FL-1 window for the gated cell population over time.
    • (1996) Cytometry , vol.23 , pp. 205
    • Greimers, R.1
  • 39
    • 0026556554 scopus 로고
    • 6) were then added to sample tubes, loaded for 20 min at 37°C with 2 μM fluo-3 (Molecular Probes) reconstituted in a solution containing 20% Pluronic F-127 (Molecular Probes) and dimethyl sulfoxide, and stained with 7-aminoactinomycin D (Molecular Probes) to discriminate dead cells [I. Schmid et al., Cytometry 13, 204 (1992)]. The samples were then washed once as before and resuspended in 1 ml of RPMI 1640 without phenol red and sodium bicarbonate. All samples were maintained at 20°C in the dark until 5 min before analysis, at which time the sample tube was placed in a 37°C water bath. Cells were maintained at 37°C throughout data acquisition. Cells were stimulated by addition of test chemokine to a final concentration of 3 nM. Data were acquired with a FACSCalibur (Becton-Dickinson) flow cytometer, with excitation at 488 nm. Cells were gated by forward-and side-scatter properties as well as by exclusion of 7-aminoactinomycin D fluorescence by use of emission above 650 nm in the FL-3 window. Calcium mobilization was determined by a two-parameter density plot of linear emissions collected at 530 nm in the FL-1 window for the gated cell population over time.
    • (1992) Cytometry , vol.13 , pp. 204
    • Schmid, I.1
  • 40
    • 0029154824 scopus 로고
    • PBMCs (American Red Cross) from a healthy donor were purified by centrifugation in Histopaque (Sigma) and harvested either immediately or after activation for 48 hours with PHA (5 μg/ml) and rIL-2 (10 ng/ml). The HUT 78 human T cell line was cultured in the presence (50 U/ml) or absence of IL-2 (Boehringer, Mannheim, Germany). RNA was isolated by the RNAzol procedure (Tel-Test, Friendswood, TX), and 10 μg of total cellular RNA was separated by electrophoresis on a denaturing formaldehyde-agarose gel and then transferred to a nylon membrane. The membrane was subjected to hybridization with an MDC-specific probe and washed under stringent conditions as described [A. Garzino-Demo, R. C. Gallo, S. K. Arya, Hum. Gene Ther. 6, 177 (1995)]. The probe for Northern hybridizations was generated by reverse transcription and polymerase chain reaction with MDC-specific primers.
    • (1995) Hum. Gene Ther. , vol.6 , pp. 177
    • Garzino-Demo, A.1    Gallo, R.C.2    Arya, S.K.3
  • 42
    • 0030002637 scopus 로고    scopus 로고
    • Y. Feng, C. C. Broder, P. E. Kennedy, E. A. Berger, Science 272, 872 (1996); H. Choe et al., Cell 85, 1135 (1996); B. J. Doranz et al., ibid., p. 1149; J. F. Berson et al., J. Virol. 70, 6288 (1996).
    • (1996) Science , vol.272 , pp. 872
    • Feng, Y.1    Broder, C.C.2    Kennedy, P.E.3    Berger, E.A.4
  • 43
    • 0005014748 scopus 로고    scopus 로고
    • Y. Feng, C. C. Broder, P. E. Kennedy, E. A. Berger, Science 272, 872 (1996); H. Choe et al., Cell 85, 1135 (1996); B. J. Doranz et al., ibid., p. 1149; J. F. Berson et al., J. Virol. 70, 6288 (1996).
    • (1996) Cell , vol.85 , pp. 1135
    • Choe, H.1
  • 44
    • 0030002637 scopus 로고    scopus 로고
    • Y. Feng, C. C. Broder, P. E. Kennedy, E. A. Berger, Science 272, 872 (1996); H. Choe et al., Cell 85, 1135 (1996); B. J. Doranz et al., ibid., p. 1149; J. F. Berson et al., J. Virol. 70, 6288 (1996).
    • Cell , pp. 1149
    • Doranz, B.J.1
  • 45
    • 0029821869 scopus 로고    scopus 로고
    • Y. Feng, C. C. Broder, P. E. Kennedy, E. A. Berger, Science 272, 872 (1996); H. Choe et al., Cell 85, 1135 (1996); B. J. Doranz et al., ibid., p. 1149; J. F. Berson et al., J. Virol. 70, 6288 (1996).
    • (1996) J. Virol. , vol.70 , pp. 6288
    • Berson, J.F.1
  • 46
    • 1842410045 scopus 로고    scopus 로고
    • C. Bluel et al., Nature 382, 829 (1996); E. Oberlin et al., ibid., p. 833.
    • (1996) Nature , vol.382 , pp. 829
    • Bluel, C.1
  • 47
    • 1842298617 scopus 로고    scopus 로고
    • C. Bluel et al., Nature 382, 829 (1996); E. Oberlin et al., ibid., p. 833.
    • Nature , pp. 833
    • Oberlin, E.1
  • 48
    • 19244380883 scopus 로고    scopus 로고
    • J. He et al., ibid. 385, 645 (1997).
    • (1997) Nature , vol.385 , pp. 645
    • He, J.1
  • 49
    • 16044364731 scopus 로고    scopus 로고
    • L. Zhang, Y. Huang, T. He, Y. Cao, D. D. Ho, ibid. 383, 768 (1996); M. T. Dittmar et al., ibid. 385, 495 (1997); G. Simmons, J. Virol. 70, 8355 (1996).
    • (1996) Nature , vol.383 , pp. 768
    • Zhang, L.1    Huang, Y.2    He, T.3    Cao, Y.4    Ho, D.D.5
  • 50
    • 0031028817 scopus 로고    scopus 로고
    • L. Zhang, Y. Huang, T. He, Y. Cao, D. D. Ho, ibid. 383, 768 (1996); M. T. Dittmar et al., ibid. 385, 495 (1997); G. Simmons, J. Virol. 70, 8355 (1996).
    • (1997) Nature , vol.385 , pp. 495
    • Dittmar, M.T.1
  • 51
    • 10344266976 scopus 로고    scopus 로고
    • L. Zhang, Y. Huang, T. He, Y. Cao, D. D. Ho, ibid. 383, 768 (1996); M. T. Dittmar et al., ibid. 385, 495 (1997); G. Simmons, J. Virol. 70, 8355 (1996).
    • (1996) J. Virol. , vol.70 , pp. 8355
    • Simmons, G.1
  • 52
    • 0023095065 scopus 로고
    • S. Patterson and S. C. Knight, J. Gen. Virol. 68, 1177 (1987); J. Embretson et al., Nature 362, 359 (1993); M. Pope et al., J. Exp. Med. 182, 2045 (1995).
    • (1987) J. Gen. Virol. , vol.68 , pp. 1177
    • Patterson, S.1    Knight, S.C.2
  • 53
    • 0027516822 scopus 로고
    • S. Patterson and S. C. Knight, J. Gen. Virol. 68, 1177 (1987); J. Embretson et al., Nature 362, 359 (1993); M. Pope et al., J. Exp. Med. 182, 2045 (1995).
    • (1993) Nature , vol.362 , pp. 359
    • Embretson, J.1
  • 54
    • 0028809511 scopus 로고
    • S. Patterson and S. C. Knight, J. Gen. Virol. 68, 1177 (1987); J. Embretson et al., Nature 362, 359 (1993); M. Pope et al., J. Exp. Med. 182, 2045 (1995).
    • (1995) J. Exp. Med. , vol.182 , pp. 2045
    • Pope, M.1
  • 56
    • 1842327299 scopus 로고    scopus 로고
    • note
    • We thank B. Lambe for technical assistance; A. Wiznia, G. Lambert, M. Rosenberg, J. Dobroszycki, and O. Cohen for clinical samples; N. Miller for helpful discussions and support for our search for new HIV-1-suppressor chemokines; K. Swiderek for amino acid analysis and microsequencing; and R. Connor for primary virus isolates. R.P., M.B., and P.D.M. were supported in part by National Institute of Allergy and Infectious Diseases contract NO1-AI-55279 to Advanced BioScience Laboratories.


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