The Semliki Forest virus system: Receptor expression and scale-up

Genetic Engineer and Biotechnologist

Volumn 17, Issue 2-3, 1997, Pages 101-106

  Lundstrom', Kenneth ^a,c   Blasey, Horst ^a,b   Hovius, Ruud ^a,b   Vogel, Horst ^a,b   Bernard, Alain ^a,b  

^a F HOFFMANN LA ROCHE LTD   (Switzerland)

^b GlaxoSmithKline   (Switzerland)

^c EPFL   (Switzerland)

Author keywords

Mammalian expression; Primary neurons; Scale up; Semliki Forest virus

Indexed keywords

GUANINE NUCLEOTIDE BINDING PROTEIN; ION CHANNEL; RECOMBINANT PROTEIN; SEROTONIN 3 RECEPTOR; VIRUS RECEPTOR;

BINDING AFFINITY; CHANNEL GATING; CONFERENCE PAPER; ELECTROPHYSIOLOGY; NONHUMAN; PATCH CLAMP; PRIORITY JOURNAL; SEMLIKI FOREST ALPHAVIRUS; SITE DIRECTED MUTAGENESIS;

ALPHAVIRUS; MAMMALIA; RABBIT FIBROMA VIRUS; SEMLIKI FOREST VIRUS;

EID: 0030693198     PISSN: 0959020X     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Conference Paper

References (14)

1. Berglund, P., Sjoberg, M., Garoff, H. et al. (1993) Semliki Forest virus expression system: production of conditionally infectious recombinant particles. Bio/ Technology, 11, 916-920.
2. Blasey, H.D., Brethon, B., Lundstrom, K. et al. (1997a) 5-HTs receptor expression with the Semliki Forest virus at reactor scale (in press).
3. Blasey, H.D., Lundstrom, K., Täte, S. et al. (1997b) Recombinant protein production using the Semliki Forest virus expression system. Cytotechnology, 24, 65-72.
4. Huckriede, A., Heikema, A., Wilschut, J. et al. (1996) Transient expression of a mitochondrial precursor protein: a new approach to study mitochondrial protein import in cells of higher eukaryotes. European Journal of Biochemistry, 237, 288-294.
5. Hovius, R., Tain, A.-P., Blasey, H.D. et al. (1997) Characterization of the recombinant mouse 5-HTs serotonin receptor purified from mammalian cells. Journal of Neurochemisty (in press).
6. Liljestrom, P. & Garoff, H. (1991) A new generation of animal cell expression vectors based on the Semliki Forest virus replicon. Bio /Technolog)/, 9, 1356-1361.
7. Lundstrom, K. (1996) Application of the SFV expression system for binding and functional studies of receptors and ion channels. Focus, 18, 53-56.
8. Lundstrom, K. (1997) Alphaviruses as expression vectors period. Current opinion in biotechnology, 8, 578-2582.
9. Lundstrom, K. & Turpin, M.P. (1996) Proposed schizophrenia-related gene polymorphism: expression of the ser9gly mutant human dopamine DS receptor with the Semliki Forest virus system. Biochemical Biophysical Research Communications, 225, 1068-1072.
10. Lundstrom, K., Mills, A., Buell, G. et al. (1994) High-level expression of the human neurokinin-1 receptor in mammalian cell lines using the Semliki Forest virus expression system. European Journal of Biochemistry, 224, 917-921.
11. Olkkonen, V.M., Liljestrom, P., Garoff, H. et al. (1993) Expression of heterologous proteins in cultured rat hippocampal neurons using the Semliki Forest virus vector. Journal of Neuroscience Research, 35, 445-451.
12. Ulmanen, I., Peranen, J., Tenhunen, J. et al. (1997) Expression and intracellular localization of catechol O-methyltransferase in transfected mammalian cells. European Journal of Biochemistry, 243, 452-459.
13. Zhou, X., Berglund, P., Rhodes, G. el al. (1994) Self-replicating Semliki Forest virus RNA as recombinant vaccine. Vaccine, 12, 1510-1514.
14. Zhou, X., Berglund, P., Zhao, H. et al. (1995) Generation of cytotoxic and humoral responses by nonreplicative recombinant Semliki Forest virus. Proceedings of the National Academy of Sciences, USA, 92, 3009-3013.