Recombinant protein production using the Semliki Forest virus expression system

Cytotechnology

Volumn 24, Issue 1, 1997, Pages 65-72

  Blasey, H D ^a   Lundstrom K ^a   Tate S ^b   Bernard, A R ^a  

^a GLAXO INST FOR MOLECULAR BIOLOGY   (Switzerland)

^b GLAXOSMITHKLINE   (United Kingdom)

Author keywords

human cyclooxygenase 2; infection optimisation; Semliki Forest Virus; suspension process; transient expression

Indexed keywords

EID: 0030950873     PISSN: 09209069     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1007974121182     Document Type: Article

References (13)

1. Berglund P, Sjödberg M, Garoff H, Atkins JG, Sheahan BJ and Liljeström P (1993) Semliki Forest Virus expression system: production of infectious recombinant particles. Bio/Technology 11, 916-920.
2. Blasey HD, Isch C and Bernard AR (1995) Cellcube: a new system for large scale growth of adherent cells. Biotechnology Techniques 9, 10, 727-730.
3. Liljeström P (1994) Alphavirus expression systems, current opinion in Biotechnology, 5, 495-500.
4. Liljeström P and Garoff H (1991a) A new generation of animal cell expression vectors based on the Semliki Forest Virus system. Bio/Technology 9, 1356-1361.
5. Liljeström and Garoff H (1991b) Current protocols. Mol. Biol. 2, 16-20.
6. Lundström K, Mills A, Buell G, Allet E, Adami N and Liljeström P (1994) High-level expression of the human neurokine-1 receptor in mammalian cell lines using the Semliki Forest Virus expression system. Eur. J. Biochem. 244, 917-921.
7. Lee SH, Soyoola E, Chanmugam P, Hart S, Sun W, Zhong H, Liou S, Simmons D and Hwang D (1992) Selective expression of mitogen-inducible cyclooxygenase in macrophages stimulated with lipopolysaccharide. J. Biol. Chem. 26, 25934-25938.
8. Lundström K, Vargas A and Allet B (1995) Functional activity of a biotinylated human neurokinin 1 receptor fusion expressed in the Semliki Forest Virus system. Biochem. Biophys. Res. Comm. 208, 1, 260-266.
9. Mikus P, Urano T, Liljeström P and Ny T (1993) Plasminogen activator inhibitor type 2 (PAI-2) is a spontaneously polymerising SEPRIN: biochemical characterisation of the recombinant intracellular and extracellular form. Eur. J. Biochem. 218, 1071-1082.
10. Olkkonen VM, Liljeström P, Garoff H, Simons K and Dotti C (1993) Expression of heterologous proteins in cultured rat hippocampal neurons using the Semliki Forest Vector. J. Neurosci. Res. 35, 445-451.
11. Paul NL, Marsh M, McKeating JA, Schulz TF, Liljeström P, Garoff H and Weiss RA (1993) Expression of HIV-1 envelope glycoproteins by Semliki Forest Virus Vectors. AIDS Res. Hum. Retroviruses 9, 963-970.
12. Pritchard K, O'Banion MK, Miano J, Vlasic N, Bhatia U, Young D and Stemerman M (1994) Induction of Cyclooxygenase-2 in rat vascular smooth muscle cells in vitro and in vivo. J. Biol. Chem. 269, 8504-8509.
13. Xie W, Robertson DL and Simmons DL (1992) Mitogen-inducible prostaglandin G/H Synthase: a new target for nonsteroidal antiinflammatory drugs. Drug. Dev. Res. 25, 249-265.