Nuclear pores and macromolecular assemblies involved in nucleocytoplasmic transport

Current Opinion in Structural Biology

Volumn 6, Issue 2, 1996, Pages 162-165

  Stewart, Murray ^a   Clarkson, W David ^a  

^a MRC LABORATORY OF MOLECULAR BIOLOGY   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

CARRIER PROTEIN; CYTOPLASM PROTEIN; NUCLEOPORIN;

CELL NUCLEUS; CELL NUCLEUS MEMBRANE; COMPLEX FORMATION; CYTOPLASM; INTRACELLULAR TRANSPORT; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION; PROTEIN TRANSPORT; SHORT SURVEY;

EID: 0029981666     PISSN: 0959440X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0959-440X(96)80070-4     Document Type: Article

References (43)

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14. r 45 kDa, 54 kDa, 58 kDa and 62 kDa in equal proportions. The molecular weight of the intact complex indicates that probably one chain of each protein is present in the complex, and electron microscopy indicates that these particles have a doughnut-like shape that is about 15 nm in diameter.
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27. + RNA export and is postulated to tether nucleoporins directly involved in transport to pore scaffolding proteins via its coiled-coil domain.
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38. Radu A, Blobel G, Moore M: Identification of a protein complex that is required for nuclear protein import and mediates docking of import substrate to distinct nucleoporins. Proc Natl Acad Sci USA 1995, 92:1760-1773. A 9S protein complex from Xenopus cytosol containing 54 kDa, 56 kDa and 97 kDa proteins was isolated. This complex promoted the binding of a NLS-containing ligand to four nucleoporins and suggested that these, possibly together with other nucleoporins, act as multiple docking sites for importin-mediated transport of NLS-containing substrates through the NPC.
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40. Kent HM, Clarkson WD, Bullock TM, Stewart M: Crystallization and preliminary X-ray diffraction analysis of nuclear transport factor 2 (NTF2). J Struct Biol 1996, in press. This paper reports the production of NTF2 crystals that should enable their high-resolution structure to be determined.
41. Wu J, Matunis MJ, Kraemer D, Blobel G, Coutavas E: Nup358, a cytoplasmically exposed nucleoporin with peptide repeats, Ran-GTP binding sites, zinc fingers, a cyclophilin A homologous domain, and a leucine rich region. J Biol Chem 1995, 270:14209-14213. This paper describes the identification and characterization of a NPC Ran-binding protein that contains a wealth of different sequence motifs, outlined in the title (see also [42•]).
42. Yokoyama N, Hayashi N, Seki T, Pante N, Ohba T, Nishii K, Kuma K, Hayashida T, Miyata T, Aebi U et al.: A giant nucleopore protein that binds Ran/TC4. Nature 1995, 376:184-188. A 700-residue nucleoporin that binds Ran was identified using a two-hybrid screen. This protein binds to the cytoplasmic surface of the NPC and contains a range of different repeating sequence motifs including eight zinc-finger motifs and a leucine-zipper as well as a putative cyclophilin-binding domain (see also [41•]).
43. Hartmann E, Görlich D: A Ran-binding motif in nuclear pore proteins. Trends Cell Biol 1995, 5:192-193. Several nucleoporins contain a consensus Ran-binding motif.