Towards minimized gonadotropins with full bioactivity

European Journal of Biochemistry

Volumn 261, Issue 1, 1999, Pages 81-84

  Heikoop, Judith C ^b   Huisman De Winkel, Bianca ^b   Grootenhuis, Peter D J ^a  

^a CombiChem ^*  (United States)

^b NONE

Author keywords

Gonadotropin; Human choriogonadotropin; Protein minimization; Single chain

Indexed keywords

CHORIONIC GONADOTROPIN; CHORIONIC GONADOTROPIN RECEPTOR; GLYCOPROTEIN; GONADOTROPIN; LUTEINIZING HORMONE RECEPTOR;

ALPHA CHAIN; ANIMAL CELL; ARTICLE; CHO CELL; CONTROLLED STUDY; GENETIC ENGINEERING; HORMONE ACTION; HORMONE RECEPTOR INTERACTION; HORMONE STRUCTURE; MOLECULAR SIZE; MOLECULAR WEIGHT; NONHUMAN; PRIORITY JOURNAL; PROTEIN GLYCOSYLATION; RECEPTOR AFFINITY;

EID: 0013663164     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.1999.00232.x     Document Type: Article

References (14)

1. 1. Combarnous, Y. (1992) Molecular basis of the specificity of binding of glycoprotein hormones to their receptors. Endocrin. Rev. 13, 670-691.
2. 2. Lapthorn, A.J., Harris, D.C., Littlejohn, A., Lustbader, J.W., Canfield, R.E., Machin, K.J., Morgan, F.J. & Isaacs, N.W. (1994) Crystal structure of human chorionic gonadotropin. Nature 369, 455-461.
3. 3. Wu, H., Lustbader, J.W., Liu, Y., Canfield, R.E. & Hendrickson, W.A. (1994) Structure of human chorionic gonadotropin at 2.6 Å resolution from MAD analysis of the selenomethionyl protein. Structure 2, 545-558.
4. 4. Cunningham, C.C. & Wells, J.A. (1997) Minimized proteins. Curr. Opin. Struct. Biol. 7, 457-462.
5. 5. Braisted, A.C. & Wells, J.A. (1996) Minimizing a binding domain from protein A. Proc. Natl Acad. Sci. USA 93, 5688-5692.
6. 6. Li, B., Tom, J.Y., Oare, D., Yen, R., Fairbrother, W.J., Wells, J.A. & Cunningham, B.C. (1995) Minimization of a polypeptide hormone. Science 270, 1657-1660.
7. 7. Yanofsky, S.D., Baldwin, D.N., Butler, J.H., Holden, F.R., Jacobs, J.W., Balasubramanian, P., Chinn, J.P., Cwirla, S.E., Peters-Bhatt, E., Whitehorn, E.A., Tate, E.H., Akeson, A., Bowlin, T.L., Dower, W.J. & Barrett, R.W. (1996) High affinity type I interleukin 1 receptor antagonists discovered by screening recombinant peptide libraries. Proc. Natl Acad. Sci. USA 93, 7381-7386.
8. 8. Wrighton, N.C., Farrell, F.X., Chang, R., Kashyap, A.K., Barbone, F.P., Mulcahy, L.S., Johnson, D.L., Barrett, R.W., Jolliffe, L.K. & Dower, W.J. (1996) Small peptides as potent mimetics of the protein hormone erythropoietin. Science 273, 458-463.
9. 9. Heikoop, J.C., van Beuningen-de Vaan, M.J.A.C.M., van den Boogaart, P. & Grootenhuis, P.D.J. (1997) Evaluation of subunit truncation and the nature of the spacer for single-chain gonadotropins. Eur. J. Biochem. 245, 656-662.
10. 10. Szkudlinski, M.W., The, N.G., Grossmann, M., Tropea, J.E. & Weintraub, B.D. (1996) Engineering human glycoprotein hormone superactive analogues. Nature Biotechnol. 14, 1257-1263.
11. 11. Higuchi, R. (1989) Using PCR to engineer DNA. In PCR Technology; Principles and Applications for DNA Amplification (Erlich, H. A., ed.), pp. 61-70. Stockton Press, New York.
12. 12. Olijve, W., de Boer, W., Mulders, J.W.M. & van Wezenbeek, P.M.G.F. (1996) Molecular biology and biochemistry of human recombinant follicle stimulating hormone (Puregon). Mol. Hum. Reprod. 2, 371-382.
13. 13. Bosch, A.M.G., Stevens, W., Schuurs, A., Schonherr, O. & Roelofs, H. (1981) Characteristics of monoclonal antibodies against human chorionic gonadotrophin. In: Protides of the Biological Fluids -Proceedings of the Twenty-Ninth Colloquim (Peeters, H., ed.), pp. 837-842. Pergamon Press, Oxford.
14. 14. Heikoop, J.C., van den Boogaart, P., Leeuw, R., Rose, U.M., Mulders, J.W.M. & Grootenhuis, P.D.J. (1998) Partially deglycosylated human choriogonadotropin, stabilized by intersubunit disulfide bonds, shows full bioactivity. Eur. J. Biochem. 253, 354-356.