Different conformations of nascent polypeptides during translocation across the ER membrane

BMC Cell Biology

Volumn 1, Issue , 2000, Pages

  Mingarro, Ismael ^b   Nilsson, IngMarie ^a   Whitley, Paul ^c   von Heijne, Gunnar ^a  

^a STOCKHOLM UNIVERSITY   (Sweden)

^b UNIVERSITY OF VALENCIA   (Spain)

^c University of Bath   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ALANINE; AMINO ACID; ASPARAGINE; GLYCAN; LEUCINE; POLYPEPTIDE; PROLINE; SERINE; THREONINE; TRANSFERASE; VALINE;

ALPHA HELIX; AMINO TERMINAL SEQUENCE; ANIMAL CELL; ARTICLE; CARBOXY TERMINAL SEQUENCE; COMPLEX FORMATION; CONTROLLED STUDY; ENDOPLASMIC RETICULUM; ENZYME ACTIVE SITE; INTRACELLULAR TRANSPORT; MEMBRANE TRANSPORT; MOLECULAR PROBE; NONHUMAN; PROTEIN CONFORMATION; PROTEIN FOLDING; PROTEIN GLYCOSYLATION; PROTEIN TRANSPORT; STOP CODON; TRANSPORT KINETICS;

ANIMALIA; EUKARYOTA;

EID: 0012249596     PISSN: 14712121     EISSN: None     Source Type: Journal    
DOI: 10.1186/1471-2121-1-3     Document Type: Article

References (24)

1. Johnson AE, van Waes MA: The translocon: A dynamic gateway at the ER membrane. Annual Review of Cell and Developmental Biology 1999, 15:799-842
2. Nissen P, Hansen J, Ban N, Moore P, Steitz T: The structural basis of ribosome activity in protein synthesis. Science 2000, 289:920-930
3. Morgan D, Ménétret J-F, Radermacher M, Neuhof A, Akey I, Rapoport T, Akey C: A comparison of the yeast and rabbit 80 S ribosome reveals the topology of the nascent chain exit tunnel, inter-subunits bridges and mammalian rRNA expansion segments. J Mol Biol 2000, 301:301-321
4. Hamman B, Chen J-C, Johnson E, Johnson A: The aqueous pore through the translocon has a diameter of 40-60 Å during cotrsnaltional protein translocation at the ER membrane. Cell 1997, 89:535-544
5. Tsalkova T, Odom O, Kramer G, Hardesty B: Different conformations of nascent peptides on ribosomes. J Mol Biol 1998, 278:713-723
6. Makeyev EV, Kolb VA, Spirin AS: Enzymatic activity of the ribosome bound nascent polypeptide. FEBS Lett 1996, 378:166-170
7. Netzer WJ, Hartl FU: Recombination of protein domains facilitated by co- translational folding in eukaryotes. Nature 1997, 388:343-349
8. MacKenzie KR, Prestegard JH, Engelman DM: A transmembrane helix dimer: Structure and implications. Science 1997, 276:131-133
9. Li S-C, Deber CM: A measure of helical propensity for amino acids in membrane environments. Nature Struct Biol 1994, 1:368-373
10. Whitley P, Nilsson IM, von Heijne G: A nascent secretory protein may traverse the ribosome/ER translocase complex as an extended chain. J Biol Chem 1996, 271:6241-6244
11. Nilsson I, von Heijne G: Glycosylation efficiency of Asn-Xaa-Thr sequons depends both on the distance from the C terminus and on the presence of a downstream transmembrane segment. J Biol Chem 2000, 275:17338-17343
12. Stapley B, Creamer T: A survey of left-handed polyproline II helices. Protein Sci 1999, 8:587-595
13. von Heijne G: Recent advances in the understanding of membrane protein assembly and structure. Quart Rev Biophys 2000, 32:285-307
14. Nilsson I, Sääf A, Whitley P, Gafvelin G, Waller C, von Heijne G: Proline-induced disruption of a transmembrane α-helix in its natural environment. J Mol Biol 1998, 284:1165-1175
15. Monné M, Nilsson I, Johansson M, Elmhed N, von Heijne G: Positively and negatively charged residues have different effects on the position in the membrane of a model transmembrane helix. J Mol Biol 1998, 284:1177-1183
16. Armulik A, Nilsson I, von Heijne G, Johansson S: Determination of the border between transmembrane and cytoplasmic domains of human integrin subunits. J Biol Chem 1999, 274:37030-37034
17. Levitt M: Conformational preferences of amino acids in globular proteins. Biochemistry 1978, 17:4277-4285
18. Kuroiwa T, Sakaguchi M, Mihara K, Omura T: Systematic analysis of stop-transfer sequence for microsomal membrane. J Biol Chem 1991, 266:9251-9255
19. Heinrich S, mothes W, Brunner J, Rapoport T: The Sec61p complex mediates the integration of a membrane protein by allowing lipid partitioning of the transmembrane domain. Cell 2000, 102:233-244
20. Kunkel TA: Rapid and efficient site-specific mutagenesis without phenotypic selection. Methods Enzymol. 1987, 154:367-382
21. Geisselsoder J, Witney F, Yuckenberg P: Efficient site-directed in vitro mutagenesis. BioTechniques 1987, 5:786-791
22. Kozak M: Initiation of translation in prokaryotes and eukaryotes. Gene 1999, 234:187-208
23. Liljeström P, Garoff H: Internally located cleavable signal sequences direct the formation of Semliki Forest virus membrane proteins from a polyprotein precursor. J Virol 1991, 65:147-154
24. Sääf A, Wallin E, von Heijne G: Stop-transfer function of pseudorandom amino acid segments during translocation across prokaryotic and eukaryotic membranes. Eur J Biochem 1998, 251:821-829