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Moscat J and Aaronson SA: Novel Source of 1,2diacylglicerol Elevated in Cells Transformed by Ha-ras Oncogene
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(1993) 8:2959-2968. • This paper provided the first time demonstration of the mitogenic activity of PCho, establishing a link between growth factor- and oncogene-induced cell proliferation. The study also provided the basis for oncogene-driven transformation as a consequence of an unbalance of lipid metabolites
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Cuadrado A, Camera A, Dolfi F, Jiménez B, Lacal JC. Phosphorylcholine: a novel second messenger essential for mitogenic activity of growth factors. Oncogene (1993) 8:2959-2968. • This paper provided the first time demonstration of the mitogenic activity of PCho, establishing a link between growth factor- and oncogene-induced cell proliferation. The study also provided the basis for oncogene-driven transformation as a consequence of an unbalance of lipid metabolites:
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It demonstrates that non-tumorigenic cells are less sensitive to choline kinase inhibitors than their rastranstomned counterparts.
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Hemandez-Alcoceba R, Saniger L, Campos J, Nunez MC, Khaless F, Gallo MA, Espinosa A, Lacal JC: Choline kinase inhibitors as a novel approach for antiproliferatvie drug design. Oncogene (1997) 15:2289-2301. •• This paper provided solid experimental evidence for the possible use of choline kinase inhibitors as novel antitumor drugs. It demonstrates that non-tumorigenic cells are less sensitive to choline kinase inhibitors than their rastranstomned counterparts.
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Saniger L, Campos J, Nunez MC, Khaless F, Gallo MA, Espinosa A, Lacal JC: Choline Kinase Inhibitors As A Novel Approach for Antiproliferatvie Drug Design. Oncogene (1997) 15:2289-2301. ••
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Uchida T: Stimulation of phospholipid synthesis in HeLa cells by epidermal growth factor and insulin: activation of choline kinase and glycerophosphate acyltransferase. Biochim Biophys Acta (1996) 1304(2):89-104.
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Uchida T: Immunologically and enzymatically distinct rat choline kinase isozymes. J Biochem (1994) 116(6):1241-1250.
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• This manuscript demonstrates a dear correlation between an increase in PCho levels and transformation in human tumors. Furthermore, it demonstrates that this is independent of cell proliferation status. It establishes solid grounds for the analysis, by NMR, of the levels of PCho in human tumors as a valid diagnostic strategy to correlate with the transforming phenotype.
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Bhakoo KK, ' Williams SR, Florian CL, Und H, Noble M: Immortalization and transformation are associated with specific alterations in choline metabolism. Cancer Res (1996) 56:4630-4635. • This manuscript demonstrates a dear correlation between an increase in PCho levels and transformation in human tumors. Furthermore, it demonstrates that this is independent of cell proliferation status. It establishes solid grounds for the analysis, by NMR, of the levels of PCho in human tumors as a valid diagnostic strategy to correlate with the transforming phenotype.
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' Williams SR, Florian CL, und H, Noble M: Immortalization and Transformation Are Associated with Specific Alterations in Choline Metabolism. Cancer Res (1996) 56:4630-4635.
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• This work demonstrates that choline kinase is elevated both in terms of enzyme amounts and activity in human colorectal tumors. However, no correlation with the specific stage of the tumor or its prognosis is reported.
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Nakagami K, Uchida T, Ohwada S, Koibuchi Y, Suda Y, Sekine T, Morishita Y: Increased choline kinase activity and elevated phosphocholine levels in human colon cancer. Jpn J Cancer Res (1999)90:419-124. • This work demonstrates that choline kinase is elevated both in terms of enzyme amounts and activity in human colorectal tumors. However, no correlation with the specific stage of the tumor or its prognosis is reported.
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Uchida T, Ohwada S, Koibuchi Y, Suda Y, Sekine T, Morishita Y: Increased Choline Kinase Activity and Elevated Phosphocholine Levels in Human Colon Cancer. Jpn J Cancer Res (1999)90:419-124.
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Uchida T, Ohwada S, Koibuchi Y, Morishita Y: Increased Choline Kinase Activity in 1,2-dimethylhydrazine-induced Rat Colon Cancer.
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Excellent review that provides full information on the structure and properties of the choline kinase inhibitor hemicholinium-3 and its derivatives.
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Cannon JG: Structure-activity aspects of hemicholinium-3 (HC-3) and its analogues and congeners. Med Res Rev (1994) 14(5):505531. Excellent review that provides full information on the structure and properties of the choline kinase inhibitor hemicholinium-3 and its derivatives.
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Cannon, J.G.1
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Campos J, Nünez MC, Rodriguez V, Gallo MA, Espinosa A: QSAR of 1,V-(1,2-ethylenebisbenzyl)bis{4-substituted pyridinium) dibromides as choline kinase inhibitors: a different approach for antiproliferative drug design. Biorg Med Chem (2000) 10(8):767-770.
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Nünez MC, Rodriguez V, Gallo MA, Espinosa A: QSAR of 1,V-(1,2-ethylenebisbenzyl)bis{4-substituted Pyridinium Dibromides As Choline Kinase Inhibitors: A Different Approach for Antiproliferative Drug Design.
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Campos J, Nunez MC, Rodriguez V, Entrena A, Gallo MA, HemândezAlcoceba R, Femândez F, Laçai JC, Espinosa A: LU MO Energy of model compounds as an index for the inhibition of choline kinase: chemical meaning. Eur J Med Chem (2001) In press.
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Nunez MC, Rodriguez V, Entrena A, Gallo MA, HemândezAlcoceba R, Femândez F, Laçai JC, Espinosa A: LU MO Energy of Model Compounds As An Index for the Inhibition of Choline Kinase: Chemical Meaning.
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