Enzyme-mediated sulfide production for the reconstitution of [2Fe-2S] clusters into apo-biotin synthase of Escherichia coli. Sulfide transfer from cysteine to biotin

European Journal of Biochemistry

Volumn 267, Issue 9, 2000, Pages 2688-2694

  Tse Sum Bui, Bernadette ^a   Escalettes, Franck ^a   Chottard, Geneviève ^b   Florentin, Dominique ^a   Marquet, Andrée ^a  

^a Centre National de la Recherche Scientifique   (France)

^b EUROPEAN SPACE AGENCY   (France)

Author keywords

(seleno) biotin synthase; Iron sulfur (selenium) cluster; NifS; Sulfur transfer

Indexed keywords

APOENZYME; BIOTIN SYNTHASE; CYSTEINE; IRON SULFUR PROTEIN; SELENIUM; SELENOCYSTINE; SULFIDE; THIOSULFATE; THIOSULFATE SULFURTRANSFERASE;

ARTICLE; AZOTOBACTER VINELANDII; CYANOBACTERIUM; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME MECHANISM; ENZYME METABOLISM; ENZYME RECONSTITUTION; ESCHERICHIA COLI; NONHUMAN; PRIORITY JOURNAL; SULFATION;

BACTERIAL PROTEINS; BIOTIN; CYSTEINE; ESCHERICHIA COLI; IRON-SULFUR PROTEINS; SULFIDES; SULFURTRANSFERASES;

AZOTOBACTER VINELANDII; BOVINAE; ESCHERICHIA COLI; SYNECHOCYSTIS;

EID: 0000638382     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.2000.01284.x     Document Type: Article

References (47)

1. 1. Sanyal, I., Cohen, G. & Flint, D.H. (1994) Biotin synthase: Purification, characterization as a [2Fe-2S] cluster protein and in vitro activity of the Escherichia coli bioB gene product. Biochemistry 33, 3625-3631.
2. 2. Méjean, A., Tse Sum Bui, B., Florentin, D., Ploux, O., Izumi, Y. & Marquet, A. (1995) Highly purified biotin synthase can transform dethiobiotin into biotin in the absence of any other protein, in the presence of photoreduced deazaflavin. Biochem. Biophys. Res. Commun. 217, 1231-1237.
3. 3. Tse Sum Bui, B., Florentin, D., Fournier, F., Ploux, O., Méjean, A. & Marquet, A. (1998) Biotin synthase mechanism: on the origin of sulphur. FEBS Lett. 440, 226-230.
4. 4. Duin, E.C., Lafferty, M.E., Crouse, B.R., Allen, R.M., Sanyal, I., Flint, D.H. & Johnson, M.K. (1997) [2Fe-2S] to [4Fe-4S] cluster conversion in Escherichia coli biotin synthase. Biochemistry 36, 11811-11820.
5. 2+ clusters. FEBS Lett. 459, 411-414.
6. 6. Ifuku, O., Kishimoto, J., Haze, S., Yanagi, M. & Fukushima, S. (1992) Conversion of dethiobiotin to biotin in cell-free extracts of Escherichia coli. Biosci. Biotech. Biochem. 56, 1780-1785.
7. 7. Florentin, D., Tse Sum Bui, B., Marquet, A., Ohshiro, T. & Izumi, Y. (1994) On the mechanism of biotin synthase of Bacillus sphaericus. C.R. Acad. Sci. Paris, Life Sci. 317, 485-488.
8. 8. Ifuku, O., Koga, N., Haze, S., Kishimoto, J. & Wachi, Y. (1994) Flavodoxin is required for conversion of dethiobiotin to biotin in Escherichia coli. Eur. J. Biochem. 224, 173-178.
9. 9. Birch, O.M., Fuhrmann, M. & Shaw, N.M. (1995) Biotin synthase from Escherichia coli, an investigation of the low molecular weight and protein components required for activity in vitro. J. Biol. Chem. 270, 19158-19165.
10. 10. Sanyal, I., Gibson, K.J. & Flint, D.H. (1996) Escherichia coli biolin synthase: an investigation into the factors required for its activity and its sulfur donor. Arch. Biochem. Biophys. 326, 48-56.
11. 11. Guianvarc'h, D., Florentin, D., Tse Sum Bui, B., Nunzi, F. & Marquet, A. (1997) Biotin synthase, a new member of the family of enzymes which uses S-adenosylmethionine as a source of deoxyadenosyl radical. Biochem. Biophys. Res. Commun. 236, 402-406.
12. 12. Escalettes, F., Florentin, D., Tse Sum Bui, B., Lesage, D. & Marquet, A. (1999) Biotin synthase mechanism: evidence for hydrogen transfer from the substrate into deoxyadenosine. J. Am. Chem. Soc. 121, 3571-3578.
13. 13. Jacobson, M.R., Cash, V.L., Weiss, M.C., Laird, N.F., Newton, W.E. & Dean, D.R. (1989) Biochemical and genetic analysis of the nifUSVWZM cluster from Azotobacter vinelandii. Mol Gen. Genet. 219, 49-57.
14. 14. Zheng, L., White, R.H., Cash, V.L. & Dean, D.R. (1994) Mechanism for the desulfurization of L-cysteine catalyzed by the nifS gene product. Biochemistry 33, 4714-4720.
15. 15. Zheng, L. & Dean, D.R. (1994) Catalytic formation of a nitrogenase iron-sulfur cluster. J. Biol. Chem. 269, 18723-18726.
16. 16. Fu, W., Jack, R.F., Morgan, TV, Dean, D.R. & Johnson, M.K. (1994) NifU gene product from Azotobacter vinelandii is a homodimer that contains two identical [2Fe-2S] clusters. Biochemistry 33, 13455-13463.
17. 17. Hidalgo, E. & Demple, B. (1996) Activation of SoxR-dependent transcription in vitro by non-catalytic or NifS-mediated assembly of [2Fe-2S] clusters into apo-SoxR. J. Biol. Chem. 271, 7269-7272.
18. 2: [4Fe-4S] to [2Fe-2S] conversion with loss of biological activity. Proc. Natl Acad. Sci. USA 94, 6087-6092.
19. 19. Flint, D.H. (1996) Escherichia coli contains a protein that is homologous in function and N-terminal sequence to the protein encoded by the nifS gene of Azotobacter vinelandii and that can participate in the synthesis of the Fe-S cluster of dihydroxy-acid dehydratase. J. Biol. Chem. 271, 16068-16074.
20. 20. Leibrecht, I. & Kessler, D. (1997) A novel L-cysteine/cystine C-S-lyase directing [2Fe-2S] cluster formation of Synechocystis ferredoxin. J. Biol. Chem. 272, 10442-10447.
21. 21. Westley, J. (1981) Thiosulfate : cyanide sulfurtransferase (rhodanese). Methods Enzymol. 11, 285-291.
22. 22. Ohshiro, T., Yamamoto, M., Tse Sum Bui, B., Florentin, D., Marquet, A. & Izumi, Y. (1995) Stimulatory factors for enzymatic biotin synthesis from dethiobiotin in cell-free extracts of Escherichia coli. Biosci. Biotech. Biochem. 59, 943-944.
23. 23. Zheng, L., White, R.H., Cash, V.L., Jack, R.F. & Dean, D.R. (1993) Cysteine desulfurase activity indicates a role for NifS in metallo-cluster biosynthesis. Proc. Natl Acad. Sci. USA 90, 2754-2758.
24. 24. Bradford, M.M. (1976) A rapid and sensitive method for the quantification of microgram quantities of protein using the principle of protein-dye binding. Anal. Biochem. 72, 248-254.
25. 25. Fish, W.W. (1988) Rapid colorimetric micromethod for the quantitation of complexed iron in biological samples. Methods Enzymol. 158, 357-364.
26. 26. Beinert, H. (1983) Semi-micro methods for analysis of labile sulfide and of labile sulfide plus sulfane sulfur in unusually stable iron-sulfur proteins. Anal. Biochem. 131, 373-378.
27. 27. Izumi, Y, Osakai, M. & Yamada, H. (1987) Biotin assay using lyophilized and glycerol-suspended cultures. J. Microbiol. Methods 6, 237-246.
28. 28. Miller, D.M., Kurzban, G.P., Mendoza, J.A., Chirgwin, J.M., Hardies, S.C. & Horowitz, P.M. (1992) Recombinant bovine rhodanese: purification and comparison with bovine liver rhodanese. Biochim. Biophys. Acta 1121, 286-292.
29. 29. Fee, J.A. & Palmer, G. (1971) The properties of parsley ferredoxin and its selenium-containing homolog. Biochem. Biophys. Acta 245, 175-195.
30. 30. Meyer, J., Moulis, J.-M., Gaillard, J. & Lutz, M. (1992) Replacement of sulfur by selenium in iron-sulfur proteins. Adv. Inorg. Chem. 38, 73-115.
31. 31. Alexander, K. & Volini, M. (1987) Properties of an Escherichia coli rhodanese. J. Biol. Chem. 262, 6595-6604.
32. 32. Pecci, L., Pensa, B., Costa, M., Cignini, P.L. & Canella, C. (1976) Reaction of rhodanese with dithiothreitol. Biochim. Biophys. Acta 445, 104-111.
33. 33. Bonomi, F., Pagani, S., Cerletti, P. & Cannella, C. (1977) Rhodanese-mediated sulfur transfer to succinate dehydrogenase. Eur. J. Biochem. 72, 17-24.
34. 34. Bonomi, F., Pagani, S. & Kurtz, D.M. Jr (1985) Enzymic synthesis of the 4Fe-4S clusters of Clostridium pasteurianum ferredoxin. Eur. J. Biochem. 148, 67-73.
35. 35. Pagani, S., Eldridge, M. & Eady, R.R. (1987) Nitrogenase of Klebsiella pneumoniae, rhodanese-catalysed restoration of activity of the inactive 2Fe species of the Fe protein. Biochem. J. 244, 485-488.
36. 36. DeMoll, E. & Shive, W. (1983) The origin of sulfur in biotin. Biochem. Biophys. Res. Commun. 110, 243-249.
37. 37. DeMoll, E., White, R.H. & Shive, W. (1984) Determination of the metabolic origins of the sulfur and 3′-nitrogen atoms in biotin of Escherichia coli by mass spectrometry. Biochemistry 23, 558-562.
38. 38. Gibson, K.J., Pelletier, D.A. & Turner, I.M. Sr, (1999) Transfer of sulfur to biotin from biotin synthase (BioB protein). Biochem. Biophys. Res. Commun. 254, 632-635.
39. 35S]sulfide into the Fe-S cluster of aconitase. J. Biol. Chem. 259, 3145-3151.
40. 40. Hong, J.-S. & Rabinowitz, J.C. (1970) Base-catalyzed exchange of the iron and sulfide of clostridial ferredoxin. J. Biol. Chem. 245, 6582-6587.
41. 41. Mihara, H., Kurihara, T., Yoshimura, T., Soda, K. & Esaki, N. (1997) Cysteine sulfinate desulfinase, a NifS-like protein of Escherichia coli with selenocysteine lyase and cysteine desulfurase activities. J. Biol. Chem. 272, 22417-22424.
42. 42. Lang, T. & Kessler, D. (1999) Evidence for cysteine persulfide as reaction product of L-cyst(e)ine C-S-Lyase (C-DES) from Synechocystis. J. Biol. Chem. 274, 189-195.
43. 43. Blattner, F.R., Plunkett, G., Bloch, C.A., Perna, N.T., Burland, V. Riley, M., Collado-Vides, J., Glasner, J.D., Rode, C.K., Mayhew, G.F., Gregor, J., Davis, N.W., Kirkpatrick, H.A., Goeden, M.A., Rose, D.J., Man, B. & Shao, Y. (1997) The complete genome sequence of Escherichia coli K-12. Science 277, 1453-1462.
44. 44. Zheng, L., Cash, V.L., Flint, D.H. & Dean, D.R. (1998) Assembly of iron-sulfur clusters. J. Biol. Chem. 273, 13264-13272.
45. 45. Jung, Y.S., Gao-Sheridan, H.S., Christiansen, J., Dean, D.R. & Burgess, B.K. (1999) Purification and biological characterization of a new [2Fe-2S] ferredoxin from Azotobacter vinelandii, a putative [Fe-S] cluster assembly/repair protein. J. Biol. Chem. 274, 32402-32410.
46. 46. Fleischmann, R.D., Adams, M.D., White, O., Clayton, R.A., Kirkness, E.F., Kerlavage, A.R., Bult, C.J., Tomb, J.-F, Dougherty, B.A., Merrick, J.M., McKenny, K., Sutton, G., FitzHugh, W., Fields, C., Gocayne, J.D., Scott, J.D., Shirley, R., Liu, L.-I., Glodek, A., Kelley, J.M., Weidman, J.F., Phillips, C.A., Spriggs, T., Hedhlom, E., Cotton, M.D., Utterback, T.R., Hanna, M.C., Nguyen, D.T., Saudek, D.M., Brandon, R.C., Fine, L.D., Fritchman, J.L., Fuhrmann, J.L., Geoghagen, N.S.M., Gnehm, C.L., McDonald, L.A., Small, K.V., Fraser, C.M., Smith, H.O. & Venter, J.C. (1995) Whole-genome random sequencing and assembly of Haemophilus influenzae RD. Science 269, 496-512.
47. 47. Takahashi, Y. & Nakamura, M. (1999) Functional assignment of the ORF2-iscS-iscU-iscA-hscB-hscA-fdx-ORf3 gene cluster involved in the assembly of Fe-S clusters in Escherichia coli. J. Biochem. 126, 917-926.