Reconstitution of nuclear pore assembly and function

Seminars in Cell and Developmental Biology

Volumn 7, Issue 4, 1996, Pages 475-486

  Macaulay, Colin ^a   Forbes, Douglass J ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Membrane; Nuclear transport; Nucleoporin; Pore

Indexed keywords

EID: 0000450002     PISSN: 10849521     EISSN: None     Source Type: Journal    
DOI: 10.1006/scdb.1996.0060     Document Type: Article

References (107)

1. Goldberg MW, Allen TD (1996) The nuclear pore complex and lamina - three-dimensional structures and interactions determined by field emission in-lens scanning electron microscopy. J Mol Biol 257:848-865
2. Pante N, Aebi U (1994) Toward the molecular details of the nuclear pore complex. J Struct Biol 113:179-189
3. Akey CW, Radermacher M (1993) Architecture of the Xenopus nuclear pore complex revealed by three-dimensional cryo-electron microscopy. J Cell Biol 122:1-19
4. Goldberg MW, Blow JJ, Allen TD (1992) The use of field emission in-lens scanning electron microscopy to study the steps of assembly of the nuclear envelope in vitro. J Struct Biol 108:257-268
5. Hinshaw JE, Carragher BO, Milligan RA (1992) Architecture and design of the nuclear pore complex. Cell 69:1133-1141
6. Ris H (1991) The three-dimensional structure of the nuclear pore complex as seen by high voltage electron microscopy and high resolution low voltage scanning electron microscopy. EMSA Bull 21:54-56
7. Forbes DJ (1992) Structure and function of the nuclear pore complex. Annu Rev Cell Biol 8:495-527
8. Rout MP, Wente SR (1994) Pores for thought: nuclear pore complex proteins. Trends Cell Biol 4:357-365
9. Goldberg MW, Allen TD (1995) Structural and functional organization of the nuclear envelope. Curr Opin Cell Biol 7:301-309
10. Davis L (1995) The nuclear pore complex. Annu Rev Biochem 64:865-896
11. Rout MP, Blobel G (1993) Isolation of the yeast nuclear pore complex. J Cell Biol 123:771-783
12. Strambio-de-Castillia C, Blobel G, Rout MP (1995) Isolation and characterization of nuclear envelopes from the yeast Saccharomyces. J Cell Biol 131:19-31
13. Boulikas T (1993) Nuclear localization signals (NLS). Crit Rev Euk Gene Express 3:193-227
14. Newmeyer DD, Forbes DJ (1988) Nuclear import can be separated into distinct steps in vitro: nuclear pore binding and translocation. Cell 52:641-653
15. Richardson WD, Mills AD, Dilworth SM, Laskey RA, Dingwall C (1988) Nuclear protein migration involves two steps: rapid binding at the nuclear envelope followed by slower translocation through nuclear pores. Cell 52:655-664
16. Newmeyer DD, Forbes DJ (1990) An N-ethylmaleimide-sensitive cytosolic factor necessary for nuclear protein import: requirement in signal-mediated binding to the nuclear pore. J Cell Biol 110:547-557
17. Adam SA, Marr RS, Gerace L (1990) Nuclear protein import in permeabilized mammalian cells requires soluble cytoplasmic factors. J Cell Biol 111:807-816
18. Adam EJ, Adam SA (1994) Identification of cytosolic factors required for nuclear location sequence-mediated binding to the nuclear envelope. J Cell Biol 125:547-555
19. Görlich D, Prehn S, Laskey RA, Hartmann E (1994) Isolation of a protein that is essential for the first step of nuclear protein import. Cell 79:767-778
20. Chi NC, Adam EJH, Adam SA (1995) Sequence and characterization of cytoplasmic nuclear protein import factor p97. J Cell Biol 130:265-274
21. Radu A, Blobel G, Moore MS (1995) Identification of a protein complex that is required for nuclear protein import and mediates docking of import substrate to distinct nucleoporins. Proc Nat Acad Sci 92:1769-1773
22. Görlich D, Kostka S, Kraft R, Dingwall C, Laskey RA, Hartmann E, Prehn S (1995) Two different subunits of importin cooperate to recognize nuclear localization signals and bind them to the nuclear envelope. Curr Biol 5:383-392
23. Moroianu J, Blobel G, Radu A (1995) Previously identified protein of uncertain function is karyopherin alpha and together with karyopherin beta docks import substrate at nuclear pore complexes. Proc Natl Acad Sci USA 92:2008-2011
24. Silver PA, Sadler I, Osborne MA (1989) Yeast proteins that recognize nuclear localization sequences. J Cell Biol 109:983-989
25. Yano R, Oakes M, Yamaghishi M, Dodd JA, Nomura M (1992) Cloning and characterization of SRP1, a suppressor of temperature-sensitive RNA polymerase I mutations, in Saccharomyces cerevisiae. Mol Cell Biol 12:5640-5651
26. Görlich D, Vogel F, Mills AD, Hartmann E, Laskey RA (1995) Distinct functions for the two importin subunits in nuclear protein import. Nature 377:246-248
27. Moroianu J, Hijikata M, Blobel G, Radu A (1995) Mammalian karyopherin α1b and α2b heterdomers: α1 or α2 subunit binds nuclear localization signal and β subunit interacts with peptide repeat-containing nucleoporins. Proc Nat Acad Sci 92:6532-6536
28. Moore MS, Blobel G (1993) The GTP-binding protein Ran/TC4 is required for protein import into the nucleus. Nature 365:661-663
29. Melchior F, Paschal B, Evans J, Gerace L (1993) Inhibition of nuclear protein import by nonhydrolyzable analogues of GTP and identification of the small GTPase Ran/TC4 as an essential transport factor. J Cell Biol 123:1649-1659
30. Powers MA, Forbes DJ (1994) Cytosolic factors in nuclear import: what's importin? Cell 79:931-934
31. Moore MS, Blobel G (1994) A G protein involved in nucleocytoplasmic transport: the role of Ran. Trends. Biochem Sci 19:211-216
32. Fabre E, Hurt EC (1994) Nuclear transport. Curr Opin Cell Biol 6:335-342
33. Melchior F, Gerace L (1995) Mechanisms of nuclear protein import. Curr Opin Cell Biol 7:310-318
34. Adam SA (1995) The importance of importin. Trends Cell Biol 5:189-191
35. Simos G, Hurt EC (1995) Nucleocytoplasmic transport: factors and mechanisms. FEBS Lett 369:107-112
36. Sweet DJ, Gerace L (1995) Taking from the cytoplasm and giving to the pore-soluble transport factors in nuclear protein import. Trends Cell Biol 5:444-447
37. Moore MS (1996) Protein translocation-nuclear export -out of the dark. Curr Biol 6:137-140
38. Görlich D, Mattaj IW (1996) Protein kinesis: Nucleocytoplasmic transport. Science 271:1513-1518
39. Rexach M, Blobel G (1995) Protein import into nuclei: Association and dissociation reactions involving transport substrate, transport factors, and nucleoporins. Cell 83:683-692
40. Floer M, Blobel G (1996) The nuclear transport factor karyopherin β binds stoichiometrically to Ran-GTP and inhibits the Ran GTPase activating protein. J Biol Chem 271:5313-5316
41. Lounsbury KM, Richards SA, Perlungher RR, Macara IG (1996) Ran binding domains promote the interaction of Ran with p97/β-karyopherin, linking the docking and translocation steps of nuclear import. J Biol Chem 271:2357-2360
42. Osborne MA, Silver PA (1993) Nucleocytoplasmic transport in the yeast Saccharomyces cerevisiae. Annu Rev Biochem 62:219-254
43. Akey CW (1990) Visualization of transport-related configurations of the nuclear pore transporter. Biophys J 58:341-355
44. Akey CW, Goldfarb DS (1989) Protein import through the nuclear pore complex is a multistep process. J Cell Biol 109:971-982
45. Dworetzky SI, Lanford RE, Feldherr CM (1988) The effects of variations in the number and sequence of targeting signals on nuclear uptake. J Cell Biol 107:1279-1287
46. Lohka MJ, Masui Y (1983) Formation in vitro of sperm pronuclei and mitotic chromosomes induced by amphibian ooplasmic components. Science 220:719-721
47. Lohka MI, Masui Y (1984) Roles of cytosol and cytoplasmic particles in nuclear envelope assembly and sperm pronuclear formation in cell-free preparations from amphibian eggs. J Cell Biol 98:1222-1230
48. Newport JW (1987) Nuclear reconstitution in vitro: stages of assembly around protein-free DNA. Cell 48:205-217
49. Sheehan MA, Mills AD, Sleeman AM, Laskey RA, Blow JJ (1988) Steps in the assembly of replication-competent nuclei in a cell-free system from Xenopus eggs. J Cell Biol 106:1-12
50. Pfaller R, Newport JW (1992) Nuclear envelope assembly following mitosis. Methods Enzymol 219:60-72
51. Smythe C, Newport JW (1991) Systems for the study of nuclear assembly, DNA replication, and nuclear breakdown in Xenopus laevis egg extracts. Methods Cell Biol 35:449-468
52. Newmeyer DD, Wilson KL (1991) Egg extracts for nuclear import and nuclear assembly reactions. Methods Cell Biol 36:607-634
53. Finlay DR, Newmeyer DD, Price TM, Forbes DJ (1987) Inhibition of in vitro nuclear transport by a lectin that binds to nuclear pores. J Cell Biol 104:189-200
54. Holt GD, Snow CM, Senior A, Haltiwanger RS, Gerace L, Hart GW (1987) Nuclear pore complex glycoproteins contain cytoplasmically disposed O-linked N-acetylglucosamine. J Cell Biol 104:1157-1164
55. Hanover JA, Cohen CK, Willingham MC, Park MK (1987) O-linked N-acetylglucosamine is attached to proteins of the nuclear pore. Evidence for cytoplasmic and nucleoplasmic glycoproteins. J Biol Chem 262:9887-9894
56. Davis LI, Blobel G (1986) Identification and characterization of a nuclear pore complex protein. Cell 45:699-709
57. Finlay DR, Forbes DJ (1990) Reconstitution of biochemically altered nuclear pores: transport can be eliminated and restored. Cell 60:17-29
58. Powers MA, Macaulay C, Masiarz F, Forbes DJ (1995) Reconstituted nuclei depleted of a vertebrate GLFG nuclear pore protein, p97, import but are defective in nuclear growth and replication. J Cell Biol 128:721-736
59. Dabauvalle MC, Loos K, Scheer U (1990) Identification of a soluble precursor complex essential for nuclear pore assembly in vitro. Chromosoma 100:56-66
60. Kraemer D, Wozniak RW, Blobel G, Radu A (1994) The human CAN protein, a putative oncogene product associated with myeloid leukemogenesis, is a nuclear pore complex protein that faces the cytoplasm. Proc Nat Acad Sci 91:1519-1523
61. Panté N, Bastos R, McMorrow I, Burke B, Aebi U (1994) Interactions and three-dimensional localization of a group of nuclear pore complex proteins. J Cell Biol 126:603-617
62. Macaulay C, Meier E, Forbes DJ (1995) Differential mototic phosphorylation of proteins of the nuclear pore complex. J Biol Chem 207:254-262
63. Finlay DR, Meier E, Bradley P, Horecka J, Forbes DJ (1991) A complex of nuclear pore proteins required for pore function. J Cell Biol 114:169-183
64. Dabauvalle MC, Benavente R, Chaly N (1988) Monoclonal antibodies to a Mr 68,000 pore complex glycoprotein interfere with nuclear protein uptake in Xenopus oocytes. Chromosoma 97:193-197
65. Grote M, Kubitscheck U, Reichelt R, Peters R (1995) Mapping of nucleoporins to the center of the nuclear pore complex by post-embedding immunogold electron-microscopy. J Cell Sci 108:2963-2972
66. Kita K, Omata S, Horigome T (1993) Purification and characterization of a nuclear pore glycoprotein complex containing p62. J Biochem 113:377-382
67. Buss F, Stewart M (1995) Macromolecular interactions in the nucleoporin p62 complex of rat nuclear pores: Binding of nucleoporin p54 to the rod domain of p62. J Cell Biol 128:251-261
68. Guan T, Müller S, Klier G Panté N, Blevitt JM, Haner M, Paschal B, Aebi U, Gerace L (1995) Structural analysis of the p62 complex, an assembly of O-linked glycoproteins that localizes near the central gated channel of the nuclear pore complex. Mol Biol Cell 6:1591-1603
69. Starr CM, D'Onofrio M, Park MK, Hanover JA (1990) Primary sequence and heterologous expression of nuclear pore glycoprotein p62. J Cell Biol 110:1861-1871
70. Carmo-Fonseca M, Kern H, Hurt EC (1991) Human nucleoporin p62 and the essential yeast nuclear pore protein NSP1 show sequence homology and a similar domain organization. Eur J Cell Biol 55:17-30
71. Grandi P, Doye V, Hurt EC (1993) Purification of NSP1 reveals complex formation with 'GLFG' nucleoporins and a novel nuclear pore protein NIC96. EMBO J 12:3061-3071
72. Siniossoglou S, Wimmer C, Rieger M, Doye V, Tekotte H, Weise C, Emig S, Segref A, Hurt EC (1996) A novel complex of nucleoporins, which includes sec13p and a sec13p homolog, is essential for normal nuclear pores. Cell 84:265-275
73. Miller MW, Hanover JA (1994) Functional nuclear pores reconstituted with beta 14 galactose-modified O-linked N-acetylglucosamine modified glycoproteins. J Biol Chem 269:9289-9297
74. Radu A, Moore MS, Blobel G (1995) The peptide repeat domain of nucleoporin Nup98 functions as a docking site in transport across the nuclear pore complex. Cell 81:215-222
75. Newport JW, Wilson KL, Dunphy WG (1990) A lamin-independent pathway for nuclear envelope assembly. J Cell Biol 111:2247-2259
76. Jenkins H, Hölman T, Lyon C, Lane B, Stick R, Hutchison C (1993) Nuclei that lack a lamina accumulate karyophilic proteins and assemble a nuclear matrix. J Cell Sci 106:275-285
77. Wilson KL, Newport JW (1988) A trypsin-sensitive receptor on membrane vesicles is required for nuclear envelope formation in vitro. J Cell Biol 107:57-68
78. Pfaller R, Smythe C, Newport JW (1991) Assembly/disassembly of the nuclear envelope membrane: cell cycle-dependent binding of nuclear membrane vesicles to chromatin in vitro. Cell 65:209-217
79. Newport JW, Dunphy W (1992) Characterization of the membrane binding and fusion events during nuclear envelope assembly using purified components. J Cell Biol 116:295-306
80. Macaulay C, Forbes DJ (1996) Assembly of the nuclear pore: Biochemically distinct steps revealed with NEM, GTPgS, and BAPTA. J Cell Biol 132:5-20
81. Boman AL, DelannoyMR, Wilson KL (1992) GTP hydrolysis is required for vesicle fusion during nuclear envelope assembly in vitro. J Cell Biol 116:281-294
82. Pfaller R, Newport J (1995) Characterization of the membrane-chromatin interaction using partially purified regulatory enzymes. J Biol Chem 270:19066-19072
83. Boman AL, Taylor TC, Melancon P, Wilson KL (1992) A role for ADP-ribosylation factor in nuclear vesicle dynamics. Nature 358:512-514
84. Sullivan KM, Busa WB, Wilson KL (1993) Calcium mobilization is required for nuclear vesicle fusion in vitro: implications for membrane traffic and IP3 receptor function. Cell 73:1411-1422
85. Philpott A, Leno GH, Laskey RA (1991) Sperm decondensation in Xenopus egg cytoplasm is mediated bynucleoplasmin. Cell 65:569-578
86. Maul GG, Maul HM, Scogna JE, Lieberman GS, Stein GS, Hsu B.YL, Borun TW (1972) Time sequence of nuclear pore formation in phytohemagglutinin-stimulated lymphocytes and in HeLa cells during the cell cycle. J Cell Biol 55:433-447
87. Morgan A, Burgoyne RD (1995) Is NSF a fusion protein? Trends Cell Biol 5:335-338
88. Confalonieri F, Duguet M (1995) A 200-amino acid ATPase module in search of a basic function. Bioessays 17:639-650
89. Rothman JE, Wieland FT (1996) Protein sorting by transport vesicles. Science 272:227-234
90. Denesvre C, Malhotra V (1996) Membrane fusion in organelle biogenesis. Curr Opin Cell Biol 8:in press
91. Peters JM, Walsh MJ, Franke WW (1990) An abundant and ubiquitous homo-oligomeric ring-shaped ATPase particle related to the putative vesicle fusion proteins Sec18p and NSF. EMBO J 9:1757-1767
92. Boman AL, Kahn RA (1995) ARF proteins: the membrane traffic police? Trends Biochem Sci 20:147-150
93. Tsien RY (1980) New calcium indicators and buffers with high selectivity against magnesium and protons: design, synthesis, and properties of prototype structures. Biochemistry 19:2396-2404
94. 2+ mobilization. J Biol Chem 268:11528-11533
95. Mikoshiba K, Furuichi T, Miyawaki A (1994) Structure and function of IP3 receptors. Semin Cell Biol 5:273-281
96. 2+ concentration. J Biol Chem 270:4959-4962
97. Humbert JP, Matter N, Artault JC, Köppler P, Malviya AN (1996) Inositol 1,4,5-trisphosphate receptor is located to the inner nuclear membrane vindicating regulation of nuclear calcium signaling by inositol 1,4,5-trisphosphate. Proc Natl Acad Sci 271:478-485
98. Sullivan KM, Lin DD, Wilson KL (1995) Inhibition of nuclear vesicle fusion by antibodies that block activation of inositol 1,4,5-trisphosphate receptors. Proc Nat Acad Sci 92:8611-8615
99. Dabauvalle MC, Scheer U (1991) Assembly of nuclear pore complexes in Xenopus egg extract. Biol Cell 72:25-29
100. Rothman JE, Warren G (1994) Implications of the SNARE hypothesis for intracellular membrane topology and dynamics. Curr Biol 4:220-233
101. Hallberg E, Wozniak RW, Blobel G (1993) An integral membrane protein of the pore membrane domain of the nuclear envelope contains a nucleoporin-like region. J Cell Biol 122:513-521
102. Gerace L, Ottaviano Y, Kondor-Koch C (1982) Identification of a major polypeptide of the nuclear pore complex. J Cell Biol 95:826-837
103. Wozniak RW, Bartnik E, Blobel G (1989) Primary structure analysis of an integral membrane glycoprotein of the nuclear pore. J Cell Biol 108:2083-2092
104. Greber UF, Senior A, Gerace L (1990) A major glycoprotein of the nuclear pore complex is a membrane-spanning polypeptide with a large lumenal domain and a small cytoplasmic tail. EMBO J 9:1495-1502
105. Vigers GP, Lohka MJ (1991) A distinct vesicle population targets membranes and pore complexes to the nuclear envelope in Xenopus eggs. J Cell Biol 112:545-556
106. Vigers GP, Lohka MJ (1992) Regulation of nuclear envelope precursor functions during cell division. J Cell Sci 102:273-284
107. Chaudhary N, Courvalin JC (1993) Stepwise reassembly of the nuclear envelope at the end of mitosis. J Cell Biol 122:295-306