Imaging three-dimensional single molecule orientations

Journal of Physical Chemistry B

Volumn 103, Issue 51, 1999, Pages 11237-11241

  Bartko, Andrew P ^a   Dickson, Robert M ^a  

^a GEORGIA INSTITUTE OF TECHNOLOGY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 0000040835     PISSN: 15206106     EISSN: None     Source Type: Journal    
DOI: 10.1021/jp993364q     Document Type: Article

References (31)

1. Moerner, W. E.; Kador, L. Optical detection and spectroscopy of single molecules in a solid. Phys. Rev. Lett. 1989, 62, 2535-2538.
2. Basché, T.; Kummer, S.; Bräuchle, C. Direct Spectroscopic Observation of Quantum Jumps of a Single Molecule. Nature 1995, 373, 132-134.
3. Betzig, E.; Chichester, R. J. Single molecules observed by near-field scanning optical microscopy. Science 1993, 262, 1422-1428.
4. Dickson, R. M.; Cubitt, A. B.; Tsien, R. Y.; Moerner, W. E. Blinking and switching behavior of individual green fluorescent protein molecules. Nature 1997, 388, 355-358.
5. Funatsu, T.; Harada, Y.; Tokunaga, M.; Saito, K.; Yanagida, T. Imaging of single fluorescent molecules and individual ATP turnovers by single myosin molecules in aqueous solution. Nature 1995, 374, 555-559.
6. Ha, T.; Enderle, T.; Ogletree, D. F.; Chemla, D. S.; Selvin, P. R.; Weiss, S. Probing the interaction between two single molecules: Fluorescence resonance energy transfer between a single donor and a single acceptor. Proc. Nat. Acad. Sci. USA. 1996, 93, 6264-6268.
7. Ha, T.; Glass, J.; Enderle, T.; Chemla, D. S.; Weiss, S. Hindered Rotational Diffusion and Rotational Jumps of Single Molecules. Phys. Rev. Lett. 1998, 80, 2093-2096.
8. Lu, H. P.; Xun, L.; Xie, X. S. Single-molecule enzymatic dynamics. Science 1998, 282, 1877-1882.
9. Moerner, W. E. Examining Nanoenvironments in Solids on the Scale of a Single, Isolated Molecule. Science 1994, 265, 46-53.
10. Schmidt, T.; Schutz, G. J.; Baumgartner, W.; Gruber, H. J.; Schindler, H. Imaging Of Single Molecule Diffusion. Proc. Natl. Acad. Sci. U.S.A. 1996, 93, 2926-2929.
11. Skinner, J. L.; Moerner, W. E. Structure and Dynamics in Solids as Probed by Optical Spectroscopy. J. Phys. Chem. 1996, 100, 13251-13262.
12. Weiss, S. Fluorescence spectroscopy of single biomolecules. Science 1999, 283, 1676-1683.
13. Yip, W. T.; Hu, D. H.; Yu, J.; Vanden Bout, D. A.; Barbara, P. F. Classifying the photophysical dynamics of single- and multiple-chromophoric molecules by single molecule spectroscopy. J. Phys. Chem. A 1998, 102, 7564-7575.
14. Bartko, A. P.; Dickson, R. M. Three-Dimensional Orientations of Polymer-Bound Single Molecules. J. Phys. Chem. B 1999, 103, 3053-3056.
15. Betzig, E. Proposed method for optical imaging. Opt. Lett. 1995, 20, 237-239.
16. Schuetz, G. J.; Trabesinger, W.; Schmidt, T. Direct Observation of Ligand Colocalization on Individual Receptor Molecules. Biophys. J. 1998, 74, 2223-2226.
17. Trabesinger, W.; Schütz, G. J.; Gruber, H. J.; Schindler, H.; Schmidt, T. Detection of Individual Oligonucleotide Pairing by Single Molecule Microscopy. Anal. Chem. 1999, 71, 279-283.
18. van Oijen, A. M.; Kohler, J.; Schmidt, J.; Muller, M.; Brakenhoff, G. J. Far-field fluorescence microscopy beyond the diffraction limit. J. Opt. Soc. Am. A 1999, 16, 909-915.
19. Ruiter, A. G. T.; Veerman, J. A.; Garcia-Parajo, M. F.; van Hulst, N. F. Single molecule rotational and translational diffusion observed by near-field scanning optical microscopy. J. Phys. Chem. A 1997, 101, 7318-7323.
20. Ha, T.; Enderle, T.; Chemla, D. S.; Selvin, P. R.; Weiss, S. Single molecule dynamics studied by polarization modulation. Phys. Rev. Lett. 1996, 77, 3979-3972.
21. Schuetz, G. J.; Schindler, H.; Schmidt, T. Imaging Single-Molecule Dichroism. Opt. Lett. 1997, 22, 651-653.
22. Wang, H. M.; Bardo, A. M.; Collinson, M. M.; Higgins, D. A. Microheterogeneity in Dye-Doped Silicate and Polymer Films. J. Phys. Chem. B 1998, 102, 7231-7237.
23. Xie, X. S. Single-Molecule Spectroscopy and Dynamics at Room Temperature. Acc. Chem. Res. 1996, 29, 598-606.
24. Empedocles, S. A.; Neuhauser, R.; Bawendi, M. G. Three-Dimensional orientation measurements of symmetric single chromophores using polarization microscopy. Nature 1999, 319, 126-130.
25. Macklin, J. J.; Trautman, J. K.; Harris, T. D.; Brus, L. E. Imaging and Time-Resolved Spectroscopy of Single Molecules at an Interface. Science 1996, 272, 255-258.
26. Gibson, S. F.; Lanni, F. Experimental Test of an Analytical Model of Aberration in an Oil-Immersion Objective Lens used in 3-Dimensional Light-Microscopy. J. Opt. Soc. Am. A 1991, 8, 1601-1613.
27. Axelrod, D.; Burghardt, T. P.; Thompson, N. L. Total Internal Reflection Fluorescence. Annu. Rev. Biophys. Bioeng. 1984, 13, 247-268.
28. Tokunaga, M.; Kitamura, K.; Saito, K.; Iwane, A. H.; Yanagida, T. Single molecule imaging of fluorophores and enzymatic reactions achieved by objective type total internal reflection fluorescence microscopy. Biochem. Biophys. Res. Commun. 1997, 255, 47-53.
29. Dickson, R. M.; Norris, D. J.; Moerner, W. E. Simultaneous Observation of Individual Molecules Aligned Both Parallel and Perpendicular to the Optical Axis. Phys. Rev. Lett. 1998, 81, 5322.
30. Hellen, E. H.; Axelrod, D. Fluorescence emission at dielectric and metal-film interfaces. J. Opt. Soc. Am. B 1987, 4, 337-350.
31. Sheppard, C. J. R.; Torok, P. Approximate forms for diffraction integrals in high numerical aperture focusing. Optik 1997, 105, 77-82.