Molecular characterization of an improved vector for evaluation of the tumor suppressor versus oncogene abilities of the androgen receptor

Prostate

Volumn 61, Issue 4, 2004, Pages 299-304

  Litvinov, Ivan V ^a   Antony, Lizamma ^a   Isaacs, John T ^a,b  

^a JOHNS HOPKINS UNIVERSITY   (United States)

^b 1M44 CRB ^*  (United States)

Author keywords

Androgen receptor (AR); pSG5 AR; Tumor suppressor

Indexed keywords

ANDROGEN RECEPTOR; LIGAND; PLASMID VECTOR;

3' UNTRANSLATED REGION; 5' UNTRANSLATED REGION; AR GENE; ARTICLE; BIOCHEMISTRY; CANCER CELL CULTURE; CANCER INHIBITION; CONTROLLED STUDY; DOCUMENTATION; EXPRESSION VECTOR; GENE; GENE CONSTRUCT; GENE EXPRESSION; GENE SEQUENCE; GENE VECTOR; GENETIC TRANSFECTION; GROWTH INHIBITION; HUMAN; HUMAN CELL; INTERNAL RIBOSOME ENTRY SITE; MOLECULAR CLONING; NUCLEOTIDE SEQUENCE; ONCOGENE; PAR IRES EGFP GENE; PRIORITY JOURNAL; PROSTATE CANCER; PROSTATE EPITHELIUM; PROTEIN EXPRESSION; PSG5 AR GENE; TRANSCRIPTION REGULATION; TRANSGENE; TRANSLATION REGULATION; TUMOR SUPPRESSOR GENE;

5' UNTRANSLATED REGIONS; BLOTTING, WESTERN; CELL LINE; GENE EXPRESSION; GENES, TUMOR SUPPRESSOR; GENETIC VECTORS; HUMANS; MALE; ONCOGENES; PLASMIDS; RECEPTORS, ANDROGEN; REGULATORY SEQUENCES, NUCLEIC ACID; RNA, MESSENGER; TRANSFECTION;

EID: 9444286418     PISSN: 02704137     EISSN: None     Source Type: Journal    
DOI: 10.1002/pros.20187     Document Type: Article

References (27)

1. Litvinov IV, De Marzo AM, Isaacs JT. Is the Achilles' heel for prostate cancer therapy a gain of function for androgen receptor signaling? J Clin Endocrinol Metab 2003;88(7):2972-2982.
2. Liu AY, Peehl DM. Characterization of cultured human prostatic epithelial cells by cluster designation antigen expression. Cell Tissue Res 2001;305:389-397.
3. Uzgare AR, Xu Y, Isaacs JT. In vitro culturing and characteristics of transit amplifying epithelial cells from human prostate tissue. J Cell Biochem 2004;91(1):196-205.
4. Berges RR, Vukanovic J, Epstein JI, CarMichel M, Cisek L, Johnson DE, Veltri RW, Walsh PC, Isaacs JT. Implication of the cell kinetic changes during the progression of human prostatic cancer. Clin Cancer Res 1995;1:473-480.
5. Ling MT, Chan KW, Choo CK. Androgen induces differentiation of a human papillomavirus 16 E6/E7 immortalized prostate epithelial cell line. J Endocrinol 2001;70:287-296.
6. Whitacre DC, Chauhan S, Davis T, Gordon D, Cress AE, Miesfeld RL. Androgen induction of in vitro prostate cell differentiation. Cell Growth Differ 2002;13:1-11.
7. Chen Y, Robles AI, Martinez LA, Liu F, Gimenez-Conti IB, Conti CJ. Expression of G1 cyclins, cyclin-dependent kinases, and cyclin-dependent kinase inhibitors in androgen-induced prostate proliferation in castrated rats. Cell Growth Differ 1996;7:1571-1578.
8. 1 arrest by high dose 5′- dihydrotestosterone in LNCaP human prostate cancer cells. Oncogene 2000;19:670-679.
9. Waltregny D, Leav I, Signoretti S, Soung P, Lin D, Merk F, Adams JY, Bhattacharya N, Cirenei N, Loda M. Androgen-driven prostate epithelial cell proliferation and differentiation in vivo involve the regulation of p27. Mol Endocrinol 2001;15:765-782.
10. Lu L, Schulz H, Wolf DA. The F-box protein SKP2 mediates androgen control of p27 stability in LNCaP human prostate cancer cells. BMC Cell Biol 2002;3:22.
11. De Marzo AM, Putzi MJ, Nelson WG. New concepts in the pathology of prostatic epithelial carcinogenesis. Urology 2001;57(Suppl 1):103-114.
12. Gao J, Isaacs JT. Development of an androgen receptor null model for identifying the site of initiation for androgen stimulation of proliferation and suppression of programmed (apoptotic) death of PC-82 human prostate cancer cells. Cancer Res 1998;58:3299-3306.
13. Gao J, Arnold JT, Isaacs JT. Conversion from a paracrine to an autocrine mechanism of androgen-stimulated growth during malignant transformation of prostatic epithelial cells. Cancer Res 2001;61:5038-5044.
14. Yang G, Ayala G, De Marzo A, Tian W, Frolov A, Wheeler TM, Thompson TC, Harper JW. Elevated Skp2 protein expression in human prostate cancer: Association with loss of the cyclin-dependent kinase inhibitor p27 and PTEn and with reduced recurrence-free survival. Clin Cancer Res 2002;8:3419-3426.
15. Isaacs JT, Isaacs WB. Androgen receptor outwits prostate cancer drugs. Nat Med 2004;10(1):26-27.
16. Litvinov IV, Chang C, Isaacs JT. Molecular characterization of the commonly used human androgen receptor expression vector, pSG5-AR. Prostate 2004;58(1):319-324.
17. Yeap BB, Voon DC, Vivian JP, McCulloch RK, Thomson AM, Giles KM, Czyzyk-Krzeska MF, Furneaux H, Wilce MC, Wilce JA, Leedman PF. Novel binding of HuR and poly(C)-binding protein to a conserved UC-rich motif within the 3′-untranslated region of the androgen receptor messenger RNA. J Biol Chem 2002;277:27183-27192.
18. Mizokami A, Chang C. Induction of translation of the 5′- untranslated region of human androgen receptor mRNA. J Biol Chem 1994;269:25655-25659.
19. Grossmann ME, Lindzey J, Kumar MV, Tindall DJ. The mouse androgen receptor is suppressed by the 5′-untranslated region of the gene. Mol Endocrinol 1994;8:448-455.
20. Martinez ED, Danielsen M. Loss of androgen receptor transcriptional activity at the G(1)/S transition. J Biol Chem 2002;277:29719-29729.
21. Heisler LE, Evangelou A, Lew AM, Trachtenberg J, Elsholtz HP, Brown TJ. Androgen-dependent cell cycle arrest and apoptotic death in PC-3 prostatic cell cultures expressing a full-length human androgen receptor. Mol Cell Endocrinol 1997;126:59-73.
22. Yuan S, Trachtenberg J, Mills GB, Brown Tj, Xu F, Keating A. Androgen-induced inhibition of cell proliferation in a androgen-insensitive prostate cancer cell line (PC-3) transfected with human androgen receptor complementary DNA. Cancer Res 1993;53:1304-1311.
23. Nightingale J, Chaudhary KS, Abel PD, Stubbs AP, Romanska HM, Mitchell SE, Stamp GW, Lalani N. Ligand activation of the androgen receptor downregulates e-cadherin-mediated cell adhesion and promotes apoptosis of prostatic cancer cells. Neoplasia 2003;5:347-361.
24. Davis R, Jia D, Cinar B, Sikka SC, Moparty K, Zhau HE, Chung LW, Agrawal KC, Abdel-Mageed A. Functional androgen receptor confers sensitization of androgen-independent prostate cancer cells to anticancer therapy via caspase activation. Biochem Biophys Res Commun 2003;309:937-945.
25. Uzgare AR, Isaacs JT. Enhanced redundancy in Akt and mitogen-activated protein kinase induced survival of malignant versus normal prostate epithelial cells. Cancer Res 2004:64(17): 6190-6199.
26. Yeh S, Chang C. Cloning and characterization of a specific coactivator, ARA70, for the androgen receptor in human prostate cells. Proc Natl Acad Sci USA 1996;93:5517-5521.
27. Kokontis J, Ito K, Hiipakka RA, Liao S. Expression and function of normal and LNCaP androgen receptors in androgen-insensitive human prostatic cancer cells. Receptor 1991;1(4) 271-279.