Cellular utilization determines viability and matrix distribution profiles in chondrocyte-seeded alginate constructs

Tissue Engineering

Volumn 10, Issue 9-10, 2004, Pages 1467-1479

  Heywood, Hannah K ^a   Sembi, Preetkamal K ^a   Lee, David A ^a   Bader, Dan L ^a  

^a QUEEN MARY UNIVERSITY OF LONDON   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

BIOCHEMISTRY; CELL CULTURE; MECHANICAL PROPERTIES; METABOLISM; SYNTHESIS (CHEMICAL); TISSUE;

CELLULAR UTILIZATION; EXTRACELLULAR MATRIX (ECM);

CELLS;

ALGINIC ACID;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CARTILAGE; CARTILAGE CELL; CELL CULTURE; CELL DENSITY; CELL MEMBRANE PERMEABILITY; CELL VIABILITY; CONTROLLED STUDY; EXTRACELLULAR MATRIX; NONHUMAN; PRIORITY JOURNAL; TISSUE ENGINEERING;

ADAPTATION, PHYSIOLOGICAL; ALGINATES; ANIMALS; BIOCOMPATIBLE MATERIALS; CARTILAGE, ARTICULAR; CATTLE; CELL COUNT; CELL CULTURE TECHNIQUES; CELL DIFFERENTIATION; CELL PROLIFERATION; CELL SURVIVAL; CELLS, CULTURED; CHONDROCYTES; ELASTICITY; EXTRACELLULAR MATRIX; GLUCURONIC ACID; HEXURONIC ACIDS; MATERIALS TESTING; MECHANOTRANSDUCTION, CELLULAR; PERMEABILITY; STRESS, MECHANICAL; TISSUE ENGINEERING;

EID: 9344243511     PISSN: 10763279     EISSN: None     Source Type: Journal    
DOI: 10.1089/ten.2004.10.1467     Document Type: Article

References (35)

1. Hunter, W. On the structure and disease of articular cartilage. Philos. Trans. R. Soc. Lond. B Biol. Sci. 9, 267, 1743.
2. Muir, H. The chondrocyte, architect of cartilage. Bioessays 17, 1039, 1995.
3. Newman, A.P. Articular cartilage repair. Am. J. Sports Med. 26, 309, 1998.
4. Afoke, N.Y.P., Byres, P.D., and Hutton, W.C. Contact pressures in the human hip joint. J. Bone Joint Surg. Br. 69, 536, 1987.
5. Hodge, W.A., Fijuan, R.S., Carlson, K.L., Burgess, R.G., Harris, W.H., and Mann, R.W. Contact pressures in the human hip joint measured in vivo. Proc. Natl. Acad. Sci. U.S.A. 83, 2879, 1986.
6. Ateshian, G.A., Solslowsky, L.J., and Mow, V.C. Quantitation of articular surface topography and cartilage thickness in knee joints using stereophotogrammetry. J. Biomech. 24, 761, 1991.
7. Cohen, Z.A., McCarthy, D.M., Kwak, S.D., Legrand, P., Fogarasi, F., Ciaccio, E.J., and Ateshian, G.A. Knee cartilage topography, thickness, and contact areas from MRI: In vitro calibration and in vivo measurements. Osteoarthritis Cartilage 7, 95, 1999.
8. Herberhold, C., Faber, S., Stammberger, T., Steinlechner, M., Putz, R., Englmeier, K.H., Reiser, M., and Eckstein, F. In situ measurement of articular cartilage deformation in intact femoropatellar joints under static loading. J. Biomech. 32, 1287, 1999.
9. Buschmann, M.D., Gluzband, Y.A., Grodzinsky, A.J., Kimura, J.H., and Hunziker, E.B. Chondrocytes in agarose culture synthesize a mechanically functional extracellular matrix. J. Orthop. Res. 10, 745, 1992.
10. Wong, M., Siegrist, M., and Ahsan, T. Cartilage matrix synthesis enhances the mechanical properties of alginate. Trans. Orthop. Res. Soc. 25, 0904, 2000.
11. Freed, L.E., Hollander, A.P., Martin, I., Barry, J.R., Langer, R., and Vunjak-Novakovic, G. Chondrogenesis in a cell-polymer bioreactor system. Exp. Cell Res. 240, 58, 1998.
12. LeRoux, M.A., Guilak, F., and Setton, L.A. Compressive and shear properties of alginate gel: Effects of sodium ions and alginate concentration. J. Biomed. Mater. Res. 47, 46, 1999.
13. Wong, M., Siegrist, M., Wang, X., and Hunziker, E.B. Development of mechanically stable alginate/chondrocyte constructs: Effects of glucuronic acid content and matrix synthesis. J. Orthop. Res. 19, 493, 2001.
14. Knight, M.M., van de Breevaat Bravenboer, J., Lee, D.A., van Osch, G.J.V.M., Weinans, H., and Bader, D.L. Cell and nucleus deformation in compressed chondrocyte-alginate constructs: Temporal changes and calculation of cell modulus. Biochim. Biophys. Acta 1570, 1, 2002.
15. Bonaventure, J., Kadhom, N., Cohen-Salal, L., Ng, K.H., Bourguignon, J., Lasselin, C., and Freisinger, P. Re-expression of cartilage-specific genes by dedifferentiated human articular chondrocytes cultured in alginate beads. Exp. Cell Res. 212, 97, 1994.
16. Häuselmann, H.J., Aydelotte, M.B., Schumacher, B.L., Kuettner, K.E., Gitelis, S.H., and Thonar, E.J.M.A. Synthesis and turnover of proteoglycans by human and bovine articular chondrocytes cultured in alginate beads. Matrix 12, 129, 1992.
17. Häuselmann, H.J., Fernandes, R.J., Mok, S.S., Schmid, T.M., Block, J.A., Aydelotte, M.B., Kuettner, K.E., and Thonar, E.J.M.A. Phenotypic stability of bovine articular chondrocytes after long-term culture in alginate beads. J. Cell. Sci. 107, 17, 1994.
18. Lee, D.A., Reisler, T., and Bader, D.L. Expansion of chondrocytes for tissue engineering in alginate beads enhances chondrocytic phenotype compared to conventional monolayer techniques. Acta Orthop. Scand. 74, 6, 2003.
19. Farndale, R.W., Buttle, D.J., and Barrett, A.J. Improved quantitation and discrimination of sulphated glycosaminoglycans by use of dimethylmethylene blue. Biochim. Biophys. Acta 883, 173, 1986.
20. Enobakhare, B.O., Bader, D.L., and Lee, D.A. Quantification of sulphated glycosaminoglycans in chondrocyte/alginate cultures, by use of 1,9-dimethylmethylene blue. Anal. Biochem. 243, 189, 1996.
21. Martin, I., Obradovic, B., Freed, L.E., and Vunjak-Novakovic, G. Method for the quantitative analysis of glycosaminoglycan distribution in cultured and engineered cartilage. Ann. Biomed. Eng. 27, 656, 1999.
22. Vunjak-Novakovic, G., Martin, I., Obradovic, B., Treppo, S., Grodzinsky, A.J., Langer, R., and Freed, L.E. Bioreactor cultivation conditions modulate the composition and mechanical properties of tissue-engineered cartilage. J. Orthop. Res. 17, 130, 1999.
23. Amsden, B., and Turner, N. Diffusion characteristics of calcium alginate gels. Biotechnol. Bioeng. 65, 605, 1999.
24. Smidsrød, O., and Skjak-Brask, G. Alginate as immobilisation matrix for cells. Trends Biotechnol. 8, 71, 1990.
25. Puelacher, W.C., Kim, S.W., Vacanti, J.P., Schloo, B.L., Mooney, D., and Vacanti, C.A. Tissue-engineered growth of cartilage: The effect of varying the concentration of chondrocytes seeded onto synthetic polymer matrices. Int. J. Oral Maxillofac. Surg. 23, 49, 1994.
26. Freed, L.E., Vunjak-Novakovic, G., Biron, R.J., Eagles, D.B., Lesnoy, D.C., Barlow, S.K., and Langer, R. Biodegradable polymer scaffolds for tissue engineering. Biotechnology 12, 689, 1994.
27. Gagne, T.A., Chappell-Afonso, K., Johnson, J.L., McPherson, J.M., Oldham, C.A., Tubo, R.A., Vaccaro, C., and Vasios, G.W. Enhanced proliferation and differentiation of human articular chondrocytes when seeded at low cell densities in alginate In vitro. J. Orthop. Res. 18, 882, 2000.
28. Mikkelsen, A., and Elgsaeter, A. Density distribution of calcium-induced alginate gels: A numerical study. Biopolymers 36, 17, 1995.
29. Thu, B., Gåserød, O., Paus, D., Mikkelsen, A., Skjåk-Bræk, G., Toffanin, R., Vittur, F., and Rizzo, R. Inhomogenous alginate gel spheres: An assessment of the polymer gradients by synchroton radiation-induced X-ray emission, magnetic resonance miroimaging, and mathematical modeling. Biopolymers 53, 60, 2000.
30. Enobakhare, B.O. In vitro evaluation of chondrocyte/alginate constructs for tissue engineered articular cartilage [Ph.D. thesis]. Queen Mary, University of London, London, 1998.
31. Taccheti, C., Tavella, S., Quarto, G., Robino, G., and Cancedda, R. Cell condensation in chondrogenic differentiation. Exp. Cell. Res. 200, 26, 1992.
32. Stockwell, R.A. The cell density of human articular and costal cartilage. J. Anat. 101, 753, 1967.
33. Lee, D.A., Bentley, G., and Archer, C.W. Proteoglycan depletion alone is nol sufficient to stimulate proteoglycan synthesis in cultured bovine cartilage explants. Osteoarthritis Cartilage 2, 175, 1994.
34. Verbruggen, G., Luyten, F.P., and Veys, E.M. Repair function in organ cultured human cartilage: Replacement of enzymatically removed proteoglycans during long-term organ culture. J. Rheumatol. 12, 665, 1985.
35. Handley, C.J., and Lowther, D.A. Extracellular matrix metabolism by chondrocytes. III. Modulation of proteoglycan synthesis by extracellular levels of proteoglycan in cartilage cells in culture. Biochim. Biophys. Acta 500, 132, 1977.