Conditional gene expression in renal collecting duct epithelial cells: Use of the inducible Cre-lox system

American Journal of Physiology - Renal Physiology

Volumn 286, Issue 1 55-1, 2004, Pages

  Ouvrard Pascaud, Antoine ^a   Puttini, Stefania ^a   Sainte Marie, Yannis ^a   Athman, Rafika ^b   Fontaine, Virginie ^a   Cluzeaud, Françoise ^a   Farman, Nicolette ^a   Rafestin Oblin, Marie Edith ^a   Blot Chabaud, Marcel ^a   Jaisser, Frederic ^a,c  

^a INSERM   (France)

^b INSTITUT CURIE   (France)

^c l'alimentation et l'Environnement   (France)

Author keywords

Aldosterone; Cre recombinase; Mineralocorticoid receptor; Rat cortical collecting duct 2 cells

Indexed keywords

ALDOSTERONE; CARRIER PROTEIN; CRE RECOMBINASE; GENE PRODUCT; GREEN FLUORESCENT PROTEIN; HORMONE RECEPTOR; MER RECOMBINASE; MINERALOCORTICOID RECEPTOR; PROTEIN LOXP; RECOMBINASE; REGULATOR PROTEIN; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CELL LINE; ENZYME INDUCTION; FLUID BALANCE; GENE EXPRESSION SYSTEM; HOMEOSTASIS; ION TRANSPORT; KIDNEY COLLECTING TUBULE; KIDNEY EPITHELIUM; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN STRUCTURE; RAT; STRUCTURE ACTIVITY RELATION; TRANSGENE;

EID: 9144256958     PISSN: 1931857X     EISSN: 15221466     Source Type: Journal    
DOI: 10.1152/ajprenal.00301.2002     Document Type: Article

References (34)

1. Araki K, Araki M, Miyazaki J, and Vassalli P. Site-specific recombination of a transgene in fertilized eggs by transient expression of Cre recombinase. Proc Natl Acad Sci USA 92: 160-164, 1995.
2. Bens M, Vallet V, Cluzeaud F, Pascual-Letallec L, Kahn A, Rafestin-Oblin ME, Rossier BC, and Vandewalle A. Corticosteroid-dependent sodium transport in a novel immortalized mouse collecting duct principal cell line. J Am Soc Nephrol 10: 923-934, 1999.
3. + transport in a high-resistance clone of the MDCK cell line. Pflüger Arch 432: 685-691, 1996.
4. a.Blot-Chabaud M, Laplace M, Cluzeaud F, Capurro C, Cassingena R, Vandewalle A, Farman N, and Bonvalet JP. Characteristics of a rat cortical collecting duct cell line that maintains high transepithelial resistance. Kidney Int 50: 367-376, 1996.
5. Brocard J, Feil R, Chambon P, and Metzger D. A chimeric Cre recombinase inducible by synthetic, but not by natural, ligands of the glucocorticoid-receptor. Nucleic Acids Res 26: 4086-4090, 1998.
6. Brocard J, Warot X, Wendling O, Messaddeq N, Vonesch JL, Chambon P, and Metzger D. Spatio-temporally controlled site-specific somatic mutagenesis in the mouse. Proc Natl Acad Sci USA 94: 14559-14563, 1997.
7. Bujard H. Controlling genes with tetracyclines. J Gene Med 1: 372-374, 1999.
8. De Wet JR, Wood KV, DeLuca M, Helinski DR, and Subramani S. Firefly luciferase gene: structure and expression in mammalian cells. Mol Cell Biol 7: 725-737, 1987.
9. 1-subunit dephosphorylation: study in a new aldosterone-sensitive rat cortical collecting duct cell line. J Am Soc Nephrol 12: 1805-1818, 2001.
10. Fagart J, Wurtz JM, Souque A, Hellal-Levy C, Moras D, and Rafestin-Oblin ME. Antagonism in the human mineralocorticoid receptor. EMBO J 17: 3317-3325, 1998.
11. Feil R, Brocard J, Mascrez B, LeMeur M, Metzger D, and Chambon P. Ligand-activated site-specific recombination in mice. Proc Natl Acad Sci USA 93: 10887-10890, 1996.
12. Feil R, Wagner J, Metzger D, and Chambon P. Regulation of Cre recombinase activity by mutated estrogen receptor ligand-binding domains. Biochem Biophys Res Commun 237: 752-757, 1997.
13. Fejes-Toth G, Pearce D, and Naray-Fejes-Toth A. Subcellular localization of mineralocorticoid receptors in living cells: effects of receptor agonists and antagonists. Proc Natl Acad Sci USA 95: 2973-2978, 1998.
14. Fuhrmann-Benzakein E, Garcia-Gabay I, Pepper MS, Vassalli JD, and Herrera PL. Inducible and irreversible control of gene expression using a single transgene. Nucleic Acids Res 28: E99, 2000.
15. Gouilleux F, Sola B, Couette B, and Richard-Foy H. Cooperation between structural elements in hormone-regulated transcription from the mouse mammary tumor virus promoter. Nucleic Acids Res 19: 1563-1569, 1991.
16. Houdebine LM and Attal J. Internal ribosome entry sites (IRESs): reality and use. Transgenic Res 8: 157-177, 1999.
17. T2 recombinases. Nucleic Acids Res 27: 4324-4327, 1999.
18. Jaisser F. Inducible gene expression and gene modification in transgenic mice. J Am Soc Nephrol 11: S95-S100, 2000.
19. Kellendonk C, Tronche F, Casanova E, Anlag K, Opherk C, and Schutz G. Inducible site-specific recombination in the brain. J Mol Biol 285: 175-182, 1999.
20. Kellendonk C, Tronche F, Monaghan AP, Angrand PO, Stewart F, and Schutz G. Regulation of Cre recombinase activity by the synthetic steroid RU 486. Nucleic Acids Res 24: 1404-1411, 1996.
21. Littlewood TD, Hancock DC, Danielian PS, Parker MG, and Evan GI. A modified oestrogen receptor ligand-binding domain as an improved switch for the regulation of heterologous proteins. Nucleic Acids Res 23: 1686-1690, 1995.
22. Mattioni T, Louvion JF, and Picard D. Regulation of protein activities by fusion to steroid binding domains. Methods Cell Biol 43: 335-352, 1994.
23. Metzger D, Clifford J, Chiba H, and Chambon P. Conditional site-specific recombination in mammalian cells using a ligand-dependent chimeric Cre recombinase. Proc Natl Acad Sci USA 92: 6991-6995, 1995.
24. Metzger D and Feil R. Engineering the mouse genome by site-specific recombination. Curr Opin Biotechnol 10: 470-476, 1999.
25. Nishi M, Ogawa H, Ito T, Matsuda KI, and Kawata M. Dynamic changes in subcellular localization of mineralocorticoid receptor in living cells: compared with glucocorticoid receptor using dual-color labeling with green fluorescent protein spectral variants. Mol. Endocrinol 15: 1077-1092, 2001.
26. Odermatt A, Arnold P, and Frey FJ. The intracellular localization of the mineralocorticoid receptor is regulated by 11β-hydroxysteroid dehydrogenase type 2. J Biol Chem 276: 28484-28492, 2001.
27. Puttini S, Beggah AT, Ouvrard-Pascaud A, Legris C, Blot-Chabaud M, Farman N, and Jaisser F. Tetracycline-inducible gene expression in cultured rat renal CD cells and in intact CD from transgenic mice. Am J Physiol Renal Physiol 281: F1164-F1172, 2001.
28. Sambrook J, Fritsch EF, and Maniatis T. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory, 1989.
29. Sauer B and Henderson N. Cre-stimulated recombination at loxP-containing DNA sequences placed into the mammalian genome. Nucleic Acids Res 17: 147-161, 1989.
30. Schwenk F, Kuhn R, Angrand PO, Rajewsky K, and Stewart AF. Temporally and spatially regulated somatic mutagenesis in mice. Nucleic Acids Res 26: 1427-1432, 1998.
31. Vandewalle A. Immortalized kidney cells derived from transgenic mice harboring L-type pyruvate kinase and vimentin promoters. Exp Nephrol 7: 386-393, 1999.
32. Wunderlich FT, Wildner H, Rajewsky K, and Edenhofer F. New variants of inducible Cre recombinase: a novel mutant of Cre-PR fusion protein exhibits enhanced sensitivity and an expanded range of inducibility. Nucleic Acids Res 29: E47, 2001.
33. Zhang Y, Riesterer C, Ayrall AM, Sablitzky F, Littlewood TD, and Reth M. Inducible site-directed recombination in mouse embryonic stem cells. Nucleic Acids Res 24: 543-548, 1996.
34. Zhang Y, Wienands J, Zurn C, and Reth M. Induction of the antigen receptor expression on B lymphocytes results in rapid competence for signaling of SLP-65 and Syk. EMBO J 17: 7304-7310, 1998.