Process development for a recombinant Chinese Hamster Ovary (CHO) cell line utilizing a metal induced and amplified metallothionein expression system

Biotechnology and Bioengineering

Volumn 88, Issue 4, 2004, Pages 437-450

  Huang, Edwin P ^a   Marquis, Christopher P ^a   Gray, Peter P ^a  

^a UNIVERSITY OF NEW SOUTH WALES   (Australia)

Author keywords

Acoustic; Chinese hamster ovary cells; Fed batch; Human growth hormone; Metallothionein; Perfusion

Indexed keywords

BIOREACTORS; CADMIUM; FERMENTATION; HORMONES; PROTEINS; SODIUM COMPOUNDS;

CHINESE HAMSTER OVARY (CHO); HUMAN GROWTH HORMONE (HGH); METALLOTHIONEIN (MT); SPECIFIC FEEDING RATES (SFR);

CELLS;

BUTYRIC ACID; HUMAN GROWTH HORMONE; METAL; METALLOTHIONEIN;

AMPLIFIED METALLOTHIONEIN EXPRESSION SYSTEM; ANIMAL CELL; ARTICLE; BIOREACTOR; CELL LINE; CELL SUSPENSION; CELL VIABILITY; CHO CELL; FED BATCH REACTOR; FERMENTATION; GENE EXPRESSION SYSTEM; GENETIC RECOMBINATION; NONHUMAN; PROCESS DEVELOPMENT; PROMOTER REGION; VOLUMETRY;

ANIMALS; BIOREACTORS; CADMIUM; CELL CULTURE TECHNIQUES; CELL PROLIFERATION; CELL SURVIVAL; CHO CELLS; CRICETINAE; CRICETULUS; DOSE-RESPONSE RELATIONSHIP, DRUG; GENE EXPRESSION REGULATION; HUMAN GROWTH HORMONE; METALLOTHIONEIN; METALS; PILOT PROJECTS; PROTEIN ENGINEERING; RECOMBINANT PROTEINS; ZINC;

CRICETINAE; CRICETULUS GRISEUS;

EID: 8844280068     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/bit.20194     Document Type: Article

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