Expression, purification and structural characterization of the scaffoldin hydrophilic X-module from the cellulosome of Clostridium thermocellum

Protein Expression and Purification

Volumn 38, Issue 2, 2004, Pages 258-263

  Adams, Jarrett J ^a   Jang, Christopher J ^a   Spencer, Holly L ^a   Elliott, Marc ^b   Smith, Steven P ^a  

^a QUEEN S UNIVERSITY   (Canada)

^b UNIVERSITY OF TORONTO   (Canada)

Author keywords

Cellulose degradation; Cellulosome; Clostridium thermocellum; X module

Indexed keywords

BACTERIAL PROTEIN; CELLULASE; CELLULOSOME, CLOSTRIDIUM; CIPA PROTEIN, CLOSTRIDIUM; HYBRID PROTEIN; MEMBRANE PROTEIN; MULTIENZYME COMPLEX; PROTEIN SUBUNIT;

AMINO ACID SEQUENCE; ARTICLE; CHEMISTRY; CLOSTRIDIUM; COMPARATIVE STUDY; ENZYMOLOGY; GENE EXPRESSION REGULATION; GENETICS; ISOLATION AND PURIFICATION; MOLECULAR CLONING; MOLECULAR GENETICS; SEQUENCE HOMOLOGY;

AMINO ACID SEQUENCE; BACTERIAL PROTEINS; CELLULASE; CLONING, MOLECULAR; CLOSTRIDIUM; GENE EXPRESSION REGULATION, ENZYMOLOGIC; MEMBRANE PROTEINS; MOLECULAR SEQUENCE DATA; MULTIENZYME COMPLEXES; PROTEIN SUBUNITS; RECOMBINANT FUSION PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID;

BACTERIA (MICROORGANISMS); CLOSTRIDIUM; CLOSTRIDIUM THERMOCELLUM; ESCHERICHIA COLI; FUNGI; VECTORS;

EID: 8844256267     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2004.08.018     Document Type: Article

References (27)

1. R.H. Doi, and A. Kosugi Cellulosomes: plant-cell-wall-degrading enzyme complexes Nat. Rev. Microbiol. 2 2004 541 551
2. W.H. Schwarz The cellulosome and cellulose degradation by anaerobic bacteria Appl. Microbiol. Biotechnol. 56 2001 634 649
3. P. Beguin, and P.M. Alzari The cellulosome of Clostridium thermocellum Biochem. Soc. Trans. 26 1998 178 185
4. E.A. Bayer, L.J. Shimon, Y. Shoham, and R. Lamed Cellulosomes-structure and ultrastructure J. Struct. Biol. 124 1998 221 234
5. E.A. Bayer, E. Morag, and R. Lamed The cellulosome-a treasure-trove for biotechnology Trends. Biotechnol. 12 1994 379 386
6. E.A. Bayer, R. Kenig, and R. Lamed Adherence of Clostridium thermocellum to cellulose J. Bacteriol. 156 1983 818 827
7. R. Lamed, R. Kenig, E. Setter, and E.A. Bayer The cellulosome: a discrete cell surface organelle of Clostridium thermocellum which exhibits separate antigenic, cellulose-binding and various cellulolytic activities Biotechnol. Bioeng. Symp. 13 1983 163 181
8. E.A. Bayer, H. Chanzy, R. Lamed, and Y. Shoham Cellulose, cellulases and cellulosomes Curr. Opin. Struct. Biol. 8 1998 548 557
9. S.J. Charnock, D.N. Bolam, J.P. Turkenburg, H.J. Gilbert, L.M. Ferreira, G.J. Davies, and C.M. Fontes The X6 "thermostabilizing" domains of xylanases are carbohydrate-binding modules: structure and biochemistry of the Clostridium thermocellum X6b domain Biochemistry 39 2000 5013 5021
10. Q. Xu, M. Morrison, K.E. Nelson, E.A. Bayer, N. Atamna, and R. Lamed A novel family of carbohydrate-binding modules identified with Ruminococcus albus proteins FEBS Lett. 566 2004 11 16
11. I.A. Kataeva, R.D. Seidel 3rd, A. Shah, L.T. West, X.L. Li, and L.G. Ljungdahl The fibronectin type 3-like repeat from the Clostridium thermocellum cellobiohydrolase CbhA promotes hydrolysis of cellulose by modifying its surface Appl. Environ. Microbiol. 68 2002 4292 4300
12. I.A. Kataeva, V.N. Uversky, and L.G. Ljungdahl Calcium and domain interactions contribute to the thermostability of domains of the multimodular cellobiohydrolase, CbhA, a subunit of the Clostridium thermocellum cellulosome Biochem. J. 372 2003 151 161
13. U.T. Gerngross, M.P. Romaniec, T. Kobayashi, N.S. Huskisson, and A.L. Demain Sequencing of a Clostridium thermocellum gene (cipA) encoding the cellulosomal SL-protein reveals an unusual degree of internal homology Mol. Microbiol. 8 1993 325 334
14. S.K. Choi, and L.G. Ljungdahl Structural role of calcium for the organization of the cellulosome of Clostridium thermocellum Biochemistry 35 1996 4906 4910
15. B. Lytle, C. Myers, K. Kruus, and J.H. Wu Interactions of the CelS binding ligand with various receptor domains of the Clostridium thermocellum cellulosomal scaffolding protein, CipA J. Bacteriol. 178 1996 1200 1203
16. S. Yaron, E. Morag, E.A. Bayer, R. Lamed, and Y. Shoham Expression, purification and subunit-binding properties of cohesins 2 and 3 of the Clostridium thermocellum cellulosome FEBS Lett. 360 1995 121 124
17. E. Morag, A. Lapidot, D. Govorko, R. Lamed, M. Wilchek, E.A. Bayer, and Y. Shoham Expression, purification, and characterization of the cellulose-binding domain of the scaffoldin subunit from the cellulosome of Clostridium thermocellum Appl. Environ. Microbiol. 61 1995 1980 1986
18. P. Beguin, and M. Lemaire The cellulosome: an exocellular, multiprotein complex specialized in cellulose degradation Crit. Rev. Biochem. Mol. Biol. 31 1996 201 236
19. S.P. Smith, P. Beguin, P.M. Alzari, and K. Gehring 1H, 13C, 15N NMR sequence-specific resonance assignment of a Clostridium thermocellum type II cohesin module J. Biomol. NMR 23 2002 73 74
20. M. Lemaire, I. Miras, P. Gounon, and P. Beguin Identification of a region responsible for binding to the cell wall within the S-layer protein of Clostridium thermocellum Microbiology 144 1998 211 217
21. Q. Xu, W. Gao, S.Y. Ding, R. Kenig, Y. Shoham, E.A. Bayer, and R. Lamed The cellulosome system of Acetivibrio cellulolyticus includes a novel type of adaptor protein and a cell surface anchoring protein J. Bacteriol. 185 2003 4548 4557
22. S.Y. Ding, E.A. Bayer, D. Steiner, Y. Shoham, and R. Lamed A novel cellulosomal scaffoldin from Acetivibrio cellulolyticus that contains a family 9 glycosyl hydrolase J. Bacteriol. 181 1999 6720 6729
23. G. Bohm, R. Muhr, and R. Jaenicke Quantitative analysis of protein far UV circular dichroism spectra by neural networks Protein Eng. 5 1992 191 195
24. L.E. Kay, P. Keiffer, and T. Saarinen Pure absorption gradient enhanced heteronuclear single quantum correlation spectroscopy with improved sensitivity J. Am. Chem. Soc. 114 1992 10663 10665
25. F. Delaglio, S. Grzesiek, G.W. Vuister, G. Zhu, J. Pfeifer, and A. Bax NMRPipe: a multidimensional spectral processing system based on UNIX pipes J. Biomol. NMR 6 1995 277 293
26. B.A. Johnson, and R.A. Blevins NMRVIEW: a computer program for the visualization and analysis of NMR data J. Biomol. NMR 4 1994 603 614
27. A. Mosbah, A. Belaich, O. Bornet, J.P. Belaich, B. Henrissat, and H. Darbon Solution structure of the module X2 1 of unknown function of the cellulosomal scaffolding protein CipC of Clostridium cellulolyticum J. Mol. Biol. 304 2000 201 217