The cellulosome system of Acetivibrio cellulolyticus includes a novel type of adaptor protein and a cell surface anchoring protein

Journal of Bacteriology

Volumn 185, Issue 15, 2003, Pages 4548-4557

  Xu, Qi ^a   Gao, Wenchen ^a   Ding, Shi You ^a,b   Kenig, Rina ^a   Shoham, Yuval ^c   Bayer, Edward A ^b   Lamed, Raphael ^a  

^a TEL AVIV UNIVERSITY   (Israel)

^b WEIZMANN INSTITUTE OF SCIENCE   (Israel)

^c TECHNION ISRAEL INSTITUTE OF TECHNOLOGY   (Israel)

Author keywords

[No Author keywords available]

Indexed keywords

ADAPTOR PROTEIN; BACTERIAL PROTEIN; CELL SURFACE ANCHORING PROTEIN; CELL SURFACE PROTEIN; CELLULOSE; CELLULOSOME; COHESIN; COHESIN 1; COHESIN 2; COHESIN 3; COHESIN 4; DOCKERIN; GLYCOSIDASE; PEPTIDE FRAGMENT; PROLYLTHREONYLALANYLSERYL PEPTIDE FRAGMENT; SCAA PROTEIN; SCAC PROTEIN; SCAFFOLDIN; SIGNAL PEPTIDE; THREONINE; UNCLASSIFIED DRUG;

ACETIVIBRIO; AFFINITY BLOTTING; AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; BACTERIAL GENE; BLOTTING; CARBOHYDRATE METABOLISM; CELL ORGANELLE; CELLULOSE BINDING DOMAIN; CIPV GENE; DNA SEQUENCE; ENZYME SUBUNIT; GENE CLUSTER; MOLECULAR PROBE; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; PHYLOGENY; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN FAMILY; PROTEIN FUNCTION; PROTEIN INTERACTION; PROTEIN STRUCTURE; SCAB GENE; SCAC GENE; SCAFFOLDING GENE; VIBRIO;

AMINO ACID SEQUENCE; BACTERIA, ANAEROBIC; BACTERIAL PROTEINS; CARRIER PROTEINS; CELL MEMBRANE; CELLULASE; CELLULOSE; GLYCOSIDE HYDROLASES; MEMBRANE PROTEINS; MOLECULAR SEQUENCE DATA; MULTIENZYME COMPLEXES; PHYLOGENY; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS, DNA;

ACETIVIBRIO CELLULOLYTICUS; BACTERIA (MICROORGANISMS); NEGIBACTERIA; PROKARYOTA; VIBRIO;

EID: 0038443570     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.185.15.4548-4557.2003     Document Type: Article

References (60)

1. Bairoch, A., and R. Apweiler. 2000. The SWISS-PROT protein sequence database and its supplement TrEMBL in 2000. Nucleic Acids Res. 28:45-48.
2. Bayer, E. A., H. Chanzy, R. Lamed, and Y. Shoham. 1998. Cellulose, cellulases and cellulosomes. Curr. Opin. Struct. Biol. 8:548-557.
3. Bayer, E. A., R. Kenig, and R. Lamed. 1983. Adherence of Clostridium thermocellum to cellulose. J. Bacteriol. 156:818-827.
4. Bayer, E. A., E. Morag, and R. Lamed. 1994. The cellulosome - a treasuretrove for biotechnology. Trends Biotechnol. 12:378-386.
5. Bayer, E. A., Y. Shoham, and R. Lamed. September 2000, revision date. Cellulose-decomposing prokaryotes and their enzyme systems. In M. Dworkin, S. Falkow, E. Rosenberg, K.-H. Schleifer, and E. Stackebrandt (ed.), The prokaryotes: an evolving electronic resource for the microbiological community, 3rd ed., release 3.7. [Online.] http://link.springer.de/link/service/books/10125/index.htm. Springer-Verlag, New York, N.Y.
6. Bayer, E. A., and M. Wilchek. 1990. Protein biotinylation. Methods Enzymol. 184:138-160.
7. Béguin, P., S. Chauvaux, G. Guglielmi, M. Matuschek, E. Leibovitz, M.-K. Chaveroche, I. Miras, P. Alzari, and P. Gounon. 1999. The Clostridium thermocellum cellulosome: organization and mode of attachment to the cell, p. 437-443. In K. Ohmiya, K. Hayashi, K. Sakka, Y. Kobayashi, S. Karita, and T. Kimura (ed.), Genetics, biochemistry and ecology of cellulose degradation. Uni Publishers Co., Tokyo, Japan.
8. Béguin, P., and M. Lemaire. 1996. The cellulosome: an exocellular, multiprotein complex specialized in cellulose degradation. Crit. Rev. Biochem. Mol. Biol. 31:201-236.
9. Belaich, J.-P., A. Belaich, H.-P. Fierobe, L. Gal, C. Gaudin, S. Pagès, C. Reverbel-Leroy, and C. Tardif. 1999. The cellulolytic system of Clostridium cellulolyticum, p. 479-487. In K. Ohmiya, K. Hayashi, K. Sakka, Y. Kobayashi, S. Karita, and T. Kimura (ed.), Genetics, biochemistry and ecology of cellulose degradation. Uni Publishers Co., Tokyo, Japan.
10. Bourne, Y., and B. Henrissat. 2001. Glycoside hydrolases and glycosyltransferases: families and functional modules. Curr. Opin. Struct. Biol. 11:593-600.
11. Chauvaux, S., P. Béguin, J.-P. Aubert, K. M. Bhat, L. A. Gow, T. M. Wood, and A. Bairoch. 1990. Calcium-binding affinity and calcium-enhanced activity of Clostridium thermocellum endoglucanase D. Biochem. J. 265:261-265.
12. Chauvaux, S., M. Matuschek, and P. Béguin. 1999. Distinct affinity of binding sites for S-layer homologous domains in Clostridium thermocellum and Bacillus anthracis cell envelopes. J. Bacteriol. 181:2455-2458.
13. Colvin, J. R., L. C. Sowden, G. B. Patel, and A. W. Khan. 1982. The ultrastructure of Acetivibrio cellulolyticus, a recently isolated cellulolytic anaerobe. Curr. Microbiol. 7:13-17.
14. Coutinho, P. M., and B. Henrissat. 1999. Carbohydrate-active enzymes: an integrated database approach, p. 3-12. In H. J. Gilbert, G. J. Davies, B. Henrissat, and B. Svensson (ed.), Recent advances in carbohydrate bioengineering. The Royal Society of Chemistry, Cambridge, United Kingdom.
15. Ding, S.-Y., E. A. Bayer, D. Steiner, Y. Shoham, and R. Lamed. 1999. A novel cellulosomal scaffoldin from Acetivibrio cellulolyticus that contains a family 9 glycosyl hydrolase. J. Bacteriol. 181:6720-6729.
16. Ding, S.-Y., E. A. Bayer, D. Steiner, Y. Shoham, and R. Lamed. 2000. A scaffoldin of the Bacteroides cellulosolvens cellulosome that contains 11 type II cohesins. J. Bacteriol. 182:4915-4925.
17. Ding, S.-Y., M. T. Rincon, R. Lamed, J. C. Martin, S. I. McCrae, V. Aurilia, Y. Shoham, E. A. Bayer, and H. J. Flint. 2001. Cellulosomal scaffoldin-like proteins from Ruminococcus flavefaciens. J. Bacteriol. 183:1945-1953.
18. Doi, R. H., and Y. Tamura. 2001. The Clostridium cellulovorans cellulosome: an enzyme complex with plant cell wall degrading activity. Chem. Rec. 1:24-32.
19. Engelhardt, H., and J. Peters. 1998. Structural research on surface layers: a focus on stability, surface layer homology domains, and surface layer-cell wall interactions. J. Struct. Biol. 124:276-302.
20. Felix, C. R., and L. G. Ljungdahl. 1993. The cellulosome - the exocellular organelle of Clostridium. Annu. Rev. Microbiol. 47:791-819.
21. Fierobe, H.-P., A. Mechaly, C. Tardif, A. Belaich, R. Lamed, Y. Shoham, J.-P. Belaich, and E. A. Bayer. 2002. Designer nanosomes: selective engineering of dockerin-containing enzymes into chimeric scaffoldins to form defined nanoreactors, p. 113-123. In T. T. Teeri, B. Svensson, H. J. Gilbert, and T. Feizi (ed.), Carbohydrate bioengineering: interdisciplinary approaches. The Royal Society of Chemistry, Cambridge, United Kingdom.
22. Fujino, T., P. Béguin, and J.-P. Aubert. 1993. Organization of a Clostridium thermocellum gene cluster encoding the cellulosomal scaffolding protein CipA and a protein possibly involved in attachment of the cellulosome to the cell surface. J. Bacteriol. 175:1891-1899.
23. L-protein reveals an unusual degree of internal homology. Mol. Microbiol. 8:325-334.
24. Gerwig, G., P. de Waard, J. P. Kamerling, J. F. G. Vliegenthart, E. Morgenstern, R. Lamed, and E. A. Bayer. 1989. Novel O-linked carbohydrate chains in the cellulase complex (cellulosome) of Clostridium thermocellum. J. Biol. Chem. 264:1027-1035.
25. Gerwig, G., J. P. Kamerling, J. F. G. Vliegenthart, E. Morag (Morgenstern), R. Lamed, and E. A. Bayer. 1992. Novel oligosaccharide constituents of the cellulase complex of Bacteroides cellulosolvens. Eur. J. Biochem. 205:799-808.
26. Gerwig, G., J. P. Kamerling, J. F. G. Vliegenthart, E. Morag, R. Lamed, and E. A. Bayer. 1991. Primary structure of O-linked carbohydrate chains in the cellulosome of different Clostridium thermocellum strains. Eur. J. Biochem. 196:115-122.
27. Gerwig, G., J. P. Kamerling, J. F. G. Vliegenthart, E. Morag, R. Lamed, and E. A. Bayer. 1993. The nature of the carbohydrate-peptide linkage region in glycoproteins from the cellulosomes of Clostridium thermocellum and Bacteroides cellulosolvens. J. Biol. Chem. 268:26956-26960.
28. Herzberg, O., and M. N. James. 1988. Refined crystal structure of troponin C from turkey skeletal muscle at 2.0 A resolution. J. Mol. Biol. 203:761-779.
29. Israel, D. I. 1993. A PCR-based method for high stringency screening of DNA libraries. Nucleic Acids Res. 21:2627-2631.
30. Kakiuchi, M., A. Isui, K. Suzuki, T. Fujino, E. Fujino, T. Kimura, S. Karita, K. Sakka, and K. Ohmiya. 1998. Cloning and DNA sequencing of the genes encoding Clostridium josui scaffolding protein CipA and cellulase CelD and identification of their gene products as major components of the cellulosome. J. Bacteriol. 180:4303-4308.
31. Khan, A. W. 1980. Cellulolytic enzyme system of Acetivibrio cellulolyticus, a newly isolated anaerobe. J. Gen. Microbiol. 121:499-502.
32. Lamed, R., and E. A. Bayer. 1988. The cellulosome of Clostridium thermocellum. Adv. Appl. Microbiol. 33:1-46.
33. Lamed, R., R. Kenig, E. Setter, and E. A. Bayer. 1985. Major characteristics of the cellulolytic system of Clostridium thermocellum coincide with those of the purified cellulosome. Enzyme Microb. Technol. 7:37-41.
34. Lamed, R., J. Naimark, E. Morgenstern, and E. A. Bayer. 1987. Scanning electron microscopic delineation of bacterial surface topology using cationized ferritin. J. Microbiol. Methods 7:233-240.
35. Lamed, R., J. Naimark, E. Morgenstern, and E. A. Bayer. 1987. Specialized cell surface structures in cellulolytic bacteria. J. Bacteriol. 169:3792-3800.
36. Lamed, R., E. Setter, and E. A. Bayer. 1983. Characterization of a cellulose-binding, cellulase-containing complex in Clostridium thermocellum. J. Bacteriol. 156:828-836.
37. Lamed, R., E. Setter, R. Kenig, and E. A. Bayer. 1983. The cellulosome - a discrete cell surface organelle of Clostridium thermocellum which exhibits separate antigenic, cellulose-binding and various cellulolytic activities. Biotechnol. Bioeng. Symp. 13:163-181.
38. Leibovitz, E., and P. Béguin. 1996. A new type of cohesin domain that specifically binds the dockerin domain of the Clostridium thermocellum cellulosome-integrating protein CipA. J. Bacteriol. 178:3077-3084.
39. Leibovitz, E., H. Ohayon, P. Gounon, and P. Béguin. 1997. Characterization and subcellular localization of the Clostridium thermocellum scaffoldin dockerin binding protein SdbA. J. Bacteriol. 179:2519-2523.
40. Lemaire, M., I. Miras, P. Gounon, and P. Béguin. 1998. Identification of a region responsible for binding to the cell wall within the S-layer protein of Clostridium thermocellum. Microbiology 144:211-217.
41. Lemaire, M., H. Ohayon, P. Gounon, T. Fujino, and P. Béguin. 1995. OlpB, a new outer layer protein of Clostridium thermocellum, and binding of its S-layer-like domains to components of the cell envelope. J. Bacteriol. 177:2451-2459.
42. Lupas, A., H. Engelhardt, J. Peters, U. Santarius, S. Volker, and W. Baumeister. 1994. Domain structure of the Acetogenium kivui surface layer revealed by electron crystallography and sequence analysis. J. Bacteriol. 176:1224-1233.
43. Lytle, B., B. F. Volkman, W. M. Westler, and J. H. D. Wu. 2000. Secondary structure and calcium-induced folding of the Clostridium thermocellum dockerin domain determined by NMR spectroscopy. Arch. Biochem. Biophys. 379:237-244.
44. Mechaly, A., H.-P. Fierobe, A. Belaich, J.-P. Belaich, R. Lamed, Y. Shoham, and E. A. Bayer. 2001. Cohesin-dockerin interaction in cellulosome assembly: a single hydroxyl group of a dockerin domain distinguishes between non-recognition and high-affinity recognition. J. Biol. Chem. 276:9883-9888.
45. Mechaly, A., A. Teplitsky, V. Belakhov, T. Baasov, G. Shoham, and Y. Shoham. 2000. Overproduction and characterization of seleno-methionine xylanase T-6. J. Biotechnol. 78:83-86.
46. Mechaly, A., S. Yaron, R. Lamed, H.-P. Fierobe, A. Belaich, J.-P. Belaich, Y. Shoham, and E. A. Bayer. 2000. Cohesin-dockerin recognition in cellulosome assembly: experiment versus hypothesis. Proteins 39:170-177.
47. Mesnage, S., E. Tosi-Couture, M. Mock, and A. Fouet. 1999. The S-layer homology domain as a means for anchoring heterologous proteins on the cell surface of Bacillus anthracis. J. Appl. Microbiol. 87:256-260.
48. Murray, M. G., and W. F. Thompson. 1980. Rapid isolation of high-molecular-weight plant DNA. Nucleic Acids Res. 8:4321-4325.
49. Nielsen, H., S. Brunak, and G. von Heijne. 1999. Machine learning approaches for the prediction of signal peptides and other protein sorting signals. Protein Eng. 12:3-9.
50. Pagès, S., A. Belaich, J.-P. Belaich, E. Morag, R. Lamed, Y. Shoham, and E. A. Bayer. 1997. Species-specificity of the cohesin-dockerin interaction between Clostridium thermocellum and Clostridium cellulolyticum: prediction of specificity determinants of the dockerin domain. Proteins 29:517-527.
51. Pagès, S., A. Belaich, H.-P. Fierobe, C. Tardif, C. Gaudin, and J.-P. Belaich. 1999. Sequence analysis of scaffolding protein CipC and ORFXp, a new cohesin-containing protein in Clostridium cellulolyticum: comparison of various cohesin domains and subcellular localization of ORFXp. J. Bacteriol. 181:1801-1810.
52. Rincon, M. T., S.-Y. Ding, S. I. McCrae, J. C. Martin, V. Aurilia, R. Lamed, Y. Shoham, E. A. Bayer, and H. J. Flint. 2003. Novel organization and divergent dockerin specificities in the cellulosome system of Ruminococcus flavefaciens. J. Bacteriol. 185:703-713.
53. Rost, B. 1996. PHD: predicting one-dimensional protein structure by profile based neural networks. Methods Enzymol. 266:525-539.
54. Rost, B., and C. Sander. 1994. Combining evolutionary information and neural networks to predict protein secondary structure. Proteins 19:55-72.
55. Rost, B., and C. Sander. 1993. Prediction of protein secondary structure at better than 70% accuracy. J. Mol. Biol. 232:584-599.
56. Sabathe, F., A. Belaich, and P. Soucaille. 2002. Characterization of the cellulolytic complex (cellulosome) of Clostridium acetobutylicum. FEMS Microbiol. Lett. 217:15-22.
57. Saddler, J. N., and A. W. Khan. 1980. Cellulase production by Acetivibrio cellulolyticus. Can. J. Microbiol. 26:760-765.
58. Schwarz, W. H. 2001. The cellulosome and cellulose degradation by anaerobic bacteria. Appl. Microbiol. Biotechnol. 56:634-649.
59. Shoham, Y., R. Lamed, and E. A. Bayer. 1999. The cellulosome concept as an efficient microbial strategy for the degradation of insoluble polysaccharides. Trends Microbiol. 7:275-281.
60. Shoseyov, O., M. Takagi, M. A. Goldstein, and R. H. Doi. 1992. Primary sequence analysis of Clostridium cellulovorans cellulose binding protein A. Proc. Natl. Acad. Sci. USA 89:3483-3487.