Isogenic auxotrophic mutant strains in the Aspergillus fumigatus genome reference strain AF293

Archives of Microbiology

Volumn 182, Issue 5, 2004, Pages 346-353

  Xue, Tao ^a   Nguyen, Cuong K ^a   Romans, Angela ^a   Kontoyiannis, Dimitrios P ^b   May, Gregory S ^a  

^a UNIVERSITY OF TEXAS   (United States)

^b UNIVERSITY OF TEXAS   (United States)

Author keywords

Auxotroph; Homologous recombination; Transformation

Indexed keywords

ARGININE; LYSINE;

ARTICLE; ASPERGILLUS FUMIGATUS; AUXOTROPHIC MUTANT; CONTROLLED STUDY; DIPLOIDY; FUNGAL STRAIN; GENE DELETION; GENE FUNCTION; GENE LOCUS; GENETIC ANALYSIS; GENETIC STABILITY; GENETIC TRANSFORMATION; GENOME; MOLECULAR GENETICS; NONHUMAN; NUCLEOTIDE SEQUENCE; PLASMID; PRIORITY JOURNAL;

ARGININE; ASPERGILLUS FUMIGATUS; BACTERIAL PROTEINS; CULTURE MEDIA; DNA, FUNGAL; GENE DELETION; GENOME, FUNGAL; HUMANS; LYSINE; MUTATION; PLASMIDS; RECOMBINATION, GENETIC; TRANSFORMATION, GENETIC;

ASPERGILLUS; ASPERGILLUS FUMIGATUS; FUNGI;

EID: 8644272378     PISSN: 03028933     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00203-004-0707-z     Document Type: Article

References (35)

1. Brown JS, Aufauvre-Brown A, Brown J, Jennings JM, Arst H, Holden DW (2000) Signature-tagged and directed mutagenesis identify PABA synthetase as essential for Aspergillus fumigatus pathogenicity. Mol Microbiol 36:1371-1380
2. Calera JA, Paris S, Monod M, Hamilton AJ, Debeaupuis JP, Diaquin M, Lopez-Medrano R, Leal F, Latge JP (1997) Cloning and disruption of the antigenic catalase gene of Aspergillus fumigatus. Infect Immun 65:4718-4724
3. Carrol AM, Sweigard JA, Valent B (1994) Improved vectors for selecting resistance to hygromycin. Fungal Genet Newsl 41:22
4. Cove DJ (1966) The induction and repression of nitrate reductase in the fungus Aspergillus nidulans. Biochim Biophys Acta 113:51-56
5. De Lucas JR, Dominguez AI, Higuero Y, Martinez O, Romero B, Mendoza A, Garcia-Bustos JF, Laborda F (2001) Development of a homologous transformation system for the opportunistic human pathogen Aspergillus fumigatus based on the sC gene encoding ATP sulfurylase. Arch Microbiol 176:106-113
6. d'Enfert C (1996) Selection of multiple disruption events in Aspergillus fumigatus using the orotidine-5′-decarboxylase gene, pyrG, as a unique transformation marker. Curr Genet 30:76-82
7. d'Enfert C, Diaquin M, Delit A, Wuscher N, Debeaupuis JP, Huerre M, Latge JP (1996) Attenuated virulence of uridine-uracil auxotrophs of Aspergillus fumigatus. Infect Immun 64:4401-4405
8. Fincham JR (1989) Transformation in fungi. Microbiol Rev 53:148-170
9. Firon A, Beauvais A, Latge JP, Couve E, Grosjean-Cournoyer MC, d'Enfert C (2002) Characterization of essential genes by parasexual genetics in the human fungal pathogen Aspergillus fumigatus: impact of genomic rearrangements associated with electroporation of DNA. Genetics 161:1077-1087
10. Firon A, Villalba F, Beffa R, d'Enfert C (2003) Identification of essential genes in the human fungal pathogen Aspergillus fumigatus by transposon mutagenesis. Eukaryot Cell 2:247-255
11. Fonzi WA, Irwin MY (1993) Isogenic strain construction and gene mapping in Candida albicans. Genetics 134:717-728
12. Grallert B, Nurse P, Patterson TE (1993) A study of integrative transformation in Schizosaccharomyces pombe. Mol Gen Genet 238:26-32
13. Grimm C, Kohli J, Murray J, Maundrell K (1988) Genetic engineering of Schizosaccharomyces pombe: a system for gene disruption and replacement using the ura4 gene as a selectable marker. Mol Gen Genet 215:81-86
14. Hensel M, Arst HN, Aufauvre-Brown A, Holden DW (1998) The role of the Aspergillus fumigatus are A gene in invasive pulmonary aspergillosis. Mol Gen Genet 258:553-557
15. Kwon-Chung KJ, Goldman WE, Klein B, Szaniszlo PJ (1998) Fate of transforming DNA in pathogenic fungi. Med Mycol 36:38-44
16. Langfelder K, Gattung S, Brakhage AA (2002) A novel method used to delete a new Aspergillus fumigatus ABC transporter-encoding gene. Curr Genet 41:268-274
17. Ma H, Kunes S, Schatz PJ, Botstein D (1987) Plasmid construction by homologous recombination in yeast. Gene 58:201-216
18. Magrini V, Goldman WE (2001) Molecular mycology: a genetic toolbox for Histoplasma capsulatum. Trends Microbiol 9:541-546
19. May GS, Gambino J, Weatherbee JA, Morris NR (1985) Identification and functional analysis of β-tubulin genes by site specific integrative transformation in Aspergillus nidulans. J Cell Biol 101:712-719
20. McDade HC, Cox GM (2001) A new dominant selectable marker for use in Crvptococcus neoformans. Med Mycol 39:151-154
21. Mellado E, Specht CA, Robbins PW, Holden DW (1996a) Cloning and characterization of chsD, a chitin synthase-like gene of Aspergillus fumigatus. FEMS Microbiol Lett 143:69-76
22. Mellado E, Aufauvre-Brown A, Gow NA, Holden DW (1996b) The Aspergillus fumigatus chsC and chsG genes encode class III chitin synthases with different functions. Mol Microbiol 20:667-679
23. Miller BL, Miller KY, Timberlake WE (1985) Direct and indirect gene replacements in Aspergillus nidulans. Mol Cell Biol 5:1714-1721
24. Nelson RT, Pryor BA, Lodge JK (2003) Sequence length required for homologous recombination in Cryptococcus neoformans. Fungal Genet Biol 38:1-9
25. Osherov N, May G (2000) Conidial germination in Aspergillus nidulans requires RAS signaling and protein synthesis. Genetics 155:647-656
26. Osherov N, Mathew J, May GS (2000) Polarity-defective mutants of Aspergillus nidulans. Fungal Genet Biol 31:181-188
27. Osherov N, Kontoyiannis DP, Romans A, May GS (2001) Resistance to itraconazole in Aspergillus nidulans and Aspergillus fumigatus is conferred by extra copies of the A. nidulans P-450 14alpha- demethylase gene, pdmA. J Antimicrob Chemother 48:75-81
28. Prado F, Aguilera A (1994) New in vivo cloning methods by homologous recombination in yeast. Curr Genet 25:180-183
29. Reichard U, Hung CY, Thomas PW, Cole GT (2000) Disruption of the gene which encodes a serodiagnostic antigen and chitinase of the human fungal pathogen Coccidioides immitis. Infect Immun 68:5830-5838
30. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory, Cold Spring Harbor
31. Staab JF, Sundstrom P (2003) URA3 as a selectable marker for disruption and virulence assessment of Candida albicans genes. Trends Microbiol 11:69-73
32. Tang CM, Smith JM, Arst HN, Holden DW (1994) Virulence studies of Aspergillus nidulans mutants requiring lysine or p-aminobenzoic acid in invasive pulmonary aspergillosis. Infect Immun 62:5255-5260
33. Upshall A (1986) Genetic and molecular characterization of argB+ transformants of Aspergillus nidulans. Curr Genet 10:593-599
34. Weidner G, d'Enfert C, Koch A, Mol PC, Brakhage AA (1998) Development of a homologous transformation system for the human pathogenic fungus Aspergillus fumigatus based on the pyrG gene encoding orotidine 5′-monophosphate decarboxylase. Curr Genet 33:378-385
35. Wright AP, Maundrell K, Shall S (1986) Transformation of Schizosaccharomyces pombe by non-homologous, unstable integration of plasmids in the genome. Curr Genet 10:503-508