The Aspergillus fumigatus chsC and chsG genes encode Class III chitin synthases with different functions

Molecular Microbiology

Volumn 20, Issue 3, 1996, Pages 667-679

  Mellado, Emilia ^a   Aufauvre Brown, Agnès ^a   Gow, Neil A R ^b   Holden, David W ^a  

^a HAMMERSMITH HOSPITAL   (United Kingdom)

^b UNIVERSITY OF ABERDEEN   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

CHITIN SYNTHASE;

ANIMAL EXPERIMENT; ARTICLE; ASPERGILLUS FUMIGATUS; CONTROLLED STUDY; DNA SEQUENCE; ENZYME ACTIVITY; FUNGUS MUTANT; MORTALITY; MOUSE; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL;

AMINO ACID SEQUENCE; ANIMALS; ASPERGILLUS FUMIGATUS; BASE SEQUENCE; CHITIN; CHITIN SYNTHASE; DNA, FUNGAL; FUNGAL PROTEINS; GENE DELETION; MICE; MOLECULAR SEQUENCE DATA; VIRULENCE;

EID: 0029925168     PISSN: 0950382X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2958.1996.5571084.x     Document Type: Article

References (41)

1. Bowen, A.R., Chen-Wu, J.L., Momany, M., Young, R., Szaniszlo, P.J., and Robbins, P.W. (1992) Classification of fungal chitin synthases. Proc Natl Acad Sci USA 89: 519-523.
2. Bulawa, C.E. (1992) CSD2, CSD3, and CSD4, genes required for chitin synthesis in Saccharomyces cerevisiae: the CSD2 gene product is related to chitin synthases and to developmentally regulated proteins in Rhizobium species and Xenopus laevis. Mol Cell Biol 12: 1764-1776.
3. Bulawa, C.E. (1993) Genetics and molecular biology of chitin synthesis in fungi. Annu Rev Microbiol 47: 505-534.
4. Bulawa, C.E., Slater, M., Cabib, E., Au-Young, J., Sburlati, A., Adair, W.L., and Robbins, P.W. (1986) The S. cerevisiae structural gene for chitin synthase is not required for chitin synthesis in vivo. Cell 46: 213-225.
5. Bull, A.T. (1970) Chemical composition of wild type and mutant Aspergillus nidulans cell walls. The nature of polysaccharide and melanin constituents. J Gen Microbiol 63: 75-94.
6. Cabib, E. (1987) The synthesis and degradation of the chitin. Adv Enzymol Relat Areas Mol Biol 59: 59-101.
7. Choi, W.J., Sburlati, A., and Cabib, E. (1994) Chitin synthase 3 from yeast has zymogenic properties that depend on both the CAL1 and the CAL3 genes. Proc Natl Acad Sci USA 91: 4727-4730.
8. Cove, D.J. (1966) The induction and repression of nitrate reductase in the fungus Aspergillus nidulans. Biochem Biophys Acta 113: 51-56.
9. Cullen, D., Leong, S.A., Wilson, L.J., and Henner, D.J. (1987) Transformation of Aspergillus nidulans with the hygromycin resistance gene hph. Gene 57: 21-26.
10. Devereux, J., Haeberli, P., and Smithies, O. (1984) A comprehensive set of sequence analysis programs for the VAX. Nucl Acids Res 12: 387-395.
11. Din, A.B., and Yarden, O. (1994) The Neurospora crassa chs-2 gene encodes a non-essential chitin synthase. Microbiology 140: 2189-2197.
12. Drocourt, D., Calmels, T., Reynes, J.P., Baron, M., and Tiraby, G. (1990) Cassettes of the Streptoalloteichus hindustanus ble gene for transformation of lower and higher eukaryotes to phleomycin resistance. Nucl Acids Res 18: 4009.
13. + channels in the hyphal tip plasma membrane of the oomycete Saprolegnia ferax. J Cell Sci 101: 721-730.
14. Gooday, G.W. (1977) Biosynthesis of the fungal wall. Mechanisms and implications. J Gen Microbiol 99: 1-11.
15. Gow, N.A.R., Miller, P.F.P., and Gooday, G.W. (1992) Life at the apex: growth of the apical tip. J Chem Tech Biotech 56: 217-219.
16. Gray, D.I., and Morris, B.M. (1992) A low cost video analysis system for the BBC master computer. Binary 4: 58-61.
17. Gurr, S.J., Unkles, S.E., and Kinghorn, J.R. (1987) The structure and organization of nuclear genes of filamentous fungi. In Gene Structure in Eukaryotic Microbes. Kinghorn, J.R. (ed.). Oxford, USA: IRL Press, pp. 93-139.
18. Holden, D.W., Kronstad, J.W., and Leong, S.A. (1989) Mutation in a heat regulated hsp70 gene of Ustilago maydis. EMBO J 8: 1927-1934.
19. Kozak, M. (1981) Compilation and analysis of sequences upstream from the translational start site in eukaryotic mRNAs. Nucl Acids Res 12: 857-872.
20. Kyte, J., and Doolittle, R.F. (1982) A simple method for displaying the hydropathic character of a protein. J Mol Biol 157: 105-132.
21. Mellado, E., Aufauvre-Brown, A., Specht, C.A., Robbins, P.W., and Holden, D.W. (1995) A multigene family related to chitin synthase genes of yeast in the opportunistic pathogen Aspergillus fumigatus. Mol Gen Genet 246: 353-359.
22. Mishra, N.C., and Tatum, E.L. (1972) Effect of L-sorbose on polysaccharide synthetases of Neurospora crassa. Proc Natl Acad Sci USA 69: 313-317.
23. Motoyama, T., Kojima, N., Horiuchi, H., Ohta, A., and Takagi, M. (1994a) Isolation of a chitin synthase gene (chsC) of Aspergillus nidulans. Biosci Biotech Biochem 58: 2254-2257.
24. Motoyama, T., Sudoh, M., Horiuchi, H., Ohta, A., and Takagi, M. (1994b) Isolation and characterization of two chitin synthase genes of Rhizopus oligosporus. Biosci Biotech Biochem 58: 1685-1693.
25. Nagahashi, S., Sudoh, M., Ono, N., Sawada, R., Yamaguchi, E., Uchida, Y., Mio, T., Takagi, M., Arisawa, M., and Yamada-Okabe, H. (1995) Characterization of chitin synthase 2 of Saccharomyces cerevisiae. Implication of two highly conserved domains as possible catalytic sites. J Biol Chem 270: 13961-13967.
26. Pearson, W.R. (1990) Rapid and sensitive sequence comparison with FASTP and FASTA. Meth Enzymol 183: 63-98.
27. Pless, D.D., and Lennarz, W.J. (1977) Enzymatic conversion of proteins to glycoproteins. Proc Natl Acad Sci USA 74: 134-138.
28. Proudfoot, N.J., and Brownlee, G.G. (1976) 3′ Non-coding region sequences in eukaryotic messenger RNA. Nature 263: 211-214.
29. Sambrook, J., Fritsch, E.F., and Maniatis, T. (1989) Molecular cloning: A Laboratory Manual. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press.
30. Sanger, F., Nicklen, S., and Coulson, A.R. (1977) DNA sequencing with chain terminating inhibitors. Proc Natl Acad Sci USA 74: 5463-5467.
31. Sburlati, A., and Cabib, E. (1986) Chitin synthase 2, a presumptive participant in septum formation in Saccharomyces cerevisiae. J Biol Chem 261: 15147-15152.
32. Shaw, J.A., Mol, P.C., Bowers, B., Silverman, S.J., Valdivieso, M.H., Durán, A., and Cabib, E. (1991) The function of chitin synthases 2 and 3 in the Saccharomyces cerevisiae cell cycle. J Cell Biol 114: 111-123.
33. Silverman, S.J. (1989) Similar and different domains of chitin synthases 1 and 2 of Saccharomyces cerevisiae: two isoenzymes with different functions. Yeast 5: 459-467.
34. Smith, J.M., Tang, C.M., Van Noorden, S., and Holden, D.W. (1994) Virulence of Aspergillus fumigatus double mutants lacking restrictocin and an alkaline protease in a low-dose model of invasive pulmonary aspergillosis. Infect Immun 62: 5247-5254.
35. Tang, C.M., Cohen, J., and Holden, D.W. (1992) An Aspergillus fumigatus alkaline protease mutant constructed by gene disruption is deficient in extracellular elastase activity. Mol Microbiol 6: 1663-1671.
36. Trinci, A.P.J. (1974) A study of the kinetics of hyphal extension and branch initiation of fungal mycelia. J Gen Microbiol 81: 225-236.
37. Trinci, A.P.J., and Collinge, A. (1973) Influence of L-sorbose on growth and morphology of Neurospora crassa. J Gen Microbiol 78: 179-192.
38. Valdivieso, M.H., Mol, P.C., Shaw, J.A., Cabib, E., and Durán, A. (1991) CAL1, a gene required for activity of chitin synthase 3 in Saccharomyces cerevisiae. J Cell Biol 114: 101-109.
39. Wheat, R.W. (1966) Analysis of hexosamines in bacterial polysaccharides by chromatographic procedures. Meth Enzymol 8: 60-78.
40. Yanai, K., Kojima, N., Takaya, N., Horiuchi, H., Ohta, A., and Takagi, M. (1994) Isolation and characterization of two chitin synthase genes from Aspergillus nidulans. Biosci Biotech Biochem 58: 1828-1835.
41. Yarden, O., and Yanofsky, C. (1991) Chitin synthase 1 plays a role in cell wall biogenesis in Neurospora crassa. Genes Dev 5: 2420-2430.