Evaluation of cardosin A as a proteolytic probe in the presence of organic solvents

Journal of Molecular Catalysis B: Enzymatic

Volumn 31, Issue 4-6, 2004, Pages 137-141

  Sarmento, A Cristina ^a   Oliveira, Cláudia S ^a   Pires, Euclides M ^b   Halling, Peter J ^c   Barros, Marlene T ^a,d  

^a UNIVERSITY OF AVEIRO   (Portugal)

^b UNIVERSITY OF COIMBRA   (Portugal)

^c UNIVERSITY OF STRATHCLYDE   (United Kingdom)

^d Portuguese Catholic University Viseu   (Portugal)

Author keywords

Aspartic proteinases; Enzyme catalysis; Hydrolysis intermediates; Solvent effects; Specificity

Indexed keywords

CARDOSIN A; ETHYL ACETATE; PEPTIDES;

BIOLOGICAL MEMBRANES; ENZYMES; HYDROLYSIS; HYDROPHOBICITY; INSULIN; PROTEINS;

ORGANIC SOLVENTS;

ACETIC ACID ETHYL ESTER; CARDOSIN A; HEXANE; INSULIN; MEMBRANE PROTEIN; ORGANIC SOLVENT; PROTEINASE; UNCLASSIFIED DRUG;

ARTICLE; BETA CHAIN; CHEMICAL BOND; CHEMICAL MODIFICATION; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME SPECIFICITY; ENZYME SUBSTRATE; HYDROLYSIS; HYDROPHOBICITY; MOLECULAR PROBE; OXIDATION; PROTEIN DEGRADATION; PROTEIN STRUCTURE; REACTION TIME; SOLVENT EFFECT;

EID: 8644265982     PISSN: 13811177     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.molcatb.2004.09.001     Document Type: Article

References (26)

1. W.K. Russell, Z.Y. Park, and D.H. Russell Anal. Chem. 73 2001 2682
2. L. Nikolova, K. Soman, J.C. Nichols, D.S. Daniel, B.F. Dickey, and S. Hoffenberg Biochem. J. 336 1998 461
3. V. Redeker, S. Lachkar, S. Siavoshian, E. Charbaut, J. Rossier, A. Sobel, and P.A. Curmi J. Biol. Chem. 275 2000 6841
4. S.J. Hubbard Biochim. Biophys. Acta 1382 1998 191
5. R.V. Ulijn, A.E.M. Janssen, B.D. Moore, P.H. Halling, S.M. Kelly, and N.C. Price Chem. Biochem. 3 2002 1112
6. G. Pencreac'h, and J.C. Baratti Enzyme Microbial Technol. 28 2001 473
7. A.K. Gladilin, and A.V. Levashov Biochemistry (Moscow) 63 1998 345
8. A.E. Janssen, A.M. Vaidya, and P.J. Halling Enzyme Microbial Technol. 18 1996 340
9. G. Carrea, and S. Riva Angew. Chem. Int. Ed. 39 2000 2226
10. C.R. Wescott, and A.M. Klibanov Biochim. Biophys. Acta 1206 1994 1
11. A. Zaks, and A.M. Klibanov J. Biol. Chem. 7 1988 3194
12. P. Veríssimo, C. Faro, A.J.G. Moir, Y. Lin, J. Tang, and E. Pires Eur. J. Biochem. 235 1996 762
13. C. Frazão, I. Bento, J. Costa, C.M. Soares, P. Veríssimo, C. Faro, E. Pires, and M.A. Carrondo J. Biol. Chem. 274 1999 27694
14. M. Ramalho-Santos, P. Veríssimo, L. Cortes, B. Samyn, J. Beeumen, E. Pires, and C. Faro Eur. J. Biochem. 255 1998 133
15. M. Ramalho-Santos, J. Pissarra, P. Veríssimo, S. Pereira, R. Salema, E. Pires, and C. Faro Planta 203 1997 204
16. A.C. Sarmento, L. Silvestre, M. Barros, and E. Pires J. Mol. Catal. B: Enzymatic 5 1998 327
17. A.C. Sarmento, M. Barros, and E. Pires Stability and stabilization of biocatalists Progress in Biotechnology 1998 Elsevier New York pp. 731
18. A.C. Sarmento, C.S. Oliveira, E. Pires, F. Amado, and M. Barros J. Mol. Catal. B: Enzymatic 28 2004 33
19. U.K. Laemmli Nature 227 1970 685
20. R.L. Heinrikson, and S.C. Meredith Anal. Biochem. 163 1984 157
21. C.G. Knight Methods Enzymol. 88 1966 85
22. B.M. Dunn, and S.-H. Hung Biochim. Biophys. Acta 1477 2000 231
23. C.J. Faro, A.J.G. Moir, and E. Pires Biotechnol. Lett. 14 1992 841
24. K. Xu, K. Griebenow, and A.M. Klibanov Biotechnol. Bioeng. 56 1997 485
25. J.S. Fruton Q. Rev. Biol. 77 2002 127
26. B.M. Dunn Chem. Rev. 102 2002 4431