Asymptomatic HIV infection is characterized by rapid turnover of HIV RNA in plasma and lymph nodes but not of latently infected lymph-node CD4+ T cells

AIDS

Volumn 11, Issue 9, 1997, Pages 1103-1110

  Stellbrink, Hans Jürgen ^a   Van Lunzen, Jan ^a   Hufert, Frank T ^b   Fröschle, Günter ^a   Wolf Vorbeck, Guido ^c   Zöllner, Bernhard ^a   Albrecht, Helmut ^a   Greten, Heiner ^a   Racz, Paul ^d   Tenner Racz, Klara ^d  

^a UNIVERSITY MEDICAL CENTER HAMBURG EPPENDORF   (Germany)

^b UNIVERSITY OF FREIBURG   (Germany)

^c UNIVERSITY OF FREIBURG   (Germany)

^d BERNHARD NOCHT INSTITUTE FOR TROPICAL MEDICINE   (Germany)

Author keywords

Antiviral therapy; Asymptomatic infection; Combination therapy; Kinetics; Latency; Latently infected cells; Lymph node tissue; Plasma viraemia; Virus production

Indexed keywords

ANTIVIRUS AGENT; CD4 ANTIGEN; SAQUINAVIR; VIRUS RNA; ZALCITABINE; ZIDOVUDINE;

ADULT; ARTICLE; CLINICAL ARTICLE; FEMALE; GERMINAL CENTER; HUMAN; HUMAN CELL; HUMAN IMMUNODEFICIENCY VIRUS INFECTION; HUMAN TISSUE; LYMPH NODE; LYMPH NODE BIOPSY; MALE; PLASMA; PRIORITY JOURNAL; T LYMPHOCYTE; VIREMIA; VIRUS REPLICATION;

ADULT; ANTI-HIV AGENTS; CD4 LYMPHOCYTE COUNT; CD4-POSITIVE T-LYMPHOCYTES; DNA, VIRAL; FEMALE; HIV INFECTIONS; HIV-1; HUMANS; IN SITU HYBRIDIZATION; KINETICS; LYMPH NODES; MALE; MIDDLE AGED; PROVIRUSES; RNA, MESSENGER; RNA, VIRAL; VIREMIA; VIRUS REPLICATION;

EID: 8544278899     PISSN: 02699370     EISSN: None     Source Type: Journal    
DOI: 10.1097/00002030-199709000-00004     Document Type: Article

References (23)

1. Nuovo GJ, Becker J, Burk MW, et al.: In situ detection of PCR-amplified HIV-1 nucleic acids in lymph nodes and peripheral blood in patients with asymptomatic HIV-1 infection and advanced-stage AIDS. J Acquir Immune Defic Syndr 1994, 7:916-923.
2. Van Kerckhoven I, Fransen KR, Peeters M, et al.: Quantification of human immunodeficiency virus in plasma by RNA PCR, viral culture, and p24 antigen detection. J Clin Microbiol 1994, 32:1669-1673.
3. Piatak M, Saag M, Yang L, et al.: High levels of HIV-1 in plasma during all stages of infection determined by competitive PCR. Science 1993, 259:1749-1754.
4. Ho DD, Neumann AU, Perelson AS, et al.: Rapid turnover of plasma virions and CD4 lymphocytes in HIV-1 infection. Nature 1995, 373:123-126.
5. Wei X, Ghosh SK, Taylor ME, et al.: Viral dynamics in human immunodeficiency virus type 1 infection. Nature 1995, 373:117-122.
6. Stellbrink HJ, Zöllner B, Fenner T, et al.: Rapid plasma virus and CD4+ T-cell turnover in HIV-1 infection: evidence for an only transient interruption by treatment. AIDS 1996, 10:849-857.
7. Carpenter CCJ, Fischl MA, Hammer SM, et al.: Antiretroviral therapy for HIV infection in 1996. Recommendations of an international panel. JAMA 1996, 276:146-154.
8. Racz P, Tenner-Racz K, Kahl C, et al.: Spectrum of morphologic changes of lymph nodes from patients with AIDS or AIDS-related complexes. Prog Allergy 1986, 37:81-181.
9. Biberfeld P, Chayt K, Marselle L, et al.: HTLV-III expression in infected lymph nodes and relevance to pathogenesis of lymphadenopathy. Am J Pathol 1986, 125:436-442.
10. Embretson J, Zupancic M, Ribas J, et al.: Massive covert infection of helper T lymphocytes and macrophages by HIV during the incubation period of AIDS. Nature 1993, 362:359-362.
11. Pantaleo G, Graziosi C, Demarest JF, et al.: HIV infection is active and progressive in lymphoid tissue during the clinically latent stage of disease. Nature 1993, 362:355-358.
12. Armstrong J, Horne R: Follicular dendritic cells and virus like particles in AIDS-related lymphadenopathy. Lancet 1984, 2:370-372.
13. Baroni C, Pezzella F, Mirolo M, et al.: Immunohistochemical demonstration of p24 HTLV-III major core protein in different cell types within lymph nodes from patients with lymphadenopathy syndrome (LAS). Histopathology 1986, 10:5-13.
14. Fox C, Tenner-Racz K, Racz P, et al.: Lymphoid germinal centers are reservoirs of human immunodeficiency virus type 1 RNA. J Infect Dis 1991, 164:1051-1057.
15. Pantaleo G, Graziosi C, Butini I, et al.: Lymphoid organs function as major reservoirs for human immunodeficiency virus. Proc Natl Acad Sci U S A 1991, 88:9838-9842.
16. Spiegel H, Herbst H, Niedobitek G, et al.: Follicular dendritic cells are a major reservoir for human immunodeficiency virus type 1 in lymph nodes facilitating infection of CD4 T-helper cells. Am J Pathol 1992, 140:15-22.
17. Mulder J, McKinney N, Kwok S: A rapid and simple sample preparation method dramatically increases sensitivity of HIV-I plasma RNA assay. XI International Conference on AIDS. Vancouver, July 1996 [abstract ThB914].
18. Bertram S, Hufert FT, Neumann-Haefelen D, et al.: Detection of DNA in single cells using an automated cell deposition unit and PCR. Biotechniques 1995, 19:616-620.
19. Mellors JW, Kingsley LA, Rinaldo Jr CR, et al.: Quantitation of HIV-1 RNA in plasma predicts outcome after seroconversion. Ann Intern Med 1995, 122:573-579.
20. Tenner-Racz K, Racz P, Bofill M, et al.: HTLV-III/LAV viral antigens in lymph nodes from homosexual men with persistent generalized lymphadenopathy and AIDS. Am J Pathol 1986, 123:9-15.
21. Cohen OJ, Pantaleo G, Holodniy M, et al.: Antiretroviral monotherapy in early stage human immunodeficiency virus disease has no detectable effect on virus load in peripheral blood and lymph nodes. J Infect Dis 1996, 173:844-856.
22. Schmitz J, van Lunzen J, Tenner-Racz K, et al.: Follicular dendritic cells retain HIV-1 particles on their plasma membrane, but are not productively infected in asymptomatic patients with follicular hyperplasia. J Immunol 1994, 133:1352-1359.
23. Lafeuillade A, Poggi C, Profizi N, et al.: Human immunodeficiency virus type 1 kinetics in lymph nodes compared with plasma. J Infect Dis 1996, 174:404-407.