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Volumn 14, Issue 18, 2004, Pages 4633-4637

In vitro anti-parasitic activity of Cyclosporin A analogs on Trypanosoma cruzi

Author keywords

Cyclophilin; Cyclosporin A; Peptidyl prolyl cis trans isomerase; Trypanocidal

Indexed keywords

CYCLOSPORIN A; SDZ 211810;

EID: 8544277247     PISSN: 0960894X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bmcl.2004.07.003     Document Type: Article
Times cited : (31)

References (28)
  • 13
    • 8544264849 scopus 로고    scopus 로고
    • note
    • 5 parasites/mL, were cultured in biphasic medium containing 1.5% nutrient agar with the addition of 0.2% rabbit defibrinated blood and Brain Heart Infusion medium (Difco Michigan, USA) at 28°C. Drug concentrations ranging from 0 to 200 μM were added. The diluent, ethanol, remained below 0.125%. Parasite concentration was microscopically determined in samples obtained at 48, 72, 96, and 144 h of incubation using a Neubauer chamber
  • 16
    • 8544271137 scopus 로고    scopus 로고
    • note
    • Effect of CsA and CsA derivatives on trypomastigote lysis: the assay was performed in 96-well microplate with 50.000 bloodstream trypomastigotes per well incubated with different drug concentrations ranging from 0 to 400 μM. Cultures were incubated for 24 h at 4°C at a final volume of 0.1 mL. Drug lysis was evaluated by counting remaining parasites in an haemocytometer. The final diluent concentration ethanol remained below 0.125%, including in drug nontreated control cultures
  • 17
    • 8544280231 scopus 로고    scopus 로고
    • note
    • 2 and then, thoroughly washed with serum-free medium and incubated with complete medium without drugs for 36 h. Infected and drug nontreated cell cultures were used as controls with 0.3% ethanol. Cell cultures were set up in triplicate for each drug concentration. Covers with cells were removed from the culture plates after 36 h of treatment, gently rinsed with phosphate buffer saline, air dried, fixed in ethanol, and stained with Giemsa. Statistical analysis: experiments were performed in duplicate or in triplicate, as indicated, in two to four independent experiments. The dose response curves were obtained by linear regression analysis using computer programs. Statistical analysis of the in vitro parasite experiments and enzyme kinetics was performed using Student's t test. p < 0.05 was regarded as statistically significant
  • 25
    • 8544230897 scopus 로고    scopus 로고
    • note
    • 21,22 The effect of CsA and derivatives on PPIase activity was analyzed by incubating 20 nM of purified TcCyP19 during 5 min at 5°C with different concentrations of drugs ranging from 0 to 1 μM


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.