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Volumn 274, Issue 5295, 1996, Pages 2063-2065

Molecular basis of gene-for-gene specificity in bacterial speck disease of tomato

Author keywords

[No Author keywords available]

Indexed keywords

AGRICULTURE; ARTICLE; BACTERIAL GENE; DISEASE RESISTANCE; NONHUMAN; PRIORITY JOURNAL; PSEUDOMONAS SYRINGAE; TOMATO;

EID: 0030475465     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.274.5295.2063     Document Type: Article
Times cited : (438)

References (22)
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    • note
    • 9 cfu/ml. The full-length and delted AvrPto constructs were cloned into the pBIN19 derivative pMD1. Positive clones were introduced into Agrobacterium via electroporation.
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    • note
    • Mutations were introduced to the Pto gene sequence by recombinant polymerase chain reaction with the use of cloned Pto and Fen templates (D. T. L., J.P.R. and J.H.C., unpublished data). Recombinant PCR products were cloned into the yeast two-hybrid vector pAS2-1 (Clontech) and sequenced before testing. Wild-type and mutant avrPto genes were cloned into a pACT2 (Clontech).
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    • note
    • We thank D. O. Lavelle, J. Gardner, and J. Luke for DNA sequencing and G. Oldroyd, M. B. Mudgett, S. Barker, V. Szabo, and L. Doyle for comments on the manuscript. Supported by NSF Cooperative Agreement BIR-8920216 to CEPRAP and by CEPRAP corporate associates Calgene, Inc., Ciba Geigy Biotechnology Corporation, Sandoz Seeds, and Zeneca Seeds.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.