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Volumn 9, Issue 1, 2020, Pages 1259-1268

ddPCR: a more accurate tool for SARS-CoV-2 detection in low viral load specimens

Author keywords

clinical detection; droplet digital PCR; false negative; RT PCR; SARS CoV 2

Indexed keywords

COMPLEMENTARY DNA; VIRUS DNA; VIRUS RNA;

EID: 85086051263     PISSN: None     EISSN: 22221751     Source Type: Journal    
DOI: 10.1080/22221751.2020.1772678     Document Type: Article
Times cited : (332)

References (20)
  • 1
    • 85081626097 scopus 로고    scopus 로고
    • A new coronavirus associated with human respiratory disease in China
    • 579:265–269
    • Wu F, Zhao S, Yu B, et al. A new coronavirus associated with human respiratory disease in China. Nature. 2020;579:265–269.
    • (2020) Nature
    • Wu, F.1    Zhao, S.2    Yu, B.3
  • 2
    • 85078975424 scopus 로고    scopus 로고
    • RNA based mNGS approach identifies a novel human coronavirus from two individual pneumonia cases in 2019 Wuhan outbreak
    • Chen L, Liu W, Zhang Q, et al. RNA based mNGS approach identifies a novel human coronavirus from two individual pneumonia cases in 2019 Wuhan outbreak. Emerg Microbes Infect. 2020; 9: 313–319. doi: 10.1080/22221751.2020.1725399
    • (2020) Emerg Microbes Infect , vol.9 , pp. 313-319
    • Chen, L.1    Liu, W.2    Zhang, Q.3
  • 3
    • 85086786705 scopus 로고    scopus 로고
    • Laboratory testing for 2019 novel coronavirus (2019-nCoV) suspected human cases [Internet]. 2020 [cited 2020 Feb 4]. p. 1–7. Available from
    • World Health Organization. Laboratory testing for 2019 novel coronavirus (2019-nCoV) in suspected human cases [Internet]. 2020 [cited 2020 Feb 4]. p. 1–7. Available from: https://www.who.int/publications-detail/laboratory-testing-for-2019-novel-coronavirus-in-suspected-human-cases-20200117.
  • 4
    • 85086780265 scopus 로고    scopus 로고
    • Diagis and treatment of pneumonitis with a new type of coronavirus infection (trial version 5) [Internet]. 2020 [cited 2020 Feb 6]. Available from
    • General Office of the National Health and Health Commission O of the SA of TCM. Diagnosis and treatment of pneumonitis with a new type of coronavirus infection (trial version 5) [Internet]. 2020 [cited 2020 Feb 6]. Available from: http://bgs.satcm.gov.cn/zhengcewenjian/2020-02-06/12848.html.
  • 5
    • 85095078843 scopus 로고    scopus 로고
    • Covert coronavirus infections could be seeding new outbreaks [Internet]. Nat. news. 2020 [cited 2020 Mar 20]. Available from
    • Qiu J., Covert coronavirus infections could be seeding new outbreaks [Internet]. Nat. news. 2020 [cited 2020 Mar 20]. Available from: https://doi.org/10.1038/d41586-020-00822-x.
    • Qiu, J.1
  • 6
    • 85086786897 scopus 로고    scopus 로고
    • Characteristics of and important lessons from the coronavirus disease 2019 (COVID-19) outbreak China: summary of a report of 72 314 cases from the chinese center for disease control and prevention. Jama [Internet]. 2020;2019:25–28. Available from
    • Wu Z, McGoogan JM., Characteristics of and important lessons from the coronavirus disease 2019 (COVID-19) outbreak in China: summary of a report of 72 314 cases from the chinese center for disease control and prevention. Jama [Internet]. 2020;2019:25–28. Available from: http://www.ncbi.nlm.nih.gov/pubmed/32091533.
    • Wu, Z.1    McGoogan, J.M.2
  • 7
    • 0035988627 scopus 로고    scopus 로고
    • Quantification using real-time PCR technology: applications and limitations
    • Klein D., Quantification using real-time PCR technology: applications and limitations. Trends Mol Med. 2002; 8: 257–260. doi: 10.1016/S1471-4914(02)02355-9
    • (2002) Trends Mol Med , vol.8 , pp. 257-260
    • Klein, D.1
  • 8
    • 85086789186 scopus 로고    scopus 로고
    • Comparison and analysis of the detection performance of six new coronavirus nucleic acid detection reagents
    • Guo Y, Wang K, Zhang Y, et al. Comparison and analysis of the detection performance of six new coronavirus nucleic acid detection reagents. Chongqing Med. 2020; 14: 1671–8348.
    • (2020) Chongqing Med , vol.14 , pp. 1671-8348
    • Guo, Y.1    Wang, K.2    Zhang, Y.3
  • 9
    • 85019578498 scopus 로고    scopus 로고
    • Applications of digital PCR for clinical microbiology
    • Kuypers J, Jerome KR., Applications of digital PCR for clinical microbiology. J Clin Microbiol. 2017; 55: 1621–1628. doi: 10.1128/JCM.00211-17
    • (2017) J Clin Microbiol , vol.55 , pp. 1621-1628
    • Kuypers, J.1    Jerome, K.R.2
  • 11
    • 0347762809 scopus 로고    scopus 로고
    • Principle and applications of digital PCR
    • Pohl G, Shih I-M., Principle and applications of digital PCR. Expert Rev Mol Diagn. 2004; 4: 41–47. doi: 10.1586/14737159.4.1.41
    • (2004) Expert Rev Mol Diagn , vol.4 , pp. 41-47
    • Pohl, G.1    Shih, I.-M.2
  • 12
    • 84884478678 scopus 로고    scopus 로고
    • Evaluation of digital PCR for absolute RNA quantification
    • Sanders R, Mason DJ, Foy CA, et al. Evaluation of digital PCR for absolute RNA quantification. PLoS One. 2013; 8: e75296. doi: 10.1371/journal.pone.0075296
    • (2013) PLoS One , vol.8
    • Sanders, R.1    Mason, D.J.2    Foy, C.A.3
  • 13
    • 85086784701 scopus 로고    scopus 로고
    • Digital PCR provides sensitive and absolute calibration for high throughput sequencing
    • White RA, Blainey PC, Fan HC, et al. Digital PCR provides sensitive and absolute calibration for high throughput sequencing. BMC Genomics. 2009; 10: 110–116. doi: 10.1186/1471-2164-10-110
    • (2009) BMC Genomics , vol.10 , pp. 110-116
    • White, R.A.1    Blainey, P.C.2    Fan, H.C.3
  • 14
    • 81255175501 scopus 로고    scopus 로고
    • High-throughput droplet digital PCR system for absolute quantitation of DNA copy number
    • Hindson BJ, Ness KD, Masquelier DA, et al. High-throughput droplet digital PCR system for absolute quantitation of DNA copy number. Anal Chem. 2011; 83: 8604–8610. doi: 10.1021/ac202028g
    • (2011) Anal Chem , vol.83 , pp. 8604-8610
    • Hindson, B.J.1    Ness, K.D.2    Masquelier, D.A.3
  • 15
    • 84884902737 scopus 로고    scopus 로고
    • Absolute quantification by droplet digital PCR versus analog real-time PCR
    • Hindson CM, Chevillet JR, Briggs HA, et al. Absolute quantification by droplet digital PCR versus analog real-time PCR. Nat Methods. 2013; 10: 1003–1005. doi: 10.1038/nmeth.2633
    • (2013) Nat Methods , vol.10 , pp. 1003-1005
    • Hindson, C.M.1    Chevillet, J.R.2    Briggs, H.A.3
  • 16
    • 85033680471 scopus 로고    scopus 로고
    • Droplet-based digital PCR and next generation sequencing for monitoring circulating tumor DNA: a cancer diagnostic perspective
    • Postel M, Roosen A, Laurent-Puig P, et al. Droplet-based digital PCR and next generation sequencing for monitoring circulating tumor DNA: a cancer diagnostic perspective. Expert Rev Mol. Diagn. 2018; 18: 7–17. doi: 10.1080/14737159.2018.1400384
    • (2018) Expert Rev Mol. Diagn , vol.18 , pp. 7-17
    • Postel, M.1    Roosen, A.2    Laurent-Puig, P.3
  • 17
    • 85046353241 scopus 로고    scopus 로고
    • Detection and quantification of HDR and NHEJ induced by genome editing at endogenous gene loci using droplet digital PCR
    • Karlin-Neumann G., Bizouarn F., (eds), New York, NY: Springer New York, [Internet]. Available from:,. In:, editors.,. p
    • Miyaoka Y, Mayerl SJ, Chan AH, et al. Detection and quantification of HDR and NHEJ induced by genome editing at endogenous gene loci using droplet digital PCR. In: Karlin-Neumann G, Bizouarn F, editors. Digit. PCR methods Protoc. New York, NY: Springer New York; 2018. p. 349–362. [Internet]. Available from: https://doi.org/10.1007/978-1-4939-7778-9_20.
    • (2018) Digit. PCR methods Protoc , pp. 349-362
    • Miyaoka, Y.1    Mayerl, S.J.2    Chan, A.H.3
  • 18
    • 85086776584 scopus 로고    scopus 로고
    • Specific primers and probes for detection 2019 novel coronavirus [Internet]. 2020 [cited 2020 Apr 10]. Available from
    • National Institute For viral Disease Control and prevention of PRC. Specific primers and probes for detection 2019 novel coronavirus [Internet]. 2020 [cited 2020 Apr 10]. Available from: http://www.chinaivdc.cn/kyjz/202001/t20200121_211337.html.
  • 19
    • 77954605045 scopus 로고    scopus 로고
    • Validation of laboratory-developed molecular assays for infectious diseases
    • Burd EM., Validation of laboratory-developed molecular assays for infectious diseases. Clin Microbiol Rev. 2010; 23: 550–576. doi: 10.1128/CMR.00074-09
    • (2010) Clin Microbiol Rev , vol.23 , pp. 550-576
    • Burd, E.M.1
  • 20
    • 85081995497 scopus 로고    scopus 로고
    • Coronavirus disease (COVID-19): spectrum of CT findings and temporal progression of the disease
    • Li M, Lei P, Zeng B, et al. Coronavirus disease (COVID-19): spectrum of CT findings and temporal progression of the disease. Acad Radiol. 2020: 1–6. https://doi.org/10.1016/j.acra.2020.03.003.
    • (2020) Acad Radiol , pp. 1-6
    • Li, M.1    Lei, P.2    Zeng, B.3


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.