Isolation of HCV neutralizing antibodies by yeast display

Methods in Molecular Biology

Volumn 1911, Issue , 2019, Pages 395-419

  Keck, Zhen Yong ^a   Wang, Yong ^a   Lau, Patrick ^a   Foung, Steven K H ^a  

^a STANFORD UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Hepatitis C virus; Human monoclonal antibody isolation; Mutant antigen selection; Virus neutralization; Yeast surface display

Indexed keywords

COMPLEMENTARY DNA; GLYCINE; MONOCLONAL ANTIBODY; NEUTRALIZING ANTIBODY; SERINE; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY; HEPATITIS C ANTIBODY;

ANTIBODY ISOLATION; ANTIGEN BINDING; BINDING AFFINITY; DNA LIBRARY; GENE AMPLIFICATION; HEPATITIS C VIRUS; MEMORY CELL; MUTANT; NONHUMAN; POLYMERASE CHAIN REACTION; PURIFICATION; YEAST; B LYMPHOCYTE; BLOOD; CELL SURFACE DISPLAY; DEVICES; ENZYME LINKED IMMUNOSORBENT ASSAY; GENETIC TRANSFORMATION; GENETICS; HEPACIVIRUS; HEPATITIS C; HUMAN; IMMUNOLOGY; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR CLONING; PEPTIDE LIBRARY; PROCEDURES; SACCHAROMYCES CEREVISIAE; VIROLOGY;

ANTIBODIES, NEUTRALIZING; B-LYMPHOCYTES; CELL SURFACE DISPLAY TECHNIQUES; CLONING, MOLECULAR; ENZYME-LINKED IMMUNOSORBENT ASSAY; HEPACIVIRUS; HEPATITIS C; HEPATITIS C ANTIBODIES; HUMANS; PEPTIDE LIBRARY; SACCHAROMYCES CEREVISIAE; SINGLE-CHAIN ANTIBODIES; TRANSFORMATION, GENETIC;

EID: 85059242341     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4939-8976-8_27     Document Type: Chapter

References (14)

1. Boder ET, Wittrup KD (1997) Yeast surface display for screening combinatorial polypep-tide libraries. Nat Biotechnol 15:553–557
2. Bidlingmaier S, Su Y, Liu B (2015) Combining phage and yeast cell surface antibody display to identify novel cell type-selective internalizing human monoclonal antibodies. Methods Mol Biol 1319:51–63
3. Miller KD, Pefaur NB, Baird CL (2008) Construction and screening of antigen targeted immune yeast surface display antibody libraries. Curr Protoc Cytom Chapter 4:Unit 4 7
4. Sheehan J, Marasco WA (2015) Phage and yeast display. Microbiol Spectr 3:AID-0028-2014
5. Van Deventer JA, Wittrup KD (2014) Yeast surface display for antibody isolation: library construction, library screening, and affinity maturation. Methods Mol Biol 1131:151–181
6. Chao G, Lau WL, Hackel BJ, Sazinsky SL, Lippow SM, Wittrup KD (2006) Isolating and engineering human antibodies using yeast surface display. Nat Protoc 1:755–768
7. Feldhaus MJ, Siegel RW, Opresko LK, Coleman JR, Feldhaus JM, Yeung YA et al (2003) Flow-cytometric isolation of human antibodies from a nonimmune Saccharomyces cerevisiae surface display library. Nat Biotechnol 21:163–170
8. Feldhaus MJ, Siegel RW (2004) Yeast display of antibody fragments: a discovery and characterization platform. J Immunol Methods 290:69–80
9. Perkins S, Foung SK (1995) Stabilizing antibody secretion of human Epstein Barr virus-activated B-lymphocytes with hybridoma formation by electrofusion. Methods Mol Biol 48:295–307
10. Keck ZY, Xia J, Wang Y, Wang W, Krey T, Prentoe J et al (2012) Human monoclonal antibodies to a novel cluster of conformational epitopes on HCV E2 with resistance to neutralization escape in a genotype 2a isolate. PLoS Pathog 8:e1002653
11. Keck ZY, Saha A, Xia J, Wang Y, Lau P, Krey T et al (2011) Mapping a region of hepatitis C virus E2 that is responsible for escape from neutralizing antibodies and a core CD81-binding region that does not tolerate neutralization escape mutations. J Virol 85:10451–10463
12. Hadlock KG, Lanford RE, Perkins S, Rowe J, Yang Q, Levy S et al (2000) Human monoclonal antibodies that inhibit binding of hepatitis C virus E2 protein to CD81 and recognize conserved conformational epitopes. J Virol 74:10407–10416
13. Smith K, Garman L, Wrammert J, Zheng NY, Capra JD, Ahmed R et al (2009) Rapid generation of fully human monoclonal antibodies specific to a vaccinating antigen. Nat Protoc 4:372–384
14. Razai A, Garcia-Rodriguez C, Lou J, Geren IN, Forsyth CM, Robles Y et al (2005) Molecular evolution of antibody affinity for sensitive detection of botulinum neurotoxin type A. J Mol Biol 351:158–169