Human skin and microbial flora

Microbiology of Wounds

Volumn , Issue , 2010, Pages 59-81

  Cooper, Rose A ^a   Percival, Steven L ^b,c  

^a CARDIFF METROPOLITAN UNIVERSITY   (United Kingdom)

^b WEST VIRGINIA UNIVERSITY SCHOOL OF MEDICINE   (United States)

^c Advanced Medical Solutions   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 85054621890     PISSN: None     EISSN: None     Source Type: Book    
DOI: 10.1201/9781420079944     Document Type: Chapter

References (93)

1. Wysocki, A.B. (2002) Evaluating and managing open skin wounds: Colonization versus infection. AACN Clin Issues 13(3):382-397.
2. Chiller, K., Selkin, B.A., and Murakawa, G.J. (2001) Skin microflora and bacterial infections of the skin. J Invest Dermatol Symp Proc 6:170-174.
3. Baumann, L., Weisberg, E., and Percival, S.L. (2009) Skin aging and microbiology. In: Microbiology and Aging: Clinical Manifestations. S.L. Pervical, editor. Springer, Humana Press, NY.
4. Savey, A., Fleurette, J., and Salle, B.L. (1992) An analysis of the microbial flora of premature neonates. J Hosp Infect 21(4):275-289.
5. Aly, R., and Maibach, H.I. (1977) Aerobic microbial flora of intertrigenous skin. Appl Environ Microbiol 33(1):97-100.
6. Sarkany, I., and Gaylarde, C.C. (1968) Bacterial colonisation of the skin of the newborn. J Pathol Bacteriol 95(1):115-122.
7. Schröder, J.M., Reich, K., Kabashima, K., Liu, F.T., Romani, N., Metz, M., Kerstan, A., Lee, P.H., Loser, K., Schön, M.P., Maurer, M., Stoitzner, P., Beissert, S., Tokura, Y., and Gallo, R.L. (2006) Who is really in control of skin immunity under physiological circumstances-Lymphocytes, dendritic cells or keratinocytes? Exp Dermatol 15(11):913-929.
8. Nizet, V., Ohtake, T., Lauth, X., Trowbridge, J., Rudisill, J., Dorschner, R.A., Pestonjamasp, V., Piraino, J., Hutter, K., and Gallo, R.L. (2001) Innate antimicrobial peptide protects the skin from invasive bacterial infection. Nature 414:454-457.
9. Schröder, J.M., and Harder, J. (2006) Innate antimicrobial peptides in the skin. Med Sci (Paris) 22(2):153-157.
10. Harder, J., and Schröder, J.M. (2005) Antimicrobial peptides in human skin. Chem Immunol Allergy 86:22-41.
11. Marples, R.R. (1982) Sex, constancy, and skin bacteria. Arch Dermatol 272:317-320.
12. Wilburg, J., Kasprowicz, A., and Heczko, P.B. (1984) Composition of normal bacterial flora of human skin in relation to the age and sex of examined persons. Przegl Dermatol 71(6):551-557.
13. Larson, E.L., Cronquist, A.B., Whittier, S., Lai, L., Lyle, C.T., and Latta, P.D. (2009) Differences in skin flora between inpatients and chronically ill outpatients. Heart and Lung 29:298-305.
14. Rebel, G., Pillsbury, D.M., Phalle, G., de Saint, M., and Ginsberg, D. (1950) Factors affecting the rapid disappearance of bacteria placed on the normal flora. J Invest Dermatol 14:247-263.
15. Price, P.B. (1938) The bacteriology of normal skin: A new quantitative test applied to a study of the bacterial flora and the disinfectant action of mechanical cleansing. J Infect Dis 63:301-318.
16. Somerville-Millar, D.A., and Noble, W.C. (1974) Resident and transient bacteria of the skin. J Cutan Pathol 1(6):260-264.
17. Williamson, P. (1965) Quantitative estimation of cutaneous flora. In: Maibach, H.I., and Hildick-Smith, G., editors. Skin Bacteria and Their Role in Infection. New York:McGraw-Hill, p. 3.
18. Marples, M.J. (1974) The Ecology of Human Skin. 1965. Thomas CC, Springfield, IL; Noble, W.C., and Somerville, C.A. Microbiology of Human Skin, pp. 50-76, 131, 212. 1974. WB Saunders, Philadelphia.
19. Noble, W.C. and Somerville, C.A. (1974) Microbiology of Human Skin. Philadelphia:WB Saunders, pp. 50-76, 131, 212.
20. Kligman, A.M. (1965) The bacteriology of normal skin. In: Maibach, H.I., and Hildick-Smith, G., editors. Skin Bacteria and Their Role in Infection. New York: McGraw-Hill, pp. 13-31.
21. Williamson, P., and Kligman, A.M. (1965) A new method for the quantitative investigation of cutaneous bacteria. J Invest Dermatol 45(6):498-503.
22. Kligman, A.M. (1965) The bacteriology of normal skin. In: Maibach, H.I., and Hildick-Smith, G., editors. Skin Bacteria and Their Role in Infection. New York: McGraw-Hill, pp. 13-31.
23. Paulino, L.C., Tseng, C.H., Strober, B.E., and Blaser, M.J. (2006) Molecular analysis of fungal microbiota in samples from healthy human skin and psoriatic lesions. J Clin Microbiol 44(8):2933-2941.
24. Bibel, D.J., and Lovell, D.J. (1976) Skin flora maps: A tool in the study of cutaneous ecology. J Invest Dermatol 67(2):265-269.
25. Noble, W.C. (1980) Carriage of micro-organisms on the skin. In: Newsom, S.W.B., and Caldwell, A.D.S., editors. Problems in the Control of Hospital Infection. London: Royal Society of Medicine, pp. 7-10.
26. Mackowiak, P.A. (1982) The normal microbial flora. N Engl J Med 307:83-93.
27. Gao, Z., Tseng, C.H., Pei, Z. et al. (2007) Molecular analysis of human forearm superficial skin bacterial biota. Proc Natl Acad Sci USA 104:2927-2932.
28. Somerville, D.A., and Noble, W.C. (1973) Microcolony size of microbes on human skin. J Med Microbiol 6(3):323-328.
29. Montes, L.F. (1970) Systemic abnormalities and the intracellular site of infections of the stratum corneum. JAMA 31:1469-1472.
30. Elsner, P. (2006) Antimicrobials and the skin physiological and pathological flora. In:Hipler, U.-C., and Elsner, P., editors. Efficiency of Biofunctional Textiles and the Skin. Current Problems in Dermatology. Basel: Karger, pp. 35-41.
31. Bojar, R.A., and Holland, K.T. (2002) The human cutaneous microbiota and factors controlling colonisation. World J Microbiol Biotechnol 18:889-903.
32. Seifert, H., Dijkshoorn, L., Gerner-Smidt, P., Pelzer, N., Tjernberg, I., and Vaneechoutte, M. (1997) Distribution of Acinetobacter species on human skin: Comparison of phenotypic and genotypic identification methods. J Clin Microbiol 53:2819-2825.
33. Leyden, J.J., McGinley, K.J., Nordstrom, K.M., and Webster, G.F. (1987) Skin microflora. J Invest Dermatol 88:65s-72s.
34. Kaźmierczak, A.K., Szarapińska-Kwaszewska, J.K., and Szewczyk, E.M. (2005) Opportunistic coryneform organisms-Residents of human skin. Pol J Microbiol 54(1):27-35.
35. Webster, G.F. (2007) Skin microecology: The old and the new. Arch Dermatol 143(1):105-106.
36. McGinley, K.J., Leyden, J.J., Marples, R.R., and Kligman, A.M. (1975) Quantitative microbiology of the scalp in non-dandruff, dandruff, and seborrheic dermatitis. J Invest Dermatol 64(6):401-405.
37. Chamberlain, A.N., Halablab, M.A., Gould, D.J., and Miles, R.J. (1997) Distribution of bacteria on hands and the effectiveness of brief and thorough decontamination procedures using non-medicated soap. Zentralbl Bakteriol 285(4):565-575.
38. Somerville, D.A. (1980) The normal flora of the skin in different age groups. Br J Dermatol 81:248-258.
39. Selwyn, S. (1980) Microbiology and ecology of human skin. Practitioner 224:1059-1062.
40. Marples, M.J. (1969) The normal flora of the human skin. Br J Dermatol 81(suppl. 1):2-13.
41. LeFrock, J.L., Ellis, C.A., and Weinstein, L. (1979) The impact of hospitalization on the aerobic fecal microflora. Am J Med Sci 277:269-274.
42. Larson, E.L. (1981) Persistent carriage of Gram-negative bacteria on hands. Am J Infect Control 9:112-119.
43. Larson, E.L., McGinley, K.J., Foglia, A.R., Talbot, G.H., and Leyden, J.J. (1986) Composition and antimicrobial resistance of skin flora in hospitalized and healthy adults. J Clin Microbiol 23:604-608.
44. Seifert, H., Dijkshoorn, L., Gerner-Smidt, P., Pelzer, N., Tjernberg, I., and Vaneechoutte, M. (1997) Distribution of Acinetobacter species on human skin: Comparison of phenotypic and genotypic identification methods. J Clin Microbiol 53:2819-2825.
45. Rennie, R.P., Jones, R.N., and Mutnick, A.H.; SENTRY Program Study Group (North America). (2003) Occurrence and antimicrobial susceptibility patterns of pathogens isolated from skin and soft tissue infections: Report from the SENTRY Antimicrobial Surveillance Program (United States and Canada, 2000). Diagn Microbiol Infect Dis 45(4):287-293.
46. Bek-Thomsen, M., Lomholt, H.B., and Kilian, M. (2008) Acne is not associated with yet-uncultured bacteria. J Clin Microbiol 46(10):3355-3360.
47. Dekio, I., Hayashi, H., Sakamoto, M., Kitahara, M., Nishikawa, T., Suematsu, M., and Benno, Y. (2005) Detection of potentially novel bacterial components of the human skin microbiota using culture-independent molecular profiling. J Med Microbiol 54:1231-1238.
48. Grice, E.A., Kong, H.H., Renaud, G., and Young, A.C. (2008) NISC Comparative Sequencing Program, Bouffard, G.G., Blakesley, R.W., Wolfsberg, T.G., Turner, M.L., Segre, J.A. A diversity profile of the human skin microbiota. Genome Res 18(7):1043-1050.
49. Barton, M.D., McBain, A.J., and Gilbert, P. (2007) Molecular and cultural analyses of human skin. Proceedings of the 107th Meeting on the American Society for Microbiology. May 21-25, Toronto, Canada
50. Dekio, I., Sakamoto, M., Hayashi, H., Amagai, M., Suematsu, M., and Benno, Y. (2007) Characterization of skin microbiota in patients with atopic dermatitis and in normal subjects using 16S rRNA gene-based comprehensive analysis. J Med Microbiol 56(Pt 12):1675-1683.
51. Gao, Z., Tseng, C.H., Pei, Z. et al. (2007) Molecular analysis of human forearm superficial skin bacterial biota. Proc Natl Acad Sci USA 104:2927-2932.
52. Sugita, T., Suzuki, M., Goto, S., Nishikawa, A., Hiruma, M., Yamazaki, T., and Makimura, K. (2009) Quantitative analysis of the cutaneous Malassezia microbiota in 770 healthy Japanese by age and gender using a real-time PCR assay. Med Mycol 21:1-5.
53. Suzuki, T., Kawamura, Y., Uno, T., Ohashi, Y., and Ezaki, T. (2005) Prevalence of Staphylococcus epidermidis strains with biofilm-forming ability in isolates from conjunctiva and facial skin. Am J Ophthalmol 140(5):844-850.
54. Funke, G., von Graevenitz, A., Clarridge, J.E., and Bernard, K.A. (1997) Clinical microbiology of coryneform bacteria. Clin Micro Rev 10(1):125-159.
55. Vuong, C., and Otto, M. (2002) Staphylococcus epidermidis infections. Microbes and Infection 4:481-489.
56. Harmory, B.H., and Parisi, J.T. (1987) Staphylococcus epidermidis: A significant nosocomial pathogen. J Infect Control 15:59-74.
57. Fekety, F.R. Jr. (1964) The epidemiology and prevention of staphylococcal infection. Medicine 43:593-613.
58. Nagase, N., Sasaki, A., Yamashita, K., Shimizu, A., Wakita, Y., Kitai, S., and Kawano, J. (2002) Isolation and species distribution of staphylococci from animal and human skin. J Vet Med Sci 64(3):245-250.
59. Barth, J.H. (1987) Nasal carriage of staphylococci and streptococci. Int J Dermatol 26:24-26.
60. Nobel, W.C. (1981) Microbiology of Human Skin. London: Lloyd-Luke.
61. Kloos, W.E., and Bannerman, T.L. (1994) Update on the clinical significance of coagulase- negative staphylococci. Clin Rev Micro 7(1):117-140.
62. O’Gara, J.P., and Humphreys, H. (2001) Staphylococcus epidermidis biofilms:Importance and implications. J Med Micro 50:582-587.
63. Foster, T.J. 2004. The Staphylococcus aureus “superbug.” J Clin Invest 114:1693-1696.
64. Bjarnsholt, T., Kirketerp-Møller, K., Kristiansen, S., Phipps, R., Nielsen, A.K., Jensen, P.Ø., Høiby, N., and Givskov, M. (2007) Silver against Pseudomonas aeruginosa biofilms. APMIS 115(8):921-928.
65. Taplin, D., Rebell, A.M., and Zaiab, N. The human skin as a source of Mima-Herella infections. JAMA 186:166-168.
66. Seifert, H., Dijkshoorn, L., Gerner-Smidt, P., Pelzer, N., Tjernberg, I., and Vaneechoutte, M. (1997) Distribution of Acinetobacter species on human skin: Comparison of phenotypic and genotypic identification methods. J Clin Microbiol 53:2819-2825.
67. Paulino, L.C., Tseng, C.-H., Strober, B.E., and Blaser, M.J. (2006) Molecular analysis of fungal microbiota in samples from healthy human skin and psoriatic lesions. J Clin Microbiol 44:2933-2941.
68. Faergemann, J., Aly, R., and Maibach, H.I. (1983) Quantitative variations in distribution of Pitryosporum orbiculare on clinically normal skin. Acta Derm Venereol 63:346-348.
69. Gupta, A.K., and Kohli, Y. (2004) Prevalence of Malassezia species on various body sites in clinically healthy subjects representing different age groups. Med Mycol 10:125-159.
70. Barak, O., Treat, J.R., and James, W.D. (2005) Antimicrobial peptides: Effectors of innate immunity in the skin. Adv Dermatol 21:357-374.
71. Chiller, K., Selkin, B.A., and Murakawa, G.J. (2001) Skin microflora and bacterial infections of the skin. J Invest Dermatol Symp Proc 6:170-174.
72. Paulino, L.C., Tseng, C.H., Strober, B.E., and Blaser, M.J. (2006) Molecular analysis of fungal microbiota in samples from healthy human skin and psoriatic lesions. J Clin Microbiol 44(8):2933-2941.
73. Fredricks, D.N. (2001) Microbial ecology of human skin in health and disease. J Invest Dermatol Symp Proc 6:167-169.
74. Hadaway, L.C. (2003) Skin flora and infection. J Infusional Nursing 26:44-48.
75. Hadaway, L.C. (2005) Skin flora: Unwanted dead or alive. Nursing 35(7):20.
76. Roth, R.R., and James, W.D. (1989) Microbiology of the skin: Resident flora, ecology, infection. J Am Acad Dermatol 20:367-390.
77. Laube, S., and Farrell, A.M. (2002) Bacterial skin infections in the elderly: Diagnosis and treatment. Drugs and Aging 19:331-342.
78. Cogen, A.L., Nizet, V., and Gallo, R.L. (2007) Staphylococcus epidermidis functions as a component of the skin innate immune system by inhibiting the pathogen Group A Streptococcus. J Invest Dermatol 27:S131.
79. Lyon, G.J., and Novick, R.P. (2004) Peptide signaling in Staphylococcus aureus and other Gram-positive bacteria. Peptides 25:1389-1403.
80. von Eiff, C., Becker, K., Machka, K., Stammer, H., and Peters, G. (2001) Nasal carriage as a source of Staphylococcus aureus bacteremia. Study Group. N Engl J Med 344:11-16.
81. von Eiff, C., Peters, G., and Heilmann, C. (2002) Pathogenesis of infections due to coagulase negative staphylococci. Lancet Infect Dis 2:677-685.
82. Mainous, A.G. 3rd, Hueston, W.J., Everett, C.J., and Diaz, V.A. (2006) Nasal carriage of Staphylococcus aureus and methicillin-resistant S. aureus in the United States, 2001-2002. Ann Fam Med 4:132-137.
83. Iwatsuki, K., Yamasaki, O., Morizane, S., and Oono, T. (2006) Staphylococcal cutaneous infections: Invasion, evasion and aggression. J Dermatol Sci 42:203-214.
84. Bokarewa, M.I., Jin, T., and Tarkowski, A. (2006) Staphylococcus aureus: Staphylokinase. Int J Biochem Cell Biol 38(4):504-509.
85. Wichmann, S., Wirsing von Koenig, C.H., Becker-Boost, E., and Finger, H. (1985) Group JK corynebacteria in skin flora of healthy persons and patients. Eur J Clin Microbiol 4:502-504.
86. Kaźmierczak, A.K., and Szewczyk, E.M. (2005) Bacteria forming a resident flora of the skin as a potential source of opportunistic infections. Pol J Microbiol 54(1):27-35.
87. Kaźmierczak, A.K., Szarapińska-Kwaszewska, J.K., and Szewczyk, E.M. (2005) Opportunistic coryneform organisms: Residents of human skin. Pol J Microbiol 54(1):27-35.
88. Brüggemann, H., Henne, A., Hoster, F., Liesegang, H., Wiezer, A., Strittmatter, A., Hujer, S., Dürre, P., and Gottschalk, G. (2004) The complete genome sequence of Propionibacterium acnes, a commensal of human skin. Science 305:671-673.
89. Goodyear, H.M., Watson, P.J., Egan, S.A., Price, E.H., Kenny, P.A., and Harper, J.I. (1993) Skin microflora of atopic eczema in first time hospital attenders. Clin Exp Dermatol 18(4):300-304.
90. Cogen, A.L., Nizet, V., and Gallo, R.L. (2008) Skin microbiota: A source of disease or defence? Br J Dermatol 158:442-455.
91. Bickers, D.R., and Athar, M. (2006) Oxidative stress in the pathogenesis of skin disease. J Invest Dermatol 126(12):2565-2575.
92. Platzek, T., Lang, C., Grohmann, G., Gi, U.S., and Baltes, W. (1999) Formation of a carcinogenic aromatic amine from an azo dye by human skin bacteria in vitro. Hum Exp Toxicol 18(9):552-559.
93. Papacostas, G., and Gate, J. eds. (1928) Les associations microbiennes, leurs applications therapeutiques. Paris: Doin.