High-throughput cellular screening of engineered nuclease activity using the single-strand annealing assay and luciferase reporter

Methods in Molecular Biology

Volumn 1114, Issue , 2014, Pages 339-352

  Cradick, Thomas J ^a   Antico, Christopher J ^a   Bao, Gang ^a  

^a GEORGIA INSTITUTE OF TECHNOLOGY   (United States)

Author keywords

Engineered nucleases; Luciferase; Single strand annealing; Transcription activated like effector nuclease; Zinc finger nuclease

Indexed keywords

NUCLEASE; TRANSCRIPTION ACTIVATED LIKE EFFECTOR NUCLEASE; UNCLASSIFIED DRUG; ZINC FINGER NUCLEASE; LUCIFERASE;

ARTICLE; CELL CULTURE; CONTROLLED STUDY; CRISPR CAS SYSTEM; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME ENGINEERING; GENETIC TRANSFECTION; HIGH THROUGHPUT SCREENING; IN VITRO STUDY; LUCIFERASE ASSAY; PLASMID; PRIORITY JOURNAL; SIGNAL TRANSDUCTION; SINGLE STRAND ANNEALING ASSAY; TISSUE CULTURE; GENE EXPRESSION ASSAY; STOP CODON;

DNA CLEAVAGE; ENDONUCLEASES; GENE EXPRESSION; GENES, REPORTER; HEK293 CELLS; HIGH-THROUGHPUT SCREENING ASSAYS; HUMANS; LUCIFERASES; ZINC FINGERS;

EID: 85053645935     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-761-7_22     Document Type: Article

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