-
1
-
-
84886022552
-
Immune Monitoring Design within the Developmental Pipeline for an Immunotherapeutic or Preventive Vaccine
-
Morrow, W.J.W., Sheikh, N.A., Schmidt, C.S., Davies, D.H., Eds; Wiley-Blackwell: Hoboken, NJ, USA
-
Janetzki, S.; Romero, P.; Roederer, M.; Bolton, D.; Jandus, C. Immune Monitoring Design within the Developmental Pipeline for an Immunotherapeutic or Preventive Vaccine. In Vaccinology: Principles and Practice; Morrow, W. J. W., Sheikh, N. A., Schmidt, C. S., Davies, D. H., Eds; Wiley-Blackwell: Hoboken, NJ, USA, 2012; pp. 419-440.
-
(2012)
Vaccinology: Principles and Practice
, pp. 419-440
-
-
Janetzki, S.1
Romero, P.2
Roederer, M.3
Bolton, D.4
Jandus, C.5
-
2
-
-
78649740257
-
Isolation and preservation of peripheral blood mononuclear cells for analysis of islet antigen-reactive cell responses: Position statement of the T-cell workshop committee of the immunology of t diabetes society
-
Mallone, R.; Mannering, S. I.; Brooks-Worrell, B. M.; Durinovic-Bello, I.; Cilio, C. M.; Wong, F. S.; Schloot, N. C. Isolation and preservation of peripheral blood mononuclear cells for analysis of islet antigen-reactive cell responses: Position statement of the T-cell workshop committee of the immunology of t diabetes society. Clin. Exp. Immunol. 2011, 163, 33-49.
-
(2011)
Clin. Exp. Immunol
, vol.163
, pp. 33-49
-
-
Mallone, R.1
Mannering, S.I.2
Brooks-Worrell, B.M.3
Durinovic-Bello, I.4
Cilio, C.M.5
Wong, F.S.6
Schloot, N.C.7
-
3
-
-
34147176167
-
Defining blood processing parameters for optimal detection of cryopreserved antigen-specific responses for HIV vaccine trials
-
Bull, M.; Lee, D.; Stucky, J.; Chiu, Y. L.; Rubin, A.; Horton, H.; McElrath, M. J. Defining blood processing parameters for optimal detection of cryopreserved antigen-specific responses for HIV vaccine trials. J. Immunol. Methods 2007, 322, 57-69.
-
(2007)
J. Immunol. Methods
, vol.322
, pp. 57-69
-
-
Bull, M.1
Lee, D.2
Stucky, J.3
Chiu, Y.L.4
Rubin, A.5
Horton, H.6
McElrath, M.J.7
-
4
-
-
33846813761
-
Enhanced rates and magnitude of immune responses detected against an hiv vaccine: Effect of using an optimized process for isolating pbmc
-
Kierstead, L. S.; Dubey, S.; Meyer, B.; Tobery, T. W.; Mogg, R.; Fernandez, V. R.; Long, R.; Guan, L.; Gaunt, C.; Collins, K.; et al. Enhanced rates and magnitude of immune responses detected against an hiv vaccine: Effect of using an optimized process for isolating pbmc. AIDS Res. Hum. Retroviruses 2007, 23, 86-92.
-
(2007)
AIDS Res. Hum. Retroviruses
, vol.23
, pp. 86-92
-
-
Kierstead, L.S.1
Dubey, S.2
Meyer, B.3
Tobery, T.W.4
Mogg, R.5
Fernandez, V.R.6
Long, R.7
Guan, L.8
Gaunt, C.9
Collins, K.10
-
5
-
-
77954384687
-
Critical parameters in blood processing for t-cell assays: Validation on ELISPOT and tetramer platforms
-
Afonso, G.; Scotto, M.; Renand, A.; Arvastsson, J.; Vassilieff, D.; Cilio, C. M.; Mallone, R. Critical parameters in blood processing for t-cell assays: Validation on ELISPOT and tetramer platforms. J. Immunol. Methods 2010, 359, 28-36.
-
(2010)
J. Immunol. Methods
, vol.359
, pp. 28-36
-
-
Afonso, G.1
Scotto, M.2
Renand, A.3
Arvastsson, J.4
Vassilieff, D.5
Cilio, C.M.6
Mallone, R.7
-
6
-
-
79952320073
-
Shipping blood to a central laboratory in multicenter clinical trials: Effect of ambient temperature on specimen temperature, and effects of temperature on mononuclear cell yield, viability and immunologic function
-
Olson, W. C.; Smolkin, M. E.; Farris, E. M.; Fink, R. J.; Czarkowski, A. R.; Fink, J. H.; Chianese-Bullock, K. A.; Slingluff, C. L., Jr. Shipping blood to a central laboratory in multicenter clinical trials: Effect of ambient temperature on specimen temperature, and effects of temperature on mononuclear cell yield, viability and immunologic function. J. Translat. Med. 2011, 9, 26.
-
(2011)
J. Translat. Med
, vol.9
, pp. 26
-
-
Olson, W.C.1
Smolkin, M.E.2
Farris, E.M.3
Fink, R.J.4
Czarkowski, A.R.5
Fink, J.H.6
Chianese-Bullock, K.A.7
Slingluff, C.L.8
-
7
-
-
59249095471
-
Delayed processing of blood increases the frequency of activated CD11b+ CD15+ granulocytes which inhibit T-cell function
-
McKenna, K. C.; Beatty, K. M.; Vicetti Miguel, R.; Bilonick, R. A. Delayed processing of blood increases the frequency of activated CD11b+ CD15+ granulocytes which inhibit T-cell function. J. Immunol. Methods 2009, 341, 68-75.
-
(2009)
J. Immunol. Methods
, vol.341
, pp. 68-75
-
-
McKenna, K.C.1
Beatty, K.M.2
Vicetti Miguel, R.3
Bilonick, R.A.4
-
8
-
-
84877806022
-
Serum-free freezing media support high cell quality and excellent ELISPOT assay performance across a wide variety of different assay protocols
-
Filbert, H.; Attig, S.; Bidmon, N.; Renard, B. Y.; Janetzki, S.; Sahin, U.; Welters, M. J.; Ottensmeier, C.; van der Burg, S. H.; Gouttefangeas, C.; et al. Serum-free freezing media support high cell quality and excellent ELISPOT assay performance across a wide variety of different assay protocols. Cancer Immunol. Immunother. 2013, 62, 615-627.
-
(2013)
Cancer Immunol. Immunother
, vol.62
, pp. 615-627
-
-
Filbert, H.1
Attig, S.2
Bidmon, N.3
Renard, B.Y.4
Janetzki, S.5
Sahin, U.6
Welters, M.J.7
Ottensmeier, C.8
van der Burg, S.H.9
Gouttefangeas, C.10
-
9
-
-
0034834610
-
Development and validation of a gamma interferon ELISPOT assay for quantitation of cellular immune responses to varicella-zoster virus
-
Smith, J. G.; Liu, X.; Kaufhold, R. M.; Clair, J.; Caulfield, M. J. Development and validation of a gamma interferon ELISPOT assay for quantitation of cellular immune responses to varicella-zoster virus. Clin. Diagnost. Lab. Immunol. 2001, 8, 871-879.
-
(2001)
Clin. Diagnost. Lab. Immunol
, vol.8
, pp. 871-879
-
-
Smith, J.G.1
Liu, X.2
Kaufhold, R.M.3
Clair, J.4
Caulfield, M.J.5
-
10
-
-
77449098774
-
Performance of serum-supplemented and serum free media in ifngamma ELISPOT assays for human T-cells
-
Janetzki, S.; Price, L.; Britten, C. M.; van der Burg, S. H.; Caterini, J.; Currier, J. R.; Ferrari, G.; Gouttefangeas, C.; Hayes, P.; Kaempgen, E.; et al. Performance of serum-supplemented and serum free media in ifngamma ELISPOT assays for human T-cells. Cancer Immunol. Immunother. 2010, 59, 609-618.
-
(2010)
Cancer Immunol. Immunother
, vol.59
, pp. 609-618
-
-
Janetzki, S.1
Price, L.2
Britten, C.M.3
van der Burg, S.H.4
Caterini, J.5
Currier, J.R.6
Ferrari, G.7
Gouttefangeas, C.8
Hayes, P.9
Kaempgen, E.10
-
11
-
-
34250339664
-
Establishing acceptance criteria for cell-mediated-immunity assays using frozen peripheral blood mononuclear cells stored under optimal and suboptimal conditions
-
Smith, J. G.; Joseph, H. R.; Green, T.; Field, J. A.; Wooters, M.; Kaufhold, R. M.; Antonello, J.; Caulfield, M. J. Establishing acceptance criteria for cell-mediated-immunity assays using frozen peripheral blood mononuclear cells stored under optimal and suboptimal conditions. Clin. Vaccine Immunol. 2007, 14, 527-537.
-
(2007)
Clin. Vaccine Immunol
, vol.14
, pp. 527-537
-
-
Smith, J.G.1
Joseph, H.R.2
Green, T.3
Field, J.A.4
Wooters, M.5
Kaufhold, R.M.6
Antonello, J.7
Caulfield, M.J.8
-
12
-
-
77952533489
-
The effect of apoptotic cells on virus-specific immune responses detected using IFNγ ELISPOT
-
Lenders, K.; Ogunjimi, B.; Beutels, P.; Hens, N.; van Damme, P.; Berneman, Z. N.; van Tendeloo, V. F.; Smits, E. L. The effect of apoptotic cells on virus-specific immune responses detected using IFNγ ELISPOT. J. Immunol. Methods 2010, 357, 51-54.
-
(2010)
J. Immunol. Methods
, vol.357
, pp. 51-54
-
-
Lenders, K.1
Ogunjimi, B.2
Beutels, P.3
Hens, N.4
van Damme, P.5
Berneman, Z.N.6
van Tendeloo, V.F.7
Smits, E.L.8
-
13
-
-
84255160968
-
Preculture of pbmcs at high cell density increases sensitivity of T-cell responses, revealing cytokine release by CD28 superagonist TGN1412
-
Romer, P. S.; Berr, S.; Avota, E.; Na, S. Y.; Battaglia, M.; ten Berge, I.; Einsele, H.; Hunig, T. Preculture of pbmcs at high cell density increases sensitivity of T-cell responses, revealing cytokine release by CD28 superagonist TGN1412. Blood 2011, 118, 6772-6782.
-
(2011)
Blood
, vol.118
, pp. 6772-6782
-
-
Romer, P.S.1
Berr, S.2
Avota, E.3
Na, S.Y.4
Battaglia, M.5
ten Berge, I.6
Einsele, H.7
Hunig, T.8
-
14
-
-
37349020262
-
Results and harmonization guidelines from two largescale international ELISPOT proficiency panels conducted by the cancer vaccine consortium (cvc/svi)
-
Janetzki, S.; Panageas, K. S.; Ben-Porat, L.; Boyer, J.; Britten, C. M.; Clay, T. M.; Kalos, M.; Maecker, H. T.; Romero, P.; Yuan, J.; et al. Results and harmonization guidelines from two largescale international ELISPOT proficiency panels conducted by the cancer vaccine consortium (cvc/svi). Cancer Immunol. Immunother. 2008, 57, 303-315.
-
(2008)
Cancer Immunol. Immunother
, vol.57
, pp. 303-315
-
-
Janetzki, S.1
Panageas, K.S.2
Ben-Porat, L.3
Boyer, J.4
Britten, C.M.5
Clay, T.M.6
Kalos, M.7
Maecker, H.T.8
Romero, P.9
Yuan, J.10
-
15
-
-
37349077911
-
The cimt-monitoring panel: A two-step approach to harmonize the enumeration of antigen-specific CD8+ t lymphocytes by structural and functional assays
-
Britten, C. M.; Gouttefangeas, C.; Welters, M. J.; Pawelec, G.; Koch, S.; Ottensmeier, C.; Mander, A.; Walter, S.; Paschen, A.; Muller-Berghaus, J.; et al. The cimt-monitoring panel: A two-step approach to harmonize the enumeration of antigen-specific CD8+ t lymphocytes by structural and functional assays. Cancer Immunol. Immunother. 2008, 57, 289-302.
-
(2008)
Cancer Immunol. Immunother
, vol.57
, pp. 289-302
-
-
Britten, C.M.1
Gouttefangeas, C.2
Welters, M.J.3
Pawelec, G.4
Koch, S.5
Ottensmeier, C.6
Mander, A.7
Walter, S.8
Paschen, A.9
Muller-Berghaus, J.10
-
16
-
-
81055146762
-
Harmonization of immune biomarker assays for clinical studies
-
Van der Burg, S. H.; Kalos, M.; Gouttefangeas, C.; Janetzki, S.; Ottensmeier, C.; Welters, M. J.; Romero, P.; Britten, C. M.; Hoos, A. Harmonization of immune biomarker assays for clinical studies. Sci. Transl. Med. 2011, 3, 108ps44.
-
(2011)
Sci. Transl. Med
, vol.3
, pp. 108
-
-
Van der Burg, S.H.1
Kalos, M.2
Gouttefangeas, C.3
Janetzki, S.4
Ottensmeier, C.5
Welters, M.J.6
Romero, P.7
Britten, C.M.8
Hoos, A.9
-
17
-
-
80555129200
-
The impact of harmonization on ELISPOT assay performance
-
Janetzki, S.; Britten, C. M. The impact of harmonization on ELISPOT assay performance. Methods Mol. Biol. 2012, 792, 25-36.
-
(2012)
Methods Mol. Biol
, vol.792
, pp. 25-36
-
-
Janetzki, S.1
Britten, C.M.2
-
18
-
-
84885418048
-
Overnight resting of pbmc changes functional signatures of antigen specific T-cell responses: Impact for immune monitoring within clinical trials
-
Kutscher, S.; Dembek, C. J.; Deckert, S.; Russo, C.; Korber, N.; Bogner, J. R.; Geisler, F.; Umgelter, A.; Neuenhahn, M.; Albrecht, J.; et al. Overnight resting of pbmc changes functional signatures of antigen specific T-cell responses: Impact for immune monitoring within clinical trials. PLoS One 2013, 8, e76215.
-
(2013)
PLoS One
, vol.8
-
-
Kutscher, S.1
Dembek, C.J.2
Deckert, S.3
Russo, C.4
Korber, N.5
Bogner, J.R.6
Geisler, F.7
Umgelter, A.8
Neuenhahn, M.9
Albrecht, J.10
-
19
-
-
77955551910
-
Response definition criteria for ELISPOT assays revisited
-
Moodie, Z.; Price, L.; Gouttefangeas, C.; Mander, A.; Janetzki, S.; Lower, M.; Welters, M. J.; Ottensmeier, C.; van der Burg, S. H.; Britten, C. M. Response definition criteria for ELISPOT assays revisited. Cancer Immunol. Immunother. 2010, 59, 1489-1501.
-
(2010)
Cancer Immunol. Immunother
, vol.59
, pp. 1489-1501
-
-
Moodie, Z.1
Price, L.2
Gouttefangeas, C.3
Mander, A.4
Janetzki, S.5
Lower, M.6
Welters, M.J.7
Ottensmeier, C.8
van der Burg, S.H.9
Britten, C.M.10
-
20
-
-
84864325057
-
T-cell assays and miata: The essential minimum for maximum impact
-
Britten, C. M.; Janetzki, S.; Butterfield, L. H.; Ferrari, G.; Gouttefangeas, C.; Huber, C.; Kalos, M.; Levitsky, H. I.; Maecker, H. T.; Melief, C. J.; et al. T-cell assays and miata: The essential minimum for maximum impact. Immunity 2012, 37, 1-2.
-
(2012)
Immunity
, vol.37
, pp. 1-2
-
-
Britten, C.M.1
Janetzki, S.2
Butterfield, L.H.3
Ferrari, G.4
Gouttefangeas, C.5
Huber, C.6
Kalos, M.7
Levitsky, H.I.8
Maecker, H.T.9
Melief, C.J.10
-
21
-
-
0036469827
-
A panel of MHC class I restricted viral peptides for use as a quality control for vaccine trial ELISPOT assays
-
Currier, J. R.; Kuta, E. G.; Turk, E.; Earhart, L. B.; Loomis-Price, L.; Janetzki, S.; Ferrari, G.; Birx, D. L.; Cox, J. H. A panel of MHC class I restricted viral peptides for use as a quality control for vaccine trial ELISPOT assays. J. Immunol. Methods 2002, 260, 157-172.
-
(2002)
J. Immunol. Methods
, vol.260
, pp. 157-172
-
-
Currier, J.R.1
Kuta, E.G.2
Turk, E.3
Earhart, L.B.4
Loomis-Price, L.5
Janetzki, S.6
Ferrari, G.7
Birx, D.L.8
Cox, J.H.9
-
22
-
-
85117871277
-
-
Lucie, FL, USA. Unpublished work
-
Santos, R.; Buying A.; Sabri, N.; Appel, J.; Yu, J.; Gringeri, A.; Bender, J.; Janetzki, S.; Pinilla, C.; Judkowski, V.; et al. Torrey Pines Institute for Molecular Studies, Port St. Lucie, FL, USA. Unpublished work, 2014.
-
(2014)
Torrey Pines Institute for Molecular Studies, Port St
-
-
Santos, R.1
Buying, A.2
Sabri, N.3
Appel, J.4
Yu, J.5
Gringeri, A.6
Bender, J.7
Janetzki, S.8
Pinilla, C.9
Judkowski, V.10
-
23
-
-
0037191792
-
Self-recognition promotes the foreign antigen sensitivity of naive T lymphocytes
-
Stefanova, I.; Dorfman, J. R.; Germain, R. N. Self-recognition promotes the foreign antigen sensitivity of naive T lymphocytes. Nature 2002, 420, 429-434.
-
(2002)
Nature
, vol.420
, pp. 429-434
-
-
Stefanova, I.1
Dorfman, J.R.2
Germain, R.N.3
-
24
-
-
58649114309
-
The molecular basis of tcr germline bias for mhc is surprisingly simple
-
Garcia, K. C.; Adams, J. J.; Feng, D.; Ely, L. K. The molecular basis of tcr germline bias for mhc is surprisingly simple. Nat. Immunol. 2009, 10, 143-147.
-
(2009)
Nat. Immunol
, vol.10
, pp. 143-147
-
-
Garcia, K.C.1
Adams, J.J.2
Feng, D.3
Ely, L.K.4
-
25
-
-
0038025412
-
T-cell adhesion lowers the threshold for antigen detection
-
Randriamampita, C.; Boulla, G.; Revy, P.; Lemaitre, F.; Trautmann, A. T-cell adhesion lowers the threshold for antigen detection. Eur. J. Immunol. 2003, 33, 1215-1223.
-
(2003)
Eur. J. Immunol
, vol.33
, pp. 1215-1223
-
-
Randriamampita, C.1
Boulla, G.2
Revy, P.3
Lemaitre, F.4
Trautmann, A.5
-
26
-
-
85117877561
-
Resetting circulating CD8 T-cells to a tissue-like status permits sensitive detection of their responses to virus-and tumorassociated antigens
-
Mainz, Germany, 6-8 May
-
Fischer, J.; Hackenberg, S.; Grigoleit, G. U.; Stevanovic, S.; Huenig, T. Resetting circulating CD8 T-cells to a tissue-like status permits sensitive detection of their responses to virus-and tumorassociated antigens. Proceedings of the 12th Annual Meeting of the Association for Immunotherapy of Cancer, Mainz, Germany, 6-8 May 2014.
-
(2014)
Proceedings of the 12th Annual Meeting of the Association for Immunotherapy of Cancer
-
-
Fischer, J.1
Hackenberg, S.2
Grigoleit, G.U.3
Stevanovic, S.4
Huenig, T.5
-
27
-
-
84870625724
-
Resting of Cryopreserved PBMC Does Not Generally Benefit the Performance of Antigen-Specific T-cell ELISPOT Assays
-
Kuerten, S.; Batoulis, H.; Recks, M. S.; Karacsony, E.; Zhang, W.; Subbramanian, R. A.; Lehmann, P. V. Resting of Cryopreserved PBMC Does Not Generally Benefit the Performance of Antigen-Specific T-cell ELISPOT Assays. Cells 2012, 1, 409-427.
-
(2012)
Cells
, vol.1
, pp. 409-427
-
-
Kuerten, S.1
Batoulis, H.2
Recks, M.S.3
Karacsony, E.4
Zhang, W.5
Subbramanian, R.A.6
Lehmann, P.V.7
-
28
-
-
33748879767
-
Statistical positivity criteria for the analysis of ELISPOT assay data in HIV-1 vaccine trials
-
Moodie, Z.; Huang, Y.; Gu, L.; Hural, J.; Self, S. G. Statistical positivity criteria for the analysis of ELISPOT assay data in HIV-1 vaccine trials. J. Immunol. Methods 2006, 315, 121-132.
-
(2006)
J. Immunol. Methods
, vol.315
, pp. 121-132
-
-
Moodie, Z.1
Huang, Y.2
Gu, L.3
Hural, J.4
Self, S.G.5
-
29
-
-
4344560286
-
A modified human ELISPOT assay to detect specific responses to primary tumor cell targets
-
Malyguine, A.; Strobl, S. L.; Shafer-Weaver, K. A.; Ulderich, T.; Troke, A.; Baseler, M.; Kwak, L. W.; Neelapu, S. S. A modified human ELISPOT assay to detect specific responses to primary tumor cell targets. J. Translat. Med. 2004, 2, 9.
-
(2004)
J. Translat. Med
, vol.2
, pp. 9
-
-
Malyguine, A.1
Strobl, S.L.2
Shafer-Weaver, K.A.3
Ulderich, T.4
Troke, A.5
Baseler, M.6
Kwak, L.W.7
Neelapu, S.S.8
-
30
-
-
78650779470
-
Equivalence of ELISPOT assays demonstrated between major HIV network laboratories
-
Gill, D. K.; Huang, Y.; Levine, G. L.; Sambor, A.; Carter, D. K.; Sato, A.; Kopycinski, J.; Hayes, P.; Hahn, B.; Birungi, J.; et al. Equivalence of ELISPOT assays demonstrated between major HIV network laboratories. PLoS One. 2010, 5, e14330.
-
(2010)
PLoS One
, vol.5
-
-
Gill, D.K.1
Huang, Y.2
Levine, G.L.3
Sambor, A.4
Carter, D.K.5
Sato, A.6
Kopycinski, J.7
Hayes, P.8
Hahn, B.9
Birungi, J.10
-
31
-
-
37249087254
-
Safety and immunogenicity of cytotoxic T-lymphocyte poly-epitope, DNA plasmid (EP HIV-1090) vaccine in healthy, human immunodeficiency virus type 1 (HIV-1)-uninfected adults
-
Gorse, G. J.; Baden, L. R.; Wecker, M.; Newman, M. J.; Ferrari, G.; Weinhold, K. J.; Livingston, B. D.; Villafana, T. L.; Li, H.; Noonan, E.; Russell, N. D. Safety and immunogenicity of cytotoxic T-lymphocyte poly-epitope, DNA plasmid (EP HIV-1090) vaccine in healthy, human immunodeficiency virus type 1 (HIV-1)-uninfected adults. Vaccine 2008, 26, 215-223.
-
(2008)
Vaccine
, vol.26
, pp. 215-223
-
-
Gorse, G.J.1
Baden, L.R.2
Wecker, M.3
Newman, M.J.4
Ferrari, G.5
Weinhold, K.J.6
Livingston, B.D.7
Villafana, T.L.8
Li, H.9
Noonan, E.10
Russell, N.D.11
-
32
-
-
84939876111
-
Harmonisation of short-term in vitro culture for the expansion of antigen-specific CD8 T-cells with detection by ELISPOT and hla-multimer staining
-
Chudley, L.; McCann, K. J.; Coleman, A.; Cazaly, A. M.; Bidmon, N.; Britten, C. M.; van der Burg, S. H.; Gouttefangeas, C.; Jandus, C.; Laske, K.; et al. Harmonisation of short-term in vitro culture for the expansion of antigen-specific CD8 T-cells with detection by ELISPOT and hla-multimer staining. Cancer Immunol. Immunother. 2014, 63, 1199-1211.
-
(2014)
Cancer Immunol. Immunother
, vol.63
, pp. 1199-1211
-
-
Chudley, L.1
McCann, K.J.2
Coleman, A.3
Cazaly, A.M.4
Bidmon, N.5
Britten, C.M.6
van der Burg, S.H.7
Gouttefangeas, C.8
Jandus, C.9
Laske, K.10
-
33
-
-
84886698315
-
Innate and adaptive immune cells in the tumor microenvironment
-
Gajewski, T. F.; Schreiber, H.; Fu, Y. X. Innate and adaptive immune cells in the tumor microenvironment. Nat. Immunol. 2013, 14, 1014-1022.
-
(2013)
Nat. Immunol
, vol.14
, pp. 1014-1022
-
-
Gajewski, T.F.1
Schreiber, H.2
Fu, Y.X.3
|