Analytical and toxicological evaluation of flavor chemicals in electronic cigarette refill fluids

Scientific Reports

Volumn 8, Issue 1, 2018, Pages

  Behar, Rachel Z ^a   Luo, Wentai ^b   McWhirter, Kevin J ^b   Pankow, James F ^b   Talbot, Prue ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b Department of Electrical and Computer Engineering   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACROLEIN; CINNAMALDEHYDE; FLAVORING AGENT; NICOTINE; SOLVENT;

AEROSOL; CELL CULTURE; CELL LINE; CHEMISTRY; DRUG EFFECT; ELECTRONIC CIGARETTE; HUMAN; LUNG; MASS FRAGMENTOGRAPHY;

ACROLEIN; AEROSOLS; CELL LINE; CELLS, CULTURED; ELECTRONIC NICOTINE DELIVERY SYSTEMS; FLAVORING AGENTS; GAS CHROMATOGRAPHY-MASS SPECTROMETRY; HUMANS; LUNG; NICOTINE; SOLVENTS;

EID: 85047850720     PISSN: None     EISSN: 20452322     Source Type: Journal    
DOI: 10.1038/s41598-018-25575-6     Document Type: Article

References (34)

1. Zhu, S.-H. et al. Four hundred and sixty brands of e-cigarettes and counting: implications for product regulation. Tob Control 23(Suppl 3), iii3-9 (2014).
2. Tierney, P. A., Karpinski, C. D., Brown, J. E., Luo, W. & Pankow, J. F. Flavour chemicals in electronic cigarette fluids. Tob Control 25, e10-5 (2016).
3. Safety Assessment and Regulatory Authority to Use Flavors-Focus on Electronic Nicotine Delivery Systems and Flavored Tobacco Products. Available at: https://www.femaflavor.org/safety-Assessment-And-regulatory-Authority-use-flavors-focus-electronic-nicotine-delivery-systems (The Flavor and Extract Manufacturers Association of the United States, 2013).
4. Bahl, V. et al. Comparison of electronic cigarette refill fluid cytotoxicity using embryonic and adult models. Reprod Toxicol 34, 529-537 (2012).
5. Behar, R. Z. et al. Identification of toxicants in cinnamon-flavored electronic cigarette refill fluids. Toxicol in Vitro 28, 198-208 (2014).
6. Behar, R. Z., Wang, Y. & Talbot, P. Comparing the cytotoxicity of electronic cigarette fluids, aerosols and solvents. Tob Control tobaccocontrol-2016-053472, https://doi.org/10.1136/tobaccocontrol-2016-053472 (2017).
7. The Good Scents Company Information System. The Good Scents Company Information System Available at: http://www.thegoodscentscompany.com (Accessed: 2017).
8. Biological evaluation of medical devices-Part 5: Tests for in vitro cytotoxicity. ISO 10993-5:2009(E), iv-34 (2009).
9. Davis, B., Dang, M., Kim, J. & Talbot, P. Nicotine concentrations in electronic cigarette refill and do-it-yourself fluids. Nicotine Tob Res 17, 134-141 (2015).
10. Etter, J. F. Characteristics of users and usage of different types of electronic cigarettes: findings from an online survey. Addiction 111, 724-733 (2016).
11. Farsalinos, K. E., Romagna, G., Tsiapras, D., Kyrzopoulos, S. & Voudris, V. Characteristics, perceived side effects and benefits of electronic cigarette use: A worldwide survey of more than 19,000 consumers. IJERPH 11, 4356-4373 (2014).
12. Williams, M., Villarreal, A., Bozhilov, K., Lin, S. & Talbot, P. Metal and silicate particles including nanoparticles are present in electronic cigarette cartomizer fluid and aerosol. PLoS ONE 8, e57987-11 (2013).
13. Farsalinos, K. E., Kistler, K. A., Gillman, G. & Voudris, V. Evaluation of electronic cigarette liquids and aerosol for the presence of selected inhalation toxins. Nicotine Tob Res 17, 168-174 (2015).
14. Jensen, R. P., Strongin, R. M. & Peyton, D. H. Solvent Chemistry in the Electronic Cigarette Reaction Vessel. Sci Rep 7, 42549 (2017).
15. Nef, J. U. Dissociation processes in the glycol-glycerine sequence. Justus Liebigs Ann Chem 335, 191-245 (1904).
16. Jensen, R. P., Luo, W., Pankow, J. F., Strongin, R. M. & Peyton, D. H. Hidden formaldehyde in e-cigarette aerosols. N Engl J Med 372, 392-394 (2015).
17. Kosmider, L. et al. Carbonyl Compounds in Electronic Cigarette Vapors-Effects of Nicotine Solvent and Battery Output Voltage. Nicotine Tob Res 16, 1319-1326 (2014).
18. Sleiman, M. et al. Emissions from Electronic Cigarettes: Key Parameters Affecting the Release of Harmful Chemicals. Environ Sci Technol 50, 9644-9651 (2016).
19. Allen, J. G. et al. Flavoring Chemicals in E-Cigarettes: Diacetyl, 2,3-Pentanedione, and Acetoin in a Sample of 51 Products, Including Fruit-, Candy-, and Cocktail-Flavored E-Cigarettes. EHP 124, 733-739 (2016).
20. Behar, R. Z. et al. Distribution, quantification and toxicity of cinnamaldehyde in electronic cigarette refill fluids and aerosols. Tob Control 25(Suppl 2), ii94-ii102 (2016).
21. Gerloff, J. et al. Inflammatory Response and Barrier Dysfunction by Different e-Cigarette Flavoring Chemicals Identified by Gas Chromatography-Mass Spectrometry in e-Liquids and e-Vapors on Human Lung Epithelial Cells and Fibroblasts. Appl In Vitro Toxicol 3, 28-40 (2017).
22. Harber, P., Saechao, K. & Boomus, C. Diacetyl-induced lung disease. Toxicol Rev 25, 261-272 (2006).
23. Robinson, R. J., Hensel, E. C., Morabito, P. N. & Roundtree, K. A. Electronic Cigarette Topography in the Natural Environment. PLoS ONE 10, e0129296 (2015).
24. Robinson, R. J. et al. Week Long Topography Study of Young Adults Using Electronic Cigarettes in Their Natural Environment. PLoS ONE 11, e0164038 (2016).
25. Dautzenberg, B., & Bricard, D. Real-Time characterization of e-cigarettes use: The 1 million puffs study. J Addict Res Ther 6, https://doi.org/10.4172/2155-6105.1000229 (2015).
26. Clapp, P. W. et al. Flavored e-cigarette liquids and cinnamaldehyde impair respiratory innate immune cell function. Am J Physiol Lung Cell Mol Physiol 313, L278-L292 (2017).
27. Kosmider, L. et al. Cherry-flavoured electronic cigarettes expose users to the inhalation irritant, benzaldehyde. Thorax 71, 376-377 (2016).
28. Rowell, T. R. et al. Flavored e-cigarette liquids reduce proliferation and viability in the CALU3 airway epithelial cell line. Am J Physiol Lung Cell Mol Physiol 313, L52-L66 (2017).
29. Hutzler, C. et al. Chemical hazards present in liquids and vapors of electronic cigarettes. Arch Toxicol 88, 1295-1308 (2014).
30. Leigh, N. J., Lawton, R. I., Hershberger, P. A. & Goniewicz, M. L. Flavourings significantly affect inhalation toxicity of aerosol generated from electronic nicotine delivery systems (ENDS). Tob Control 25(Suppl 2), ii81-ii87 (2016).
31. Sherwood, C. L. & Boitano, S. Airway epithelial cell exposure to distinct e-cigarette liquid flavorings reveals toxicity thresholds and activation of CFTR by the chocolate flavoring 2,5-dimethypyrazine. Respir Res 17, 1-11 (2016).
32. Chu, L. H. & Chen, B.-S. Comparisons of robustness and sensitivity between cancer and normal cells by microarray data. Cancer Inform 6, 165-181 (2008).
33. Hua, M., Yip, H. & Talbot, P. Mining data on usage of electronic nicotine delivery systems (ENDS) from YouTube videos. Tob Control 22, 103-106 (2013).
34. Behar, R. Z. et al. A method for rapid dose-response screening of environmental chemicals using human embryonic stem cells. J Pharmacol Toxicol Methods 66, 238-245 (2012).