Combinatorial pathway engineering using type I-E CRISPR interference

Biotechnology and Bioengineering

Volumn 115, Issue 7, 2018, Pages 1878-1883

  Tarasava, Katia ^a   Liu, Rongming ^b   Garst, Andrew ^c   Gill, Ryan T ^a,b  

^a UCB 450   (United States)

^b UNIVERSITY OF COLORADO   (United States)

^c Muse Biotechnology Inc   (United States)

Author keywords

3 hydroxypropionic acid; combinatorial gene expression; CRISPR interference; malonyl coA; metabolic flux engineering

Indexed keywords

COBALT COMPOUNDS; ESCHERICHIA COLI; ITERATIVE METHODS; METABOLIC ENGINEERING; METABOLISM;

3-HYDROXYPROPIONIC ACID; COMPLEX METABOLIC NETWORKS; MALONYL-COA; METABOLIC FLUX; PATHWAY ENGINEERINGS; RATE-LIMITING STEPS; THREE ORDERS OF MAGNITUDE; TRIAL-AND-ERROR APPROACH;

GENE EXPRESSION;

BACTERIAL RNA; GUIDE RNA; HYDRACRYLIC ACID; MALONYL COENZYME A; LACTIC ACID;

ACKA GENE; ADHE GENE; ARTICLE; BACTERIAL GENE; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; COMBINATORIAL LIBRARY; CRISPR CAS SYSTEM; ESCHERICHIA COLI; ESSENTIAL GENE; FABI GENE; GENE KNOCKDOWN; GENE REPRESSION; LDHA GENE; MCR GENE; METABOLIC ENGINEERING; MGSA GENE; MOLECULAR CLONING; NONHUMAN; PFLB GENE; POXB GENE; PROTEIN EXPRESSION LEVEL; PROTEOMICS; PTA GENE; ANALOGS AND DERIVATIVES; GENE EXPRESSION REGULATION; GENETIC RECOMBINATION; GENETIC VARIATION; GENETICS; METABOLISM; PROCEDURES;

CRISPR-CAS SYSTEMS; ESCHERICHIA COLI; GENE EXPRESSION REGULATION, BACTERIAL; GENETIC VARIATION; LACTIC ACID; MALONYL COENZYME A; METABOLIC ENGINEERING; METABOLIC NETWORKS AND PATHWAYS; RECOMBINATION, GENETIC;

EID: 85044546572     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.26589     Document Type: Article

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