Elucidating the Burden of HIV in Tissues Using Multiplexed Immunofluorescence and In Situ Hybridization: Methods for the Single-Cell Phenotypic Characterization of Cells Harboring HIV In Situ

Journal of Histochemistry and Cytochemistry

Volumn 66, Issue 6, 2018, Pages 427-446

  Vasquez, Joshua J ^a   Hussien, Rajaa ^a   Aguilar Rodriguez, Brandon ^a   Junger, Henrik ^a   Dobi, Dejan ^a   Henrich, Timothy J ^a   Thanh, Cassandra ^a   Gibson, Erica ^a   Hogan, Louise E ^a   McCune, Joseph ^a   Hunt, Peter W ^a   Stoddart, Cheryl A ^a   Laszik, Zoltan G ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

HIV reservoir; quantitative image analysis; tissue fixation

Indexed keywords

CD3 ANTIGEN; FLUORESCENT DYE; PARAFFIN; VIRUS DNA; VIRUS RNA;

ACH-2 CELL LINE; ARTICLE; AUTOFLUORESCENCE; CONTROLLED STUDY; DIGITAL IMAGING; HUMAN; HUMAN IMMUNODEFICIENCY VIRUS; HUMAN TISSUE; IMMUNOFLUORESCENCE; IN SITU HYBRIDIZATION; PHASE SEPARATION; PHENOTYPE; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; RNA ISOLATION; SIGNAL DETECTION; SINGLE CELL ANALYSIS; FLUORESCENT ANTIBODY TECHNIQUE; GENETICS; HUMAN IMMUNODEFICIENCY VIRUS INFECTION; ISOLATION AND PURIFICATION; PATHOLOGY; PROCEDURES; TISSUE FIXATION; VIROLOGY; VIRUS LOAD;

DNA, VIRAL; FLUORESCENT ANTIBODY TECHNIQUE; HIV; HIV INFECTIONS; HUMANS; IN SITU HYBRIDIZATION; PARAFFIN EMBEDDING; RNA, VIRAL; SINGLE-CELL ANALYSIS; TISSUE FIXATION; VIRAL LOAD;

EID: 85044032316     PISSN: 00221554     EISSN: 15515044     Source Type: Journal    
DOI: 10.1369/0022155418756848     Document Type: Article

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